Standard name
Description Adhesion subunit of a-agglutinin of a-cells; C-terminal sequence acts as a ligand for alpha-agglutinin (Sag1p) during agglutination, modified with O-linked oligomannosyl chains, linked to anchorage subunit Aga1p via two disulfide bonds

Micrographs

ensLOC DeepLoc
Localization WT1 WT2 WT3 RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3 WT1 WT2 WT3 AF100 AF140 AF180
Cortical Patches 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Bud 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Bud Neck 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Bud Site 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Cell Periphery 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Cytoplasm 0.34 0.56 0.59 0.72 0.63 0.73 0.77 0.79 0.8 0.79 0.76 0.9 0.95 0.98 0.95 0.94 0.91 0.09 0.12 0.09 0 0 0
Endoplasmic Reticulum 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0.06 0.05 0.08 0 0 0 0 0 0
Endosome 0.06 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0.05 0 0.27 0.12 0.15
Golgi 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Mitochondria 0.12 0 0.23 0.17 0.05 0.14 0.13 0.27 0.24 0.29 0.31 0 0 0 0 0 0 0.05 0 0 0 0 0
Nucleus 0.06 0.05 0.05 0 0.1 0.06 0.05 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Nuclear Periphery 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Nucleolus 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0.06 0
Peroxisomes 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
SpindlePole 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Vac/Vac Membrane 0.56 0.51 0.3 0.31 0.38 0.27 0.16 0.16 0.1 0.11 0.08 0.11 0.05 0 0 0 0.05 0.73 0.7 0.77 0.61 0.7 0.69
ensLOC DeepLoc
Localization WT1 WT2 WT3 RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3 WT1 WT2 WT3 AF100 AF140 AF180
Cortical Patches 2 0 0 0 0 0 2 0 0 0 0 0 1 0 0 0 0 1 3 1 0 0 0
Bud 0 0 5 1 1 5 14 3 16 7 8 0 0 0 0 0 0 11 7 5 1 2 3
Bud Neck 0 0 0 0 0 0 2 0 2 0 1 0 0 0 0 0 0 2 0 1 0 0 0
Bud Site 1 0 0 0 0 0 0 0 0 0 4 0 0 0 0 0 0
Cell Periphery 0 0 0 0 0 0 1 0 2 0 2 0 0 1 1 1 0 0 0 0 0 0 0
Cytoplasm 129 123 197 229 232 376 492 389 483 330 315 208 279 359 136 120 132 38 28 34 0 0 1
Endoplasmic Reticulum 0 2 2 4 0 0 1 0 0 0 0 1 1 1 8 7 12 2 0 5 0 1 0
Endosome 23 3 2 0 4 2 2 1 2 0 0 1 3 1 1 1 2 13 10 15 41 23 45
Golgi 1 1 0 0 0 0 0 0 0 0 0 0 1 0 1 1 1 1 4 1 0 3 6
Mitochondria 46 4 78 54 17 74 85 131 143 123 131 0 1 1 4 2 4 22 4 10 0 2 5
Nucleus 24 11 18 14 38 31 31 12 8 7 2 5 1 1 1 2 4 4 0 1 1 6 6
Nuclear Periphery 5 0 0 1 0 2 9 4 0 1 2 2 0 0 0 0 0 0 0 0 2 2 10
Nucleolus 3 1 1 1 0 0 2 0 2 0 0 0 0 1 0 0 0 0 0 0 6 11 8
Peroxisomes 0 0 0 0 0 0 1 0 0 1 0 0 0 0 0 0 0 0 0 0 0 0 0
SpindlePole 2 0 3 0 0 0 6 1 4 3 6 0 0 0 0 0 1 6 3 3 3 2 0
Vac/Vac Membrane 210 111 100 99 139 138 101 77 60 46 33 26 15 7 3 3 7 309 161 293 92 133 201
Unique Cell Count 378 219 332 316 367 517 641 491 607 417 416 231 295 367 143 128 145 421 230 380 152 190 294
Labelled Cell Count 446 256 406 403 431 628 749 618 722 518 504 243 302 372 155 137 163 421 230 380 152 190 294


Vacuole

(Huh et al. Nature 2003 425:686)
Screen WT1 WT2 WT3 RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3 AF100 AF140 AF180
Mean Cell GFP Intensity (1e-4) 6.2 5.7 3.8 3.9 5.2 4.1 4.0 3.3 3.5 3.3 3.1 4.7 4.8 4.5 5.7 5.6 6.0 11.3 12.8 13.7
Std Deviation (1e-4) 4.2 1.4 1.0 1.3 2.4 2.3 1.5 1.3 1.3 1.0 1.1 1.1 1.2 1.1 1.8 1.6 2.6 3.2 4.8 4.5
Intensity Change (Log2) 0.02 0.44 0.09 0.05 -0.23 -0.14 -0.23 -0.33 0.28 0.32 0.22 0.57 0.55 0.65 1.56 1.74 1.84


Localization RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3
Cortical Patches 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Bud 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Bud Neck 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Bud Site 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Cell Periphery 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Cytoplasm 3.5 1.1 4.1 5.7 6.2 6.6 5.9 4.8 8.0 10.3 12.6 7.8 7.1 6.9
Endoplasmic Reticulum 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Endosome 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Golgi 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Mitochondria 0 -7.3 0 0 0 0 0 0 0 0 0 -5.5 -5.6 -5.5
Nucleus -0.6 2.4 0.4 -0.4 -2.2 -3.7 -2.8 -4.2 -1.9 -3.7 -4.2 -2.4 -1.8 -1.3
Nuclear Periphery 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Nucleolus 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Peroxisomes 0 0 0 0 0 0 0 0 0 0 0 0 0 0
SpindlePole 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Vacuole 0.3 2.2 -1.1 -5.2 -4.9 -7.9 -6.6 -7.9 -5.3 -8.1 -10.3 -6.8 -6.4 -6.1


Endocytosis
Temp Actin Patch (Sac6-tdTomato) Cortical Patch (Sla1-GFP) Late Endosome (Snf7-GFP) Vacuole (Vph1-GFP)
37℃
RT
Cell Cycle Omics

CYCLoPs (Aga2-GFP)
Gene / Allele Actin Patch (Sac6-tdTomato) Cortical Patch (Sla1-GFP) Late Endosome (Snf7-GFP) Vacuole (Sac6-tdTomato)
Gene Images
Gene Images
Images are not yet available
Images are not yet available