Standard name
Description tRNAHis guanylyltransferase; adds a guanosine residue to the 5' end of tRNAH is after transcription and RNase P cleavage; can also catalyze reverse (3'-5') polymerization with certain substrates in a template-dependent reaction; couples nuclear division and migration to cell budding and cytokinesis; essential enzyme conserved among eukaryotes

Micrographs

ensLOC DeepLoc
Localization WT1 WT2 WT3 RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3 WT1 WT2 WT3 AF100 AF140 AF180
Cortical Patches 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Bud 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Bud Neck 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Bud Site 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Cell Periphery 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Cytoplasm 0.85 0.99 0.97 0.98 0.95 0.84 0.83 0.76 0.75 0.7 0.69 0.99 0.99 0.98 0.93 0.9 0.93 0.8 0.91 0.91 0.94 0.89 0.79
Endoplasmic Reticulum 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0.05 0 0 0 0 0 0 0 0
Endosome 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Golgi 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Mitochondria 0.15 0 0 0 0 0.19 0.07 0.2 0.22 0.26 0.31 0 0 0 0 0 0 0 0 0 0 0 0
Nucleus 0 0 0 0 0 0.05 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Nuclear Periphery 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Nucleolus 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Peroxisomes 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
SpindlePole 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Vac/Vac Membrane 0 0 0 0 0.06 0.06 0.11 0.12 0.09 0.12 0.09 0 0 0 0.09 0.14 0.11 0 0 0 0 0 0
ensLOC DeepLoc
Localization WT1 WT2 WT3 RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3 WT1 WT2 WT3 AF100 AF140 AF180
Cortical Patches 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 1 2 2 0 0 3
Bud 0 1 0 0 0 1 0 1 2 0 2 1 0 0 0 0 0 2 5 1 1 1 7
Bud Neck 0 0 1 0 1 0 1 1 1 0 2 0 0 0 0 0 0 0 1 0 0 0 1
Bud Site 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Cell Periphery 0 1 4 1 3 0 4 3 2 3 3 2 0 5 7 6 2 0 0 0 0 0 1
Cytoplasm 41 382 256 166 222 122 210 132 172 97 143 187 167 209 150 143 143 67 347 241 122 169 158
Endoplasmic Reticulum 1 0 0 0 0 0 1 0 4 0 0 1 1 2 8 2 2 0 0 0 0 1 4
Endosome 0 0 0 0 0 0 1 0 1 1 2 0 0 1 1 1 1 2 2 3 1 2 8
Golgi 0 1 1 0 0 0 0 0 0 0 0 1 0 0 0 0 2 2 4 3 0 0 1
Mitochondria 7 2 1 1 4 28 18 35 51 36 64 0 0 0 3 1 1 2 3 6 0 1 2
Nucleus 0 3 1 2 1 7 6 2 4 5 4 0 0 1 1 0 0 0 2 0 0 0 0
Nuclear Periphery 2 0 0 0 1 0 0 0 0 0 1 0 0 1 0 0 0 0 0 0 0 1 1
Nucleolus 0 1 0 0 0 1 0 0 0 1 0 0 0 0 0 0 0 0 0 0 0 0 0
Peroxisomes 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 1 0 0 0
SpindlePole 0 0 0 0 0 0 2 0 1 0 3 0 0 0 0 0 0 0 2 0 0 2 2
Vac/Vac Membrane 2 4 8 3 13 8 28 21 20 16 19 1 1 2 15 22 17 0 6 0 1 3 3
Unique Cell Count 48 386 263 170 234 145 252 174 230 139 208 189 169 213 161 159 154 85 384 267 130 189 200
Labelled Cell Count 53 395 272 173 245 167 271 195 258 159 243 193 169 221 185 175 168 85 384 267 130 189 200


Cytoplasm, Nucleus

(Huh et al. Nature 2003 425:686)
Screen WT1 WT2 WT3 RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3 AF100 AF140 AF180
Mean Cell GFP Intensity (1e-4) 6.7 7.3 6.9 7.2 8.0 5.2 6.3 5.7 6.0 5.6 5.5 7.9 8.2 8.4 9.8 10.3 10.2 7.2 7.6 8.1
Std Deviation (1e-4) 1.8 1.9 1.6 1.9 2.3 1.5 1.5 2.0 1.6 1.6 1.2 2.1 2.1 2.3 2.4 2.5 2.5 1.9 2.2 2.5
Intensity Change (Log2) 0.05 0.21 -0.41 -0.14 -0.28 -0.2 -0.31 -0.32 0.19 0.25 0.28 0.5 0.58 0.56 0.06 0.14 0.22


Localization RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3
Cortical Patches 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Bud 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Bud Neck 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Bud Site 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Cell Periphery 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Cytoplasm 0.2 -1.4 -4.9 -5.4 -7.0 -7.4 -8.0 -8.6 1.2 1.0 0.6 -2.1 -3.2 -2.2
Endoplasmic Reticulum 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Endosome 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Golgi 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Mitochondria 0 0 7.1 4.1 7.3 7.9 8.4 9.5 0 0 0 0 0 0
Nucleus 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Nuclear Periphery 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Nucleolus 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Peroxisomes 0 0 0 0 0 0 0 0 0 0 0 0 0 0
SpindlePole 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Vacuole 0 1.4 1.2 3.6 3.7 2.7 3.4 2.8 0 0 0 2.8 4.2 3.3


Endocytosis
Temp Actin Patch (Sac6-tdTomato) Cortical Patch (Sla1-GFP) Late Endosome (Snf7-GFP) Vacuole (Vph1-GFP)
37℃
RT
Cell Cycle Omics

CYCLoPs (Thg1-GFP)
Gene / Allele Actin Patch (Sac6-tdTomato) Cortical Patch (Sla1-GFP) Late Endosome (Snf7-GFP) Vacuole (Sac6-tdTomato)
Gene Images
Gene Images
Images are not yet available
Images are not yet available