Standard name
Description mRNA transport regulator; essential nuclear protein; Mex67p and Mtr2p form a mRNA export complex which binds to RNA
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Micrographs

ensLOC DeepLoc
Localization WT1 WT2 WT3 RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3 WT1 WT2 WT3 AF100 AF140 AF180
Cortical Patches – 0 0 0 0.06 0 0 – 0 0 – 0 0 0 0 0 0 0 0 0 0 0 0 0
Bud – 0 0 0 0.06 0 0 – 0 0 – 0 0 0 0 0 0 0 0 0 0 0.05 0 0
Bud Neck – 0 0 0 0 0 0 – 0 0 – 0 0 0 0 0 0 0 0 0 0 0 0 0
Bud Site – 0 0 0 0 0 0 – 0 0 – 0 0 0 0 0 0 0 – – – – – –
Cell Periphery – 0 0 0.05 0 0 0 – 0 0 – 0 0 0 0 0 0 0 0 0 0 0 0 0
Cytoplasm – 0.98 0.14 0.24 0.25 0.27 0.33 – 0.27 0.37 – 0.15 0.1 0.16 0.21 0.3 0.26 0.27 0.41 0.74 0.05 0 0 0
Endoplasmic Reticulum – 0 0 0.05 0 0 0 – 0 0 – 0 0.06 0 0 0 0 0 0 0 0 0.06 0 0
Endosome – 0 0.05 0.1 0.06 0 0.09 – 0 0.1 – 0.05 0.07 0.08 0.08 0 0 0 0.07 0 0.07 0.12 0.11 0.12
Golgi – 0 0 0.05 0.19 0 0 – 0 0 – 0 0 0.06 0 0.05 0.1 0.14 0 0 0 0.05 0 0
Mitochondria – 0 0 0 0 0.31 0 – 0.19 0.16 – 0.23 0 0 0 0 0 0 0.06 0 0 0 0 0
Nucleus – 0 0 0 0.06 0.06 0 – 0.12 0.06 – 0.05 0 0 0 0 0 0 0 0.06 0 0.07 0.06 0.07
Nuclear Periphery – 0 0.15 0.14 0.25 0.17 0.15 – 0.19 0.06 – 0.32 0.19 0.13 0.06 0 0 0 0.29 0.05 0.66 0.34 0.44 0.35
Nucleolus – 0 0 0 0 0.06 0 – 0 0.06 – 0.08 0.08 0 0 0 0 0 0 0 0 0.06 0 0.05
Peroxisomes – 0 0 0 0 0 0 – 0 0 – 0 0 0 0 0 0 0 0 0 0 0 0 0
SpindlePole – 0 0 0 0 0 0 – 0 0 – 0 0 0 0 0 0 0 0 0 0 0 0 0
Vac/Vac Membrane – 0 0.73 0.57 0.44 0.25 0.45 – 0.28 0.27 – 0.33 0.7 0.74 0.7 0.75 0.76 0.7 0 0 0.09 0.15 0.18 0.26
ensLOC DeepLoc
Localization WT1 WT2 WT3 RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3 WT1 WT2 WT3 AF100 AF140 AF180
Cortical Patches – 0 0 0 1 0 0 – 0 0 – 0 0 2 1 1 1 1 0 0 0 2 3 0
Bud – 0 0 0 1 0 0 – 0 0 – 0 0 0 0 0 0 0 1 0 1 5 6 5
Bud Neck – 0 0 0 0 0 0 – 0 0 – 0 0 0 0 0 0 0 0 0 0 0 0 0
Bud Site – 0 0 0 0 0 0 – 0 0 – 1 0 0 0 0 0 0 – – – – – –
Cell Periphery – 0 0 1 0 0 0 – 0 0 – 0 0 0 2 1 0 1 0 0 0 0 0 0
Cytoplasm – 59 9 5 4 13 39 – 31 18 – 11 13 41 56 29 30 28 15 61 5 1 4 4
Endoplasmic Reticulum – 0 1 1 0 1 3 – 3 0 – 3 8 8 12 4 3 3 1 3 0 6 8 5
Endosome – 0 3 2 1 2 11 – 2 5 – 4 9 20 22 0 1 0 2 0 6 13 28 24
Golgi – 0 2 1 3 0 2 – 0 0 – 0 4 16 12 5 12 15 0 0 1 5 10 5
Mitochondria – 0 0 0 0 15 2 – 22 8 – 17 0 0 0 2 1 0 2 0 0 2 2 2
Nucleus – 0 2 0 1 3 3 – 14 3 – 4 3 0 0 0 1 0 1 4 3 7 16 13
Nuclear Periphery – 0 10 3 4 8 18 – 22 3 – 24 24 33 16 2 1 3 10 3 67 37 119 73
Nucleolus – 0 0 0 0 3 3 – 4 3 – 6 10 8 6 1 2 3 0 1 2 6 10 10
Peroxisomes – 0 0 0 0 0 0 – 0 0 – 0 0 0 0 0 0 0 0 0 0 0 0 0
SpindlePole – 0 0 0 0 0 0 – 0 1 – 0 0 1 0 0 0 0 0 0 1 1 4 4
Vac/Vac Membrane – 1 48 12 7 12 54 – 32 13 – 25 90 190 186 72 89 73 0 1 8 16 48 54
Unique Cell Count 60 66 21 16 48 120 115 49 75 128 258 267 96 117 105 38 83 102 109 269 209
Labelled Cell Count 60 75 25 22 57 135 130 54 95 161 319 313 117 141 127 38 83 102 109 269 209


