Standard name
Description Component of the TOM (translocase of outer membrane) complex; responsible for recognition and initial import steps for all mitochondrially directed proteins; acts as a receptor for incoming precursor proteins
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Micrographs

ensLOC DeepLoc
Localization WT1 WT2 WT3 RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3 WT1 WT2 WT3 AF100 AF140 AF180
Cortical Patches 0 0 0 0 0 0 0 0 – 0 0 0 0 0 0 – – – 0 0 0 0.07 0.06 0.07
Bud 0 0 0 0 0 0 0 0 – 0 0 0 0 0 0 – – – 0 0 0 0 0 0
Bud Neck 0 0 0 0 0 0 0 0 – 0 0 0 0 0 0 – – – 0 0 0 0 0 0
Bud Site 0 0 0 0 0 0 0 0 – 0 0 0 0 0 0 – – – – – – – – –
Cell Periphery 0 0 0 0 0 0 0 0 – 0 0 0 0 0 0 – – – 0 0 0 0 0 0
Cytoplasm 0 0 0 0 0 0 0 0 – 0 0 0 0 0 0 – – – 0 0 0 0 0 0
Endoplasmic Reticulum 0 0 0 0 0 0 0 0 – 0 0 0 0 0 0 – – – 0 0 0 0 0 0
Endosome 0 0 0 0 0 0 0 0 – 0 0 0 0 0 0 – – – 0 0 0 0 0 0
Golgi 0.07 0.13 0.05 0 0 0 0 0 – 0 0 0 0.36 0.27 0.54 – – – 0.06 0.07 0.06 0.13 0.14 0.15
Mitochondria 0.9 0.82 0.96 0.92 0.9 0.87 0.89 0.84 – 0.9 0.93 0.93 0.76 0.76 0.71 – – – 0.85 0.76 0.85 0.69 0.64 0.64
Nucleus 0 0 0 0 0 0 0 0 – 0 0 0 0 0 0 – – – 0 0 0 0 0 0
Nuclear Periphery 0 0 0 0 0 0.06 0 0 – 0.06 0.09 0.09 0 0 0 – – – 0 0 0 0 0 0
Nucleolus 0.15 0.07 0.14 0.08 0.1 0.13 0.18 0.2 – 0.1 0.12 0.13 0 0 0 – – – 0 0 0 0 0 0
Peroxisomes 0 0 0 0 0 0 0 0 – 0 0 0 0 0 0 – – – 0 0 0 0 0 0
SpindlePole 0 0 0 0 0 0 0 0 – 0 0 0 0 0 0 – – – 0 0 0 0 0 0
Vac/Vac Membrane 0.13 0.17 0 0 0.08 0.06 0 0.05 – 0.06 0 0 0.18 0.22 0.18 – – – 0 0.09 0 0.05 0.05 0.07
ensLOC DeepLoc
Localization WT1 WT2 WT3 RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3 WT1 WT2 WT3 AF100 AF140 AF180
Cortical Patches 1 7 4 0 1 2 0 1 – 1 1 1 8 6 16 – – – 5 7 2 12 15 19
Bud 1 1 1 5 8 14 13 6 – 13 12 10 0 1 1 – – – 0 2 0 0 1 3
Bud Neck 0 0 0 0 0 0 0 0 – 0 0 0 0 0 0 – – – 0 0 0 0 1 0
Bud Site 0 0 0 0 1 3 3 0 – 6 5 2 0 0 0 – – – – – – – – –
Cell Periphery 1 9 1 5 5 3 2 2 – 2 4 1 8 4 3 – – – 0 0 0 0 0 0
Cytoplasm 1 2 0 7 12 17 17 19 – 9 12 9 3 5 3 – – – 0 1 0 0 0 0
Endoplasmic Reticulum 0 0 0 2 0 3 0 2 – 2 3 6 1 0 2 – – – 0 0 0 0 0 2
Endosome 0 0 1 1 1 1 0 2 – 0 1 0 0 0 1 – – – 1 1 0 1 1 1
Golgi 21 36 6 7 8 2 1 1 – 5 1 2 86 96 206 – – – 21 20 8 22 36 44
Mitochondria 276 230 125 239 300 349 490 474 – 492 506 560 184 271 270 – – – 280 229 114 126 169 194
Nucleus 1 3 0 0 1 4 3 3 – 0 3 3 1 0 0 – – – 0 1 0 0 0 0
Nuclear Periphery 4 2 1 9 12 22 24 17 – 33 48 52 0 3 4 – – – 1 0 0 0 2 1
Nucleolus 45 19 18 21 32 53 101 111 – 54 67 79 10 16 10 – – – 8 4 1 1 10 6
Peroxisomes 2 1 1 1 2 1 3 2 – 2 4 2 0 2 2 – – – 2 2 3 5 7 5
SpindlePole 0 0 1 0 0 0 2 1 – 2 2 0 0 0 1 – – – 1 0 0 0 1 0
Vac/Vac Membrane 40 49 2 10 26 22 21 30 – 32 16 18 43 79 67 – – – 6 27 1 9 13 22
Unique Cell Count 305 281 130 259 334 400 548 567 547 543 601 241 356 378 331 301 134 182 263 304
Labelled Cell Count 393 359 161 307 409 496 680 671 653 685 745 344 483 586 331 301 134 182 263 304


