Standard name
Human Ortholog
Description Protein involved in proteolytic activation of Rim101p; part of response to alkaline pH; interacts with ESCRT-1 subunits Stp22p and Vps28p; essential for anaerobic growth; member of the arrestin-related trafficking adaptor family

Micrographs

ensLOC DeepLoc
Localization WT1 WT2 WT3 RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3 WT1 WT2 WT3 AF100 AF140 AF180
Cortical Patches 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Bud 0 0 0 0 0 0 0 0 0 0 0 0.07 0 0 0 0 0 0 0 0 0
Bud Neck 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Bud Site 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Cell Periphery 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Cytoplasm 0.98 0.87 0.86 0.33 0.86 0.92 0.93 0.86 0.72 0.78 0.65 0.62 0.84 0.9 0.89 0.76 0.71 0.81 0.76 0.8 0.87
Endoplasmic Reticulum 0 0 0 0 0 0 0 0 0 0 0 0 0.05 0 0 0 0 0 0 0 0
Endosome 0 0 0 0.08 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Golgi 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Mitochondria 0 0.12 0 0.08 0 0 0 0.08 0.27 0.24 0.42 0.31 0 0 0 0.07 0 0 0 0 0
Nucleus 0 0.05 0 0.08 0 0 0 0 0.06 0 0.09 0.07 0.07 0 0 0 0.14 0.09 0.14 0.07 0
Nuclear Periphery 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Nucleolus 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Peroxisomes 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
SpindlePole 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Vac/Vac Membrane 0 0.08 0.14 0.58 0.17 0 0.06 0.07 0 0.05 0 0.08 0.05 0.16 0.09 0 0.07 0 0 0.05 0.06
ensLOC DeepLoc
Localization WT1 WT2 WT3 RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3 WT1 WT2 WT3 AF100 AF140 AF180
Cortical Patches 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Bud 0 2 0 0 0 0 1 0 4 2 0 7 0 2 0 2 1 0 0 2 1
Bud Neck 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 1 0 2 2 1
Bud Site 0 0 0 0 0 0 0 0 0 0 1 1 0 0 0
Cell Periphery 0 0 0 0 1 0 0 2 0 0 0 0 0 0 1 0 0 0 0 0 0
Cytoplasm 64 67 36 8 159 124 228 172 102 89 73 63 51 133 178 70 92 60 104 133 192
Endoplasmic Reticulum 0 0 1 0 0 0 0 0 1 0 2 0 3 0 4 0 0 1 1 2 0
Endosome 0 0 0 2 1 1 1 0 0 0 0 1 0 1 2 0 0 0 1 1 2
Golgi 0 0 0 1 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Mitochondria 2 9 0 2 2 5 8 16 39 27 47 32 0 0 0 6 1 0 0 0 0
Nucleus 1 4 0 2 3 3 7 6 8 4 10 7 4 2 5 3 18 6 19 11 4
Nuclear Periphery 0 0 0 0 0 0 0 0 1 0 0 0 0 0 0 0 0 0 0 0 0
Nucleolus 0 0 0 0 0 1 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Peroxisomes 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
SpindlePole 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 2
Vac/Vac Membrane 1 6 6 14 32 4 15 15 3 6 2 8 3 24 19 3 9 1 5 8 12
Unique Cell Count 65 77 42 24 185 135 244 201 142 114 112 102 61 148 201 93 130 74 138 167 222
Labelled Cell Count 68 88 43 29 198 138 260 211 158 128 135 119 61 162 209 93 130 74 138 167 222


Cytoplasm, Nucleus

(Huh et al. Nature 2003 425:686)
Screen WT1 WT2 WT3 RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3 AF100 AF140 AF180
Mean Cell GFP Intensity (1e-4) 5.8 4.9 7.3 9.2 8.0 6.0 6.3 6.3 5.0 5.4 4.9 4.9 8.1 7.8 7.8 7.9 9.0 8.1
Std Deviation (1e-4) 2.0 0.8 0.9 2.4 1.6 1.0 1.8 1.9 1.3 1.4 1.1 0.9 1.5 1.8 1.5 1.7 2.0 1.3
Intensity Change (Log2) 0.33 0.12 -0.29 -0.23 -0.21 -0.56 -0.46 -0.58 -0.58 0.15 0.09 0.08 0.1 0.29 0.15


