Standard name
Description Voltage-gated high-affinity calcium channel; involved in calcium influx in response to some environmental stresses as well as exposure to mating pheromones; interacts and partially co-localizes with Mid1p; however, evidence suggests CCH1 is not required for Mid1p function
DOWNLOAD

Micrographs

ensLOC DeepLoc
Localization WT1 WT2 WT3 RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3 WT1 WT2 WT3 AF100 AF140 AF180
Cortical Patches 0.07 – 0 0 0 0 – 0 0 0 0 0 0 0 0 0.07 0.08 0.05 0.1 0 0.06 0.14 0.18 0.17
Bud 0 – 0.06 0 0 0.05 – 0.05 0.06 0 0.07 0.06 0 0 0 0 0 0 0 0.05 0 0.1 0.11 0.09
Bud Neck 0 – 0 0 0 0 – 0 0 0 0 0 0 0 0 0 0 0 0.06 0.1 0.07 0.12 0.08 0.06
Bud Site 0 – 0 0 0 0 – 0 0 0 0 0 0 0 0 0 0 0 – – – – – –
Cell Periphery 0 – 0 0 0 0 – 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Cytoplasm 0.67 – 0.84 0.78 0.76 0.72 – 0.62 0.55 0.64 0.56 0.45 0.88 0.83 0.86 0.81 0.74 0.84 0.29 0.39 0.38 0.21 0.24 0.3
Endoplasmic Reticulum 0.11 – 0 0 0 0 – 0 0 0 0 0.05 0.05 0 0 0.06 0.06 0.1 0.07 0.05 0 0 0.07 0.05
Endosome 0 – 0 0 0 0 – 0 0 0 0 0 0 0.1 0.08 0.05 0.08 0 0.06 0 0 0 0.05 0
Golgi 0 – 0 0 0 0 – 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Mitochondria 0.14 – 0.12 0.25 0.09 0.27 – 0.37 0.59 0.43 0.54 0.63 0 0 0 0 0 0 0.25 0.15 0.27 0.22 0.15 0.14
Nucleus 0 – 0 0 0 0 – 0 0 0 0 0 0 0 0 0 0 0 0 0.05 0 0 0 0
Nuclear Periphery 0 – 0 0 0 0 – 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Nucleolus 0 – 0 0 0 0 – 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Peroxisomes 0 – 0 0 0 0 – 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
SpindlePole 0 – 0 0 0 0 – 0 0 0 0 0 0 0 0 0 0 0.05 0 0 0 0 0 0
Vac/Vac Membrane 0 – 0 0 0 0 – 0 0 0 0 0 0 0 0 0 0.06 0 0.09 0.14 0.08 0.09 0.08 0.1
ensLOC DeepLoc
Localization WT1 WT2 WT3 RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3 WT1 WT2 WT3 AF100 AF140 AF180
Cortical Patches 18 – 1 7 14 2 – 2 0 6 7 12 9 12 5 10 12 8 30 0 8 13 59 65
Bud 8 – 6 9 16 22 – 41 21 21 28 29 4 22 8 2 5 3 13 1 6 10 34 35
Bud Neck 2 – 3 0 3 1 – 7 3 8 0 1 0 0 1 0 0 0 18 4 9 12 24 22
Bud Site 1 – 1 6 5 4 – 7 3 5 9 4 1 0 0 1 2 0 – – – – – –
Cell Periphery 2 – 1 5 0 3 – 1 0 5 3 6 1 4 1 4 6 1 2 0 1 1 2 5
Cytoplasm 165 – 92 186 300 333 – 470 183 304 232 224 208 417 422 121 106 146 90 16 52 21 79 118
Endoplasmic Reticulum 27 – 1 1 14 7 – 5 0 1 6 26 12 5 10 9 8 17 20 2 2 1 22 19
Endosome 7 – 0 1 17 12 – 18 0 2 2 0 7 52 39 7 11 4 17 1 4 4 15 14
Golgi 0 – 0 0 0 0 – 0 0 0 0 0 1 0 1 3 2 2 4 0 0 0 1 4
Mitochondria 34 – 13 60 36 127 – 283 195 206 223 311 5 9 7 3 4 1 76 6 37 21 47 55
Nucleus 2 – 0 0 0 0 – 4 1 3 0 2 0 3 2 1 0 1 0 1 0 0 0 0
Nuclear Periphery 0 – 0 1 0 0 – 3 0 1 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Nucleolus 1 – 0 0 0 0 – 3 0 1 0 1 0 0 0 0 0 0 0 0 0 0 0 0
Peroxisomes 0 – 0 5 9 5 – 3 0 2 0 1 1 12 4 3 2 0 1 0 0 0 2 0
SpindlePole 1 – 0 4 11 11 – 29 10 13 8 4 2 3 6 4 3 9 1 0 1 0 3 4
Vac/Vac Membrane 4 – 0 0 8 9 – 8 3 7 4 6 0 5 8 6 8 2 29 5 11 8 27 37
Unique Cell Count 247 109 239 397 465 755 331 475 416 495 237 501 489 150 143 174 311 43 137 99 325 390
Labelled Cell Count 272 118 285 433 536 884 419 585 522 627 251 544 514 174 169 194 311 43 137 99 325 390


