Standard name
Description Subunit IV of cytochrome c oxidase; the terminal member of the mitochondrial inner membrane electron transport chain; precursor N-terminal 25 residues are cleaved during mitochondrial import; phosphorylated; spermidine enhances translation
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Micrographs

ensLOC DeepLoc
Localization WT1 WT2 WT3 RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3 WT1 WT2 WT3 AF100 AF140 AF180
Cortical Patches 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 – 0.06 0.06 0 0.06 0 0.07 0.08 0.08
Bud 0 0 0.06 0 0 0 0 0 0 0 0 0 0 0 0 – 0 0 0 0 0 0 0 0
Bud Neck 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 – 0 0 0 0 0 0 0 0
Bud Site 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 – 0 0 – – – – – –
Cell Periphery 0 0 0 0 0 0 0 0 0 0 0 0 0.05 0 0 – 0 0.09 0 0 0 0 0 0
Cytoplasm 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 – 0 0 0 0 0 0 0 0
Endoplasmic Reticulum 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 – 0 0.06 0 0 0 0 0 0
Endosome 0.12 0 0 0 0 0 0 0 0 0 0 0 0.05 0.11 0.11 – 0.06 0.17 0.09 0.05 0 0 0.06 0
Golgi 0 0 0 0 0 0 0 0 0 0 0.05 0 0.09 0.11 0.23 – 0.47 0.49 0 0.13 0.09 0.1 0.15 0.17
Mitochondria 0.88 0.89 0.93 0.95 0.95 0.93 0.9 0.88 0.9 0.91 0.92 0.94 0.86 0.78 0.65 – 0 0 0.73 0.66 0.71 0.62 0.54 0.51
Nucleus 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 – 0.06 0 0 0 0 0 0 0
Nuclear Periphery 0.08 0 0 0 0 0.06 0.06 0 0 0 0 0 0 0 0 – 0.12 0.06 0 0 0 0 0 0
Nucleolus 0 0 0 0 0 0.07 0.09 0.15 0.1 0.11 0.1 0.11 0 0 0 – 0.06 0 0 0 0 0 0 0
Peroxisomes 0.08 0 0.08 0.1 0.05 0 0 0 0 0 0 0 0.09 0.14 0.1 – 0.12 0.09 0.09 0.06 0.1 0.11 0.1 0.09
SpindlePole 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 – 0 0 0 0 0 0 0 0
Vac/Vac Membrane 0.09 0.09 0 0 0.05 0 0 0 0 0 0 0 0.2 0.18 0.23 – 0.12 0.09 0 0 0 0 0 0
ensLOC DeepLoc
Localization WT1 WT2 WT3 RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3 WT1 WT2 WT3 AF100 AF140 AF180
Cortical Patches 0 2 0 1 2 4 3 6 4 3 8 8 2 2 5 – 1 2 1 13 4 11 23 27
Bud 2 1 8 7 6 15 20 20 10 7 5 11 4 4 2 – 0 1 0 1 0 1 3 4
Bud Neck 0 1 0 1 1 0 0 0 0 0 0 0 0 0 0 – 0 0 0 0 0 1 1 0
Bud Site 1 1 1 0 0 2 7 2 5 1 0 4 0 0 0 – 0 0 – – – – – –
Cell Periphery 2 10 1 6 0 1 2 0 1 2 0 1 13 17 14 – 0 3 0 0 0 0 0 0
Cytoplasm 1 0 1 4 4 3 5 1 9 3 6 5 4 3 11 – 0 1 0 0 0 0 0 1
Endoplasmic Reticulum 1 2 1 1 1 4 3 3 5 4 5 2 0 1 4 – 0 2 1 0 0 0 0 3
Endosome 14 5 2 5 11 0 2 3 0 2 0 0 14 53 57 – 1 6 11 11 3 3 17 15
Golgi 0 10 0 3 2 3 0 10 5 11 20 7 24 52 118 – 8 17 3 32 12 16 46 61
Mitochondria 102 213 116 267 387 409 530 540 450 570 368 600 232 375 327 – 0 1 92 162 90 99 162 181
Nucleus 0 0 0 0 1 2 3 1 2 0 0 1 0 1 2 – 1 0 0 0 1 0 1 2
Nuclear Periphery 9 9 1 4 8 27 33 25 18 20 10 17 2 7 6 – 2 2 1 1 0 1 2 2
Nucleolus 3 8 5 4 18 30 53 92 51 67 40 70 7 13 6 – 1 1 1 1 0 0 2 1
Peroxisomes 9 7 10 29 20 5 8 10 11 14 1 8 25 66 48 – 2 3 11 15 13 18 29 30
SpindlePole 2 3 1 1 2 0 5 6 3 4 0 4 1 15 7 – 0 1 0 0 1 1 1 1
Vac/Vac Membrane 10 21 5 8 21 19 14 25 12 16 16 11 55 85 117 – 2 3 1 2 1 4 5 14
Unique Cell Count 116 239 125 280 408 442 587 617 498 624 402 637 271 478 505 17 35 126 247 128 162 302 354
Labelled Cell Count 156 293 152 341 484 524 688 744 586 724 479 749 383 694 724 18 43 126 247 128 162 302 354


