Standard name
Description TFIIF (Transcription Factor II) largest subunit; involved in both transcription initiation and elongation of RNA polymerase II; homologous to human RAP74
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Micrographs

ensLOC DeepLoc
Localization WT1 WT2 WT3 RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3 WT1 WT2 WT3 AF100 AF140 AF180
Cortical Patches 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Bud 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Bud Neck 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Bud Site 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 – – – – – –
Cell Periphery 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Cytoplasm 0 0 0 0 0 0 0 0 0 0 0 0 0 0.05 0.08 0.08 0.07 0.05 0 0 0 0 0 0
Endoplasmic Reticulum 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Endosome 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Golgi 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Mitochondria 0 0 0 0 0 0.05 0 0.06 0.17 0.23 0.26 0.16 0 0 0 0 0 0 0 0 0 0 0 0
Nucleus 0.92 0.89 0.95 0.96 0.96 0.92 0.93 0.88 0.86 0.83 0.82 0.9 0.92 0.89 0.85 0.85 0.85 0.9 0.85 0.89 0.91 0.82 0.73 0.66
Nuclear Periphery 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Nucleolus 0.36 0.21 0.06 0.05 0 0.11 0.11 0.1 0.22 0.14 0.22 0.12 0.19 0.21 0.21 0.1 0.09 0.1 0.08 0 0.06 0.11 0.18 0.21
Peroxisomes 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
SpindlePole 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Vac/Vac Membrane 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0.05 0.08
ensLOC DeepLoc
Localization WT1 WT2 WT3 RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3 WT1 WT2 WT3 AF100 AF140 AF180
Cortical Patches 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Bud 0 0 0 0 0 1 0 1 2 5 4 1 0 0 1 0 0 0 1 2 1 1 2 3
Bud Neck 0 0 0 0 0 0 0 2 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 3
Bud Site 0 0 0 0 0 0 2 11 5 13 11 5 0 0 0 0 0 0 – – – – – –
Cell Periphery 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 2 0 0 0 0 0 0 0 0
Cytoplasm 1 2 3 8 5 6 9 20 6 8 9 2 10 18 41 15 9 6 0 0 0 0 0 0
Endoplasmic Reticulum 0 1 0 0 0 0 0 0 0 1 0 0 0 1 0 3 0 0 0 0 0 1 1 1
Endosome 0 0 0 0 0 0 1 3 0 0 0 1 1 0 3 1 1 0 0 0 1 0 3 3
Golgi 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 1 0 1
Mitochondria 2 7 0 2 2 19 15 33 66 103 90 56 5 2 3 3 2 0 0 0 0 0 1 3
Nucleus 84 149 202 288 348 343 429 490 328 374 282 316 348 317 434 160 105 104 83 161 204 166 290 334
Nuclear Periphery 0 0 0 1 1 4 4 6 0 1 3 2 0 1 1 0 1 0 0 0 0 0 0 0
Nucleolus 33 35 12 15 13 41 52 58 82 62 77 44 73 75 109 18 11 12 7 5 12 21 73 106
Peroxisomes 0 0 0 0 0 1 0 1 0 0 0 0 0 2 3 1 2 0 0 0 0 0 0 0
SpindlePole 0 0 4 1 0 1 3 5 1 4 1 4 1 3 5 1 3 1 1 2 0 0 2 6
Vac/Vac Membrane 0 3 2 1 3 1 2 6 1 1 3 3 0 2 6 3 2 2 2 6 3 8 21 38
Unique Cell Count 91 167 212 299 361 373 459 560 380 449 343 353 380 358 513 189 123 116 98 180 226 204 399 506
Labelled Cell Count 120 197 223 316 372 417 517 636 491 572 480 434 438 421 606 207 136 125 98 180 226 204 399 506


Nucleus

(Huh et al. Nature 2003 425:686)
Screen WT1 WT2 WT3 RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3 AF100 AF140 AF180
Mean Cell GFP Intensity (1e-4) 8.2 8.7 8.5 8.2 9.0 6.9 6.9 6.7 5.5 5.4 5.3 5.8 9.8 9.3 8.9 13.2 15.1 13.5 8.3 7.9 8.5
Std Deviation (1e-4) 1.6 1.9 1.6 1.6 1.5 1.4 1.3 1.6 1.1 1.2 1.1 1.1 2.5 2.3 2.6 5.2 5.2 4.5 2.1 1.8 2.1
Intensity Change (Log2) – – – -0.05 0.08 -0.29 -0.3 -0.34 -0.63 -0.66 -0.69 -0.56 0.2 0.13 0.07 0.64 0.83 0.66 -0.04 -0.1 -0.01


Localization RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3
Cortical Patches 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Bud 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Bud Neck 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Bud Site 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Cell Periphery 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Cytoplasm 0 0 0 0 1.6 0 0 0 0 0 2.2 3.4 3.1 0 0
Endoplasmic Reticulum 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Endosome 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Golgi 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Mitochondria 0 0 3.3 0 3.6 6.4 7.6 8.1 6.1 0 0 0 0 0 0
Nucleus 0.6 0.7 -1.5 -0.9 -3.2 -3.4 -4.3 -4.5 -2.4 -1.7 -2.7 -4.0 -3.6 -3.2 -1.9
Nuclear Periphery 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Nucleolus -0.3 -1.2 2.2 2.3 2.0 5.1 3.1 5.2 2.6 4.5 4.9 5.1 1.5 1.1 1.6
Peroxisomes 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
SpindlePole 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Vacuole 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0

