Standard name
Description C-24(28) sterol reductase; catalyzes the final step in ergosterol biosynthesis; mutants are viable, but lack ergosterol
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Micrographs

ensLOC DeepLoc
Localization WT1 WT2 WT3 RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3 WT1 WT2 WT3 AF100 AF140 AF180
Cortical Patches – 0 0.05 0.12 0 0.06 0.07 0.08 – 0.08 0.11 0.05 0.05 0 0 – – – 0 0 0 0 0 0
Bud – 0 0 0 0 0 0 0 – 0 0 0 0 0 0 – – – 0.05 0 0 0 0 0
Bud Neck – 0 0 0 0 0 0 0 – 0 0 0 0 0 0 – – – 0 0 0 0 0 0
Bud Site – 0 0 0 0 0 0 0 – 0 0 0 0 0 0 – – – – – – – – –
Cell Periphery – 0.06 0.2 0.17 0.13 0.08 0.07 0.05 – 0.11 0.06 0.08 0.32 0.3 0.27 – – – 0 0 0 0 0 0
Cytoplasm – 0.17 0.08 0.11 0.11 0.09 0.12 0.16 – 0.16 0.06 0.13 0.09 0.09 0.2 – – – 0 0 0 0 0 0
Endoplasmic Reticulum – 0.78 0.75 0.65 0.63 0.7 0.63 0.53 – 0.45 0.43 0.44 0.64 0.61 0.67 – – – 0.94 0.85 0.81 0.66 0.83 0.95
Endosome – 0 0 0 0 0 0 0 – 0 0 0 0 0 0 – – – 0 0 0 0 0 0
Golgi – 0 0 0.05 0.06 0 0 0 – 0 0 0 0 0 0 – – – 0 0 0 0 0 0
Mitochondria – 0 0.22 0.33 0.16 0.26 0.26 0.27 – 0.47 0.51 0.49 0 0 0 – – – 0 0 0 0.07 0 0
Nucleus – 0 0 0 0 0 0 0 – 0 0 0 0 0 0 – – – 0 0 0 0 0 0
Nuclear Periphery – 0 0.08 0.06 0 0 0.05 0.07 – 0 0 0.08 0 0 0 – – – 0 0 0 0 0 0
Nucleolus – 0 0 0 0 0 0 0 – 0 0 0 0 0 0 – – – 0 0 0 0 0 0
Peroxisomes – 0 0 0 0 0 0 0 – 0 0 0 0 0 0 – – – 0 0 0 0 0 0
SpindlePole – 0 0 0 0 0 0 0 – 0 0 0 0 0 0 – – – 0 0 0 0 0 0
Vac/Vac Membrane – 0 0.08 0 0.09 0.08 0 0.08 – 0 0.08 0 0.18 0.22 0 – – – 0 0 0 0.09 0 0
ensLOC DeepLoc
Localization WT1 WT2 WT3 RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3 WT1 WT2 WT3 AF100 AF140 AF180
Cortical Patches – 6 9 13 4 8 9 14 – 9 6 5 1 1 1 – – – 0 0 4 1 2 0
Bud – 1 1 0 0 1 1 0 – 0 0 0 0 0 0 – – – 0 1 1 0 0 0
Bud Neck – 0 0 0 0 0 0 0 – 0 0 0 0 0 0 – – – 0 0 0 0 0 0
Bud Site – 0 0 0 0 0 0 0 – 0 0 0 0 0 0 – – – – – – – – –
Cell Periphery – 9 34 19 14 11 9 9 – 12 3 8 7 7 8 – – – 0 0 7 0 0 0
Cytoplasm – 25 14 12 12 12 15 27 – 18 3 12 2 2 6 – – – 0 5 0 0 0 0
Endoplasmic Reticulum – 116 129 74 67 99 80 88 – 50 23 42 14 14 20 – – – 17 141 145 19 72 87
Endosome – 0 1 0 2 0 0 1 – 1 0 0 0 0 0 – – – 0 1 3 0 0 0
Golgi – 1 4 6 6 3 2 0 – 0 0 0 0 0 0 – – – 0 2 1 0 3 0
Mitochondria – 6 38 37 17 37 33 45 – 52 27 47 0 0 1 – – – 0 3 7 1 1 2
Nucleus – 0 1 0 0 0 0 1 – 0 0 0 0 0 0 – – – 0 0 0 0 0 0
Nuclear Periphery – 2 13 7 4 2 6 12 – 2 1 8 0 0 0 – – – 0 1 2 0 2 0
Nucleolus – 0 0 0 0 1 0 0 – 0 0 0 0 0 0 – – – 0 0 0 0 0 0
Peroxisomes – 0 0 0 1 0 0 0 – 0 0 0 0 0 0 – – – 0 0 0 0 0 0
SpindlePole – 0 0 0 0 0 0 0 – 0 0 0 0 0 0 – – – 0 0 0 0 0 0
Vac/Vac Membrane – 4 14 4 10 11 3 13 – 3 4 1 4 5 0 – – – 0 5 2 2 2 0
Unique Cell Count 148 171 113 107 141 126 165 111 53 95 22 23 30 18 165 180 29 87 92
Labelled Cell Count 170 258 172 137 185 158 210 147 67 123 28 29 36 18 165 180 29 87 92


