Standard name
Description C-4 methyl sterol oxidase; catalyzes the first of three steps required to remove two C-4 methyl groups from an intermediate in ergosterol biosynthesis; mutants accumulate the sterol intermediate 4,4-dimethylzymosterol; human MSMO1 functionally complements the growth defect caused by repression of ERG25 expression
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Micrographs

ensLOC DeepLoc
Localization WT1 WT2 WT3 RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3 WT1 WT2 WT3 AF100 AF140 AF180
Cortical Patches 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Bud 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Bud Neck 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Bud Site 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 – – – – – –
Cell Periphery 0 0 0 0 0 0.05 0.07 0.05 0.07 0.06 0.08 0.09 0 0 0 0 0 0 0 0 0 0 0 0
Cytoplasm 0.68 0.49 0.26 0.5 0.44 0.58 0.5 0.53 0.66 0.65 0.7 0.65 0.28 0.21 0.42 0.47 0.43 0.5 0.12 0.13 0 0 0 0
Endoplasmic Reticulum 0.16 0.45 0.65 0.5 0.56 0.41 0.48 0.46 0.31 0.31 0.28 0.3 0.64 0.63 0.48 0 0 0.05 0.45 0.56 0.81 0.51 0.5 0.44
Endosome 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Golgi 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Mitochondria 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Nucleus 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Nuclear Periphery 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Nucleolus 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Peroxisomes 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
SpindlePole 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Vac/Vac Membrane 0.19 0.06 0.13 0 0 0 0 0 0 0.05 0 0 0.09 0.21 0.12 0.55 0.57 0.47 0.32 0.22 0.15 0.43 0.4 0.43
ensLOC DeepLoc
Localization WT1 WT2 WT3 RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3 WT1 WT2 WT3 AF100 AF140 AF180
Cortical Patches 0 0 0 1 0 2 4 4 3 5 3 6 0 1 2 1 0 0 0 2 0 0 1 1
Bud 0 11 2 2 1 3 1 9 6 3 2 5 1 2 2 1 0 0 1 6 0 1 3 4
Bud Neck 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 2 0 0 0 0
Bud Site 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 – – – – – –
Cell Periphery 0 1 0 4 15 18 37 24 31 30 30 41 1 3 4 2 4 1 0 0 0 0 0 0
Cytoplasm 47 144 62 112 156 204 273 264 288 344 268 303 54 36 80 81 69 74 11 54 7 2 4 6
Endoplasmic Reticulum 11 132 157 112 202 144 259 229 134 164 106 140 122 111 92 1 4 7 43 231 231 80 130 104
Endosome 0 1 0 0 0 0 0 0 0 0 0 0 0 1 1 0 0 0 0 4 0 0 3 1
Golgi 0 0 3 0 1 0 0 0 0 1 0 0 3 4 3 6 2 2 0 0 0 0 6 1
Mitochondria 1 1 0 0 0 0 0 0 0 0 0 1 0 0 0 1 1 1 0 1 0 0 1 0
Nucleus 0 1 0 1 0 0 0 1 0 0 0 0 0 0 2 2 2 4 2 4 0 0 0 1
Nuclear Periphery 0 0 0 0 0 0 1 0 1 0 0 0 0 0 0 0 0 0 1 7 1 0 1 4
Nucleolus 0 0 0 0 0 0 1 1 1 0 1 4 0 0 0 0 0 0 0 0 0 0 0 0
Peroxisomes 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
SpindlePole 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 1
Vac/Vac Membrane 13 19 32 7 6 4 17 10 13 24 8 10 17 36 23 95 91 69 30 89 41 68 105 102
Unique Cell Count 69 293 243 226 358 349 543 498 435 526 383 468 190 175 192 173 160 147 96 411 286 157 263 237
Labelled Cell Count 72 310 256 239 381 375 593 542 477 571 418 510 198 194 209 190 173 158 96 411 286 157 263 237


Endoplasmic Reticulum, Vacuole

(Huh et al. Nature 2003 425:686)
Screen WT1 WT2 WT3 RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3 AF100 AF140 AF180
Mean Cell GFP Intensity (1e-4) 53.3 63.8 49.8 51.4 55.3 46.6 46.6 46.4 48.9 45.6 46.2 45.2 48.6 36.6 34.5 41.1 41.6 42.1 54.5 52.0 51.0
Std Deviation (1e-4) 24.7 17.6 11.4 11.0 11.2 9.3 9.1 11.0 10.7 10.3 10.6 10.3 11.5 9.6 8.7 12.1 13.2 12.8 14.5 15.6 19.0
Intensity Change (Log2) – – – 0.05 0.15 -0.1 -0.09 -0.1 -0.03 -0.13 -0.11 -0.14 -0.03 -0.44 -0.53 -0.28 -0.26 -0.24 0.13 0.06 0.03


Localization RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3
Cortical Patches 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Bud 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Bud Neck 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Bud Site 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Cell Periphery 0 3.2 3.6 4.2 3.5 4.3 3.8 4.5 4.8 0 0 0 0 0 0
Cytoplasm 5.4 4.5 7.9 6.5 7.1 10.2 10.3 10.9 9.9 0.7 -1.2 3.6 4.5 3.7 5.0
Endoplasmic Reticulum -3.3 -2.0 -5.6 -4.4 -4.8 -8.5 -8.7 -9.1 -8.9 -0.1 -0.2 -3.5 -13.3 -12.5 -11.6
Endosome 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Golgi 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Mitochondria 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Nucleus 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Nuclear Periphery 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Nucleolus 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Peroxisomes 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
SpindlePole 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Vacuole -3.9 -5.7 -6.0 -5.4 -6.2 -5.1 -4.3 -5.5 -5.9 -1.4 2.0 -0.4 9.1 9.3 7.4