Standard name
Description TFIIF (Transcription Factor II) largest subunit; involved in both transcription initiation and elongation of RNA polymerase II; homologous to human RAP74

Micrographs

ensLOC DeepLoc
Localization WT1 WT2 WT3 RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3 WT1 WT2 WT3 AF100 AF140 AF180
Cortical Patches 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Bud 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Bud Neck 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Bud Site 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Cell Periphery 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Cytoplasm 0 0 0 0 0 0 0 0 0 0 0 0 0 0.05 0.08 0.08 0.07 0.05 0 0 0 0 0 0
Endoplasmic Reticulum 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Endosome 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Golgi 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Mitochondria 0 0 0 0 0 0.05 0 0.06 0.17 0.23 0.26 0.16 0 0 0 0 0 0 0 0 0 0 0 0
Nucleus 0.92 0.89 0.95 0.96 0.96 0.92 0.93 0.88 0.86 0.83 0.82 0.9 0.92 0.89 0.85 0.85 0.85 0.9 0.85 0.89 0.91 0.82 0.73 0.66
Nuclear Periphery 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Nucleolus 0.36 0.21 0.06 0.05 0 0.11 0.11 0.1 0.22 0.14 0.22 0.12 0.19 0.21 0.21 0.1 0.09 0.1 0.08 0 0.06 0.11 0.18 0.21
Peroxisomes 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
SpindlePole 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Vac/Vac Membrane 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0.05 0.08
ensLOC DeepLoc
Localization WT1 WT2 WT3 RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3 WT1 WT2 WT3 AF100 AF140 AF180
Cortical Patches 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Bud 0 0 0 0 0 1 0 1 2 5 4 1 0 0 1 0 0 0 1 2 1 1 2 3
Bud Neck 0 0 0 0 0 0 0 2 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 3
Bud Site 0 0 0 0 0 0 2 11 5 13 11 5 0 0 0 0 0 0
Cell Periphery 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 2 0 0 0 0 0 0 0 0
Cytoplasm 1 2 3 8 5 6 9 20 6 8 9 2 10 18 41 15 9 6 0 0 0 0 0 0
Endoplasmic Reticulum 0 1 0 0 0 0 0 0 0 1 0 0 0 1 0 3 0 0 0 0 0 1 1 1
Endosome 0 0 0 0 0 0 1 3 0 0 0 1 1 0 3 1 1 0 0 0 1 0 3 3
Golgi 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 1 0 1
Mitochondria 2 7 0 2 2 19 15 33 66 103 90 56 5 2 3 3 2 0 0 0 0 0 1 3
Nucleus 84 149 202 288 348 343 429 490 328 374 282 316 348 317 434 160 105 104 83 161 204 166 290 334
Nuclear Periphery 0 0 0 1 1 4 4 6 0 1 3 2 0 1 1 0 1 0 0 0 0 0 0 0
Nucleolus 33 35 12 15 13 41 52 58 82 62 77 44 73 75 109 18 11 12 7 5 12 21 73 106
Peroxisomes 0 0 0 0 0 1 0 1 0 0 0 0 0 2 3 1 2 0 0 0 0 0 0 0
SpindlePole 0 0 4 1 0 1 3 5 1 4 1 4 1 3 5 1 3 1 1 2 0 0 2 6
Vac/Vac Membrane 0 3 2 1 3 1 2 6 1 1 3 3 0 2 6 3 2 2 2 6 3 8 21 38
Unique Cell Count 91 167 212 299 361 373 459 560 380 449 343 353 380 358 513 189 123 116 98 180 226 204 399 506
Labelled Cell Count 120 197 223 316 372 417 517 636 491 572 480 434 438 421 606 207 136 125 98 180 226 204 399 506


Nucleus

(Huh et al. Nature 2003 425:686)
Screen WT1 WT2 WT3 RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3 AF100 AF140 AF180
Mean Cell GFP Intensity (1e-4) 8.2 8.7 8.5 8.2 9.0 6.9 6.9 6.7 5.5 5.4 5.3 5.8 9.8 9.3 8.9 13.2 15.1 13.5 8.3 7.9 8.5
Std Deviation (1e-4) 1.6 1.9 1.6 1.6 1.5 1.4 1.3 1.6 1.1 1.2 1.1 1.1 2.5 2.3 2.6 5.2 5.2 4.5 2.1 1.8 2.1
Intensity Change (Log2) -0.05 0.08 -0.29 -0.3 -0.34 -0.63 -0.66 -0.69 -0.56 0.2 0.13 0.07 0.64 0.83 0.66 -0.04 -0.1 -0.01


Localization RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3
Cortical Patches 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Bud 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Bud Neck 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Bud Site 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Cell Periphery 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Cytoplasm 0 0 0 0 1.6 0 0 0 0 0 2.2 3.4 3.1 0 0
Endoplasmic Reticulum 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Endosome 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Golgi 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Mitochondria 0 0 3.3 0 3.6 6.4 7.6 8.1 6.1 0 0 0 0 0 0
Nucleus 0.6 0.7 -1.5 -0.9 -3.2 -3.4 -4.3 -4.5 -2.4 -1.7 -2.7 -4.0 -3.6 -3.2 -1.9
Nuclear Periphery 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Nucleolus -0.3 -1.2 2.2 2.3 2.0 5.1 3.1 5.2 2.6 4.5 4.9 5.1 1.5 1.1 1.6
Peroxisomes 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
SpindlePole 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Vacuole 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0


Endocytosis
Temp Actin Patch (Sac6-tdTomato) Cortical Patch (Sla1-GFP) Late Endosome (Snf7-GFP) Vacuole (Vph1-GFP)
37℃
RT
Cell Cycle Omics

CYCLoPs (Tfg1-GFP)
Gene / Allele Actin Patch (Sac6-tdTomato) Cortical Patch (Sla1-GFP) Late Endosome (Snf7-GFP) Vacuole (Sac6-tdTomato)
Gene Images
Gene Images
Images are not yet available
Images are not yet available