Standard name
Description Subunit of RNase MRP, nuclear RNase P and telomerase; forms a soluble heterodimer with Pop7p that binds P3 domain of RNase MRP and RNase P RNAs; RNase MRP cleaves pre-rRNA, nuclear RNase P cleaves tRNA precursors to generate mature 5' ends and facilitates turnover of nuclear RNAs, while telomerase replenishes telomeric DNA; relocalizes to the cytosol in response to hypoxia

Micrographs

ensLOC DeepLoc
Localization WT1 WT2 WT3 RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3 WT1 WT2 WT3 AF100 AF140 AF180
Cortical Patches 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Bud 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Bud Neck 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Bud Site 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Cell Periphery 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Cytoplasm 0 0.05 0 0 0 0 0 0 0 0 0 0.1 0.14 0.18 0.11 0.15 0.13 0 0 0 0 0 0
Endoplasmic Reticulum 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0.05 0 0 0 0 0 0 0 0
Endosome 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Golgi 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Mitochondria 0 0 0.21 0.2 0.05 0.31 0.27 0.54 0.34 0.52 0.42 0 0 0 0 0.05 0 0 0 0 0 0 0
Nucleus 0.96 0.92 0.94 0.9 0.88 0.82 0.82 0.72 0.77 0.76 0.79 0.87 0.8 0.71 0.66 0.67 0.7 0.92 0.86 0.9 0.74 0.6 0.48
Nuclear Periphery 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Nucleolus 0 0 0 0.05 0 0.05 0.09 0.09 0.07 0.1 0.09 0 0 0.06 0.05 0 0 0 0 0 0 0.06 0.12
Peroxisomes 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
SpindlePole 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Vac/Vac Membrane 0 0 0 0 0 0 0 0 0 0 0 0 0 0.06 0.21 0.21 0.19 0 0.06 0 0.14 0.23 0.3
ensLOC DeepLoc
Localization WT1 WT2 WT3 RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3 WT1 WT2 WT3 AF100 AF140 AF180
Cortical Patches 0 1 0 0 0 0 0 0 0 0 0 0 1 0 0 0 0 0 1 0 0 0 0
Bud 0 0 2 0 0 1 3 6 3 3 10 0 0 0 0 0 0 3 5 3 2 3 1
Bud Neck 0 0 0 0 0 0 0 0 0 1 0 0 0 0 0 0 0 0 1 0 0 0 0
Bud Site 0 0 0 0 2 6 16 11 11 9 24 0 0 0 0 0 0
Cell Periphery 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Cytoplasm 8 16 6 13 15 12 22 15 16 11 6 24 29 44 25 20 22 2 0 0 1 1 2
Endoplasmic Reticulum 0 0 0 0 0 0 1 0 0 0 0 0 0 0 11 5 5 0 0 0 0 0 2
Endosome 0 0 0 0 2 0 0 0 4 0 0 0 3 1 5 3 2 3 3 2 2 6 5
Golgi 0 1 0 0 0 0 0 0 0 0 0 0 0 0 1 0 1 2 5 0 0 0 1
Mitochondria 13 4 70 58 18 109 147 304 165 226 225 2 0 0 2 7 2 5 0 0 2 2 4
Nucleus 556 324 306 259 310 286 443 411 371 334 424 207 159 176 151 89 116 544 302 296 164 146 137
Nuclear Periphery 1 0 1 6 6 3 11 6 19 8 7 1 1 5 3 2 1 0 0 1 1 3 2
Nucleolus 2 1 8 15 13 19 49 51 33 44 49 5 9 14 11 5 3 10 6 8 8 15 34
Peroxisomes 0 0 0 0 0 0 0 0 0 0 0 0 0 3 0 0 0 0 0 0 0 0 0
SpindlePole 0 0 0 0 1 0 0 0 0 0 0 0 1 1 0 1 0 0 1 0 2 3 4
Vac/Vac Membrane 19 14 10 3 12 4 11 8 16 6 6 5 9 16 48 28 32 20 19 11 31 57 86
Unique Cell Count 579 351 327 289 353 350 543 568 479 437 534 238 200 249 228 133 166 595 352 328 222 244 285
Labelled Cell Count 599 361 403 354 379 440 703 812 638 642 751 244 212 260 257 160 184 595 352 328 222 244 285


Nucleus

(Huh et al. Nature 2003 425:686)
Screen WT1 WT2 WT3 RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3 AF100 AF140 AF180
Mean Cell GFP Intensity (1e-4) 7.8 9.8 5.6 6.5 6.4 5.0 4.9 4.1 4.9 4.4 4.4 8.7 8.8 8.4 10.4 9.0 9.9 8.7 9.1 8.8
Std Deviation (1e-4) 1.2 1.7 1.1 1.8 1.6 1.3 1.3 1.1 1.4 1.1 1.1 1.7 2.3 2.3 2.5 2.5 2.2 1.9 2.2 1.6
Intensity Change (Log2) 0.22 0.19 -0.16 -0.19 -0.45 -0.19 -0.36 -0.35 0.64 0.65 0.58 0.9 0.68 0.81 0.63 0.7 0.66


Localization RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3
Cortical Patches 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Bud 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Bud Neck 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Bud Site 0 0 0 3.1 0 0 0 0 0 0 0 0 0 0
Cell Periphery 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Cytoplasm 1.9 1.8 1.3 1.8 0.8 1.3 0.6 0 4.3 5.7 6.7 4.6 5.6 5.2
Endoplasmic Reticulum 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Endosome 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Golgi 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Mitochondria -0.4 -6.3 2.9 1.9 0 4.0 0 0 -7.2 -7.0 -7.8 -7.1 -4.2 -6.0
Nucleus -1.8 -2.6 -4.7 -5.0 -7.7 -6.1 -6.4 -5.6 -2.7 -4.9 -7.4 -8.3 -7.4 -7.1
Nuclear Periphery 0 0 0 0 0 3.3 0 0 0 0 0 0 0 0
Nucleolus 1.8 0.9 2.0 3.8 0 2.8 0 0 -0.3 1.3 2.0 1.5 0 0
Peroxisomes 0 0 0 0 0 0 0 0 0 0 0 0 0 0
SpindlePole 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Vacuole -1.7 0.3 -1.7 -1.0 -1.7 0.2 -1.6 -2.0 -0.7 0.9 1.9 6.8 6.4 6.1


Endocytosis
Temp Actin Patch (Sac6-tdTomato) Cortical Patch (Sla1-GFP) Late Endosome (Snf7-GFP) Vacuole (Vph1-GFP)
37℃
RT
Cell Cycle Omics

CYCLoPs (Pop6-GFP)
Gene / Allele Actin Patch (Sac6-tdTomato) Cortical Patch (Sla1-GFP) Late Endosome (Snf7-GFP) Vacuole (Sac6-tdTomato)
Gene Images
Gene Images
Images are not yet available
Images are not yet available