Standard name | |
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Description | C-24(28) sterol reductase; catalyzes the final step in ergosterol biosynthesis; mutants are viable, but lack ergosterol |
ensLOC | DeepLoc | |||||||||||||||||||||||
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Localization | WT1 | WT2 | WT3 | RAP60 | RAP140 | RAP220 | RAP300 | RAP380 | RAP460 | RAP540 | RAP620 | RAP700 | HU80 | HU120 | HU160 | rpd3Δ_1 | rpd3Δ_2 | rpd3Δ_3 | WT1 | WT2 | WT3 | AF100 | AF140 | AF180 |
Cortical Patches | – | 6 | 9 | 13 | 4 | 8 | 9 | 14 | – | 9 | 6 | 5 | 1 | 1 | 1 | – | – | – | 0 | 0 | 4 | 1 | 2 | 0 |
Bud | – | 1 | 1 | 0 | 0 | 1 | 1 | 0 | – | 0 | 0 | 0 | 0 | 0 | 0 | – | – | – | 0 | 1 | 1 | 0 | 0 | 0 |
Bud Neck | – | 0 | 0 | 0 | 0 | 0 | 0 | 0 | – | 0 | 0 | 0 | 0 | 0 | 0 | – | – | – | 0 | 0 | 0 | 0 | 0 | 0 |
Bud Site | – | 0 | 0 | 0 | 0 | 0 | 0 | 0 | – | 0 | 0 | 0 | 0 | 0 | 0 | – | – | – | – | – | – | – | – | – |
Cell Periphery | – | 9 | 34 | 19 | 14 | 11 | 9 | 9 | – | 12 | 3 | 8 | 7 | 7 | 8 | – | – | – | 0 | 0 | 7 | 0 | 0 | 0 |
Cytoplasm | – | 25 | 14 | 12 | 12 | 12 | 15 | 27 | – | 18 | 3 | 12 | 2 | 2 | 6 | – | – | – | 0 | 5 | 0 | 0 | 0 | 0 |
Endoplasmic Reticulum | – | 116 | 129 | 74 | 67 | 99 | 80 | 88 | – | 50 | 23 | 42 | 14 | 14 | 20 | – | – | – | 17 | 141 | 145 | 19 | 72 | 87 |
Endosome | – | 0 | 1 | 0 | 2 | 0 | 0 | 1 | – | 1 | 0 | 0 | 0 | 0 | 0 | – | – | – | 0 | 1 | 3 | 0 | 0 | 0 |
Golgi | – | 1 | 4 | 6 | 6 | 3 | 2 | 0 | – | 0 | 0 | 0 | 0 | 0 | 0 | – | – | – | 0 | 2 | 1 | 0 | 3 | 0 |
Mitochondria | – | 6 | 38 | 37 | 17 | 37 | 33 | 45 | – | 52 | 27 | 47 | 0 | 0 | 1 | – | – | – | 0 | 3 | 7 | 1 | 1 | 2 |
Nucleus | – | 0 | 1 | 0 | 0 | 0 | 0 | 1 | – | 0 | 0 | 0 | 0 | 0 | 0 | – | – | – | 0 | 0 | 0 | 0 | 0 | 0 |
Nuclear Periphery | – | 2 | 13 | 7 | 4 | 2 | 6 | 12 | – | 2 | 1 | 8 | 0 | 0 | 0 | – | – | – | 0 | 1 | 2 | 0 | 2 | 0 |
Nucleolus | – | 0 | 0 | 0 | 0 | 1 | 0 | 0 | – | 0 | 0 | 0 | 0 | 0 | 0 | – | – | – | 0 | 0 | 0 | 0 | 0 | 0 |
Peroxisomes | – | 0 | 0 | 0 | 1 | 0 | 0 | 0 | – | 0 | 0 | 0 | 0 | 0 | 0 | – | – | – | 0 | 0 | 0 | 0 | 0 | 0 |
SpindlePole | – | 0 | 0 | 0 | 0 | 0 | 0 | 0 | – | 0 | 0 | 0 | 0 | 0 | 0 | – | – | – | 0 | 0 | 0 | 0 | 0 | 0 |
Vac/Vac Membrane | – | 4 | 14 | 4 | 10 | 11 | 3 | 13 | – | 3 | 4 | 1 | 4 | 5 | 0 | – | – | – | 0 | 5 | 2 | 2 | 2 | 0 |
Unique Cell Count | 148 | 171 | 113 | 107 | 141 | 126 | 165 | 111 | 53 | 95 | 22 | 23 | 30 | 18 | 165 | 180 | 29 | 87 | 92 | |||||
Labelled Cell Count | 170 | 258 | 172 | 137 | 185 | 158 | 210 | 147 | 67 | 123 | 28 | 29 | 36 | 18 | 165 | 180 | 29 | 87 | 92 |