Endoplasmic Reticulum

(Huh et al. Nature 2003 425:686)
Screen WT1 WT2 WT3 RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3 AF100 AF140 AF180
Mean Cell GFP Intensity (1e-4) – 11.5 10.1 11.1 12.1 10.9 10.8 10.6 – 9.7 10.0 11.7 14.0 14.0 13.2 – – – – 12.9 13.3
Std Deviation (1e-4) – 2.2 2.5 2.3 2.5 1.9 2.3 2.1 – 2.2 2.3 2.7 2.0 2.0 2.1 – – – – 3.4 2.5
Intensity Change (Log2) – – – 0.13 0.26 0.1 0.09 0.06 – -0.07 -0.02 0.21 0.47 0.47 0.38 – – – – 0.35 0.39


Localization RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3
Cortical Patches 1.9 -0.6 0.2 0.7 1.2 – 1.0 0 0 0 0 0 – – –
Bud 0 0 0 0 0 – 0 0 0 0 0 0 – – –
Bud Neck 0 0 0 0 0 – 0 0 0 0 0 0 – – –
Bud Site 0 0 0 0 0 – 0 0 0 0 0 0 – – –
Cell Periphery -0.6 -1.5 -3.0 -3.1 -4.0 – -2.0 -2.4 -2.5 0 0 0.8 – – –
Cytoplasm 0.7 0.8 0.1 1.1 2.3 – 2.1 0 1.2 0 0 0 – – –
Endoplasmic Reticulum -1.8 -2.3 -1.0 -2.2 -4.2 – -5.2 -4.4 -5.1 -1.2 -1.5 -1.0 – – –
Endosome 0 0 0 0 0 – 0 0 0 0 0 0 – – –
Golgi 0 0 0 0 0 – 0 0 0 0 0 0 – – –
Mitochondria 2.0 -1.3 0.8 0.8 1.1 – 4.3 4.0 4.6 0 0 -2.4 – – –
Nucleus 0 0 0 0 0 – 0 0 0 0 0 0 – – –
Nuclear Periphery -0.5 -1.3 -2.5 -1.0 -0.1 – -2.1 0 0.2 0 0 0 – – –
Nucleolus 0 0 0 0 0 – 0 0 0 0 0 0 – – –
Peroxisomes 0 0 0 0 0 – 0 0 0 0 0 0 – – –
SpindlePole 0 0 0 0 0 – 0 0 0 0 0 0 – – –
Vacuole -1.6 0.3 -0.1 -2.1 -0.1 – -1.9 0 -2.4 0 0 0 – – –


Endocytosis
Temp Actin Patch (Sac6-tdTomato) Cortical Patch (Sla1-GFP) Late Endosome (Snf7-GFP) Vacuole (Vph1-GFP)
37℃
RT
Cell Cycle Omics

CYCLoPs (Erg4-GFP)
Gene / Allele Actin Patch (Sac6-tdTomato) Cortical Patch (Sla1-GFP) Late Endosome (Snf7-GFP) Vacuole (Sac6-tdTomato)
Gene Images
Gene Images
Images are not yet available
Images are not yet available