Standard name
Human Ortholog
Description Adhesion subunit of a-agglutinin of a-cells; C-terminal sequence acts as a ligand for alpha-agglutinin (Sag1p) during agglutination, modified with O-linked oligomannosyl chains, linked to anchorage subunit Aga1p via two disulfide bonds
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Micrographs

ensLOC DeepLoc
Localization WT1 WT2 WT3 RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3 WT1 WT2 WT3 AF100 AF140 AF180
Cortical Patches 0 0 0 0 0 0 – 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Bud 0 0 0 0 0 0 – 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Bud Neck 0 0 0 0 0 0 – 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Bud Site 0 0 0 0 0 0 – 0 0 0 0 0 0 0 0 0 0 0 – – – – – –
Cell Periphery 0 0 0 0 0 0 – 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Cytoplasm 0.34 0.56 0.59 0.72 0.63 0.73 – 0.77 0.79 0.8 0.79 0.76 0.9 0.95 0.98 0.95 0.94 0.91 0.09 0.12 0.09 0 0 0
Endoplasmic Reticulum 0 0 0 0 0 0 – 0 0 0 0 0 0 0 0 0.06 0.05 0.08 0 0 0 0 0 0
Endosome 0.06 0 0 0 0 0 – 0 0 0 0 0 0 0 0 0 0 0 0 0.05 0 0.27 0.12 0.15
Golgi 0 0 0 0 0 0 – 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Mitochondria 0.12 0 0.23 0.17 0.05 0.14 – 0.13 0.27 0.24 0.29 0.31 0 0 0 0 0 0 0.05 0 0 0 0 0
Nucleus 0.06 0.05 0.05 0 0.1 0.06 – 0.05 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Nuclear Periphery 0 0 0 0 0 0 – 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Nucleolus 0 0 0 0 0 0 – 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0.06 0
Peroxisomes 0 0 0 0 0 0 – 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
SpindlePole 0 0 0 0 0 0 – 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Vac/Vac Membrane 0.56 0.51 0.3 0.31 0.38 0.27 – 0.16 0.16 0.1 0.11 0.08 0.11 0.05 0 0 0 0.05 0.73 0.7 0.77 0.61 0.7 0.69
ensLOC DeepLoc
Localization WT1 WT2 WT3 RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3 WT1 WT2 WT3 AF100 AF140 AF180
Cortical Patches 2 0 0 0 0 0 – 2 0 0 0 0 0 1 0 0 0 0 1 3 1 0 0 0
Bud 0 0 5 1 1 5 – 14 3 16 7 8 0 0 0 0 0 0 11 7 5 1 2 3
Bud Neck 0 0 0 0 0 0 – 2 0 2 0 1 0 0 0 0 0 0 2 0 1 0 0 0
Bud Site 1 0 0 0 0 0 – 0 0 0 0 4 0 0 0 0 0 0 – – – – – –
Cell Periphery 0 0 0 0 0 0 – 1 0 2 0 2 0 0 1 1 1 0 0 0 0 0 0 0
Cytoplasm 129 123 197 229 232 376 – 492 389 483 330 315 208 279 359 136 120 132 38 28 34 0 0 1
Endoplasmic Reticulum 0 2 2 4 0 0 – 1 0 0 0 0 1 1 1 8 7 12 2 0 5 0 1 0
Endosome 23 3 2 0 4 2 – 2 1 2 0 0 1 3 1 1 1 2 13 10 15 41 23 45
Golgi 1 1 0 0 0 0 – 0 0 0 0 0 0 1 0 1 1 1 1 4 1 0 3 6
Mitochondria 46 4 78 54 17 74 – 85 131 143 123 131 0 1 1 4 2 4 22 4 10 0 2 5
Nucleus 24 11 18 14 38 31 – 31 12 8 7 2 5 1 1 1 2 4 4 0 1 1 6 6
Nuclear Periphery 5 0 0 1 0 2 – 9 4 0 1 2 2 0 0 0 0 0 0 0 0 2 2 10
Nucleolus 3 1 1 1 0 0 – 2 0 2 0 0 0 0 1 0 0 0 0 0 0 6 11 8
Peroxisomes 0 0 0 0 0 0 – 1 0 0 1 0 0 0 0 0 0 0 0 0 0 0 0 0
SpindlePole 2 0 3 0 0 0 – 6 1 4 3 6 0 0 0 0 0 1 6 3 3 3 2 0
Vac/Vac Membrane 210 111 100 99 139 138 – 101 77 60 46 33 26 15 7 3 3 7 309 161 293 92 133 201
Unique Cell Count 378 219 332 316 367 517 641 491 607 417 416 231 295 367 143 128 145 421 230 380 152 190 294
Labelled Cell Count 446 256 406 403 431 628 749 618 722 518 504 243 302 372 155 137 163 421 230 380 152 190 294