Images

Circle plot not available

Protein Concentration and Protein Localization Data

R1 R2 R3
G1 Pre-START G1 Post-START S/G2 Metaphase Anaphase Telophase G1 Pre-START G1 Post-START S/G2 Metaphase Anaphase Telophase G1 Pre-START G1 Post-START S/G2 Metaphase Anaphase Telophase
Concentration 3.5379 4.3634 3.3766 3.6376 4.7635 4.0281 4.5514 5.6114 5.0253 4.5239 3.9301 4.7626 5.4404 6.636 5.8849 6.1846 4.2362 5.1944
Actin 0.0821 0 0.0121 0.083 0.0006 0.0224 0.0154 0 0 0.001 0.0012 0 0.0029 0 0.0002 0 0.011 0.0026
Bud 0.001 0.0001 0.0003 0.0005 0.0001 0.0003 0.001 0 0 0.0002 0.0001 0 0.0001 0 0.0003 0 0.0001 0.0007
Bud Neck 0.0035 0.0001 0.0006 0.0003 0.0004 0.0005 0.0007 0 0 0.0001 0.0004 0.0007 0.0002 0 0.0001 0 0.0002 0.0008
Bud Periphery 0.0013 0.0003 0.0003 0.0008 0.0001 0.0005 0.0023 0 0 0.0004 0.0004 0.0001 0.0002 0 0.0004 0.0001 0.0002 0.001
Bud Site 0.0074 0 0.0022 0.0007 0.0001 0.0004 0.0031 0 0.0002 0.0007 0.0004 0 0.0006 0 0.001 0.0001 0.0003 0.0012
Cell Periphery 0.0006 0 0.0001 0.0001 0 0.0001 0.0009 0 0 0.0001 0.0003 0 0 0 0.0002 0.0002 0.0001 0.0001
Cytoplasm 0.0385 0.0001 0.0045 0.0022 0.0373 0.0026 0.0163 0 0.0001 0.0001 0.0001 0 0.0086 0 0.0007 0.0001 0.0007 0.0011
Cytoplasmic Foci 0.05 0 0.0244 0.0024 0.0015 0.0005 0.0382 0 0.0018 0.0027 0.0029 0 0.0135 0 0.0073 0.004 0.0112 0.0025
Eisosomes 0.0007 0 0.0001 0.0002 0 0 0.0002 0 0 0.0001 0.0001 0 0.0001 0 0 0 0.0001 0
Endoplasmic Reticulum 0.0113 0 0.0012 0.0155 0.0005 0.0005 0.0044 0 0 0.0001 0.0003 0 0.0032 0 0.0002 0.0001 0.0018 0.0005
Endosome 0.0503 0.0001 0.0164 0.0194 0.0024 0.0015 0.0222 0 0.0004 0.0156 0.0065 0 0.016 0 0.0006 0.0017 0.0189 0.0062
Golgi 0.0142 0 0.0107 0.0082 0.0006 0.0011 0.0048 0 0.0001 0.0123 0.007 0 0.0011 0 0.0002 0.0007 0.0116 0.0024
Lipid Particles 0.0184 0 0.0116 0.0026 0.0053 0.0006 0.0073 0 0.0015 0.0103 0.082 0 0.0066 0 0.0022 0.034 0.0675 0.0022
Mitochondria 0.0059 0.0003 0.0048 0.0043 0.0006 0.0011 0.0104 0.0001 0.0001 0.0295 0.0753 0.0001 0.0019 0.0002 0.0017 0.0044 0.0054 0.0043
None 0.0112 0.0001 0.0005 0.0006 0.0006 0.0002 0.0307 0 0 0.0001 0.0002 0 0.0046 0 0.0006 0.0001 0.0021 0.0004
Nuclear Periphery 0.0403 0.0014 0.003 0.01 0.0757 0.0181 0.0261 0.0002 0.0009 0.0007 0.0059 0.0001 0.016 0.0001 0.0006 0.0012 0.0031 0.013
Nucleolus 0.029 0.0316 0.0211 0.0099 0.259 0.1225 0.0241 0.0181 0.0138 0.0136 0.2572 0.1146 0.0331 0.0228 0.0251 0.0181 0.1314 0.0914
Nucleus 0.5644 0.964 0.8628 0.8365 0.5071 0.8221 0.7324 0.9781 0.9758 0.9051 0.5324 0.8841 0.8601 0.9766 0.9519 0.9248 0.7249 0.861
Peroxisomes 0.0281 0 0.0128 0.0003 0.0004 0.0005 0.0294 0 0 0.0014 0.0023 0 0.0006 0 0.0004 0.0006 0.0061 0.001
Punctate Nuclear 0.0316 0.001 0.0096 0.0008 0.1064 0.0013 0.0212 0.0034 0.0049 0.0003 0.0116 0.0002 0.0289 0 0.0023 0.0007 0.0021 0.0057
Vacuole 0.0056 0.0003 0.0007 0.0007 0.0005 0.0014 0.0041 0 0.0002 0.0025 0.0031 0.0001 0.0009 0 0.0019 0.0035 0.0004 0.001
Vacuole Periphery 0.0045 0.0005 0.0003 0.001 0.0009 0.0017 0.0045 0 0.0001 0.0029 0.01 0 0.0009 0 0.0019 0.0056 0.0009 0.001

Sequencing Data

R1 R2
G1 Post-START S/G2 Metaphase Anaphase Telophase G1 Post-START S/G2 Metaphase Anaphase Telophase
Gene Expression 35.9806 35.7383 39.779 47.6126 43.0868 54.9399 42.1198 42.0704 51.3205 45.1659
Translational Efficiency 2.0141 1.9134 1.6766 1.6466 1.5575 1.3457 1.4481 1.5956 1.2221 1.3579

Hit Data

Dataset Hit
Protein Abundance ✘
Protein Localization ✘
Gene Expression ✘
Translational Efficiency ✘