| Standard name | |
|---|---|
| Description | Protein that interacts with Rab GTPases; localized to late Golgi vesicles; computational analysis of large-scale protein-protein interaction data suggests a possible role in vesicle-mediated transport |
Micrographs
Sub-cellular Localization
Yeast GFP Assignment
Protein Abundance
Localization Change
External localization resources
| ensLOC | DeepLoc | |||||||||||||||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|
| Localization | WT1 | WT2 | WT3 | RAP60 | RAP140 | RAP220 | RAP300 | RAP380 | RAP460 | RAP540 | RAP620 | RAP700 | HU80 | HU120 | HU160 | rpd3Δ_1 | rpd3Δ_2 | rpd3Δ_3 | WT1 | WT2 | WT3 | AF100 | AF140 | AF180 |
| Cortical Patches | 1 | 0 | 0 | 0 | 0 | 0 | 0 | 1 | 1 | 0 | 1 | 3 | 0 | 0 | 0 | 7 | 6 | 7 | 7 | 1 | 0 | 1 | 4 | 7 |
| Bud | 2 | 0 | 0 | 1 | 0 | 1 | 9 | 8 | 7 | 5 | 12 | 9 | 0 | 1 | 0 | 1 | 5 | 5 | 0 | 0 | 0 | 0 | 6 | 2 |
| Bud Neck | 0 | 0 | 0 | 2 | 3 | 1 | 3 | 13 | 8 | 10 | 1 | 3 | 3 | 1 | 4 | 4 | 2 | 1 | 2 | 2 | 0 | 0 | 2 | 6 |
| Bud Site | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 1 | 1 | 1 | 1 | 1 | 0 | 0 | 0 | 0 | 0 | 0 | – | – | – | – | – | – |
| Cell Periphery | 0 | 0 | 0 | 1 | 3 | 4 | 1 | 1 | 1 | 1 | 1 | 0 | 1 | 3 | 3 | 10 | 7 | 10 | 0 | 0 | 0 | 2 | 6 | 7 |
| Cytoplasm | 179 | 102 | 16 | 160 | 261 | 238 | 382 | 353 | 196 | 311 | 215 | 218 | 266 | 370 | 463 | 277 | 271 | 227 | 117 | 86 | 36 | 150 | 251 | 307 |
| Endoplasmic Reticulum | 8 | 3 | 0 | 1 | 2 | 0 | 0 | 0 | 1 | 1 | 4 | 11 | 1 | 0 | 1 | 18 | 16 | 10 | 31 | 3 | 2 | 2 | 5 | 7 |
| Endosome | 0 | 1 | 0 | 0 | 1 | 0 | 2 | 1 | 1 | 5 | 1 | 0 | 0 | 1 | 0 | 6 | 8 | 10 | 6 | 2 | 0 | 1 | 4 | 7 |
| Golgi | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 1 | 2 | 4 | 0 | 1 | 0 | 0 | 1 | 1 |
| Mitochondria | 32 | 3 | 1 | 4 | 0 | 25 | 78 | 49 | 96 | 89 | 169 | 101 | 1 | 1 | 2 | 4 | 5 | 5 | 53 | 13 | 5 | 23 | 29 | 40 |
| Nucleus | 0 | 0 | 0 | 2 | 2 | 1 | 1 | 2 | 1 | 0 | 2 | 1 | 0 | 0 | 0 | 0 | 2 | 0 | 0 | 0 | 0 | 0 | 1 | 0 |
| Nuclear Periphery | 0 | 1 | 0 | 0 | 0 | 0 | 2 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 1 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
| Nucleolus | 0 | 0 | 0 | 1 | 0 | 0 | 0 | 0 | 0 | 1 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
| Peroxisomes | 0 | 0 | 0 | 0 | 0 | 1 | 2 | 0 | 0 | 1 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
| SpindlePole | 0 | 0 | 0 | 0 | 1 | 2 | 4 | 7 | 2 | 7 | 4 | 3 | 0 | 0 | 0 | 1 | 2 | 2 | 1 | 0 | 0 | 1 | 3 | 6 |
| Vac/Vac Membrane | 2 | 1 | 0 | 3 | 1 | 4 | 2 | 2 | 3 | 8 | 3 | 5 | 0 | 1 | 3 | 6 | 8 | 6 | 11 | 7 | 2 | 3 | 7 | 13 |
| Unique Cell Count | 208 | 110 | 16 | 167 | 266 | 251 | 436 | 397 | 261 | 386 | 323 | 316 | 267 | 373 | 468 | 306 | 298 | 255 | 238 | 122 | 51 | 194 | 335 | 420 |