Vacuole

(Huh et al. Nature 2003 425:686)
Screen WT1 WT2 WT3 RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3 AF100 AF140 AF180
Mean Cell GFP Intensity (1e-4) 6.2 5.7 3.8 3.9 5.2 4.1 – 4.0 3.3 3.5 3.3 3.1 4.7 4.8 4.5 5.7 5.6 6.0 11.3 12.8 13.7
Std Deviation (1e-4) 4.2 1.4 1.0 1.3 2.4 2.3 – 1.5 1.3 1.3 1.0 1.1 1.1 1.2 1.1 1.8 1.6 2.6 3.2 4.8 4.5
Intensity Change (Log2) – – – 0.02 0.44 0.09 – 0.05 -0.23 -0.14 -0.23 -0.33 0.28 0.32 0.22 0.57 0.55 0.65 1.56 1.74 1.84


Localization RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3
Cortical Patches 0 0 0 – 0 0 0 0 0 0 0 0 0 0 0
Bud 0 0 0 – 0 0 0 0 0 0 0 0 0 0 0
Bud Neck 0 0 0 – 0 0 0 0 0 0 0 0 0 0 0
Bud Site 0 0 0 – 0 0 0 0 0 0 0 0 0 0 0
Cell Periphery 0 0 0 – 0 0 0 0 0 0 0 0 0 0 0
Cytoplasm 3.5 1.1 4.1 – 5.7 6.2 6.6 5.9 4.8 8.0 10.3 12.6 7.8 7.1 6.9
Endoplasmic Reticulum 0 0 0 – 0 0 0 0 0 0 0 0 0 0 0
Endosome 0 0 0 – 0 0 0 0 0 0 0 0 0 0 0
Golgi 0 0 0 – 0 0 0 0 0 0 0 0 0 0 0
Mitochondria 0 -7.3 0 – 0 0 0 0 0 0 0 0 -5.5 -5.6 -5.5
Nucleus -0.6 2.4 0.4 – -0.4 -2.2 -3.7 -2.8 -4.2 -1.9 -3.7 -4.2 -2.4 -1.8 -1.3
Nuclear Periphery 0 0 0 – 0 0 0 0 0 0 0 0 0 0 0
Nucleolus 0 0 0 – 0 0 0 0 0 0 0 0 0 0 0
Peroxisomes 0 0 0 – 0 0 0 0 0 0 0 0 0 0 0
SpindlePole 0 0 0 – 0 0 0 0 0 0 0 0 0 0 0
Vacuole 0.3 2.2 -1.1 – -5.2 -4.9 -7.9 -6.6 -7.9 -5.3 -8.1 -10.3 -6.8 -6.4 -6.1

