Standard name | |
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Description | Subunit IV of cytochrome c oxidase; the terminal member of the mitochondrial inner membrane electron transport chain; precursor N-terminal 25 residues are cleaved during mitochondrial import; phosphorylated; spermidine enhances translation |
ensLOC | DeepLoc | |||||||||||||||||||||||
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Localization | WT1 | WT2 | WT3 | RAP60 | RAP140 | RAP220 | RAP300 | RAP380 | RAP460 | RAP540 | RAP620 | RAP700 | HU80 | HU120 | HU160 | rpd3Δ_1 | rpd3Δ_2 | rpd3Δ_3 | WT1 | WT2 | WT3 | AF100 | AF140 | AF180 |
Cortical Patches | 0 | 2 | 0 | 1 | 2 | 4 | 3 | 6 | 4 | 3 | 8 | 8 | 2 | 2 | 5 | – | 1 | 2 | 1 | 13 | 4 | 11 | 23 | 27 |
Bud | 2 | 1 | 8 | 7 | 6 | 15 | 20 | 20 | 10 | 7 | 5 | 11 | 4 | 4 | 2 | – | 0 | 1 | 0 | 1 | 0 | 1 | 3 | 4 |
Bud Neck | 0 | 1 | 0 | 1 | 1 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | – | 0 | 0 | 0 | 0 | 0 | 1 | 1 | 0 |
Bud Site | 1 | 1 | 1 | 0 | 0 | 2 | 7 | 2 | 5 | 1 | 0 | 4 | 0 | 0 | 0 | – | 0 | 0 | – | – | – | – | – | – |
Cell Periphery | 2 | 10 | 1 | 6 | 0 | 1 | 2 | 0 | 1 | 2 | 0 | 1 | 13 | 17 | 14 | – | 0 | 3 | 0 | 0 | 0 | 0 | 0 | 0 |
Cytoplasm | 1 | 0 | 1 | 4 | 4 | 3 | 5 | 1 | 9 | 3 | 6 | 5 | 4 | 3 | 11 | – | 0 | 1 | 0 | 0 | 0 | 0 | 0 | 1 |
Endoplasmic Reticulum | 1 | 2 | 1 | 1 | 1 | 4 | 3 | 3 | 5 | 4 | 5 | 2 | 0 | 1 | 4 | – | 0 | 2 | 1 | 0 | 0 | 0 | 0 | 3 |
Endosome | 14 | 5 | 2 | 5 | 11 | 0 | 2 | 3 | 0 | 2 | 0 | 0 | 14 | 53 | 57 | – | 1 | 6 | 11 | 11 | 3 | 3 | 17 | 15 |
Golgi | 0 | 10 | 0 | 3 | 2 | 3 | 0 | 10 | 5 | 11 | 20 | 7 | 24 | 52 | 118 | – | 8 | 17 | 3 | 32 | 12 | 16 | 46 | 61 |
Mitochondria | 102 | 213 | 116 | 267 | 387 | 409 | 530 | 540 | 450 | 570 | 368 | 600 | 232 | 375 | 327 | – | 0 | 1 | 92 | 162 | 90 | 99 | 162 | 181 |
Nucleus | 0 | 0 | 0 | 0 | 1 | 2 | 3 | 1 | 2 | 0 | 0 | 1 | 0 | 1 | 2 | – | 1 | 0 | 0 | 0 | 1 | 0 | 1 | 2 |
Nuclear Periphery | 9 | 9 | 1 | 4 | 8 | 27 | 33 | 25 | 18 | 20 | 10 | 17 | 2 | 7 | 6 | – | 2 | 2 | 1 | 1 | 0 | 1 | 2 | 2 |
Nucleolus | 3 | 8 | 5 | 4 | 18 | 30 | 53 | 92 | 51 | 67 | 40 | 70 | 7 | 13 | 6 | – | 1 | 1 | 1 | 1 | 0 | 0 | 2 | 1 |
Peroxisomes | 9 | 7 | 10 | 29 | 20 | 5 | 8 | 10 | 11 | 14 | 1 | 8 | 25 | 66 | 48 | – | 2 | 3 | 11 | 15 | 13 | 18 | 29 | 30 |
SpindlePole | 2 | 3 | 1 | 1 | 2 | 0 | 5 | 6 | 3 | 4 | 0 | 4 | 1 | 15 | 7 | – | 0 | 1 | 0 | 0 | 1 | 1 | 1 | 1 |
Vac/Vac Membrane | 10 | 21 | 5 | 8 | 21 | 19 | 14 | 25 | 12 | 16 | 16 | 11 | 55 | 85 | 117 | – | 2 | 3 | 1 | 2 | 1 | 4 | 5 | 14 |
Unique Cell Count | 116 | 239 | 125 | 280 | 408 | 442 | 587 | 617 | 498 | 624 | 402 | 637 | 271 | 478 | 505 | 17 | 35 | 126 | 247 | 128 | 162 | 302 | 354 | |
Labelled Cell Count | 156 | 293 | 152 | 341 | 484 | 524 | 688 | 744 | 586 | 724 | 479 | 749 | 383 | 694 | 724 | 18 | 43 | 126 | 247 | 128 | 162 | 302 | 354 |
