Standard name
Description Vacuolar transporter; exports large neutral amino acids from the vacuole; member of a family of seven S. cerevisiae genes (AVT1-7) related to vesicular GABA-glycine transporters
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Micrographs

ensLOC DeepLoc
Localization WT1 WT2 WT3 RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3 WT1 WT2 WT3 AF100 AF140 AF180
Cortical Patches 0 0.06 0 0 0 0 0 0 – 0 0 0 0 0 0 – – – 0 0.06 0.09 0.05 0.05 0
Bud 0 0 0 0 0 0 0 0 – 0 0.05 0.05 0 0 0 – – – 0 0 0 0 0 0
Bud Neck 0 0 0 0 0 0 0 0 – 0 0 0 0 0 0 – – – 0 0 0 0 0 0
Bud Site 0 0 0 0 0 0 0 0 – 0 0 0 0 0 0 – – – – – – – – –
Cell Periphery 0 0.11 0.11 0.09 0.07 0 0 0 – 0 0 0 0.07 0.11 0.12 – – – 0 0.05 0.08 0.11 0.12 0.12
Cytoplasm 0.69 0.52 0.68 0.62 0.62 0.57 0.59 0.61 – 0.57 0.62 0.6 0.81 0.9 0.96 – – – 0.17 0.18 0.1 0.1 0.14 0.18
Endoplasmic Reticulum 0.17 0.33 0.16 0.11 0.12 0 0 0 – 0 0 0 0.15 0.06 0 – – – 0.54 0.51 0.55 0.46 0.41 0.38
Endosome 0 0 0 0 0 0 0 0 – 0 0 0 0 0 0 – – – 0 0 0 0 0 0
Golgi 0 0 0 0 0 0 0 0 – 0 0 0 0 0 0 – – – 0 0 0 0 0 0
Mitochondria 0.08 0.12 0.11 0.32 0.26 0.5 0.5 0.52 – 0.6 0.57 0.57 0 0 0 – – – 0.1 0.06 0.11 0.19 0.15 0.16
Nucleus 0 0 0 0 0 0 0 0 – 0 0 0 0 0 0 – – – 0 0 0 0 0 0
Nuclear Periphery 0 0 0 0 0 0 0 0 – 0 0 0 0 0 0 – – – 0 0 0 0 0 0
Nucleolus 0 0 0 0 0 0 0 0 – 0 0 0 0 0 0 – – – 0 0 0 0 0 0
Peroxisomes 0 0 0 0 0 0 0 0 – 0 0 0 0 0 0 – – – 0 0 0 0 0 0
SpindlePole 0 0 0 0 0 0 0 0 – 0 0 0 0 0 0 – – – 0 0 0 0 0 0
Vac/Vac Membrane 0 0 0 0 0.06 0 0 0 – 0 0 0 0 0 0 – – – 0 0 0 0 0.05 0
ensLOC DeepLoc
Localization WT1 WT2 WT3 RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3 WT1 WT2 WT3 AF100 AF140 AF180
Cortical Patches 5 9 13 6 0 0 0 1 – 0 0 0 3 4 2 – – – 7 12 35 12 19 18
Bud 1 5 6 9 9 7 12 14 – 19 29 44 0 1 0 – – – 1 3 1 1 3 4
Bud Neck 1 0 3 0 0 0 1 0 – 0 0 1 0 1 2 – – – 4 8 6 7 8 10
Bud Site 0 0 0 0 0 0 0 3 – 0 2 1 0 0 0 – – – – – – – – –
Cell Periphery 4 16 35 23 23 12 14 11 – 16 14 23 9 17 27 – – – 5 9 29 24 42 52
Cytoplasm 92 75 213 153 198 218 343 401 – 340 371 531 100 137 220 – – – 31 34 38 22 51 74
Endoplasmic Reticulum 23 48 49 27 38 13 11 7 – 9 1 8 19 9 7 – – – 99 96 214 105 146 159
Endosome 1 0 2 1 8 2 4 1 – 0 1 2 0 0 0 – – – 1 2 2 0 1 3
Golgi 1 0 0 0 0 0 0 0 – 0 0 0 0 0 0 – – – 3 0 0 0 4 1
Mitochondria 11 17 35 79 82 191 286 338 – 358 342 509 0 0 0 – – – 19 11 43 43 55 65
Nucleus 0 0 0 2 0 0 0 2 – 3 1 2 0 0 1 – – – 0 0 0 0 0 0
Nuclear Periphery 1 0 1 0 1 0 1 2 – 2 1 2 0 0 0 – – – 0 1 0 0 1 1
Nucleolus 0 0 0 0 0 0 0 2 – 2 1 0 0 0 0 – – – 0 0 0 0 0 1
Peroxisomes 0 0 0 1 0 0 0 0 – 0 0 1 0 0 0 – – – 0 0 0 0 0 0
SpindlePole 1 0 0 0 1 1 2 4 – 3 9 9 0 0 0 – – – 0 0 0 0 0 1
Vac/Vac Membrane 2 1 10 3 19 15 24 14 – 11 6 6 0 2 0 – – – 5 7 8 7 18 16
Unique Cell Count 134 144 313 248 321 385 577 653 593 600 890 123 152 229 184 191 390 228 360 420
Labelled Cell Count 143 171 367 304 379 459 698 800 763 778 1139 131 171 259 184 191 390 228 360 420


