| Standard name | |
|---|---|
| Description | Pho85p cyclin; recruits, activates, and targets Pho85p cyclin-dependent protein kinase to its substrate; PCL10 has a paralog, PCL8, that arose from the whole genome duplication |
Micrographs
Sub-cellular Localization
Yeast GFP Assignment
Protein Abundance
Localization Change
External localization resources
| ensLOC | DeepLoc | |||||||||||||||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|
| Localization | WT1 | WT2 | WT3 | RAP60 | RAP140 | RAP220 | RAP300 | RAP380 | RAP460 | RAP540 | RAP620 | RAP700 | HU80 | HU120 | HU160 | rpd3Δ_1 | rpd3Δ_2 | rpd3Δ_3 | WT1 | WT2 | WT3 | AF100 | AF140 | AF180 |
| Cortical Patches | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 1 | 1 | 0 | 0 | 1 | 0 | 0 | 0 | 0 | 0 | 1 | 0 | 6 | 1 | 3 | 3 |
| Bud | 1 | 3 | 3 | 7 | 5 | 18 | 30 | 11 | 21 | 20 | 26 | 25 | 1 | 0 | 0 | 1 | 1 | 0 | 1 | 0 | 4 | 4 | 8 | 11 |
| Bud Neck | 1 | 1 | 0 | 0 | 1 | 0 | 0 | 0 | 0 | 0 | 0 | 2 | 1 | 1 | 0 | 0 | 0 | 0 | 1 | 1 | 2 | 1 | 0 | 1 |
| Bud Site | 0 | 0 | 0 | 0 | 0 | 1 | 1 | 2 | 1 | 7 | 4 | 2 | 0 | 0 | 0 | 0 | 0 | 0 | – | – | – | – | – | – |
| Cell Periphery | 3 | 1 | 11 | 10 | 10 | 6 | 13 | 14 | 8 | 7 | 9 | 13 | 2 | 8 | 3 | 11 | 15 | 9 | 0 | 0 | 0 | 0 | 0 | 0 |
| Cytoplasm | 189 | 331 | 639 | 487 | 562 | 676 | 749 | 681 | 509 | 619 | 386 | 482 | 470 | 609 | 648 | 238 | 229 | 173 | 199 | 332 | 581 | 493 | 416 | 495 |
| Endoplasmic Reticulum | 0 | 0 | 2 | 1 | 1 | 2 | 2 | 0 | 2 | 0 | 0 | 0 | 0 | 0 | 1 | 12 | 5 | 8 | 5 | 0 | 5 | 0 | 0 | 2 |
| Endosome | 1 | 0 | 1 | 2 | 6 | 2 | 0 | 10 | 2 | 1 | 1 | 2 | 0 | 0 | 2 | 1 | 0 | 1 | 3 | 1 | 3 | 4 | 4 | 4 |
| Golgi | 0 | 0 | 1 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 1 | 0 | 0 | 0 | 0 | 0 | 2 | 0 | 1 | 0 |
| Mitochondria | 4 | 9 | 8 | 59 | 108 | 301 | 497 | 308 | 339 | 481 | 378 | 394 | 0 | 0 | 1 | 5 | 8 | 7 | 5 | 2 | 24 | 4 | 6 | 3 |
| Nucleus | 1 | 0 | 0 | 0 | 1 | 0 | 1 | 3 | 3 | 6 | 3 | 1 | 1 | 1 | 1 | 4 | 3 | 0 | 0 | 0 | 0 | 2 | 0 | 1 |
| Nuclear Periphery | 1 | 0 | 1 | 0 | 0 | 0 | 1 | 1 | 0 | 0 | 0 | 1 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 2 | 0 | 0 | 0 |
| Nucleolus | 0 | 0 | 0 | 1 | 0 | 0 | 4 | 2 | 2 | 0 | 1 | 0 | 0 | 0 | 0 | 0 | 0 | 1 | 0 | 0 | 0 | 0 | 0 | 0 |
| Peroxisomes | 0 | 0 | 1 | 1 | 0 | 0 | 0 | 0 | 1 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 1 | 0 | 0 | 0 |
| SpindlePole | 0 | 0 | 1 | 0 | 3 | 3 | 4 | 8 | 6 | 2 | 1 | 2 | 0 | 0 | 0 | 0 | 0 | 0 | 1 | 0 | 5 | 3 | 3 | 4 |
| Vac/Vac Membrane | 0 | 0 | 4 | 1 | 6 | 8 | 13 | 21 | 12 | 13 | 6 | 9 | 0 | 0 | 1 | 10 | 4 | 11 | 2 | 2 | 2 | 1 | 1 | 3 |
| Unique Cell Count | 195 | 333 | 651 | 502 | 606 | 796 | 1008 | 863 | 700 | 877 | 604 | 703 | 471 | 609 | 653 | 254 | 237 | 187 | 227 | 346 | 654 | 527 | 452 | 537 |
| Labelled Cell Count | 201 | 345 | 672 | 569 | 703 | 1017 | 1315 | 1061 | 907 | 1157 | 815 | 933 | 476 | 619 | 658 | 282 | 265 | 210 | 227 | 346 | 654 | 527 | 452 | 537 |