Mitochondria

(Huh et al. Nature 2003 425:686)
Screen WT1 WT2 WT3 RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3 AF100 AF140 AF180
Mean Cell GFP Intensity (1e-4) 6.2 10.5 10.2 10.5 12.4 13.1 14.4 17.7 19.3 21.9 24.1 24.4 10.0 10.4 10.3 – 15.7 17.4 14.9 14.0 13.7
Std Deviation (1e-4) 1.7 2.8 5.0 5.4 5.4 5.3 5.8 7.3 8.5 10.1 11.7 11.6 5.1 6.3 4.9 – 5.4 6.0 15.3 10.1 9.9
Intensity Change (Log2) – – – 0.03 0.28 0.35 0.49 0.8 0.92 1.1 1.24 1.25 -0.03 0.02 0.01 – 0.62 0.77 0.55 0.45 0.43


Localization RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3
Cortical Patches 0 0 0 0 0 0 0 0 0 0 0 0 – 0 0
Bud 0 0 -1.5 0 0 0 0 0 0 0 0 0 – 0 0
Bud Neck 0 0 0 0 0 0 0 0 0 0 0 0 – 0 0
Bud Site 0 0 0 0 0 0 0 0 0 0 0 0 – 0 0
Cell Periphery 0 0 0 0 0 0 0 0 0 0 0 0 – 0 0
Cytoplasm 0 0 0 0 0 0 0 0 0 0 0 0 – 0 0
Endoplasmic Reticulum 0 0 0 0 0 0 0 0 0 0 0 0 – 0 0
Endosome 0 0 0 0 0 0 0 0 0 1.7 3.3 3.3 – 0 0
Golgi 0 0 0 0 0 0 0 0 0 3.4 3.9 6.0 – 0 0
Mitochondria 1.0 0.9 -0.1 -0.9 -1.7 -0.8 -0.5 -0.4 0.6 -2.0 -3.7 -6.1 – -9.3 -10.6
Nucleus 0 0 0 0 0 0 0 0 0 0 0 0 – 0 0
Nuclear Periphery 0 0 2.4 2.3 0 0 0 0 0 0 0 0 – 0 0
Nucleolus 0 0.2 1.1 1.9 3.3 2.2 2.3 2.1 2.4 0 0 0 – 0 0
Peroxisomes 0.7 -1.3 0 0 0 0 0 0 0 0.4 1.7 0.5 – 0 0
SpindlePole 0 0 0 0 0 0 0 0 0 0 0 0 – 0 0
Vacuole 0 0.5 0.1 0 0 0 0 0 0 4.2 3.9 4.9 – 0 0


Endocytosis
Temp Actin Patch (Sac6-tdTomato) Cortical Patch (Sla1-GFP) Late Endosome (Snf7-GFP) Vacuole (Vph1-GFP)
37℃
RT
Cell Cycle Omics

CYCLoPs (Cox4-GFP)
Gene / Allele Actin Patch (Sac6-tdTomato) Cortical Patch (Sla1-GFP) Late Endosome (Snf7-GFP) Vacuole (Sac6-tdTomato)
Gene Images
Gene Images
Images are not yet available
Images are not yet available