Standard name
Human Ortholog
Description Essential membrane protein localized at nuclear envelope and SPBs; required for insertion of the newly duplicated spindle pole body into the nuclear envelope; potentially phosphorylated by Cdc28p; MPS2 has a paralog, CSM4, that arose from the whole genome duplication

Micrographs

ensLOC DeepLoc
Localization WT1 WT2 WT3 RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3 WT1 WT2 WT3 AF100 AF140 AF180
Cortical Patches 0 0 0 0 0 0 0 0 0 0 0.17
Bud 0 0 0.1 0.08 0.11 0 0 0 0 0 0
Bud Neck 0 0.06 0.07 0 0 0 0 0 0 0.06 0
Bud Site 0 0 0 0 0.06 0
Cell Periphery 0 0 0 0 0 0 0 0 0 0 0
Cytoplasm 0.44 0.64 0.49 0.48 0.35 0.35 0.09 0.22 0.31 0.27 0.18
Endoplasmic Reticulum 0 0 0 0 0 0.12 0 0 0 0 0
Endosome 0.14 0 0.07 0 0 0.06 0.2 0.06 0.05 0.12 0
Golgi 0 0 0 0 0 0 0 0 0 0 0
Mitochondria 0.06 0.14 0.28 0.46 0.63 0 0.24 0.06 0.21 0.12 0.11
Nucleus 0 0 0 0 0 0 0 0 0 0 0
Nuclear Periphery 0 0 0 0 0 0 0 0 0 0 0
Nucleolus 0 0 0 0 0 0 0 0 0 0.1 0.14
Peroxisomes 0 0 0 0 0 0.06 0 0 0 0 0
SpindlePole 0.35 0.19 0.23 0.22 0.16 0.65 0.3 0.46 0.27 0 0.08
Vac/Vac Membrane 0.05 0 0 0 0 0.06 0 0 0 0 0.1
ensLOC DeepLoc
Localization WT1 WT2 WT3 RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3 WT1 WT2 WT3 AF100 AF140 AF180
Cortical Patches 0 0 0 0 0 0 0 5 6 0 1
Bud 5 5 16 23 25 0 0 3 0 0 0
Bud Neck 4 7 11 11 6 0 0 6 1 0 0
Bud Site 0 0 2 10 13 0
Cell Periphery 0 2 0 0 0 0 0 0 3 0 0
Cytoplasm 58 79 83 131 79 6 1 37 58 2 1
Endoplasmic Reticulum 1 0 0 0 0 2 0 0 0 0 0
Endosome 19 1 12 2 1 1 4 10 9 1 0
Golgi 1 1 0 0 0 0 0 2 1 0 0
Mitochondria 8 17 47 127 143 0 4 10 39 1 0
Nucleus 1 0 1 1 0 0 0 0 0 0 0
Nuclear Periphery 1 0 0 0 0 0 0 0 0 0 0
Nucleolus 0 0 0 1 1 0 0 0 0 1 1
Peroxisomes 1 1 6 8 7 1 0 1 2 0 0
SpindlePole 47 24 38 61 37 11 6 79 50 0 0
Vac/Vac Membrane 6 2 2 2 1 1 0 6 3 0 0
Unique Cell Count 133 124 168 274 226 17 20 174 190 10 8
Labelled Cell Count 152 139 218 377 313 22 20 174 190 10 8


SpindlePole

(Huh et al. Nature 2003 425:686)
Screen WT1 WT2 WT3 RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3 AF100 AF140 AF180
Mean Cell GFP Intensity (1e-4) 4.8 3.8 3.2 2.9 2.9 4.8 5.1
Std Deviation (1e-4) 2.0 1.7 1.2 1.8 1.7 0.4 1.1
Intensity Change (Log2) -0.22 -0.38 -0.37 0.34 0.43


Localization RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3
Actin
Bud
Bud Neck
Bud Site
Cell Periphery
Cyto
Endoplasmic Reticulum
Endosome
Golgi
Mitochondria
Nuclear Periphery
Nuc
Nucleolus
Peroxisomes
SpindlePole
Vac

Images

Circle plot not available

Protein Concentration and Protein Localization Data

R1 R2 R3
G1 Pre-START G1 Post-START S/G2 Metaphase Anaphase Telophase G1 Pre-START G1 Post-START S/G2 Metaphase Anaphase Telophase G1 Pre-START G1 Post-START S/G2 Metaphase Anaphase Telophase
Concentration
Actin 0.1769 0.0023 0.0083 0.0025 0.0075 0.0112
Bud 0.0014 0.0014 0.0005 0.0001 0.0006 0.0002
Bud Neck 0.0012 0.0007 0.0009 0.0005 0.0024 0.0037
Bud Periphery 0.0018 0.0023 0.0008 0.0002 0.0015 0.0008
Bud Site 0.0057 0.0043 0.0034 0.0001 0.0022 0.0002
Cell Periphery 0.0006 0.0001 0.0001 0 0.0001 0.0001
Cytoplasm 0.0127 0.0328 0.022 0.0177 0.0498 0.0306
Cytoplasmic Foci 0.1518 0.1994 0.149 0.2017 0.2412 0.1144
Eisosomes 0.0009 0.0003 0.0001 0.0001 0.0002 0.0002
Endoplasmic Reticulum 0.1425 0.0009 0.0009 0.0006 0.0006 0.0009
Endosome 0.0371 0.0187 0.0208 0.0259 0.015 0.0279
Golgi 0.0294 0.0035 0.0042 0.0029 0.0036 0.0044
Lipid Particles 0.097 0.0206 0.026 0.0483 0.051 0.0184
Mitochondria 0.0063 0.0036 0.0044 0.0007 0.0042 0.0101
None 0.01 0.4151 0.3295 0.276 0.3485 0.4159
Nuclear Periphery 0.0137 0.0034 0.0131 0.014 0.0063 0.0051
Nucleolus 0.0008 0.0033 0.004 0.0016 0.0018 0.0025
Nucleus 0.0011 0.0086 0.0098 0.0023 0.0057 0.0087
Peroxisomes 0.1328 0.0384 0.0409 0.0597 0.0947 0.0268
Punctate Nuclear 0.1726 0.2382 0.3599 0.3442 0.1624 0.3163
Vacuole 0.0025 0.0015 0.001 0.0005 0.0005 0.001
Vacuole Periphery 0.0012 0.0003 0.0005 0.0005 0.0003 0.0004

Sequencing Data

R1 R2
G1 Post-START S/G2 Metaphase Anaphase Telophase G1 Post-START S/G2 Metaphase Anaphase Telophase
Gene Expression 6.1616 4.237 2.5152 5.4931 5.5307 9.4742 7.7113 4.9154 4.377 8.7691
Translational Efficiency 1.2862 1.5238 1.2409 0.8118 1.3083 1.2344 0.6874 0.7392 1.0234 0.9372

Hit Data

Dataset Hit
Protein Concentration
Protein Localization
Gene Expression
Translational Efficiency