Nuclear Periphery

(Huh et al. Nature 2003 425:686)
Screen WT1 WT2 WT3 RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3 AF100 AF140 AF180
Mean Cell GFP Intensity (1e-4) – 9.4 13.7 12.6 11.9 6.8 10.0 – 8.3 8.6 – 8.2 14.7 15.4 14.8 19.5 19.8 17.7 12.0 13.4 13.7
Std Deviation (1e-4) – 1.6 2.5 2.6 2.8 2.2 2.7 – 2.3 2.2 – 2.5 3.0 3.0 2.7 4.6 4.7 5.1 2.2 2.3 2.6
Intensity Change (Log2) – – – -0.12 -0.2 -1.0 -0.46 – -0.73 -0.67 – -0.74 0.1 0.17 0.11 0.51 0.53 0.37 -0.19 -0.03 0.0


Localization RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3
Cortical Patches 0 0 0 0 – 0 0 – 0 0 0 0 0 0 0
Bud 0 0 0 0 – 0 0 – 0 0 0 0 0 0 0
Bud Neck 0 0 0 0 – 0 0 – 0 0 0 0 0 0 0
Bud Site 0 0 0 0 – 0 0 – 0 0 0 0 0 0 0
Cell Periphery 0 0 0 0 – 0 0 – 0 0 0 0 0 0 0
Cytoplasm 0 0 1.8 2.8 – 2.1 2.9 – 0.2 -0.7 0.5 1.3 2.4 1.9 2.0
Endoplasmic Reticulum 0 0 0 0 – 0 0 – 0 0 0 0 0 0 0
Endosome 0 0 0 0 – 0 0 – 0 0 0 0 0 0 0
Golgi 0 0 0 0 – 0 0 – 0 0 0 0 0 1.8 2.4
Mitochondria 0 0 4.9 0 – 3.8 0 – 4.1 0 0 0 0 0 0
Nucleus 0 0 0 0 – 2.1 0 – 0 0 0 0 0 0 0
Nuclear Periphery 0 0 0.2 0 – 0.7 -1.5 – 2.3 0.6 -0.5 -2.5 0 0 -3.0
Nucleolus 0 0 0 0 – 0 0 – 0 0 0 0 0 0 0
Peroxisomes 0 0 0 0 – 0 0 – 0 0 0 0 0 0 0
SpindlePole 0 0 0 0 – 0 0 – 0 0 0 0 0 0 0
Vacuole -1.3 -2.2 -5.0 -3.6 – -5.9 -4.9 – -4.7 -0.4 0.2 -0.5 0.3 0.5 -0.4


Endocytosis
Temp Actin Patch (Sac6-tdTomato) Cortical Patch (Sla1-GFP) Late Endosome (Snf7-GFP) Vacuole (Vph1-GFP)
37℃
RT
Cell Cycle Omics

CYCLoPs (Mtr2-GFP)
Gene / Allele Actin Patch (Sac6-tdTomato) Cortical Patch (Sla1-GFP) Late Endosome (Snf7-GFP) Vacuole (Sac6-tdTomato)
Gene Images
Gene Images
Images are not yet available
Images are not yet available