Standard name | |
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Human Ortholog | |
Description | Mannosyltransferase in the N-linked glycosylation pathway; catalyzes two consecutive steps in the N-linked glycosylation pathway; alg2 mutants exhibit temperature-sensitive growth and abnormal accumulation of the lipid-linked oligosaccharide Man2GlcNAc2-PP-Dol; human ALG2 complements the temperature sensitivity and dolichol-linked oligosaccharide biosynthesis defect of the alg2-1 mutant, but mutant form from a patient with CDG-Ii fails to complement |
ensLOC | DeepLoc | |||||||||||||||||||||||
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Localization | WT1 | WT2 | WT3 | RAP60 | RAP140 | RAP220 | RAP300 | RAP380 | RAP460 | RAP540 | RAP620 | RAP700 | HU80 | HU120 | HU160 | rpd3Δ_1 | rpd3Δ_2 | rpd3Δ_3 | WT1 | WT2 | WT3 | AF100 | AF140 | AF180 |
Cortical Patches | 3 | 0 | 0 | 4 | 2 | 2 | 0 | 1 | 1 | 1 | 3 | 0 | 4 | 5 | 1 | 2 | 9 | 3 | 5 | 3 | 4 | 5 | 5 | 10 |
Bud | 5 | 1 | 8 | 2 | 3 | 1 | 7 | 10 | 7 | 4 | 1 | 6 | 2 | 2 | 1 | 0 | 1 | 0 | 0 | 2 | 0 | 1 | 1 | 2 |
Bud Neck | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 2 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 3 | 0 | 0 | 2 | 4 | 1 | 2 | 2 | 8 |
Bud Site | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 1 | 2 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | – | – | – | – | – | – |
Cell Periphery | 3 | 5 | 3 | 8 | 0 | 7 | 5 | 6 | 8 | 6 | 4 | 9 | 16 | 23 | 16 | 8 | 11 | 1 | 1 | 2 | 2 | 4 | 4 | 11 |
Cytoplasm | 61 | 65 | 60 | 61 | 54 | 112 | 154 | 207 | 111 | 126 | 61 | 99 | 129 | 187 | 215 | 116 | 71 | 26 | 15 | 30 | 23 | 26 | 38 | 40 |
Endoplasmic Reticulum | 105 | 77 | 29 | 41 | 11 | 72 | 41 | 22 | 44 | 35 | 53 | 27 | 99 | 118 | 70 | 5 | 6 | 2 | 203 | 122 | 83 | 174 | 197 | 183 |
Endosome | 4 | 0 | 0 | 1 | 2 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 2 | 3 | 2 | 6 | 3 | 0 | 0 | 1 | 0 | 1 | 2 | 5 |
Golgi | 0 | 3 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 1 | 1 | 4 | 0 | 0 | 1 | 1 |
Mitochondria | 47 | 24 | 21 | 36 | 10 | 107 | 169 | 210 | 152 | 184 | 150 | 209 | 3 | 5 | 3 | 13 | 6 | 1 | 14 | 12 | 16 | 13 | 19 | 37 |
Nucleus | 0 | 2 | 0 | 1 | 2 | 0 | 0 | 3 | 6 | 2 | 3 | 1 | 0 | 0 | 0 | 4 | 1 | 2 | 0 | 0 | 0 | 0 | 0 | 0 |
Nuclear Periphery | 4 | 2 | 0 | 0 | 2 | 1 | 3 | 4 | 4 | 4 | 5 | 4 | 0 | 0 | 0 | 0 | 0 | 0 | 1 | 1 | 1 | 3 | 3 | 0 |
Nucleolus | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 1 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
Peroxisomes | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
SpindlePole | 0 | 0 | 0 | 1 | 0 | 0 | 2 | 3 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 1 | 0 | 0 | 0 | 0 | 0 | 0 | 1 |
Vac/Vac Membrane | 8 | 14 | 1 | 3 | 13 | 12 | 25 | 31 | 17 | 13 | 5 | 11 | 7 | 4 | 4 | 17 | 6 | 2 | 6 | 31 | 7 | 5 | 11 | 32 |
Unique Cell Count | 194 | 169 | 110 | 124 | 87 | 259 | 334 | 428 | 271 | 305 | 230 | 291 | 239 | 311 | 287 | 159 | 100 | 33 | 255 | 222 | 145 | 244 | 293 | 344 |
Labelled Cell Count | 240 | 193 | 122 | 158 | 99 | 314 | 406 | 501 | 352 | 375 | 285 | 366 | 262 | 347 | 312 | 174 | 115 | 38 | 255 | 222 | 145 | 244 | 293 | 344 |
Screen | WT1 | WT2 | WT3 | RAP60 | RAP140 | RAP220 | RAP300 | RAP380 | RAP460 | RAP540 | RAP620 | RAP700 | HU80 | HU120 | HU160 | rpd3Δ_1 | rpd3Δ_2 | rpd3Δ_3 | AF100 | AF140 | AF180 |
