Standard name
Human Ortholog
Description Subunit of the RES complex; RES complex is required for nuclear pre-mRNA retention and splicing; involved in bud-site selection; diploid mutants display a unipolar budding pattern instead of the wild-type bipolar pattern due to a specific defect in MATa1 pre-mRNA splicing which leads to haploid gene expression in diploids
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Micrographs

ensLOC DeepLoc
Localization WT1 WT2 WT3 RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3 WT1 WT2 WT3 AF100 AF140 AF180
Cortical Patches 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Bud 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Bud Neck 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Bud Site 0 0 0 0 0 0 0 0 0 0 0.06 0.05 0 0 0 0 0 0 – – – – – –
Cell Periphery 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Cytoplasm 0 0 0.06 0.09 0.08 0.07 0.11 0.1 0.07 0 0 0 0.11 0.14 0.16 0.07 0.07 0.07 0 0 0 0 0 0
Endoplasmic Reticulum 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Endosome 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Golgi 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Mitochondria 0 0.14 0 0 0 0.1 0.41 0.15 0.51 0.31 0.38 0.41 0 0 0 0 0 0 0 0 0 0 0 0
Nucleus 0.96 0.94 0.9 0.88 0.9 0.86 0.75 0.8 0.76 0.84 0.84 0.84 0.86 0.85 0.81 0.91 0.9 0.85 0.95 0.9 0.87 0.82 0.73 0.58
Nuclear Periphery 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Nucleolus 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0.05
Peroxisomes 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
SpindlePole 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Vac/Vac Membrane 0 0 0.05 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0.05 0 0 0 0.1 0.12 0.2
ensLOC DeepLoc
Localization WT1 WT2 WT3 RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3 WT1 WT2 WT3 AF100 AF140 AF180
Cortical Patches 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 2 0 0 0
Bud 0 0 0 0 0 0 2 4 6 8 6 7 0 0 0 0 0 1 1 1 0 0 3 5
Bud Neck 0 0 0 0 0 0 0 0 1 1 0 0 0 0 0 0 0 0 0 0 0 0 0 1
Bud Site 0 0 0 3 1 3 7 2 10 14 18 21 0 0 0 1 0 0 – – – – – –
Cell Periphery 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Cytoplasm 2 3 13 16 19 19 32 34 19 13 8 13 19 39 51 20 17 18 0 0 4 1 7 9
Endoplasmic Reticulum 0 1 0 0 0 1 0 0 0 0 0 0 0 0 0 5 7 7 0 0 1 2 2 0
Endosome 0 1 2 1 0 0 0 0 0 0 0 0 1 0 1 1 0 1 0 1 1 0 3 7
Golgi 0 0 1 0 0 0 0 1 0 0 0 0 0 0 0 0 0 0 0 0 0 1 1 0
Mitochondria 6 24 1 3 4 27 116 55 145 103 114 160 4 4 2 13 8 4 0 4 6 0 3 7
Nucleus 137 164 197 151 216 229 210 286 217 282 256 325 152 246 268 270 226 232 135 161 195 136 190 167
Nuclear Periphery 1 0 5 1 4 1 1 7 4 3 2 5 2 3 6 4 3 0 0 0 0 1 1 3
Nucleolus 1 0 1 0 0 3 2 7 8 13 10 12 1 1 3 3 4 7 0 0 1 1 9 15
Peroxisomes 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
SpindlePole 0 0 0 0 1 0 3 4 1 0 0 2 0 0 5 1 1 3 0 0 0 1 2 6
Vac/Vac Membrane 3 2 10 3 5 9 0 11 1 1 3 7 3 4 7 8 6 15 3 6 7 17 32 57
Unique Cell Count 143 174 220 171 240 266 280 356 286 335 304 389 177 288 329 298 251 273 143 179 224 166 262 286
Labelled Cell Count 150 195 230 178 250 292 373 411 412 438 417 552 182 297 343 326 272 288 143 179 224 166 262 286


