Standard name
Human Ortholog
Description NADPH-dependent aldehyde reductase; utilizes aromatic and alophatic aldehyde substrates; member of the short-chain dehydrogenase/reductase superfamily
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Micrographs

ensLOC DeepLoc
Localization WT1 WT2 WT3 RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3 WT1 WT2 WT3 AF100 AF140 AF180
Cortical Patches – – 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 – 0 0 0 0 0
Bud – – 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 – 0 0 0 0 0
Bud Neck – – 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 – 0 0 0 0 0
Bud Site – – 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 – – – – – –
Cell Periphery – – 0 0 0.05 0 0 0 0 0 0 0.06 0.09 0 0 0.09 0.06 0.07 – 0 0 0 0 0
Cytoplasm – – 0.9 0.82 0.84 0.79 0.73 0.71 0.64 0.65 0.57 0.63 0.98 0.95 0.95 0.93 0.87 0.92 – 0.88 0.93 0.93 0.85 0.84
Endoplasmic Reticulum – – 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 – 0 0 0 0 0
Endosome – – 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 – 0 0 0 0 0
Golgi – – 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 – 0 0 0 0 0
Mitochondria – – 0 0 0 0.16 0.23 0.24 0.36 0.26 0.49 0.25 0 0 0 0 0 0 – 0 0 0 0.05 0
Nucleus – – 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 – 0 0 0 0 0
Nuclear Periphery – – 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 – 0 0 0 0 0
Nucleolus – – 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 – 0 0 0 0 0
Peroxisomes – – 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 – 0 0 0 0 0
SpindlePole – – 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 – 0 0 0 0 0
Vac/Vac Membrane – – 0.07 0.15 0.13 0.1 0.14 0.09 0.07 0.13 0.15 0.14 0 0.07 0.07 0.06 0.14 0.07 – 0 0 0 0 0
ensLOC DeepLoc
Localization WT1 WT2 WT3 RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3 WT1 WT2 WT3 AF100 AF140 AF180
Cortical Patches – – 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 – 0 0 0 0 1
Bud – – 0 0 0 1 2 3 0 4 2 1 0 0 0 0 0 0 – 1 0 0 0 0
Bud Neck – – 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 – 0 0 0 0 0
Bud Site – – 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 – – – – – –
Cell Periphery – – 1 1 7 2 4 5 3 3 5 10 4 1 3 46 35 37 – 0 0 0 0 1
Cytoplasm – – 27 78 123 115 145 114 110 130 95 112 43 87 77 497 489 488 – 29 61 52 102 115
Endoplasmic Reticulum – – 0 1 0 1 0 0 3 1 0 3 0 0 0 14 15 13 – 0 0 0 1 2
Endosome – – 0 0 0 0 0 0 0 1 0 0 0 0 0 2 0 1 – 0 0 0 1 0
Golgi – – 0 0 0 0 1 0 0 0 0 0 0 0 0 2 3 2 – 0 0 0 1 2
Mitochondria – – 0 3 6 23 47 38 62 51 82 44 0 0 0 2 6 5 – 0 0 1 6 5
Nucleus – – 1 0 0 3 4 7 5 7 6 6 0 1 0 4 0 6 – 0 0 0 0 0
Nuclear Periphery – – 0 0 0 0 0 0 2 0 1 1 0 0 0 0 1 0 – 0 0 0 0 0
Nucleolus – – 0 1 0 0 0 0 1 0 1 0 0 0 0 0 0 0 – 0 0 0 0 0
Peroxisomes – – 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 – 0 0 0 0 0
SpindlePole – – 0 0 0 0 0 1 0 0 0 0 0 0 0 0 0 0 – 0 0 0 0 0
Vac/Vac Membrane – – 2 14 19 15 28 15 12 26 25 24 0 6 6 30 80 36 – 0 0 0 1 2
Unique Cell Count 30 95 146 146 200 160 172 199 166 177 44 92 81 536 559 532 34 66 57 121 138
Labelled Cell Count 31 98 155 160 231 183 198 223 217 201 47 95 86 597 629 588 34 66 57 121 138


Cytoplasm, Nucleus

(Huh et al. Nature 2003 425:686)
Screen WT1 WT2 WT3 RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3 AF100 AF140 AF180
Mean Cell GFP Intensity (1e-4) – – 8.0 7.4 7.9 6.5 6.4 6.8 5.6 6.3 5.8 6.4 8.8 9.8 9.8 8.7 8.3 8.2 8.8 8.1 8.8
Std Deviation (1e-4) – – 1.1 1.7 2.4 1.6 1.6 2.0 1.4 1.6 1.5 1.5 1.3 1.9 2.0 1.1 1.3 1.2 3.1 1.7 2.6
Intensity Change (Log2) – – – -0.1 -0.02 -0.3 -0.32 -0.24 -0.52 -0.34 -0.45 -0.31 0.14 0.29 0.29 0.12 0.06 0.04 0.14 0.02 0.13


Localization RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3
Cortical Patches 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Bud 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Bud Neck 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Bud Site 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Cell Periphery 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Cytoplasm -1.0 -0.8 -1.4 -2.1 -2.2 -2.8 -2.7 -3.4 -2.9 1.4 0.9 1.0 0.6 -0.4 0.3
Endoplasmic Reticulum 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Endosome 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Golgi 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Mitochondria 0 0 0 3.0 3.0 4.0 3.1 5.0 3.1 0 0 0 0 0 0
Nucleus 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Nuclear Periphery 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Nucleolus 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Peroxisomes 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
SpindlePole 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Vacuole 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0