---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|
Mean Cell GFP Intensity (1e-4) | 5.1 | 5.4 | 4.5 | 4.6 | 5.6 | 4.3 | 3.8 | 4.0 | 3.9 | 3.5 | 3.6 | 3.4 | 6.2 | 5.8 | 5.7 | 5.9 | 7.4 | 8.0 | 6.0 | 5.7 | 6.3 |
Std Deviation (1e-4) | 0.6 | 0.8 | 0.6 | 0.9 | 2.0 | 0.9 | 0.7 | 0.9 | 0.7 | 0.7 | 0.7 | 0.6 | 1.2 | 1.0 | 1.1 | 1.3 | 1.6 | 1.7 | 1.4 | 1.4 | 1.4 |
Intensity Change (Log2) | – | – | – | 0.04 | 0.31 | -0.08 | -0.25 | -0.15 | -0.22 | -0.36 | -0.32 | -0.38 | 0.47 | 0.36 | 0.34 | 0.4 | 0.72 | 0.83 | 0.41 | 0.34 | 0.49 |
Localization | RAP60 | RAP140 | RAP220 | RAP300 | RAP380 | RAP460 | RAP540 | RAP620 | RAP700 | HU80 | HU120 | HU160 | rpd3Δ_1 | rpd3Δ_2 | rpd3Δ_3 |
---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|
Cortical Patches | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
Bud | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
Bud Neck | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
Bud Site | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
Cell Periphery | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 1.5 | 1.7 | 1.2 | 0 | 2.4 | 0 |
Cytoplasm | -0.8 | 1.1 | -2.0 | -1.5 | -1.2 | -2.4 | -2.4 | -5.0 | -3.7 | -0.1 | 1.0 | 3.9 | 3.1 | 2.5 | 2.5 |
Endoplasmic Reticulum | 0 | -2.4 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 2.7 | 2.2 | -0.4 | -5.6 | -4.0 | -2.5 |
Endosome | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
Golgi | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
Mitochondria | 0 | -1.5 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | -6.1 | -6.5 | -6.8 | -2.6 | -2.8 | -2.2 |
Nucleus | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
Nuclear Periphery | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
Nucleolus | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
Peroxisomes | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
SpindlePole | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
Vacuole | 0 | 3.8 | 0 | 2.5 | 2.5 | 2.2 | 0 | 0 | 0 | 0 | 0 | 0 | 3.2 | 0 | 0 |
R1 | R2 | R3 | ||||||||||||||||
---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|
G1 Pre-START | G1 Post-START | S/G2 | Metaphase | Anaphase | Telophase | G1 Pre-START | G1 Post-START | S/G2 | Metaphase | Anaphase | Telophase | G1 Pre-START | G1 Post-START | S/G2 | Metaphase | Anaphase | Telophase | |
Concentration | – | – | – | – | – | – | – | – | – | – | – | – | – | – | – | – | – | – |
Actin | 0.032 | 0.0046 | 0.0032 | 0.0029 | 0.0015 | 0.0024 | 0.0077 | 0.0004 | 0.0034 | 0.0031 | 0.0004 | 0.0033 | – | – | – | – | – | – |
Bud | 0.0002 | 0.0005 | 0.0003 | 0.0003 | 0 | 0.0001 | 0.0006 | 0 | 0 | 0.0001 | 0 | 0.0001 | – | – | – | – | – | – |
Bud Neck | 0.0004 | 0.0008 | 0.0054 | 0.0015 | 0.0009 | 0.0023 | 0.0011 | 0.0001 | 0.0019 | 0.0016 | 0 | 0.0051 | – | – | – | – | – | – |
Bud Periphery | 0.0003 | 0.0008 | 0.0012 | 0.0012 | 0.0001 | 0.0001 | 0.0008 | 0.0001 | 0 | 0.0002 | 0 | 0.0002 | – | – | – | – | – | – |
Bud Site | 0.001 | 0.0199 | 0.0026 | 0.0003 | 0.0001 | 0.0001 | 0.0074 | 0.0002 | 0.0002 | 0.0002 | 0 | 0.0003 | – | – | – | – | – | – |
Cell Periphery | 0.0012 | 0.0047 | 0.0053 | 0.0035 | 0.0007 | 0.0008 | 0.0008 | 0.0031 | 0.0079 | 0.0039 | 0 | 0.0012 | – | – | – | – | – | – |