Endoplasmic Reticulum

(Huh et al. Nature 2003 425:686)
Screen WT1 WT2 WT3 RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3 AF100 AF140 AF180
Mean Cell GFP Intensity (1e-4) 5.2 5.2 4.5 4.7 4.7 3.8 3.6 3.7 – 3.2 3.2 3.2 6.2 6.3 6.0 – 4.3 – 6.4 6.9 6.8
Std Deviation (1e-4) 0.8 0.7 0.9 1.3 1.0 1.4 0.9 0.9 – 0.9 0.9 0.7 0.7 0.9 0.7 – 0.9 – 1.0 1.6 1.5
Intensity Change (Log2) – – – 0.07 0.05 -0.23 -0.3 -0.28 – -0.51 -0.48 -0.49 0.46 0.48 0.42 – -0.07 – 0.51 0.62 0.59


Localization RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3
Cortical Patches 0 0 0 0 0 – 0 0 0 0 -0.8 -2.3 – – –
Bud 0 0 0 0 0 – 0 0 0 0 0 0 – – –
Bud Neck 0 0 0 0 0 – 0 0 0 0 0 0 – – –
Bud Site 0 0 0 0 0 – 0 0 0 0 0 0 – – –
Cell Periphery 0 0 0 0 0 – 0 0 0 -1.2 0 0.2 – – –
Cytoplasm -1.6 -1.7 -3.1 -2.5 -2.0 – -3.1 -1.9 -2.6 2.8 5.2 8.0 – – –
Endoplasmic Reticulum 0 0 0 0 0 – 0 0 0 -0.1 -3.0 -4.8 – – –
Endosome 0 0 0 0 0 – 0 0 0 0 0 0 – – –
Golgi 0 0 0 0 0 – 0 0 0 0 0 0 – – –
Mitochondria 0 0 0 0 0 – 0 0 0 -3.9 -4.3 -5.2 – – –
Nucleus 0 0 0 0 0 – 0 0 0 0 0 0 – – –
Nuclear Periphery 0 0 0 0 0 – 0 0 0 0 0 0 – – –
Nucleolus 0 0 0 0 0 – 0 0 0 0 0 0 – – –
Peroxisomes 0 0 0 0 0 – 0 0 0 0 0 0 – – –
SpindlePole 0 0 0 0 0 – 0 0 0 0 0 0 – – –
Vacuole -1.6 1.6 0.5 0.7 -1.0 – -1.3 -2.4 0 0 0 0 – – –