Yeast GFP Assignment
Protein Abundance
| Screen | WT1 | WT2 | WT3 | RAP60 | RAP140 | RAP220 | RAP300 | RAP380 | RAP460 | RAP540 | RAP620 | RAP700 | HU80 | HU120 | HU160 | rpd3Δ_1 | rpd3Δ_2 | rpd3Δ_3 | AF100 | AF140 | AF180 |
|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|
| Mean Cell GFP Intensity (1e-4) | 4.0 | 4.2 | 4.6 | 4.3 | 4.4 | 3.7 | 3.5 | 3.7 | 3.5 | 3.5 | 3.1 | 3.2 | 5.9 | 6.0 | 5.8 | 6.8 | 6.9 | 7.4 | 5.7 | 5.9 | 6.4 |
| Std Deviation (1e-4) | 0.5 | 0.6 | 1.1 | 1.5 | 1.6 | 1.2 | 1.2 | 1.3 | 1.0 | 1.1 | 0.8 | 0.7 | 0.8 | 0.8 | 1.0 | 1.4 | 1.6 | 1.7 | 1.1 | 1.2 | 1.3 |
| Intensity Change (Log2) | – | – | – | -0.1 | -0.08 | -0.31 | -0.39 | -0.3 | -0.39 | -0.41 | -0.58 | -0.54 | 0.36 | 0.38 | 0.33 | 0.57 | 0.58 | 0.69 | 0.32 | 0.35 | 0.47 |
Localization Change
| Localization | RAP60 | RAP140 | RAP220 | RAP300 | RAP380 | RAP460 | RAP540 | RAP620 | RAP700 | HU80 | HU120 | HU160 | rpd3Δ_1 | rpd3Δ_2 | rpd3Δ_3 |
|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|
| Actin | – | – | – | – | – | – | – | – | – | – | – | – | – | – | – |
| Bud | – | – | – | – | – | – | – | – | – | – | – | – | – | – | – |
| Bud Neck | – | – | – | – | – | – | – | – | – | – | – | – | – | – | – |
| Bud Site | – | – | – | – | – | – | – | – | – | – | – | – | – | – | – |
| Cell Periphery | – | – | – | – | – | – | – | – | – | – | – | – | – | – | – |
| Cyto | – | – | – | – | – | – | – | – | – | – | – | – | – | – | – |
| Endoplasmic Reticulum | – | – | – | – | – | – | – | – | – | – | – | – | – | – | – |
| Endosome | – | – | – | – | – | – | – | – | – | – | – | – | – | – | – |
| Golgi | – | – | – | – | – | – | – | – | – | – | – | – | – | – | – |
| Mitochondria | – | – | – | – | – | – | – | – | – | – | – | – | – | – | – |
| Nuclear Periphery | – | – | – | – | – | – | – | – | – | – | – | – | – | – | – |
| Nuc | – | – | – | – | – | – | – | – | – | – | – | – | – | – | – |
| Nucleolus | – | – | – | – | – | – | – | – | – | – | – | – | – | – | – |
| Peroxisomes | – | – | – | – | – | – | – | – | – | – | – | – | – | – | – |
| SpindlePole | – | – | – | – | – | – | – | – | – | – | – | – | – | – | – |
| Vac | – | – | – | – | – | – | – | – | – | – | – | – | – | – | – |
| Cortical Patches | – | – | – | – | – | – | – | – | – | – | – | – | – | – | – |
| Cytoplasm | – | – | – | – | – | – | – | – | – | – | – | – | – | – | – |
| Nucleus | – | – | – | – | – | – | – | – | – | – | – | – | – | – | – |
| Vacuole | – | – | – | – | – | – | – | – | – | – | – | – | – | – | – |
External localization resources
Endocytosis
| Temp | Actin Patch (Sac6-tdTomato) | Cortical Patch (Sla1-GFP) | Late Endosome (Snf7-GFP) | Vacuole (Vph1-GFP) |
|---|---|---|---|---|
| 37℃ | ||||
| RT |
Cell Cycle Omics
CYCLoPs (Pcl10-GFP)
| Gene / Allele | Actin Patch (Sac6-tdTomato) | Cortical Patch (Sla1-GFP) | Late Endosome (Snf7-GFP) | Vacuole (Sac6-tdTomato) |
|---|
| Gene | Images |
|---|
| Gene | Images |
|---|
Images are not yet available
Images are not yet available
