Standard name
Description Protein involved in proteolytic activation of Rim101p; part of response to alkaline pH; interacts with ESCRT-1 subunits Stp22p and Vps28p; essential for anaerobic growth; member of the arrestin-related trafficking adaptor family
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Micrographs

ensLOC DeepLoc
Localization WT1 WT2 WT3 RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3 WT1 WT2 WT3 AF100 AF140 AF180
Cortical Patches 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 – – – 0 0 0 0 0 0
Bud 0 0 0 0 0 0 0 0 0 0 0 0.07 0 0 0 – – – 0 0 0 0 0 0
Bud Neck 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 – – – 0 0 0 0 0 0
Bud Site 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 – – – – – – – – –
Cell Periphery 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 – – – 0 0 0 0 0 0
Cytoplasm 0.98 0.87 0.86 0.33 0.86 0.92 0.93 0.86 0.72 0.78 0.65 0.62 0.84 0.9 0.89 – – – 0.76 0.71 0.81 0.76 0.8 0.87
Endoplasmic Reticulum 0 0 0 0 0 0 0 0 0 0 0 0 0.05 0 0 – – – 0 0 0 0 0 0
Endosome 0 0 0 0.08 0 0 0 0 0 0 0 0 0 0 0 – – – 0 0 0 0 0 0
Golgi 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 – – – 0 0 0 0 0 0
Mitochondria 0 0.12 0 0.08 0 0 0 0.08 0.27 0.24 0.42 0.31 0 0 0 – – – 0.07 0 0 0 0 0
Nucleus 0 0.05 0 0.08 0 0 0 0 0.06 0 0.09 0.07 0.07 0 0 – – – 0 0.14 0.09 0.14 0.07 0
Nuclear Periphery 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 – – – 0 0 0 0 0 0
Nucleolus 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 – – – 0 0 0 0 0 0
Peroxisomes 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 – – – 0 0 0 0 0 0
SpindlePole 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 – – – 0 0 0 0 0 0
Vac/Vac Membrane 0 0.08 0.14 0.58 0.17 0 0.06 0.07 0 0.05 0 0.08 0.05 0.16 0.09 – – – 0 0.07 0 0 0.05 0.06
ensLOC DeepLoc
Localization WT1 WT2 WT3 RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3 WT1 WT2 WT3 AF100 AF140 AF180
Cortical Patches 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 – – – 0 0 0 0 0 0
Bud 0 2 0 0 0 0 1 0 4 2 0 7 0 2 0 – – – 2 1 0 0 2 1
Bud Neck 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 – – – 0 1 0 2 2 1
Bud Site 0 0 0 0 0 0 0 0 0 0 1 1 0 0 0 – – – – – – – – –
Cell Periphery 0 0 0 0 1 0 0 2 0 0 0 0 0 0 1 – – – 0 0 0 0 0 0
Cytoplasm 64 67 36 8 159 124 228 172 102 89 73 63 51 133 178 – – – 70 92 60 104 133 192
Endoplasmic Reticulum 0 0 1 0 0 0 0 0 1 0 2 0 3 0 4 – – – 0 0 1 1 2 0
Endosome 0 0 0 2 1 1 1 0 0 0 0 1 0 1 2 – – – 0 0 0 1 1 2
Golgi 0 0 0 1 0 0 0 0 0 0 0 0 0 0 0 – – – 0 0 0 0 0 0
Mitochondria 2 9 0 2 2 5 8 16 39 27 47 32 0 0 0 – – – 6 1 0 0 0 0
Nucleus 1 4 0 2 3 3 7 6 8 4 10 7 4 2 5 – – – 3 18 6 19 11 4
Nuclear Periphery 0 0 0 0 0 0 0 0 1 0 0 0 0 0 0 – – – 0 0 0 0 0 0
Nucleolus 0 0 0 0 0 1 0 0 0 0 0 0 0 0 0 – – – 0 0 0 0 0 0
Peroxisomes 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 – – – 0 0 0 0 0 0
SpindlePole 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 – – – 0 0 0 0 0 2
Vac/Vac Membrane 1 6 6 14 32 4 15 15 3 6 2 8 3 24 19 – – – 3 9 1 5 8 12
Unique Cell Count 65 77 42 24 185 135 244 201 142 114 112 102 61 148 201 93 130 74 138 167 222
Labelled Cell Count 68 88 43 29 198 138 260 211 158 128 135 119 61 162 209 93 130 74 138 167 222


Cytoplasm, Nucleus

(Huh et al. Nature 2003 425:686)
Screen WT1 WT2 WT3 RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3 AF100 AF140 AF180
Mean Cell GFP Intensity (1e-4) 5.8 4.9 7.3 9.2 8.0 6.0 6.3 6.3 5.0 5.4 4.9 4.9 8.1 7.8 7.8 – – – 7.9 9.0 8.1
Std Deviation (1e-4) 2.0 0.8 0.9 2.4 1.6 1.0 1.8 1.9 1.3 1.4 1.1 0.9 1.5 1.8 1.5 – – – 1.7 2.0 1.3
Intensity Change (Log2) – – – 0.33 0.12 -0.29 -0.23 -0.21 -0.56 -0.46 -0.58 -0.58 0.15 0.09 0.08 – – – 0.1 0.29 0.15


Localization RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3
Actin – – – – – – – – – – – – – – –
Bud – – – – – – – – – – – – – – –
Bud Neck – – – – – – – – – – – – – – –
Bud Site – – – – – – – – – – – – – – –
Cell Periphery – – – – – – – – – – – – – – –
Cyto – – – – – – – – – – – – – – –
Endoplasmic Reticulum – – – – – – – – – – – – – – –
Endosome – – – – – – – – – – – – – – –
Golgi – – – – – – – – – – – – – – –
Mitochondria – – – – – – – – – – – – – – –
Nuclear Periphery – – – – – – – – – – – – – – –
Nuc – – – – – – – – – – – – – – –
Nucleolus – – – – – – – – – – – – – – –
Peroxisomes – – – – – – – – – – – – – – –
SpindlePole – – – – – – – – – – – – – – –
Vac – – – – – – – – – – – – – – –
Cortical Patches – – – – – – – – – – – – – – –
Cytoplasm – – – – – – – – – – – – – – –
Nucleus – – – – – – – – – – – – – – –
Vacuole – – – – – – – – – – – – – – –