Screen | WT1 | WT2 | WT3 | RAP60 | RAP140 | RAP220 | RAP300 | RAP380 | RAP460 | RAP540 | RAP620 | RAP700 | HU80 | HU120 | HU160 | rpd3Δ_1 | rpd3Δ_2 | rpd3Δ_3 | AF100 | AF140 | AF180 |
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Mean Cell GFP Intensity (1e-4) | – | 11.5 | 10.1 | 11.1 | 12.1 | 10.9 | 10.8 | 10.6 | – | 9.7 | 10.0 | 11.7 | 14.0 | 14.0 | 13.2 | – | – | – | – | 12.9 | 13.3 |
Std Deviation (1e-4) | – | 2.2 | 2.5 | 2.3 | 2.5 | 1.9 | 2.3 | 2.1 | – | 2.2 | 2.3 | 2.7 | 2.0 | 2.0 | 2.1 | – | – | – | – | 3.4 | 2.5 |
Intensity Change (Log2) | – | – | – | 0.13 | 0.26 | 0.1 | 0.09 | 0.06 | – | -0.07 | -0.02 | 0.21 | 0.47 | 0.47 | 0.38 | – | – | – | – | 0.35 | 0.39 |
Localization | RAP60 | RAP140 | RAP220 | RAP300 | RAP380 | RAP460 | RAP540 | RAP620 | RAP700 | HU80 | HU120 | HU160 | rpd3Δ_1 | rpd3Δ_2 | rpd3Δ_3 |
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Cortical Patches | 1.9 | -0.6 | 0.2 | 0.7 | 1.2 | – | 1.0 | 0 | 0 | 0 | 0 | 0 | – | – | – |
Bud | 0 | 0 | 0 | 0 | 0 | – | 0 | 0 | 0 | 0 | 0 | 0 | – | – | – |
Bud Neck | 0 | 0 | 0 | 0 | 0 | – | 0 | 0 | 0 | 0 | 0 | 0 | – | – | – |
Bud Site | 0 | 0 | 0 | 0 | 0 | – | 0 | 0 | 0 | 0 | 0 | 0 | – | – | – |
Cell Periphery | -0.6 | -1.5 | -3.0 | -3.1 | -4.0 | – | -2.0 | -2.4 | -2.5 | 0 | 0 | 0.8 | – | – | – |
Cytoplasm | 0.7 | 0.8 | 0.1 | 1.1 | 2.3 | – | 2.1 | 0 | 1.2 | 0 | 0 | 0 | – | – | – |
Endoplasmic Reticulum | -1.8 | -2.3 | -1.0 | -2.2 | -4.2 | – | -5.2 | -4.4 | -5.1 | -1.2 | -1.5 | -1.0 | – | – | – |
Endosome | 0 | 0 | 0 | 0 | 0 | – | 0 | 0 | 0 | 0 | 0 | 0 | – | – | – |
Golgi | 0 | 0 | 0 | 0 | 0 | – | 0 | 0 | 0 | 0 | 0 | 0 | – | – | – |
Mitochondria | 2.0 | -1.3 | 0.8 | 0.8 | 1.1 | – | 4.3 | 4.0 | 4.6 | 0 | 0 | -2.4 | – | – | – |
Nucleus | 0 | 0 | 0 | 0 | 0 | – | 0 | 0 | 0 | 0 | 0 | 0 | – | – | – |
Nuclear Periphery | -0.5 | -1.3 | -2.5 | -1.0 | -0.1 | – | -2.1 | 0 | 0.2 | 0 | 0 | 0 | – | – | – |
Nucleolus | 0 | 0 | 0 | 0 | 0 | – | 0 | 0 | 0 | 0 | 0 | 0 | – | – | – |
Peroxisomes | 0 | 0 | 0 | 0 | 0 | – | 0 | 0 | 0 | 0 | 0 | 0 | – | – | – |
SpindlePole | 0 | 0 | 0 | 0 | 0 | – | 0 | 0 | 0 | 0 | 0 | 0 | – | – | – |
Vacuole | -1.6 | 0.3 | -0.1 | -2.1 | -0.1 | – | -1.9 | 0 | -2.4 | 0 | 0 | 0 | – | – | – |
Temp | Actin Patch (Sac6-tdTomato) | Cortical Patch (Sla1-GFP) | Late Endosome (Snf7-GFP) | Vacuole (Vph1-GFP) |
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37℃ | ||||
RT |
Gene / Allele | Actin Patch (Sac6-tdTomato) | Cortical Patch (Sla1-GFP) | Late Endosome (Snf7-GFP) | Vacuole (Sac6-tdTomato) |
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Gene | Images |
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Gene | Images |
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