Standard name
Description Peroxisomal cystathionine beta-lyase; converts cystathionine into homocysteine; may be redox regulated by Gto1p; involved in the release of the aromatic thiol 3-mercaptohexanol during wine fermentation
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Micrographs

ensLOC DeepLoc
Localization WT1 WT2 WT3 RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3 WT1 WT2 WT3 AF100 AF140 AF180
Cortical Patches 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Bud 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Bud Neck 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Bud Site 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 – – – – – –
Cell Periphery 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Cytoplasm 0.88 0.97 0.86 0.88 0.92 0.93 0.79 0.87 0.85 0.82 0.75 0.69 0.96 0.95 0.95 0.93 0.88 0.88 0.88 0.79 0.76 0.85 0.89 0.81
Endoplasmic Reticulum 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Endosome 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Golgi 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Mitochondria 0 0 0 0.12 0.2 0.18 0.33 0.29 0.35 0.34 0.5 0.52 0 0 0 0 0 0 0 0 0 0 0 0
Nucleus 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0.13 0.05 0 0 0
Nuclear Periphery 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Nucleolus 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Peroxisomes 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
SpindlePole 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Vac/Vac Membrane 0.13 0.07 0.12 0.07 0 0 0 0 0 0 0 0 0.08 0.07 0.06 0.11 0.13 0.2 0 0 0.07 0 0 0.05
ensLOC DeepLoc
Localization WT1 WT2 WT3 RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3 WT1 WT2 WT3 AF100 AF140 AF180
Cortical Patches 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 4
Bud 0 0 0 1 1 2 1 9 4 4 3 1 0 0 1 0 0 0 1 3 0 1 1 6
Bud Neck 0 0 0 0 0 0 0 1 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 2
Bud Site 0 0 0 0 0 0 0 0 0 0 1 0 0 0 0 0 0 0 – – – – – –
Cell Periphery 0 0 0 0 0 0 0 1 2 2 1 2 1 2 2 1 2 2 0 0 0 0 0 0
Cytoplasm 92 109 43 96 97 105 131 232 117 106 104 88 267 452 413 266 165 109 115 102 53 153 265 229
Endoplasmic Reticulum 0 0 0 0 1 0 0 0 0 1 1 1 1 3 0 8 5 3 0 0 1 1 3 2
Endosome 3 0 0 0 0 0 1 0 0 0 0 0 0 0 2 2 2 1 2 0 0 1 2 3
Golgi 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 1 0 0 0 0 0 0 0 1
Mitochondria 2 0 2 13 21 20 55 78 48 44 69 66 0 2 2 1 2 0 1 0 1 1 1 1
Nucleus 0 0 1 1 2 0 2 4 0 0 0 0 4 1 5 4 1 0 3 17 3 7 6 3
Nuclear Periphery 0 0 0 0 0 0 1 1 0 0 0 0 0 1 0 0 0 0 0 0 0 0 0 0
Nucleolus 0 0 0 0 0 0 0 2 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Peroxisomes 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
SpindlePole 0 0 0 0 0 0 0 0 0 0 2 0 0 0 0 0 0 0 0 0 0 1 2 2
Vac/Vac Membrane 14 8 6 8 1 0 1 6 1 0 1 2 23 31 26 32 25 25 3 4 5 4 6 14
Unique Cell Count 104 112 50 109 105 113 165 268 137 129 138 127 279 476 434 286 187 124 132 130 70 180 297 285
Labelled Cell Count 111 117 52 119 123 127 192 334 172 157 182 160 296 492 451 315 202 140 132 130 70 180 297 285


Cytoplasm, Nucleus

(Huh et al. Nature 2003 425:686)
Screen WT1 WT2 WT3 RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3 AF100 AF140 AF180
Mean Cell GFP Intensity (1e-4) 10.1 9.0 6.3 5.6 5.1 4.4 3.4 3.8 3.5 3.3 3.2 3.0 7.5 7.1 7.3 18.4 21.8 22.5 7.0 6.8 7.1
Std Deviation (1e-4) 2.8 2.1 1.3 1.3 1.3 1.1 1.1 1.5 1.4 1.6 1.4 1.5 1.6 1.5 1.8 4.0 5.0 5.2 1.8 1.6 1.5
Intensity Change (Log2) – – – -0.19 -0.32 -0.53 -0.91 -0.74 -0.87 -0.93 -0.99 -1.09 0.24 0.17 0.2 1.54 1.78 1.82 0.15 0.11 0.16


Localization RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3
Cortical Patches 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Bud 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Bud Neck 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Bud Site 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Cell Periphery 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Cytoplasm 0.4 1.3 1.4 -1.0 0.1 -0.1 -0.6 -1.6 -2.3 2.7 2.6 2.6 1.7 0.4 0.3
Endoplasmic Reticulum 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Endosome 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Golgi 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Mitochondria 0 2.6 0 0 0 0 0 0 0 0 0 0 0 0 0
Nucleus 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Nuclear Periphery 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Nucleolus 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Peroxisomes 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
SpindlePole 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Vacuole 0 0 0 0 0 0 0 0 0 0 0 0 -0.2 0.3 1.3