Cytoplasm

(Huh et al. Nature 2003 425:686)
Screen WT1 WT2 WT3 RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3 AF100 AF140 AF180
Mean Cell GFP Intensity (1e-4) 4.8 – 3.6 3.8 4.2 3.4 – 3.6 3.1 3.4 3.2 3.1 5.2 5.3 5.2 7.1 7.3 6.7 5.1 5.6 5.5
Std Deviation (1e-4) 0.4 – 0.7 0.9 0.8 0.7 – 1.0 0.9 0.7 0.6 0.6 0.9 1.2 1.2 1.3 2.0 1.5 1.2 1.7 1.3
Intensity Change (Log2) – – – 0.11 0.25 -0.06 – 0.01 -0.21 -0.06 -0.16 -0.21 0.55 0.59 0.54 0.99 1.04 0.92 0.53 0.66 0.63


Localization RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3
Cortical Patches 0 0 0 – 0 0 0 0 0 0 0 0 0 2.7 0
Bud 0 0 0 – 0 0 0 0 0 0 -0.5 0 0 0 0
Bud Neck 0 0 0 – 0 0 0 0 0 0 0 0 0 0 0
Bud Site 0 0 0 – 0 0 0 0 0 0 0 0 0 0 0
Cell Periphery 0 0 0 – 0 0 0 0 0 0 0 0 0 0 0
Cytoplasm -1.4 -2.0 -2.7 – -4.5 -5.4 -4.1 -5.5 -7.4 0.9 -0.3 0.5 -0.8 -2.0 -0.1
Endoplasmic Reticulum 0 0 0 – 0 0 0 0 0 0 0 0 0 0 3.0
Endosome 0 0 0 – 0 0 0 0 0 0 3.5 3.0 0 0 0
Golgi 0 0 0 – 0 0 0 0 0 0 0 0 0 0 0
Mitochondria 0 0 0 – 0 0 0 0 0 -3.8 0 0 -3.3 -2.9 -4.3
Nucleus 0 0 0 – 0 0 0 0 0 0 0 0 0 0 0
Nuclear Periphery 0 0 0 – 0 0 0 0 0 0 0 0 0 0 0
Nucleolus 0 0 0 – 0 0 0 0 0 0 0 0 0 0 0
Peroxisomes 0 0 0 – 0 0 0 0 0 0 0 0 0 0 0
SpindlePole 0 0 0 – 0 0 0 0 0 0 0 0 0 0 0
Vacuole 0 0 0 – 0 0 0 0 0 0 0 0 0 0 0