Cytoplasm, Nucleus

(Huh et al. Nature 2003 425:686)
Screen WT1 WT2 WT3 RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3 AF100 AF140 AF180
Mean Cell GFP Intensity (1e-4) 6.6 5.2 7.4 6.9 7.3 5.8 4.0 5.8 4.3 4.8 4.6 4.7 7.3 7.0 7.1 6.3 8.1 7.5 7.8 8.1 7.7
Std Deviation (1e-4) 1.2 0.9 2.1 1.9 2.3 1.4 1.5 1.8 1.3 1.2 1.1 1.1 2.1 1.9 2.1 1.3 2.0 1.8 2.0 2.0 2.0
Intensity Change (Log2) – – – -0.11 -0.04 -0.37 -0.88 -0.36 -0.78 -0.64 -0.7 -0.68 -0.03 -0.08 -0.08 -0.25 0.12 0.01 0.06 0.12 0.04


Localization RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3
Cortical Patches 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Bud 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Bud Neck 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Bud Site 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Cell Periphery 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Cytoplasm 1.3 0.8 0.5 2.1 1.6 0.3 -1.1 -1.9 -1.5 1.8 2.8 3.4 0.4 0.4 0.3
Endoplasmic Reticulum 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Endosome 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Golgi 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Mitochondria 0 0 4.6 0 5.9 0 0 0 0 0 0 0 2.7 0 0
Nucleus -0.4 0.2 -1.2 -4.1 -2.9 -4.0 -1.8 -1.8 -2.0 -1.1 -1.4 -2.6 0.4 0.2 -1.5
Nuclear Periphery 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Nucleolus 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Peroxisomes 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
SpindlePole 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Vacuole -1.5 -1.5 -0.7 0 -0.9 0 0 -2.6 -2.0 -1.6 -2.2 -1.6 -1.1 -1.3 0.5

Images

Circle plot not available

Protein Concentration and Protein Localization Data

R1 R2 R3
G1 Pre-START G1 Post-START S/G2 Metaphase Anaphase Telophase G1 Pre-START G1 Post-START S/G2 Metaphase Anaphase Telophase G1 Pre-START G1 Post-START S/G2 Metaphase Anaphase Telophase
Concentration 1.1701 1.37 1.3419 1.304 0.6295 1.2018 4.7393 5.8884 5.3715 5.5065 5.1989 5.2603 2.9119 2.9004 2.827 2.747 2.6317 2.5284
Actin 0.0026 0.0001 0.0065 0 0.0006 0.0021 0.018 0.0002 0.0035 0.0001 0.0027 0.0022 0.0488 0.0001 0.0034 0.0245 0.009 0
Bud 0.0001 0 0.0002 0 0.0001 0 0.0004 0.0001 0.0002 0 0.0001 0.0001 0.0005 0 0.0002 0.0007 0.0007 0
Bud Neck 0.0056 0.0001 0.0004 0 0.0003 0.0007 0.0022 0.0005 0.0005 0.0002 0.0008 0.0007 0.006 0.0001 0.0001 0.0012 0.0016 0.0009
Bud Periphery 0.0002 0 0.0003 0 0.0002 0 0.0008 0.0001 0.0006 0 0.0005 0.0001 0.0014 0 0.0003 0.0008 0.0015 0
Bud Site 0.0002 0 0.0021 0 0.0001 0 0.0011 0.0009 0.0007 0 0.0003 0.0001 0.0018 0.0002 0.0003 0.0083 0.0099 0
Cell Periphery 0.0001 0 0.0001 0 0.0001 0 0.0001 0.0001 0.0004 0 0.0001 0 0.0006 0 0 0.0001 0.0003 0
Cytoplasm 0.0005 0.001 0.0004 0.0001 0.0006 0.0002 0.0037 0.0022 0.0041 0.0034 0.0002 0.0003 0.0017 0.0007 0.0018 0.0016 0.0008 0.0012
Cytoplasmic Foci 0.0054 0 0.0018 0 0.0016 0.0002 0.0053 0.002 0.004 0.001 0.0012 0.0012 0.0197 0 0.0029 0.0087 0.0072 0.0002
Eisosomes 0.0001 0 0.0001 0 0 0 0.0001 0 0.0001 0 0.0001 0 0.0007 0 0 0.0002 0.0001 0
Endoplasmic Reticulum 0.0007 0.0012 0.0005 0 0.0011 0.0002 0.0022 0.0001 0.0042 0.0002 0.0003 0.0004 0.0016 0 0.0014 0.0008 0.0016 0
Endosome 0.0026 0.0005 0.0024 0 0.0108 0.0009 0.0085 0.0018 0.0087 0.0005 0.0029 0.0029 0.0098 0.0001 0.0121 0.0103 0.0064 0
Golgi 0.0009 0 0.0012 0 0.0015 0.0003 0.0027 0.0013 0.0032 0 0.003 0.0011 0.0095 0 0.0047 0.0148 0.0046 0
Lipid Particles 0.0059 0 0.0031 0 0.0164 0.0005 0.0093 0.0093 0.0081 0.0004 0.0221 0.0034 0.0112 0 0.0014 0.0141 0.0171 0
Mitochondria 0.0013 0.0002 0.0021 0.0001 0.0067 0.0002 0.0049 0.0014 0.0103 0.0003 0.034 0.0008 0.0146 0.0006 0.0067 0.0119 0.0177 0.0001
None 0.0006 0.0001 0.0004 0 0.0004 0.0002 0.0024 0.0033 0.001 0.007 0.0002 0.0004 0.0031 0.0018 0.003 0.0019 0.0032 0.0084
Nuclear Periphery 0.0099 0.0201 0.0079 0.001 0.0165 0.0047 0.0165 0.0051 0.0106 0.0047 0.0034 0.003 0.006 0.0022 0.0061 0.0036 0.0063 0.0007
Nucleolus 0.0072 0.0073 0.007 0.0051 0.0284 0.0149 0.009 0.0147 0.0129 0.0095 0.0147 0.0162 0.0095 0.0081 0.005 0.0045 0.0316 0.0128
Nucleus 0.947 0.9672 0.9558 0.9931 0.8974 0.9724 0.899 0.9474 0.9147 0.9381 0.9021 0.9636 0.84 0.9846 0.9473 0.8744 0.8551 0.9725
Peroxisomes 0.0034 0 0.0036 0 0.0013 0.0001 0.0038 0.0017 0.0027 0.0001 0.006 0.0006 0.0071 0 0.0009 0.0072 0.014 0
Punctate Nuclear 0.0048 0.0013 0.0032 0.0006 0.0125 0.0022 0.0054 0.0073 0.005 0.0342 0.0031 0.0023 0.0042 0.0008 0.0008 0.0095 0.0091 0.003
Vacuole 0.0004 0.0006 0.0004 0 0.0016 0.0001 0.0026 0.0005 0.0028 0.0001 0.001 0.0003 0.0012 0.0004 0.0009 0.0005 0.0016 0.0001
Vacuole Periphery 0.0004 0.0004 0.0004 0 0.0019 0.0001 0.0018 0.0001 0.0018 0.0001 0.0013 0.0003 0.0008 0.0001 0.0006 0.0005 0.0006 0