Localization RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3
Actin
Bud
Bud Neck
Bud Site
Cell Periphery
Cyto
Endoplasmic Reticulum
Endosome
Golgi
Mitochondria
Nuclear Periphery
Nuc
Nucleolus
Peroxisomes
SpindlePole
Vac
Cortical Patches
Cytoplasm
Nucleus
Vacuole

Images

Circle plot not available

Protein Concentration and Protein Localization Data

R1 R2 R3
G1 Pre-START G1 Post-START S/G2 Metaphase Anaphase Telophase G1 Pre-START G1 Post-START S/G2 Metaphase Anaphase Telophase G1 Pre-START G1 Post-START S/G2 Metaphase Anaphase Telophase
Concentration 3.6619 4.0555 4.1937 3.6181 3.7442 3.7911 6.0544 6.6355 6.8024 6.8303 6.0254 6.7617 3.8948 4.8123 4.3671 4.8825 3.66 4.5646
Actin 0.0209 0.0004 0.0205 0.0025 0.0026 0.0029 0.0013 0.0054 0.0137 0.0015 0.0014 0.0009 0.0281 0.0004 0.0196 0.0009 0.0127 0.0004
Bud 0.0003 0.0001 0.0009 0.0001 0.0001 0.0006 0.0002 0.0002 0.0004 0.0002 0.0001 0.0002 0.0004 0.0001 0.0005 0.0002 0.0002 0.0001
Bud Neck 0.001 0.0009 0.001 0.0012 0.001 0.0052 0.0006 0.0008 0.0015 0.0019 0.0017 0.0032 0.0014 0.0007 0.0015 0.0017 0.0017 0.0032
Bud Periphery 0.0005 0 0.0009 0 0.0001 0.0008 0.0002 0.0001 0.0003 0.0001 0 0.0001 0.0004 0 0.0005 0 0.0003 0
Bud Site 0.0025 0.0028 0.0047 0.0002 0.0001 0.0002 0.0009 0.0012 0.0034 0.0007 0.0003 0.0002 0.0018 0.0006 0.0081 0.0005 0.0004 0.0001
Cell Periphery 0.0004 0.0001 0.0002 0 0 0 0.0002 0.0001 0.0001 0.0001 0.0001 0 0.0002 0.0001 0.0002 0.0001 0.0001 0
Cytoplasm 0.7397 0.7512 0.6549 0.7376 0.7997 0.7065 0.7509 0.6535 0.715 0.6816 0.7263 0.7469 0.7474 0.8026 0.7393 0.6706 0.7642 0.8097
Cytoplasmic Foci 0.0085 0.0021 0.0117 0.0042 0.0035 0.004 0.0113 0.0046 0.0164 0.0031 0.0254 0.0124 0.0104 0.0011 0.0083 0.003 0.0056 0.0026
Eisosomes 0.0003 0 0.0001 0 0.0001 0 0 0 0.0001 0 0 0 0.0002 0 0.0002 0.0001 0.0001 0
Endoplasmic Reticulum 0.0149 0.0093 0.0096 0.0057 0.0078 0.0055 0.0153 0.007 0.0159 0.0117 0.0089 0.0084 0.0172 0.0049 0.0099 0.0088 0.0095 0.0066
Endosome 0.0095 0.0024 0.0086 0.0019 0.0137 0.0053 0.0167 0.0031 0.0131 0.0026 0.0162 0.0081 0.0052 0.0012 0.0111 0.0036 0.026 0.0019
Golgi 0.0045 0.0001 0.0041 0.001 0.0038 0.0006 0.0012 0.0005 0.0024 0.0002 0.001 0.0004 0.0029 0 0.0027 0.0002 0.0222 0
Lipid Particles 0.0051 0.0001 0.0029 0.0018 0.0097 0.0031 0.0019 0.0002 0.0027 0.0002 0.0005 0.0008 0.0025 0.0001 0.0011 0.0006 0.0068 0
Mitochondria 0.0042 0.0002 0.0038 0.0003 0.005 0.0027 0.0007 0.0005 0.0012 0.0003 0.0003 0.0005 0.0053 0.0001 0.0015 0.0003 0.0138 0.0002
None 0.0169 0.0208 0.0102 0.0014 0.008 0.0053 0.0439 0.0043 0.0275 0.0513 0.0611 0.037 0.0273 0.0047 0.0103 0.0438 0.0027 0.0111
Nuclear Periphery 0.0214 0.0175 0.0221 0.01 0.0185 0.011 0.0167 0.0154 0.0158 0.0136 0.0123 0.0075 0.0122 0.0085 0.0133 0.0145 0.0371 0.0075
Nucleolus 0.0007 0.0002 0.0004 0.0001 0.0002 0.0003 0.0027 0.0002 0.0004 0.0004 0.0002 0.0002 0.0003 0.0001 0.0006 0.0007 0.0001 0.0001
Nucleus 0.1387 0.1874 0.2321 0.1794 0.1184 0.2376 0.1228 0.297 0.1531 0.2234 0.1334 0.1656 0.1279 0.1718 0.1546 0.2428 0.0784 0.1525
Peroxisomes 0.0029 0.0001 0.0044 0.048 0.0012 0.0005 0.0017 0.0003 0.007 0.0001 0.0016 0.0008 0.0035 0 0.0026 0.0002 0.0016 0
Punctate Nuclear 0.004 0.0027 0.0041 0.0031 0.0036 0.0056 0.006 0.0036 0.007 0.0051 0.0073 0.0044 0.0031 0.0024 0.012 0.006 0.0025 0.0026
Vacuole 0.0026 0.0015 0.0022 0.0014 0.0015 0.002 0.0037 0.0017 0.0027 0.0017 0.0015 0.0018 0.0018 0.0005 0.0016 0.0014 0.004 0.0011
Vacuole Periphery 0.0005 0.0002 0.0004 0.0002 0.0014 0.0003 0.0009 0.0002 0.0004 0.0002 0.0002 0.0002 0.0003 0.0001 0.0003 0.0002 0.01 0.0001