Images

Circle plot not available

Protein Concentration and Protein Localization Data

R1 R2 R3
G1 Pre-START G1 Post-START S/G2 Metaphase Anaphase Telophase G1 Pre-START G1 Post-START S/G2 Metaphase Anaphase Telophase G1 Pre-START G1 Post-START S/G2 Metaphase Anaphase Telophase
Concentration 5.9622 9.0039 7.8555 8.1774 9.8277 8.568 7.1483 8.8379 7.9492 8.9663 7.7462 8.022 10.6048 14.1022 11.0555 11.8647 13.0574 11.6658
Actin 0.1167 0.0008 0.0014 0.0031 0.0001 0.0019 0.0524 0.0019 0.0198 0.025 0.0005 0.0108 0.0004 0.1118 0.0144 0.0005 0.0003 0.0095
Bud 0.0014 0.0002 0.0003 0.0063 0.0001 0.0003 0.0006 0.0093 0.0003 0.0002 0.0002 0.0003 0.0002 0.0005 0.0005 0.0003 0.0001 0.0016
Bud Neck 0.002 0.0002 0.0063 0.0005 0.0007 0.002 0.002 0.0002 0.0028 0.0008 0.0007 0.0015 0.0001 0.0001 0.0004 0.0005 0.0005 0.0013
Bud Periphery 0.0023 0.0001 0.0002 0.0011 0 0.0003 0.0006 0.0087 0.0002 0.0001 0 0.0004 0 0.0004 0.0007 0.0001 0 0.001
Bud Site 0.0066 0.0007 0.0104 0.002 0.0001 0.0008 0.0046 0.0057 0.0008 0.0002 0.0001 0.0004 0.0001 0.003 0.0011 0.0006 0 0.0003
Cell Periphery 0.0006 0.0001 0.0001 0.0002 0 0.0001 0.0003 0.0001 0.0001 0.0001 0 0.0002 0 0 0.0001 0 0 0.0002
Cytoplasm 0.558 0.9621 0.9308 0.8456 0.9635 0.9024 0.733 0.9333 0.8424 0.8976 0.9593 0.8775 0.9861 0.8281 0.9165 0.9366 0.9795 0.92
Cytoplasmic Foci 0.0705 0.0018 0.0025 0.0067 0.0013 0.0058 0.0293 0.0083 0.0124 0.0038 0.0045 0.0108 0.0009 0.016 0.005 0.0058 0.0013 0.0032
Eisosomes 0.0008 0 0 0 0 0.0001 0.0003 0 0.0001 0.0003 0 0.0001 0 0.0001 0.0001 0 0 0.0001
Endoplasmic Reticulum 0.016 0.0019 0.0015 0.0052 0.0013 0.0065 0.0142 0.0021 0.0084 0.0058 0.003 0.0116 0.0011 0.0004 0.0022 0.0027 0.0023 0.0026
Endosome 0.0627 0.0005 0.0013 0.002 0.0006 0.0078 0.0628 0.0011 0.0117 0.0126 0.0017 0.0134 0.0002 0.002 0.01 0.008 0.0007 0.0035
Golgi 0.015 0.0001 0.0002 0.0004 0 0.0015 0.0124 0.0002 0.003 0.004 0.0001 0.0045 0 0.008 0.0167 0.0002 0 0.0037
Lipid Particles 0.0199 0.0001 0.0003 0.0002 0 0.0043 0.0105 0.0001 0.0007 0.0016 0 0.003 0 0.0014 0.0021 0.0002 0 0.0001
Mitochondria 0.009 0.0001 0.0002 0.001 0.0001 0.0042 0.0019 0.0004 0.0015 0.0012 0.0002 0.0021 0.0001 0.0014 0.0126 0.0003 0.0001 0.0116
None 0.0265 0.0049 0.005 0.0122 0.0013 0.003 0.0296 0.0105 0.04 0.0222 0.0088 0.0252 0.004 0.0005 0.002 0.0035 0.0014 0.002
Nuclear Periphery 0.015 0.0032 0.0059 0.0049 0.0032 0.0077 0.0087 0.0015 0.0061 0.0073 0.0016 0.0043 0.0008 0.0008 0.0026 0.0045 0.0021 0.0022
Nucleolus 0.0008 0 0.0003 0.0009 0 0.0006 0.0007 0.0001 0.0001 0.0001 0 0.0016 0 0 0.0001 0.0002 0 0
Nucleus 0.0196 0.0215 0.0314 0.0987 0.0259 0.0447 0.0153 0.0125 0.032 0.0142 0.0179 0.0243 0.0055 0.0037 0.0101 0.0294 0.0106 0.0357
Peroxisomes 0.0236 0.0001 0.0002 0.0002 0 0.0018 0.0042 0.0002 0.0152 0.0002 0.0001 0.0006 0 0.0208 0.0008 0.0001 0 0.0002
Punctate Nuclear 0.0133 0.0009 0.0007 0.0072 0.0005 0.0019 0.009 0.0027 0.0014 0.0014 0.0009 0.0054 0.0002 0.0008 0.0008 0.0019 0.0006 0.0005
Vacuole 0.0143 0.0005 0.0007 0.0013 0.001 0.0017 0.0065 0.0008 0.0008 0.0006 0.0004 0.0017 0.0002 0.0001 0.0007 0.004 0.0003 0.0004
Vacuole Periphery 0.0054 0.0001 0.0001 0.0005 0.0001 0.0005 0.0012 0.0001 0.0002 0.0007 0.0001 0.0004 0 0.0001 0.0005 0.0005 0.0001 0.0003

Sequencing Data

R1 R2
G1 Post-START S/G2 Metaphase Anaphase Telophase G1 Post-START S/G2 Metaphase Anaphase Telophase
Gene Expression 4.341 3.5278 3.1413 8.0482 2.0467 1.5777 3.567 4.0927 4.3984 3.6087
Translational Efficiency – – – – – – – – – –

Hit Data

Dataset Hit
Protein Abundance ✔
Protein Localization ✘
Gene Expression ✘
Translational Efficiency –