Sequencing Data

R1 R2
G1 Post-START S/G2 Metaphase Anaphase Telophase G1 Post-START S/G2 Metaphase Anaphase Telophase
Gene Expression 17.2192 4.9257 5.4826 5.8167 13.3952 9.6374 5.6029 7.9834 7.6765 11.132
Translational Efficiency 1.3793 2.4269 2.0848 1.8383 1.5652 2.6202 1.9829 1.5116 1.4374 1.5819

Hit Data

Dataset Hit
Protein Concentration ✘
Protein Localization ✘
Gene Expression ✘
Translational Efficiency ✘
Standard name
Human Ortholog
Description Subunit of the RES complex; RES complex is required for nuclear pre-mRNA retention and splicing; involved in bud-site selection; diploid mutants display a unipolar budding pattern instead of the wild-type bipolar pattern due to a specific defect in MATa1 pre-mRNA splicing which leads to haploid gene expression in diploids
Localization
Cell Percentages nucleus (99%)
Cell Cycle Regulation No
Subcompartmental Group nucleus-4

Bud13

Bud13


Nearest neighbours ( genes)

correlation threshold

0.6

1.0

0.80


Biological Process (Gene Ontology)
Molecular Function (Gene Ontology)
Cellular Component (Gene Ontology)

Endocytosis
Temp Actin Patch (Sac6-tdTomato) Cortical Patch (Sla1-GFP) Late Endosome (Snf7-GFP) Vacuole (Vph1-GFP)
37℃
RT
Cell Cycle Omics

CYCLoPs (Bud13-GFP)
Gene / Allele Actin Patch (Sac6-tdTomato) Cortical Patch (Sla1-GFP) Late Endosome (Snf7-GFP) Vacuole (Sac6-tdTomato)
Gene Images
Gene Images
Images are not yet available
Images are not yet available