Cytoplasm | 0.1348 | 0.5193 | 0.2614 | 0.199 | 0.147 | 0.2787 | 0.0755 | 0.125 | 0.2735 | 0.1517 | 0.0225 | 0.2887 | – | – | – | – | – | – |
Cytoplasmic Foci | 0.0059 | 0.0067 | 0.0036 | 0.0033 | 0.0088 | 0.01 | 0.0251 | 0.0047 | 0.0154 | 0.008 | 0.0018 | 0.014 | – | – | – | – | – | – |
Eisosomes | 0.0006 | 0.0003 | 0.0001 | 0.0001 | 0.0001 | 0.0001 | 0.0007 | 0.0001 | 0.0001 | 0.0004 | 0.0003 | 0.0014 | – | – | – | – | – | – |
Endoplasmic Reticulum | 0.6398 | 0.3184 | 0.6071 | 0.7261 | 0.6614 | 0.5892 | 0.0052 | 0.6536 | 0.6 | 0.5885 | 0.0004 | 0.2172 | – | – | – | – | – | – |
Endosome | 0.0206 | 0.0088 | 0.0104 | 0.0107 | 0.0353 | 0.0141 | 0.0538 | 0.0457 | 0.014 | 0.0071 | 0.0002 | 0.0077 | – | – | – | – | – | – |
Golgi | 0.0074 | 0.0011 | 0.0012 | 0.001 | 0.002 | 0.0014 | 0.0136 | 0.0017 | 0.0029 | 0.0033 | 0 | 0.0009 | – | – | – | – | – | – |
Lipid Particles | 0.0195 | 0.0044 | 0.0042 | 0.003 | 0.0083 | 0.0038 | 0.0965 | 0.0101 | 0.0476 | 0.0058 | 0.0003 | 0.008 | – | – | – | – | – | – |
Mitochondria | 0.0072 | 0.0004 | 0.0004 | 0.0006 | 0.001 | 0.0008 | 0.0119 | 0.0007 | 0.0001 | 0.0005 | 0.0001 | 0.0014 | – | – | – | – | – | – |
None | 0.0931 | 0.0863 | 0.0719 | 0.0048 | 0.0867 | 0.0652 | 0.5103 | 0.1128 | 0.0137 | 0.2099 | 0.9696 | 0.3464 | – | – | – | – | – | – |
Nuclear Periphery | 0.0176 | 0.0099 | 0.0134 | 0.0244 | 0.0281 | 0.017 | 0.0077 | 0.0132 | 0.0096 | 0.0066 | 0.0008 | 0.0095 | – | – | – | – | – | – |
Nucleolus | 0.0004 | 0.0003 | 0.0001 | 0.0002 | 0.0002 | 0.0002 | 0.0036 | 0.0001 | 0 | 0.0003 | 0.0004 | 0.025 | – | – | – | – | – | – |
Nucleus | 0.002 | 0.0044 | 0.0022 | 0.0052 | 0.0076 | 0.0055 | 0.0626 | 0.0013 | 0.0011 | 0.0021 | 0.0007 | 0.0213 | – | – | – | – | – | – |
Peroxisomes | 0.0073 | 0.0002 | 0.0001 | 0.0001 | 0.0001 | 0.0002 | 0.0162 | 0 | 0.0002 | 0.0002 | 0.0001 | 0.001 | – | – | – | – | – | – |
Punctate Nuclear | 0.0042 | 0.0013 | 0.0004 | 0.0021 | 0.003 | 0.0019 | 0.0889 | 0.0004 | 0.0008 | 0.0015 | 0.0021 | 0.0449 | – | – | – | – | – | – |
Vacuole | 0.0035 | 0.0066 | 0.005 | 0.0088 | 0.0058 | 0.0057 | 0.0073 | 0.0219 | 0.0071 | 0.0048 | 0.0001 | 0.0019 | – | – | – | – | – | – |
Vacuole Periphery | 0.0012 | 0.0004 | 0.0005 | 0.001 | 0.0013 | 0.0006 | 0.0027 | 0.0048 | 0.0003 | 0.0004 | 0 | 0.0005 | – | – | – | – | – | – |
R1 | R2 | |||||||||
---|---|---|---|---|---|---|---|---|---|---|
G1 Post-START | S/G2 | Metaphase | Anaphase | Telophase | G1 Post-START | S/G2 | Metaphase | Anaphase | Telophase | |
Gene Expression | 38.6775 | 25.4422 | 19.3633 | 22.2258 | 36.4276 | 34.28 | 31.6611 | 25.821 | 21.2633 | 34.9957 |
Translational Efficiency | 1.0505 | 1.245 | 1.0698 | 0.9341 | 0.7021 | 0.9794 | 1.0057 | 0.7815 | 0.8701 | 0.8696 |
Dataset | Hit |
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Protein Concentration | – |
Protein Localization | ✘ |
Gene Expression | ✔ |
Translational Efficiency | ✘ |
Temp | Actin Patch (Sac6-tdTomato) | Cortical Patch (Sla1-GFP) | Late Endosome (Snf7-GFP) | Vacuole (Vph1-GFP) |
---|---|---|---|---|
37℃ | ||||
RT |
Gene / Allele | Actin Patch (Sac6-tdTomato) | Cortical Patch (Sla1-GFP) | Late Endosome (Snf7-GFP) | Vacuole (Sac6-tdTomato) |
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Gene | Images |
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Gene | Images |
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