Standard name | |
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Description | mRNA transport regulator; essential nuclear protein; Mex67p and Mtr2p form a mRNA export complex which binds to RNA |
ensLOC | DeepLoc | |||||||||||||||||||||||
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Localization | WT1 | WT2 | WT3 | RAP60 | RAP140 | RAP220 | RAP300 | RAP380 | RAP460 | RAP540 | RAP620 | RAP700 | HU80 | HU120 | HU160 | rpd3Δ_1 | rpd3Δ_2 | rpd3Δ_3 | WT1 | WT2 | WT3 | AF100 | AF140 | AF180 |
Cortical Patches | – | 0 | 0 | 0 | 1 | 0 | 0 | – | 0 | 0 | – | 0 | 0 | 2 | 1 | 1 | 1 | 1 | 0 | 0 | 0 | 2 | 3 | 0 |
Bud | – | 0 | 0 | 0 | 1 | 0 | 0 | – | 0 | 0 | – | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 1 | 0 | 1 | 5 | 6 | 5 |
Bud Neck | – | 0 | 0 | 0 | 0 | 0 | 0 | – | 0 | 0 | – | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
Bud Site | – | 0 | 0 | 0 | 0 | 0 | 0 | – | 0 | 0 | – | 1 | 0 | 0 | 0 | 0 | 0 | 0 | – | – | – | – | – | – |
Cell Periphery | – | 0 | 0 | 1 | 0 | 0 | 0 | – | 0 | 0 | – | 0 | 0 | 0 | 2 | 1 | 0 | 1 | 0 | 0 | 0 | 0 | 0 | 0 |
Cytoplasm | – | 59 | 9 | 5 | 4 | 13 | 39 | – | 31 | 18 | – | 11 | 13 | 41 | 56 | 29 | 30 | 28 | 15 | 61 | 5 | 1 | 4 | 4 |
Endoplasmic Reticulum | – | 0 | 1 | 1 | 0 | 1 | 3 | – | 3 | 0 | – | 3 | 8 | 8 | 12 | 4 | 3 | 3 | 1 | 3 | 0 | 6 | 8 | 5 |
Endosome | – | 0 | 3 | 2 | 1 | 2 | 11 | – | 2 | 5 | – | 4 | 9 | 20 | 22 | 0 | 1 | 0 | 2 | 0 | 6 | 13 | 28 | 24 |
Golgi | – | 0 | 2 | 1 | 3 | 0 | 2 | – | 0 | 0 | – | 0 | 4 | 16 | 12 | 5 | 12 | 15 | 0 | 0 | 1 | 5 | 10 | 5 |
Mitochondria | – | 0 | 0 | 0 | 0 | 15 | 2 | – | 22 | 8 | – | 17 | 0 | 0 | 0 | 2 | 1 | 0 | 2 | 0 | 0 | 2 | 2 | 2 |
Nucleus | – | 0 | 2 | 0 | 1 | 3 | 3 | – | 14 | 3 | – | 4 | 3 | 0 | 0 | 0 | 1 | 0 | 1 | 4 | 3 | 7 | 16 | 13 |
Nuclear Periphery | – | 0 | 10 | 3 | 4 | 8 | 18 | – | 22 | 3 | – | 24 | 24 | 33 | 16 | 2 | 1 | 3 | 10 | 3 | 67 | 37 | 119 | 73 |
Nucleolus | – | 0 | 0 | 0 | 0 | 3 | 3 | – | 4 | 3 | – | 6 | 10 | 8 | 6 | 1 | 2 | 3 | 0 | 1 | 2 | 6 | 10 | 10 |
Peroxisomes | – | 0 | 0 | 0 | 0 | 0 | 0 | – | 0 | 0 | – | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
SpindlePole | – | 0 | 0 | 0 | 0 | 0 | 0 | – | 0 | 1 | – | 0 | 0 | 1 | 0 | 0 | 0 | 0 | 0 | 0 | 1 | 1 | 4 | 4 |
Vac/Vac Membrane | – | 1 | 48 | 12 | 7 | 12 | 54 | – | 32 | 13 | – | 25 | 90 | 190 | 186 | 72 | 89 | 73 | 0 | 1 | 8 | 16 | 48 | 54 |
Unique Cell Count | 60 | 66 | 21 | 16 | 48 | 120 | 115 | 49 | 75 | 128 | 258 | 267 | 96 | 117 | 105 | 38 | 83 | 102 | 109 | 269 | 209 | |||
Labelled Cell Count | 60 | 75 | 25 | 22 | 57 | 135 | 130 | 54 | 95 | 161 | 319 | 313 | 117 | 141 | 127 | 38 | 83 | 102 | 109 | 269 | 209 |
Screen | WT1 | WT2 | WT3 | RAP60 | RAP140 | RAP220 | RAP300 | RAP380 | RAP460 | RAP540 | RAP620 | RAP700 | HU80 | HU120 | HU160 | rpd3Δ_1 | rpd3Δ_2 | rpd3Δ_3 | AF100 | AF140 | AF180 |
