Standard name | |
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Description | NADPH-dependent aldehyde reductase; utilizes aromatic and alophatic aldehyde substrates; member of the short-chain dehydrogenase/reductase superfamily |
ensLOC | DeepLoc | |||||||||||||||||||||||
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Localization | WT1 | WT2 | WT3 | RAP60 | RAP140 | RAP220 | RAP300 | RAP380 | RAP460 | RAP540 | RAP620 | RAP700 | HU80 | HU120 | HU160 | rpd3Δ_1 | rpd3Δ_2 | rpd3Δ_3 | WT1 | WT2 | WT3 | AF100 | AF140 | AF180 |
Cortical Patches | – | – | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | – | 0 | 0 | 0 | 0 | 1 |
Bud | – | – | 0 | 0 | 0 | 1 | 2 | 3 | 0 | 4 | 2 | 1 | 0 | 0 | 0 | 0 | 0 | 0 | – | 1 | 0 | 0 | 0 | 0 |
Bud Neck | – | – | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | – | 0 | 0 | 0 | 0 | 0 |
Bud Site | – | – | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | – | – | – | – | – | – |
Cell Periphery | – | – | 1 | 1 | 7 | 2 | 4 | 5 | 3 | 3 | 5 | 10 | 4 | 1 | 3 | 46 | 35 | 37 | – | 0 | 0 | 0 | 0 | 1 |
Cytoplasm | – | – | 27 | 78 | 123 | 115 | 145 | 114 | 110 | 130 | 95 | 112 | 43 | 87 | 77 | 497 | 489 | 488 | – | 29 | 61 | 52 | 102 | 115 |
Endoplasmic Reticulum | – | – | 0 | 1 | 0 | 1 | 0 | 0 | 3 | 1 | 0 | 3 | 0 | 0 | 0 | 14 | 15 | 13 | – | 0 | 0 | 0 | 1 | 2 |
Endosome | – | – | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 1 | 0 | 0 | 0 | 0 | 0 | 2 | 0 | 1 | – | 0 | 0 | 0 | 1 | 0 |
Golgi | – | – | 0 | 0 | 0 | 0 | 1 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 2 | 3 | 2 | – | 0 | 0 | 0 | 1 | 2 |
Mitochondria | – | – | 0 | 3 | 6 | 23 | 47 | 38 | 62 | 51 | 82 | 44 | 0 | 0 | 0 | 2 | 6 | 5 | – | 0 | 0 | 1 | 6 | 5 |
Nucleus | – | – | 1 | 0 | 0 | 3 | 4 | 7 | 5 | 7 | 6 | 6 | 0 | 1 | 0 | 4 | 0 | 6 | – | 0 | 0 | 0 | 0 | 0 |
Nuclear Periphery | – | – | 0 | 0 | 0 | 0 | 0 | 0 | 2 | 0 | 1 | 1 | 0 | 0 | 0 | 0 | 1 | 0 | – | 0 | 0 | 0 | 0 | 0 |
Nucleolus | – | – | 0 | 1 | 0 | 0 | 0 | 0 | 1 | 0 | 1 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | – | 0 | 0 | 0 | 0 | 0 |
Peroxisomes | – | – | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | – | 0 | 0 | 0 | 0 | 0 |
SpindlePole | – | – | 0 | 0 | 0 | 0 | 0 | 1 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | – | 0 | 0 | 0 | 0 | 0 |
Vac/Vac Membrane | – | – | 2 | 14 | 19 | 15 | 28 | 15 | 12 | 26 | 25 | 24 | 0 | 6 | 6 | 30 | 80 | 36 | – | 0 | 0 | 0 | 1 | 2 |
Unique Cell Count | 30 | 95 | 146 | 146 | 200 | 160 | 172 | 199 | 166 | 177 | 44 | 92 | 81 | 536 | 559 | 532 | 34 | 66 | 57 | 121 | 138 | |||
Labelled Cell Count | 31 | 98 | 155 | 160 | 231 | 183 | 198 | 223 | 217 | 201 | 47 | 95 | 86 | 597 | 629 | 588 | 34 | 66 | 57 | 121 | 138 |
Screen | WT1 | WT2 | WT3 | RAP60 | RAP140 | RAP220 | RAP300 | RAP380 | RAP460 | RAP540 | RAP620 | RAP700 | HU80 | HU120 | HU160 | rpd3Δ_1 | rpd3Δ_2 | rpd3Δ_3 | AF100 | AF140 | AF180 |
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Mean Cell GFP Intensity (1e-4) | – | – | 8.0 | 7.4 | 7.9 | 6.5 | 6.4 | 6.8 | 5.6 | 6.3 | 5.8 | 6.4 | 8.8 | 9.8 | 9.8 | 8.7 | 8.3 | 8.2 | 8.8 | 8.1 | 8.8 |
Std Deviation (1e-4) | – | – | 1.1 | 1.7 | 2.4 | 1.6 | 1.6 | 2.0 | 1.4 | 1.6 | 1.5 | 1.5 | 1.3 | 1.9 | 2.0 | 1.1 | 1.3 | 1.2 | 3.1 | 1.7 | 2.6 |
Intensity Change (Log2) | – | – | – | -0.1 | -0.02 | -0.3 | -0.32 | -0.24 | -0.52 | -0.34 | -0.45 | -0.31 | 0.14 | 0.29 | 0.29 | 0.12 | 0.06 | 0.04 | 0.14 | 0.02 | 0.13 |
Localization | RAP60 | RAP140 | RAP220 | RAP300 | RAP380 | RAP460 | RAP540 | RAP620 | RAP700 | HU80 | HU120 | HU160 | rpd3Δ_1 | rpd3Δ_2 | rpd3Δ_3 |
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Cortical Patches | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
Bud | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
Bud Neck | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
Bud Site | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
Cell Periphery | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
Cytoplasm | -1.0 | -0.8 | -1.4 | -2.1 | -2.2 | -2.8 | -2.7 | -3.4 | -2.9 | 1.4 | 0.9 | 1.0 | 0.6 | -0.4 | 0.3 |
Endoplasmic Reticulum | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
Endosome | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
Golgi | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
Mitochondria | 0 | 0 | 0 | 3.0 | 3.0 | 4.0 | 3.1 | 5.0 | 3.1 | 0 | 0 | 0 | 0 | 0 | 0 |
Nucleus | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
Nuclear Periphery | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
Nucleolus | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
Peroxisomes | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
SpindlePole | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
Vacuole | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
Temp | Actin Patch (Sac6-tdTomato) | Cortical Patch (Sla1-GFP) | Late Endosome (Snf7-GFP) | Vacuole (Vph1-GFP) |
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37℃ | ||||
RT |
Gene / Allele | Actin Patch (Sac6-tdTomato) | Cortical Patch (Sla1-GFP) | Late Endosome (Snf7-GFP) | Vacuole (Sac6-tdTomato) |
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Gene | Images |
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Gene | Images |
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