Sequencing Data

R1 R2
G1 Post-START S/G2 Metaphase Anaphase Telophase G1 Post-START S/G2 Metaphase Anaphase Telophase
Gene Expression 6.0962 7.8716 4.9425 6.6331 4.3911 8.8007 6.1223 5.9916 6.5787 7.8505
Translational Efficiency 0.5532 0.4722 0.6032 0.593 0.8285 0.4812 0.7215 0.7492 0.6807 0.6691

Hit Data

Dataset Hit
Protein Concentration
Protein Localization
Gene Expression
Translational Efficiency
Standard name
Human Ortholog
Description Protein involved in proteolytic activation of Rim101p; part of response to alkaline pH; interacts with ESCRT-1 subunits Stp22p and Vps28p; essential for anaerobic growth; member of the arrestin-related trafficking adaptor family
Localization
Cell Percentages cytoplasm (38%), nucleus (0%), mixed (46%)
Cell Cycle Regulation No
Subcompartmental Group N/A

Rim8

Rim8


Nearest neighbours ( genes)

correlation threshold

0.6

1.0

0.80


Biological Process (Gene Ontology)

Molecular Function (Gene Ontology)

Cellular Component (Gene Ontology)


Endocytosis
Temp Actin Patch (Sac6-tdTomato) Cortical Patch (Sla1-GFP) Late Endosome (Snf7-GFP) Vacuole (Vph1-GFP)
37℃
RT
Cell Cycle Omics

CYCLoPs (Rim8-GFP)
Gene / Allele Actin Patch (Sac6-tdTomato) Cortical Patch (Sla1-GFP) Late Endosome (Snf7-GFP) Vacuole (Sac6-tdTomato)
Gene Images
Gene Images
Images are not yet available
Images are not yet available