Images

Circle plot not available

Protein Concentration and Protein Localization Data

R1 R2 R3
G1 Pre-START G1 Post-START S/G2 Metaphase Anaphase Telophase G1 Pre-START G1 Post-START S/G2 Metaphase Anaphase Telophase G1 Pre-START G1 Post-START S/G2 Metaphase Anaphase Telophase
Concentration 1.5487 2.2183 1.1405 0.3846 0.5465 1.5982 1.4515 1.6368 1.0704 1.4138 0.7969 1.0238 4.5865 4.3088 3.589 2.5038 3.2608 3.897
Actin 0.0029 0.0001 0.0047 0.0002 0.0252 0.0001 0.0127 0.0002 0.0141 0.0087 0.0113 0.0015 0.0173 0 0.0013 0 0.0026 0.0005
Bud 0.002 0.0066 0.0014 0.0019 0.0048 0.0012 0.0008 0.0041 0.0072 0.0019 0.0131 0.002 0.002 0.0044 0.0053 0.0012 0.0077 0.0023
Bud Neck 0.0019 0.0016 0.001 0.0017 0.0014 0.003 0.0044 0.0022 0.0026 0.0077 0.0068 0.0034 0.0031 0.0016 0.0031 0.0018 0.0023 0.0034
Bud Periphery 0.0014 0.0075 0.004 0.004 0.0082 0.0019 0.0017 0.0093 0.0109 0.004 0.0163 0.0052 0.0032 0.0084 0.0115 0.0035 0.0277 0.0071
Bud Site 0.0062 0.0112 0.0019 0.0062 0.0016 0.0007 0.0025 0.0188 0.0096 0.0363 0.0117 0.0015 0.0082 0.0105 0.0093 0.002 0.0049 0.0016
Cell Periphery 0.0011 0.0023 0.0016 0.0013 0.0013 0.0015 0.0018 0.0025 0.0027 0.003 0.0041 0.0022 0.0022 0.0035 0.0045 0.0016 0.0025 0.0021
Cytoplasm 0.0597 0.0601 0.0613 0.0989 0.0809 0.0654 0.0378 0.0524 0.0304 0.023 0.0234 0.0611 0.0426 0.0664 0.0254 0.0945 0.0175 0.0499
Cytoplasmic Foci 0.049 0.0381 0.0445 0.0681 0.0923 0.0501 0.0552 0.0363 0.0349 0.0222 0.0418 0.0515 0.042 0.0295 0.0308 0.0209 0.0147 0.0314
Eisosomes 0.0001 0 0.0002 0 0.0002 0 0.0001 0.0001 0.0001 0.0001 0.0001 0 0.0001 0 0 0 0.0001 0
Endoplasmic Reticulum 0.0063 0.009 0.0047 0.0045 0.0048 0.0065 0.0116 0.0048 0.0051 0.0058 0.0033 0.012 0.0088 0.0072 0.0057 0.0024 0.0054 0.0066
Endosome 0.3007 0.1854 0.2204 0.2282 0.1769 0.2304 0.3079 0.2038 0.26 0.1667 0.2185 0.2405 0.258 0.1503 0.2128 0.1323 0.1906 0.2162
Golgi 0.0101 0.0038 0.0048 0.0033 0.0054 0.0041 0.0158 0.0057 0.0238 0.0167 0.0226 0.009 0.008 0.0034 0.0102 0.0037 0.0064 0.0047
Lipid Particles 0.0354 0.015 0.0234 0.0119 0.0071 0.0128 0.0497 0.0213 0.0336 0.0128 0.0214 0.0169 0.0228 0.0172 0.0113 0.0175 0.011 0.019
Mitochondria 0.0151 0.0065 0.0073 0.0043 0.0044 0.0091 0.015 0.0151 0.0227 0.0107 0.0103 0.0086 0.0055 0.0043 0.0104 0.0091 0.0102 0.005
None 0.1439 0.1579 0.1759 0.0826 0.1629 0.085 0.0585 0.1134 0.0741 0.0506 0.0631 0.0944 0.0658 0.0836 0.0728 0.0852 0.0364 0.0435
Nuclear Periphery 0.0058 0.0153 0.0049 0.0034 0.0125 0.0082 0.0143 0.0131 0.0092 0.0089 0.006 0.0122 0.0291 0.0111 0.0099 0.0052 0.0349 0.0143
Nucleolus 0.0049 0.0088 0.006 0.0091 0.0045 0.0044 0.0091 0.0116 0.0175 0.0108 0.0258 0.0075 0.0059 0.0107 0.0181 0.0049 0.0036 0.0103
Nucleus 0.009 0.0133 0.0056 0.0141 0.0082 0.01 0.0093 0.0127 0.0097 0.0105 0.046 0.0111 0.0169 0.0164 0.0168 0.0054 0.011 0.0178
Peroxisomes 0.0045 0.002 0.0041 0.0027 0.0016 0.0011 0.012 0.0033 0.0077 0.0044 0.0104 0.0038 0.0026 0.0015 0.0038 0.0011 0.0021 0.0041
Punctate Nuclear 0.0055 0.0017 0.0017 0.0041 0.0062 0.0019 0.008 0.0025 0.0029 0.0018 0.0139 0.0018 0.0051 0.0025 0.0039 0.0006 0.0031 0.002
Vacuole 0.295 0.3946 0.3547 0.3874 0.342 0.4284 0.3247 0.4002 0.3362 0.5068 0.3684 0.381 0.383 0.4978 0.4583 0.5088 0.4675 0.4853
Vacuole Periphery 0.0396 0.0595 0.0659 0.0622 0.0475 0.0742 0.0471 0.0667 0.085 0.0866 0.0616 0.073 0.0676 0.0697 0.0744 0.0983 0.138 0.0727

Sequencing Data

R1 R2
G1 Post-START S/G2 Metaphase Anaphase Telophase G1 Post-START S/G2 Metaphase Anaphase Telophase
Gene Expression 91.9504 26.1539 22.1798 70.7811 70.4468 50.127 18.8887 21.6726 35.9352 58.4403
Translational Efficiency 1.0651 0.7319 1.1413 0.7005 1.653 2.0688 1.054 1.127 1.7323 1.5818

Hit Data

Dataset Hit
Protein Concentration ✔
Protein Localization ✘
Gene Expression ✔
Translational Efficiency ✘
Standard name
Human Ortholog
Description Adhesion subunit of a-agglutinin of a-cells; C-terminal sequence acts as a ligand for alpha-agglutinin (Sag1p) during agglutination, modified with O-linked oligomannosyl chains, linked to anchorage subunit Aga1p via two disulfide bonds
Localization
Cell Percentages vacuole (27%), cytoplasm (4%), mixed (42%)
Cell Cycle Regulation No
Subcompartmental Group N/A

Aga2

Aga2


Nearest neighbours ( genes)

correlation threshold

0.6

1.0

0.80


Biological Process (Gene Ontology)
Molecular Function (Gene Ontology)
Cellular Component (Gene Ontology)

Endocytosis
Temp Actin Patch (Sac6-tdTomato) Cortical Patch (Sla1-GFP) Late Endosome (Snf7-GFP) Vacuole (Vph1-GFP)
37℃
RT
Cell Cycle Omics

CYCLoPs (Aga2-GFP)
Gene / Allele Actin Patch (Sac6-tdTomato) Cortical Patch (Sla1-GFP) Late Endosome (Snf7-GFP) Vacuole (Sac6-tdTomato)
Gene Images
Gene Images
Images are not yet available
Images are not yet available