| Labelled Cell Count | 224 | 111 | 17 | 175 | 274 | 277 | 486 | 438 | 318 | 440 | 414 | 355 | 272 | 378 | 476 | 336 | 334 | 287 | 238 | 122 | 51 | 194 | 335 | 420 |
Yeast GFP Assignment
Protein Abundance
| Screen | WT1 | WT2 | WT3 | RAP60 | RAP140 | RAP220 | RAP300 | RAP380 | RAP460 | RAP540 | RAP620 | RAP700 | HU80 | HU120 | HU160 | rpd3Δ_1 | rpd3Δ_2 | rpd3Δ_3 | AF100 | AF140 | AF180 |
|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|
| Mean Cell GFP Intensity (1e-4) | 3.5 | 4.4 | 3.9 | 4.6 | 5.0 | 4.3 | 3.9 | 4.2 | 3.8 | 3.8 | 3.5 | 3.7 | 5.2 | 5.2 | 5.1 | 5.9 | 6.6 | 6.6 | 4.5 | 5.0 | 5.1 |
| Std Deviation (1e-4) | 0.4 | 0.7 | 0.6 | 1.3 | 1.3 | 1.3 | 1.2 | 0.9 | 1.6 | 1.1 | 1.0 | 0.7 | 1.2 | 0.9 | 1.4 | 1.0 | 1.6 | 1.8 | 0.7 | 1.6 | 1.4 |
| Intensity Change (Log2) | – | – | – | 0.21 | 0.34 | 0.13 | -0.01 | 0.08 | -0.06 | -0.05 | -0.19 | -0.1 | 0.39 | 0.4 | 0.38 | 0.59 | 0.73 | 0.74 | 0.18 | 0.33 | 0.38 |
Localization Change
| Localization | RAP60 | RAP140 | RAP220 | RAP300 | RAP380 | RAP460 | RAP540 | RAP620 | RAP700 | HU80 | HU120 | HU160 | rpd3Δ_1 | rpd3Δ_2 | rpd3Δ_3 |
|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|
| Actin | – | – | – | – | – | – | – | – | – | – | – | – | – | – | – |
| Bud | – | – | – | – | – | – | – | – | – | – | – | – | – | – | – |
| Bud Neck | – | – | – | – | – | – | – | – | – | – | – | – | – | – | – |
| Bud Site | – | – | – | – | – | – | – | – | – | – | – | – | – | – | – |
| Cell Periphery | – | – | – | – | – | – | – | – | – | – | – | – | – | – | – |
| Cyto | – | – | – | – | – | – | – | – | – | – | – | – | – | – | – |
| Endoplasmic Reticulum | – | – | – | – | – | – | – | – | – | – | – | – | – | – | – |
| Endosome | – | – | – | – | – | – | – | – | – | – | – | – | – | – | – |
| Golgi | – | – | – | – | – | – | – | – | – | – | – | – | – | – | – |
| Mitochondria | – | – | – | – | – | – | – | – | – | – | – | – | – | – | – |
| Nuclear Periphery | – | – | – | – | – | – | – | – | – | – | – | – | – | – | – |
| Nuc | – | – | – | – | – | – | – | – | – | – | – | – | – | – | – |
| Nucleolus | – | – | – | – | – | – | – | – | – | – | – | – | – | – | – |
| Peroxisomes | – | – | – | – | – | – | – | – | – | – | – | – | – | – | – |
| SpindlePole | – | – | – | – | – | – | – | – | – | – | – | – | – | – | – |
| Vac | – | – | – | – | – | – | – | – | – | – | – | – | – | – | – |
| Cortical Patches | – | – | – | – | – | – | – | – | – | – | – | – | – | – | – |
| Cytoplasm | – | – | – | – | – | – | – | – | – | – | – | – | – | – | – |
| Nucleus | – | – | – | – | – | – | – | – | – | – | – | – | – | – | – |
| Vacuole | – | – | – | – | – | – | – | – | – | – | – | – | – | – | – |
External localization resources