Images

Circle plot not available

Protein Concentration and Protein Localization Data

R1 R2 R3
G1 Pre-START G1 Post-START S/G2 Metaphase Anaphase Telophase G1 Pre-START G1 Post-START S/G2 Metaphase Anaphase Telophase G1 Pre-START G1 Post-START S/G2 Metaphase Anaphase Telophase
Concentration – – – – – – – – – – – – – – – – – –
Actin 0.5112 0.0189 0.0033 0.0003 0.0001 0.001 0.1643 0.0013 0.0003 0.0004 0.0003 0.0002 – – – – – –
Bud 0.003 0.0003 0.0003 0.0001 0.0001 0.0002 0.0023 0.0004 0.0003 0.0003 0.0004 0.0003 – – – – – –
Bud Neck 0.2775 0.0002 0.0004 0.0008 0.0002 0.0011 0.009 0.0004 0.0007 0.0013 0.0018 0.002 – – – – – –
Bud Periphery 0.0038 0.0001 0 0 0 0 0.0022 0.0002 0.0001 0.0001 0.0001 0.0001 – – – – – –
Bud Site 0.026 0.0013 0.0003 0 0 0.0001 0.0051 0.0014 0.0005 0.0003 0.0005 0.0002 – – – – – –
Cell Periphery 0.0029 0.0001 0 0 0 0 0.0006 0 0 0 0 0 – – – – – –
Cytoplasm 0.0049 0.9495 0.9073 0.9859 0.994 0.9146 0.4416 0.9446 0.8926 0.88 0.8875 0.8711 – – – – – –
Cytoplasmic Foci 0.0195 0.0035 0.0106 0.0018 0.0032 0.0065 0.0517 0.0056 0.0069 0.0064 0.0044 0.0066 – – – – – –
Eisosomes 0.0036 0.0002 0 0 0 0 0.0019 0 0 0 0 0 – – – – – –
Endoplasmic Reticulum 0.0115 0.0016 0.0016 0.0006 0.0001 0.0032 0.0196 0.0013 0.002 0.0023 0.002 0.0024 – – – – – –
Endosome 0.0288 0.0012 0.0158 0.0003 0.0011 0.0071 0.125 0.002 0.005 0.0049 0.0034 0.0081 – – – – – –
Golgi 0.0519 0.003 0.0458 0 0.0001 0.0005 0.0229 0.0002 0.0002 0.0001 0.0001 0.0002 – – – – – –
Lipid Particles 0.0181 0.0001 0.0001 0 0 0.0001 0.0184 0.0003 0 0 0 0 – – – – – –
Mitochondria 0.013 0.0018 0.001 0.0001 0.0001 0.0004 0.0131 0.0011 0.0004 0.0004 0.0003 0.001 – – – – – –
None 0.0013 0.0104 0.0029 0.0028 0.0007 0.0067 0.0408 0.0086 0.006 0.0068 0.0055 0.0061 – – – – – –
Nuclear Periphery 0.0028 0.0011 0.001 0.0007 0 0.003 0.0308 0.0021 0.0048 0.0069 0.0042 0.0051 – – – – – –
Nucleolus 0.0007 0 0 0 0 0 0.002 0.0001 0.0001 0.0001 0.0001 0.0001 – – – – – –
Nucleus 0.0008 0.0053 0.0087 0.0055 0.0001 0.0529 0.0155 0.026 0.0751 0.0825 0.084 0.0902 – – – – – –
Peroxisomes 0.0141 0.0003 0.0001 0 0 0.0001 0.0096 0.001 0.0002 0.0002 0.0001 0.0001 – – – – – –
Punctate Nuclear 0.0017 0.0005 0.0005 0.0007 0 0.0007 0.0141 0.0028 0.0031 0.0049 0.0037 0.0033 – – – – – –
Vacuole 0.0018 0.0004 0.0003 0.0002 0.0001 0.0015 0.0054 0.0005 0.0014 0.0016 0.0016 0.0024 – – – – – –
Vacuole Periphery 0.001 0.0001 0.0001 0 0 0.0003 0.0043 0.0001 0.0003 0.0004 0.0004 0.0006 – – – – – –

Sequencing Data

R1 R2
G1 Post-START S/G2 Metaphase Anaphase Telophase G1 Post-START S/G2 Metaphase Anaphase Telophase
Gene Expression 70.2839 54.7987 25.2391 37.0792 25.3509 64.4353 43.4657 42.2316 49.6423 48.0426
Translational Efficiency 1.6882 1.1037 1.3661 1.2309 1.5055 2.0887 1.3536 1.2446 1.2706 1.1006

Hit Data

Dataset Hit
Protein Concentration –
Protein Localization ✘
Gene Expression ✘
Translational Efficiency ✘
Standard name
Human Ortholog
Description NADPH-dependent aldehyde reductase; utilizes aromatic and alophatic aldehyde substrates; member of the short-chain dehydrogenase/reductase superfamily
Localization
Cell Percentages cytoplasm (86%)
Cell Cycle Regulation No
Subcompartmental Group cytoplasm-9

Ari1

Ari1


Nearest neighbours ( genes)

correlation threshold

0.6

1.0

0.80


Biological Process (Gene Ontology)
Molecular Function (Gene Ontology)
Cellular Component (Gene Ontology)

Endocytosis
Temp Actin Patch (Sac6-tdTomato) Cortical Patch (Sla1-GFP) Late Endosome (Snf7-GFP) Vacuole (Vph1-GFP)
37℃
RT
Cell Cycle Omics

CYCLoPs (Ari1-GFP)
Gene / Allele Actin Patch (Sac6-tdTomato) Cortical Patch (Sla1-GFP) Late Endosome (Snf7-GFP) Vacuole (Sac6-tdTomato)
Gene Images
Gene Images
Images are not yet available
Images are not yet available