Screen | WT1 | WT2 | WT3 | RAP60 | RAP140 | RAP220 | RAP300 | RAP380 | RAP460 | RAP540 | RAP620 | RAP700 | HU80 | HU120 | HU160 | rpd3Δ_1 | rpd3Δ_2 | rpd3Δ_3 | AF100 | AF140 | AF180 |
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Mean Cell GFP Intensity (1e-4) | 6.2 | 10.5 | 10.2 | 10.5 | 12.4 | 13.1 | 14.4 | 17.7 | 19.3 | 21.9 | 24.1 | 24.4 | 10.0 | 10.4 | 10.3 | – | 15.7 | 17.4 | 14.9 | 14.0 | 13.7 |
Std Deviation (1e-4) | 1.7 | 2.8 | 5.0 | 5.4 | 5.4 | 5.3 | 5.8 | 7.3 | 8.5 | 10.1 | 11.7 | 11.6 | 5.1 | 6.3 | 4.9 | – | 5.4 | 6.0 | 15.3 | 10.1 | 9.9 |
Intensity Change (Log2) | – | – | – | 0.03 | 0.28 | 0.35 | 0.49 | 0.8 | 0.92 | 1.1 | 1.24 | 1.25 | -0.03 | 0.02 | 0.01 | – | 0.62 | 0.77 | 0.55 | 0.45 | 0.43 |
Localization | RAP60 | RAP140 | RAP220 | RAP300 | RAP380 | RAP460 | RAP540 | RAP620 | RAP700 | HU80 | HU120 | HU160 | rpd3Δ_1 | rpd3Δ_2 | rpd3Δ_3 |
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Cortical Patches | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | – | 0 | 0 |
Bud | 0 | 0 | -1.5 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | – | 0 | 0 |
Bud Neck | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | – | 0 | 0 |
Bud Site | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | – | 0 | 0 |
Cell Periphery | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | – | 0 | 0 |
Cytoplasm | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | – | 0 | 0 |
Endoplasmic Reticulum | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | – | 0 | 0 |
Endosome | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 1.7 | 3.3 | 3.3 | – | 0 | 0 |
Golgi | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 3.4 | 3.9 | 6.0 | – | 0 | 0 |
Mitochondria | 1.0 | 0.9 | -0.1 | -0.9 | -1.7 | -0.8 | -0.5 | -0.4 | 0.6 | -2.0 | -3.7 | -6.1 | – | -9.3 | -10.6 |
Nucleus | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | – | 0 | 0 |
Nuclear Periphery | 0 | 0 | 2.4 | 2.3 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | – | 0 | 0 |
Nucleolus | 0 | 0.2 | 1.1 | 1.9 | 3.3 | 2.2 | 2.3 | 2.1 | 2.4 | 0 | 0 | 0 | – | 0 | 0 |
Peroxisomes | 0.7 | -1.3 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0.4 | 1.7 | 0.5 | – | 0 | 0 |
SpindlePole | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | – | 0 | 0 |
Vacuole | 0 | 0.5 | 0.1 | 0 | 0 | 0 | 0 | 0 | 0 | 4.2 | 3.9 | 4.9 | – | 0 | 0 |
Temp | Actin Patch (Sac6-tdTomato) | Cortical Patch (Sla1-GFP) | Late Endosome (Snf7-GFP) | Vacuole (Vph1-GFP) |
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37℃ | ||||
RT |
Gene / Allele | Actin Patch (Sac6-tdTomato) | Cortical Patch (Sla1-GFP) | Late Endosome (Snf7-GFP) | Vacuole (Sac6-tdTomato) |
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Gene | Images |
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Gene | Images |
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