Standard name
Description Mannosyltransferase in the N-linked glycosylation pathway; catalyzes two consecutive steps in the N-linked glycosylation pathway; alg2 mutants exhibit temperature-sensitive growth and abnormal accumulation of the lipid-linked oligosaccharide Man2GlcNAc2-PP-Dol; human ALG2 complements the temperature sensitivity and dolichol-linked oligosaccharide biosynthesis defect of the alg2-1 mutant, but mutant form from a patient with CDG-Ii fails to complement

Micrographs

ensLOC DeepLoc
Localization WT1 WT2 WT3 RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3 WT1 WT2 WT3 AF100 AF140 AF180
Cortical Patches 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0.09 0.09 0 0 0 0 0 0
Bud 0 0 0.07 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Bud Neck 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Bud Site 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Cell Periphery 0 0 0 0.06 0 0 0 0 0 0 0 0 0.07 0.07 0.06 0.05 0.11 0 0 0 0 0 0 0
Cytoplasm 0.31 0.38 0.55 0.49 0.62 0.43 0.46 0.48 0.41 0.41 0.27 0.34 0.54 0.6 0.75 0.73 0.71 0.79 0.06 0.14 0.16 0.11 0.13 0.12
Endoplasmic Reticulum 0.54 0.46 0.26 0.33 0.13 0.28 0.12 0.05 0.16 0.11 0.23 0.09 0.41 0.38 0.24 0 0.06 0.06 0.8 0.55 0.58 0.72 0.68 0.53
Endosome 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Golgi 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Mitochondria 0.24 0.14 0.19 0.29 0.11 0.41 0.51 0.49 0.56 0.6 0.65 0.72 0 0 0 0.08 0.06 0 0.06 0.06 0.11 0.06 0.07 0.11
Nucleus 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0.06 0 0 0 0 0 0
Nuclear Periphery 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Nucleolus 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Peroxisomes 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
SpindlePole 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Vac/Vac Membrane 0 0.08 0 0 0.15 0.05 0.07 0.07 0.06 0 0 0 0 0 0 0.11 0.06 0.06 0 0.14 0.05 0 0 0.09
ensLOC DeepLoc
Localization WT1 WT2 WT3 RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3 WT1 WT2 WT3 AF100 AF140 AF180
Cortical Patches 3 0 0 4 2 2 0 1 1 1 3 0 4 5 1 2 9 3 5 3 4 5 5 10
Bud 5 1 8 2 3 1 7 10 7 4 1 6 2 2 1 0 1 0 0 2 0 1 1 2
Bud Neck 0 0 0 0 0 0 0 2 0 0 0 0 0 0 0 3 0 0 2 4 1 2 2 8
Bud Site 0 0 0 0 0 0 0 1 2 0 0 0 0 0 0 0 0 0
Cell Periphery 3 5 3 8 0 7 5 6 8 6 4 9 16 23 16 8 11 1 1 2 2 4 4 11
Cytoplasm 61 65 60 61 54 112 154 207 111 126 61 99 129 187 215 116 71 26 15 30 23 26 38 40
Endoplasmic Reticulum 105 77 29 41 11 72 41 22 44 35 53 27 99 118 70 5 6 2 203 122 83 174 197 183
Endosome 4 0 0 1 2 0 0 0 0 0 0 0 2 3 2 6 3 0 0 1 0 1 2 5
Golgi 0 3 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 1 1 4 0 0 1 1
Mitochondria 47 24 21 36 10 107 169 210 152 184 150 209 3 5 3 13 6 1 14 12 16 13 19 37
Nucleus 0 2 0 1 2 0 0 3 6 2 3 1 0 0 0 4 1 2 0 0 0 0 0 0
Nuclear Periphery 4 2 0 0 2 1 3 4 4 4 5 4 0 0 0 0 0 0 1 1 1 3 3 0
Nucleolus 0 0 0 0 0 0 0 1 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Peroxisomes 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
SpindlePole 0 0 0 1 0 0 2 3 0 0 0 0 0 0 0 0 1 0 0 0 0 0 0 1
Vac/Vac Membrane 8 14 1 3 13 12 25 31 17 13 5 11 7 4 4 17 6 2 6 31 7 5 11 32
Unique Cell Count 194 169 110 124 87 259 334 428 271 305 230 291 239 311 287 159 100 33 255 222 145 244 293 344
Labelled Cell Count 240 193 122 158 99 314 406 501 352 375 285 366 262 347 312 174 115 38 255 222 145 244 293 344


Endoplasmic Reticulum

(Huh et al. Nature 2003 425:686)
Screen WT1 WT2 WT3 RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3 AF100 AF140 AF180
Mean Cell GFP Intensity (1e-4) 5.1 5.4 4.5 4.6 5.6 4.3 3.8 4.0 3.9 3.5 3.6 3.4 6.2 5.8 5.7 5.9 7.4 8.0 6.0 5.7 6.3
Std Deviation (1e-4) 0.6 0.8 0.6 0.9 2.0 0.9 0.7 0.9 0.7 0.7 0.7 0.6 1.2 1.0 1.1 1.3 1.6 1.7 1.4 1.4 1.4
Intensity Change (Log2) 0.04 0.31 -0.08 -0.25 -0.15 -0.22 -0.36 -0.32 -0.38 0.47 0.36 0.34 0.4 0.72 0.83 0.41 0.34 0.49


Localization RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3
Cortical Patches 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Bud 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Bud Neck 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Bud Site 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Cell Periphery 0 0 0 0 0 0 0 0 0 1.5 1.7 1.2 0 2.4 0
Cytoplasm -0.8 1.1 -2.0 -1.5 -1.2 -2.4 -2.4 -5.0 -3.7 -0.1 1.0 3.9 3.1 2.5 2.5
Endoplasmic Reticulum 0 -2.4 0 0 0 0 0 0 0 2.7 2.2 -0.4 -5.6 -4.0 -2.5
Endosome 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Golgi 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Mitochondria 0 -1.5 0 0 0 0 0 0 0 -6.1 -6.5 -6.8 -2.6 -2.8 -2.2
Nucleus 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Nuclear Periphery 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Nucleolus 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Peroxisomes 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
SpindlePole 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Vacuole 0 3.8 0 2.5 2.5 2.2 0 0 0 0 0 0 3.2 0 0


Endocytosis
Temp Actin Patch (Sac6-tdTomato) Cortical Patch (Sla1-GFP) Late Endosome (Snf7-GFP) Vacuole (Vph1-GFP)
37℃
RT
Cell Cycle Omics

CYCLoPs (Alg2-GFP)
Gene / Allele Actin Patch (Sac6-tdTomato) Cortical Patch (Sla1-GFP) Late Endosome (Snf7-GFP) Vacuole (Sac6-tdTomato)
Gene Images
Gene Images
Images are not yet available
Images are not yet available