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Mean Cell GFP Intensity (1e-4) | – | 9.4 | 13.7 | 12.6 | 11.9 | 6.8 | 10.0 | – | 8.3 | 8.6 | – | 8.2 | 14.7 | 15.4 | 14.8 | 19.5 | 19.8 | 17.7 | 12.0 | 13.4 | 13.7 |
Std Deviation (1e-4) | – | 1.6 | 2.5 | 2.6 | 2.8 | 2.2 | 2.7 | – | 2.3 | 2.2 | – | 2.5 | 3.0 | 3.0 | 2.7 | 4.6 | 4.7 | 5.1 | 2.2 | 2.3 | 2.6 |
Intensity Change (Log2) | – | – | – | -0.12 | -0.2 | -1.0 | -0.46 | – | -0.73 | -0.67 | – | -0.74 | 0.1 | 0.17 | 0.11 | 0.51 | 0.53 | 0.37 | -0.19 | -0.03 | 0.0 |
Localization | RAP60 | RAP140 | RAP220 | RAP300 | RAP380 | RAP460 | RAP540 | RAP620 | RAP700 | HU80 | HU120 | HU160 | rpd3Δ_1 | rpd3Δ_2 | rpd3Δ_3 |
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Cortical Patches | 0 | 0 | 0 | 0 | – | 0 | 0 | – | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
Bud | 0 | 0 | 0 | 0 | – | 0 | 0 | – | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
Bud Neck | 0 | 0 | 0 | 0 | – | 0 | 0 | – | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
Bud Site | 0 | 0 | 0 | 0 | – | 0 | 0 | – | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
Cell Periphery | 0 | 0 | 0 | 0 | – | 0 | 0 | – | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
Cytoplasm | 0 | 0 | 1.8 | 2.8 | – | 2.1 | 2.9 | – | 0.2 | -0.7 | 0.5 | 1.3 | 2.4 | 1.9 | 2.0 |
Endoplasmic Reticulum | 0 | 0 | 0 | 0 | – | 0 | 0 | – | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
Endosome | 0 | 0 | 0 | 0 | – | 0 | 0 | – | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
Golgi | 0 | 0 | 0 | 0 | – | 0 | 0 | – | 0 | 0 | 0 | 0 | 0 | 1.8 | 2.4 |
Mitochondria | 0 | 0 | 4.9 | 0 | – | 3.8 | 0 | – | 4.1 | 0 | 0 | 0 | 0 | 0 | 0 |
Nucleus | 0 | 0 | 0 | 0 | – | 2.1 | 0 | – | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
Nuclear Periphery | 0 | 0 | 0.2 | 0 | – | 0.7 | -1.5 | – | 2.3 | 0.6 | -0.5 | -2.5 | 0 | 0 | -3.0 |
Nucleolus | 0 | 0 | 0 | 0 | – | 0 | 0 | – | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
Peroxisomes | 0 | 0 | 0 | 0 | – | 0 | 0 | – | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
SpindlePole | 0 | 0 | 0 | 0 | – | 0 | 0 | – | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
Vacuole | -1.3 | -2.2 | -5.0 | -3.6 | – | -5.9 | -4.9 | – | -4.7 | -0.4 | 0.2 | -0.5 | 0.3 | 0.5 | -0.4 |
Temp | Actin Patch (Sac6-tdTomato) | Cortical Patch (Sla1-GFP) | Late Endosome (Snf7-GFP) | Vacuole (Vph1-GFP) |
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37℃ | ||||
RT |
Gene / Allele | Actin Patch (Sac6-tdTomato) | Cortical Patch (Sla1-GFP) | Late Endosome (Snf7-GFP) | Vacuole (Sac6-tdTomato) |
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Gene | Images |
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Gene | Images |
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