Standard name
Description FG-nucleoporin component of central core of the nuclear pore complex; contributes directly to nucleocytoplasmic transport and maintenance of the nuclear pore complex (NPC) permeability barrier; found in stable complex with Nic96p and two other FG-nucleoproteins (Nsp1p and Nup57p)

Micrographs

ensLOC DeepLoc
Localization WT1 WT2 WT3 RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3 WT1 WT2 WT3 AF100 AF140 AF180
Cortical Patches 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Bud 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Bud Neck 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Bud Site 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Cell Periphery 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Cytoplasm 0 0 0 0 0 0 0 0 0 0 0 0 0.08 0.12 0.07 0.06 0.13 0 0 0 0 0 0
Endoplasmic Reticulum 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Endosome 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0.05 0 0 0 0 0 0 0
Golgi 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Mitochondria 0 0.1 0.19 0.18 0.12 0.25 0.22 0.24 0.45 0.41 0.38 0 0 0 0.08 0.11 0.05 0 0 0 0 0.06 0.07
Nucleus 0 0.08 0 0.05 0 0 0.05 0.07 0.07 0.1 0.05 0.09 0 0 0.06 0.05 0 0 0 0 0 0 0
Nuclear Periphery 0.88 0.79 0.81 0.82 0.79 0.68 0.67 0.56 0.5 0.5 0.57 0.85 0.81 0.72 0.6 0.53 0.45 0.9 0.8 0.89 0.86 0.84 0.74
Nucleolus 0.21 0.1 0.2 0.21 0.3 0.31 0.32 0.43 0.43 0.39 0.44 0.11 0.08 0.06 0.19 0.25 0.25 0 0 0 0 0 0
Peroxisomes 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
SpindlePole 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Vac/Vac Membrane 0.13 0.14 0.07 0.07 0.09 0.05 0 0.05 0 0.06 0 0.14 0.15 0.26 0.29 0.28 0.32 0 0.06 0 0 0 0.06
ensLOC DeepLoc
Localization WT1 WT2 WT3 RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3 WT1 WT2 WT3 AF100 AF140 AF180
Cortical Patches 0 0 1 1 0 0 0 0 1 0 0 0 1 1 0 0 0 0 0 1 1 4 2
Bud 0 0 0 0 0 0 1 1 2 1 0 0 0 0 0 0 0 0 1 3 0 1 4
Bud Neck 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Bud Site 0 0 0 1 1 2 0 2 1 6 1 0 1 0 1 0 0
Cell Periphery 0 0 0 0 0 0 0 0 0 1 0 0 0 0 0 0 0 0 0 0 0 0 0
Cytoplasm 2 2 7 9 11 7 14 13 4 11 4 6 12 30 12 11 14 0 0 2 0 0 1
Endoplasmic Reticulum 0 0 1 3 5 2 3 0 0 2 2 0 0 1 2 3 2 0 0 0 0 0 1
Endosome 0 0 6 2 6 5 8 5 1 0 4 2 2 3 7 9 3 0 2 5 2 8 9
Golgi 0 0 0 0 0 0 0 0 0 0 0 0 0 0 1 0 0 0 1 1 0 3 7
Mitochondria 3 15 79 60 37 93 77 134 156 149 140 4 1 1 13 19 6 1 3 6 6 21 26
Nucleus 3 12 10 16 7 16 17 38 24 35 20 19 6 10 10 9 4 1 4 3 2 0 7
Nuclear Periphery 84 122 335 269 241 251 234 311 175 183 211 181 128 182 101 92 50 94 145 414 143 336 277
Nucleolus 20 16 83 70 91 116 112 236 149 140 165 24 12 14 32 43 27 0 7 6 2 6 7
Peroxisomes 0 1 1 1 1 0 1 2 0 0 1 0 1 0 0 1 0 0 0 0 0 1 2
SpindlePole 0 0 0 0 1 1 0 3 2 1 0 0 2 9 1 1 0 0 0 4 0 2 3
Vac/Vac Membrane 12 21 28 24 27 18 15 29 5 20 14 30 24 67 49 49 35 4 10 11 3 8 21
Unique Cell Count 95 155 414 330 305 369 348 555 349 363 373 214 159 254 169 174 110 105 182 465 166 398 376
Labelled Cell Count 124 189 551 456 428 511 482 774 520 549 562 266 190 318 229 237 141 105 182 465 166 398 376


Nuclear Periphery

(Huh et al. Nature 2003 425:686)
Screen WT1 WT2 WT3 RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3 AF100 AF140 AF180
Mean Cell GFP Intensity (1e-4) 11.1 13.1 11.3 11.1 11.5 10.0 10.0 9.9 9.0 8.8 9.7 11.8 12.5 13.2 13.5 13.9 17.9 12.8 13.2 13.7
Std Deviation (1e-4) 2.4 3.5 3.3 4.0 3.4 3.3 3.5 3.4 3.9 3.9 3.1 3.1 3.5 4.6 5.2 6.0 7.2 3.7 2.8 3.3
Intensity Change (Log2) -0.02 0.02 -0.18 -0.17 -0.19 -0.32 -0.35 -0.22 0.07 0.14 0.22 0.26 0.3 0.67 0.18 0.22 0.28


Localization RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3
Cortical Patches 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Bud 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Bud Neck 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Bud Site 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Cell Periphery 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Cytoplasm 1.0 1.6 0.2 2.0 0.7 -0.6 1.2 -0.7 0 3.5 5.6 3.3 3.0 0
Endoplasmic Reticulum 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Endosome 0 0.5 -0.1 0.9 0 0 0 0 0 0 0 0 0 0
Golgi 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Mitochondria -0.3 -2.5 2.1 1.0 1.9 7.6 6.7 5.8 -6.0 -5.7 -7.2 -3.4 -2.4 -3.4
Nucleus 1.8 -0.1 1.5 1.8 3.1 3.0 4.3 2.2 3.7 0 1.1 2.1 1.7 0
Nuclear Periphery 0.2 -0.6 -4.2 -4.3 -8.1 -9.0 -9.0 -7.4 1.1 -0.1 -2.8 -5.3 -7.0 -7.5
Nucleolus 0.4 3.0 3.7 3.8 7.4 6.8 5.7 7.3 -2.8 -3.6 -5.2 -0.3 1.3 1.0
Peroxisomes 0 0 0 0 0 0 0 0 0 0 0 0 0 0
SpindlePole 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Vacuole 0.3 1.0 -1.1 -1.5 -1.0 -3.6 -0.7 -1.9 3.0 3.1 7.0 7.2 7.0 7.2


Endocytosis
Temp Actin Patch (Sac6-tdTomato) Cortical Patch (Sla1-GFP) Late Endosome (Snf7-GFP) Vacuole (Vph1-GFP)
37℃
RT
Cell Cycle Omics

CYCLoPs (Nup49-GFP)
Gene / Allele Actin Patch (Sac6-tdTomato) Cortical Patch (Sla1-GFP) Late Endosome (Snf7-GFP) Vacuole (Sac6-tdTomato)
Gene Images
Gene Images
Images are not yet available
Images are not yet available