Mitochondria

(Huh et al. Nature 2003 425:686)
Screen WT1 WT2 WT3 RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3 AF100 AF140 AF180
Mean Cell GFP Intensity (1e-4) 30.8 31.2 18.9 19.3 17.5 14.8 13.4 13.8 – 13.2 11.8 12.8 27.8 28.1 27.8 – – – 31.4 33.4 32.5
Std Deviation (1e-4) 8.9 8.5 4.5 4.8 4.8 4.4 4.3 4.1 – 4.4 4.2 4.3 7.2 6.6 6.0 – – – 9.2 10.5 9.3
Intensity Change (Log2) – – – 0.03 -0.11 -0.35 -0.49 -0.45 – -0.52 -0.67 -0.56 0.56 0.57 0.56 – – – 0.73 0.82 0.78


Localization RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3
Cortical Patches 0 0 0 0 0 – 0 0 0 0 0 0.6 – – –
Bud 0 0 0 0 0 – 0 0 0 0 0 0 – – –
Bud Neck 0 0 0 0 0 – 0 0 0 0 0 0 – – –
Bud Site 0 0 0 0 0 – 0 0 0 0 0 0 – – –
Cell Periphery 0 0 0 0 0 – 0 0 0 0 0 0 – – –
Cytoplasm 0 0 0 0 0 – 0 0 0 0 0 0 – – –
Endoplasmic Reticulum 0 0 0 0 0 – 0 0 0 0 0 0 – – –
Endosome 0 0 0 0 0 – 0 0 0 0 0 0 – – –
Golgi 0 0 0 0 0 – 0 0 0 6.6 5.4 9.9 – – –
Mitochondria -1.5 -2.2 -2.9 -2.4 -3.7 – -2.2 -1.3 -1.3 -4.9 -5.0 -5.8 – – –
Nucleus 0 0 0 0 0 – 0 0 0 0 0 0 – – –
Nuclear Periphery 0 0 2.3 0 0 – 2.5 3.2 3.1 0 0 0 – – –
Nucleolus -1.8 -1.3 -0.2 1.2 1.5 – -1.3 -0.5 -0.2 -3.4 -3.6 -4.8 – – –
Peroxisomes 0 0 0 0 0 – 0 0 0 0 0 0 – – –
SpindlePole 0 0 0 0 0 – 0 0 0 0 0 0 – – –
Vacuole 0 2.5 1.9 0 1.8 – 2.0 0 0 4.6 5.4 4.6 – – –