Standard name
Description Pho85p cyclin; recruits, activates, and targets Pho85p cyclin-dependent protein kinase to its substrate; PCL10 has a paralog, PCL8, that arose from the whole genome duplication

Micrographs

ensLOC DeepLoc
Localization WT1 WT2 WT3 RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3 WT1 WT2 WT3 AF100 AF140 AF180
Cortical Patches 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Bud 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Bud Neck 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Bud Site 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Cell Periphery 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0.06 0.05 0 0 0 0 0 0
Cytoplasm 0.97 0.99 0.98 0.97 0.93 0.85 0.74 0.79 0.73 0.71 0.64 0.69 1.0 1.0 0.99 0.94 0.97 0.93 0.88 0.96 0.89 0.94 0.92 0.92
Endoplasmic Reticulum 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0.05 0 0 0 0 0 0 0 0
Endosome 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Golgi 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Mitochondria 0 0 0 0.12 0.18 0.38 0.49 0.36 0.48 0.55 0.63 0.56 0 0 0 0 0 0 0 0 0 0 0 0
Nucleus 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Nuclear Periphery 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Nucleolus 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Peroxisomes 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
SpindlePole 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Vac/Vac Membrane 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0.06 0 0 0 0 0 0
ensLOC DeepLoc
Localization WT1 WT2 WT3 RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3 WT1 WT2 WT3 AF100 AF140 AF180
Cortical Patches 0 0 0 0 0 0 0 0 1 1 0 0 1 0 0 0 0 0 1 0 6 1 3 3
Bud 1 3 3 7 5 18 30 11 21 20 26 25 1 0 0 1 1 0 1 0 4 4 8 11
Bud Neck 1 1 0 0 1 0 0 0 0 0 0 2 1 1 0 0 0 0 1 1 2 1 0 1
Bud Site 0 0 0 0 0 1 1 2 1 7 4 2 0 0 0 0 0 0
Cell Periphery 3 1 11 10 10 6 13 14 8 7 9 13 2 8 3 11 15 9 0 0 0 0 0 0
Cytoplasm 189 331 639 487 562 676 749 681 509 619 386 482 470 609 648 238 229 173 199 332 581 493 416 495
Endoplasmic Reticulum 0 0 2 1 1 2 2 0 2 0 0 0 0 0 1 12 5 8 5 0 5 0 0 2
Endosome 1 0 1 2 6 2 0 10 2 1 1 2 0 0 2 1 0 1 3 1 3 4 4 4
Golgi 0 0 1 0 0 0 0 0 0 0 0 0 0 0 1 0 0 0 0 0 2 0 1 0
Mitochondria 4 9 8 59 108 301 497 308 339 481 378 394 0 0 1 5 8 7 5 2 24 4 6 3
Nucleus 1 0 0 0 1 0 1 3 3 6 3 1 1 1 1 4 3 0 0 0 0 2 0 1
Nuclear Periphery 1 0 1 0 0 0 1 1 0 0 0 1 0 0 0 0 0 0 0 0 2 0 0 0
Nucleolus 0 0 0 1 0 0 4 2 2 0 1 0 0 0 0 0 0 1 0 0 0 0 0 0
Peroxisomes 0 0 1 1 0 0 0 0 1 0 0 0 0 0 0 0 0 0 0 0 1 0 0 0
SpindlePole 0 0 1 0 3 3 4 8 6 2 1 2 0 0 0 0 0 0 1 0 5 3 3 4
Vac/Vac Membrane 0 0 4 1 6 8 13 21 12 13 6 9 0 0 1 10 4 11 2 2 2 1 1 3
Unique Cell Count 195 333 651 502 606 796 1008 863 700 877 604 703 471 609 653 254 237 187 227 346 654 527 452 537
Labelled Cell Count 201 345 672 569 703 1017 1315 1061 907 1157 815 933 476 619 658 282 265 210 227 346 654 527 452 537


Cytoplasm

(Huh et al. Nature 2003 425:686)
Screen WT1 WT2 WT3 RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3 AF100 AF140 AF180
Mean Cell GFP Intensity (1e-4) 4.0 4.2 4.6 4.3 4.4 3.7 3.5 3.7 3.5 3.5 3.1 3.2 5.9 6.0 5.8 6.8 6.9 7.4 5.7 5.9 6.4
Std Deviation (1e-4) 0.5 0.6 1.1 1.5 1.6 1.2 1.2 1.3 1.0 1.1 0.8 0.7 0.8 0.8 1.0 1.4 1.6 1.7 1.1 1.2 1.3
Intensity Change (Log2) -0.1 -0.08 -0.31 -0.39 -0.3 -0.39 -0.41 -0.58 -0.54 0.36 0.38 0.33 0.57 0.58 0.69 0.32 0.35 0.47


Localization RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3
Actin
Bud
Bud Neck
Bud Site
Cell Periphery
Cyto
Endoplasmic Reticulum
Endosome
Golgi
Mitochondria
Nuclear Periphery
Nuc
Nucleolus
Peroxisomes
SpindlePole
Vac
Cortical Patches
Cytoplasm
Nucleus
Vacuole


Endocytosis
Temp Actin Patch (Sac6-tdTomato) Cortical Patch (Sla1-GFP) Late Endosome (Snf7-GFP) Vacuole (Vph1-GFP)
37℃
RT
Cell Cycle Omics

CYCLoPs (Pcl10-GFP)
Gene / Allele Actin Patch (Sac6-tdTomato) Cortical Patch (Sla1-GFP) Late Endosome (Snf7-GFP) Vacuole (Sac6-tdTomato)
Gene Images
Gene Images
Images are not yet available
Images are not yet available