Standard name
Description NADPH-dependent aldehyde reductase; utilizes aromatic and alophatic aldehyde substrates; member of the short-chain dehydrogenase/reductase superfamily
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Micrographs

ensLOC DeepLoc
Localization WT1 WT2 WT3 RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3 WT1 WT2 WT3 AF100 AF140 AF180
Cortical Patches – – 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 – 0 0 0 0 0
Bud – – 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 – 0 0 0 0 0
Bud Neck – – 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 – 0 0 0 0 0
Bud Site – – 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 – – – – – –
Cell Periphery – – 0 0 0.05 0 0 0 0 0 0 0.06 0.09 0 0 0.09 0.06 0.07 – 0 0 0 0 0
Cytoplasm – – 0.9 0.82 0.84 0.79 0.73 0.71 0.64 0.65 0.57 0.63 0.98 0.95 0.95 0.93 0.87 0.92 – 0.88 0.93 0.93 0.85 0.84
Endoplasmic Reticulum – – 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 – 0 0 0 0 0
Endosome – – 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 – 0 0 0 0 0
Golgi – – 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 – 0 0 0 0 0
Mitochondria – – 0 0 0 0.16 0.23 0.24 0.36 0.26 0.49 0.25 0 0 0 0 0 0 – 0 0 0 0.05 0
Nucleus – – 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 – 0 0 0 0 0
Nuclear Periphery – – 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 – 0 0 0 0 0
Nucleolus – – 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 – 0 0 0 0 0
Peroxisomes – – 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 – 0 0 0 0 0
SpindlePole – – 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 – 0 0 0 0 0
Vac/Vac Membrane – – 0.07 0.15 0.13 0.1 0.14 0.09 0.07 0.13 0.15 0.14 0 0.07 0.07 0.06 0.14 0.07 – 0 0 0 0 0
ensLOC DeepLoc
Localization WT1 WT2 WT3 RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3 WT1 WT2 WT3 AF100 AF140 AF180
Cortical Patches – – 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 – 0 0 0 0 1
Bud – – 0 0 0 1 2 3 0 4 2 1 0 0 0 0 0 0 – 1 0 0 0 0
Bud Neck – – 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 – 0 0 0 0 0
Bud Site – – 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 – – – – – –
Cell Periphery – – 1 1 7 2 4 5 3 3 5 10 4 1 3 46 35 37 – 0 0 0 0 1
Cytoplasm – – 27 78 123 115 145 114 110 130 95 112 43 87 77 497 489 488 – 29 61 52 102 115
Endoplasmic Reticulum – – 0 1 0 1 0 0 3 1 0 3 0 0 0 14 15 13 – 0 0 0 1 2
Endosome – – 0 0 0 0 0 0 0 1 0 0 0 0 0 2 0 1 – 0 0 0 1 0
Golgi – – 0 0 0 0 1 0 0 0 0 0 0 0 0 2 3 2 – 0 0 0 1 2
Mitochondria – – 0 3 6 23 47 38 62 51 82 44 0 0 0 2 6 5 – 0 0 1 6 5
Nucleus – – 1 0 0 3 4 7 5 7 6 6 0 1 0 4 0 6 – 0 0 0 0 0
Nuclear Periphery – – 0 0 0 0 0 0 2 0 1 1 0 0 0 0 1 0 – 0 0 0 0 0
Nucleolus – – 0 1 0 0 0 0 1 0 1 0 0 0 0 0 0 0 – 0 0 0 0 0
Peroxisomes – – 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 – 0 0 0 0 0
SpindlePole – – 0 0 0 0 0 1 0 0 0 0 0 0 0 0 0 0 – 0 0 0 0 0
Vac/Vac Membrane – – 2 14 19 15 28 15 12 26 25 24 0 6 6 30 80 36 – 0 0 0 1 2
Unique Cell Count 30 95 146 146 200 160 172 199 166 177 44 92 81 536 559 532 34 66 57 121 138
Labelled Cell Count 31 98 155 160 231 183 198 223 217 201 47 95 86 597 629 588 34 66 57 121 138


Cytoplasm, Nucleus

(Huh et al. Nature 2003 425:686)
Screen WT1 WT2 WT3 RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3 AF100 AF140 AF180
Mean Cell GFP Intensity (1e-4) – – 8.0 7.4 7.9 6.5 6.4 6.8 5.6 6.3 5.8 6.4 8.8 9.8 9.8 8.7 8.3 8.2 8.8 8.1 8.8
Std Deviation (1e-4) – – 1.1 1.7 2.4 1.6 1.6 2.0 1.4 1.6 1.5 1.5 1.3 1.9 2.0 1.1 1.3 1.2 3.1 1.7 2.6
Intensity Change (Log2) – – – -0.1 -0.02 -0.3 -0.32 -0.24 -0.52 -0.34 -0.45 -0.31 0.14 0.29 0.29 0.12 0.06 0.04 0.14 0.02 0.13


Localization RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3
Cortical Patches 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Bud 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Bud Neck 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Bud Site 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Cell Periphery 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Cytoplasm -1.0 -0.8 -1.4 -2.1 -2.2 -2.8 -2.7 -3.4 -2.9 1.4 0.9 1.0 0.6 -0.4 0.3
Endoplasmic Reticulum 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Endosome 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Golgi 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Mitochondria 0 0 0 3.0 3.0 4.0 3.1 5.0 3.1 0 0 0 0 0 0
Nucleus 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Nuclear Periphery 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Nucleolus 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Peroxisomes 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
SpindlePole 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Vacuole 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0


Endocytosis
Temp Actin Patch (Sac6-tdTomato) Cortical Patch (Sla1-GFP) Late Endosome (Snf7-GFP) Vacuole (Vph1-GFP)
37℃
RT
Cell Cycle Omics

CYCLoPs (Ari1-GFP)
Gene / Allele Actin Patch (Sac6-tdTomato) Cortical Patch (Sla1-GFP) Late Endosome (Snf7-GFP) Vacuole (Sac6-tdTomato)
Gene Images
Gene Images
Images are not yet available
Images are not yet available