Standard name
Description Putative 4a-hydroxytetrahydrobiopterin dehydratase; green fluorescent protein (GFP)-fusion protein localizes to mitochondria and is induced in response to the DNA-damaging agent MMS

Micrographs

ensLOC DeepLoc
Localization WT1 WT2 WT3 RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3 WT1 WT2 WT3 AF100 AF140 AF180
Cortical Patches 0.05 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0.05 0
Bud 0 0 0.08 0.08 0 0.05 0.07 0 0.05 0.08 0 0 0 0 0.06 0 0 0 0 0 0
Bud Neck 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Bud Site 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Cell Periphery 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0.06 0 0 0 0 0 0
Cytoplasm 0.35 0.36 0.39 0.2 0.14 0 0.05 0 0.07 0.06 0.05 0.45 0.55 0.75 0.38 0.12 0.06 0.08 0 0 0.05
Endoplasmic Reticulum 0.07 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Endosome 0 0.16 0 0 0.06 0 0 0 0 0 0 0.19 0.18 0.13 0.19 0.08 0.12 0.11 0.09 0.1 0.08
Golgi 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0.05 0
Mitochondria 0.68 0.36 0.6 0.83 0.78 0.91 0.9 0.95 0.93 0.95 0.91 0.22 0.14 0 0.06 0.49 0.52 0.54 0.67 0.53 0.58
Nucleus 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Nuclear Periphery 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Nucleolus 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Peroxisomes 0 0 0 0.05 0.05 0.06 0 0 0 0 0 0 0.06 0 0.19 0 0 0 0 0.05 0
SpindlePole 0 0.13 0.1 0.05 0 0.07 0.08 0.06 0.07 0 0.06 0 0 0.05 0.12 0 0.09 0.05 0.05 0 0
Vac/Vac Membrane 0 0.11 0 0 0.08 0 0.05 0 0 0 0 0.16 0.09 0.07 0.12 0.14 0 0 0.05 0.07 0.08
ensLOC DeepLoc
Localization WT1 WT2 WT3 RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3 WT1 WT2 WT3 AF100 AF140 AF180
Cortical Patches 5 0 1 0 0 0 0 0 0 0 0 2 2 0 0 6 10 5 3 7 3
Bud 2 6 12 14 5 11 24 13 14 18 11 5 1 2 1 0 3 2 1 0 2
Bud Neck 0 1 3 0 0 0 2 0 1 0 1 1 1 1 0 3 4 3 1 3 4
Bud Site 0 0 4 2 0 3 5 7 10 5 7 0 0 0 0
Cell Periphery 0 2 1 5 0 0 2 1 2 1 0 0 0 2 1 1 0 1 0 0 0
Cytoplasm 33 61 61 34 19 8 16 14 21 14 16 85 71 128 6 18 15 14 6 2 5
Endoplasmic Reticulum 7 1 3 0 4 1 0 2 0 0 0 3 3 4 0 3 2 2 0 2 1
Endosome 0 27 0 0 8 7 2 0 3 0 2 35 23 22 3 11 28 19 13 13 9
Golgi 0 1 0 0 0 0 0 0 0 0 0 1 0 1 0 0 4 0 1 7 2
Mitochondria 65 61 93 139 104 211 310 316 276 221 280 42 18 3 1 77 126 93 102 75 76
Nucleus 0 1 0 1 1 5 10 6 4 5 7 0 0 0 0 0 0 0 0 0 0
Nuclear Periphery 1 0 0 0 1 0 2 7 0 6 1 0 0 0 0 0 0 0 0 1 0
Nucleolus 0 2 0 4 1 5 2 8 2 3 5 1 0 0 0 0 1 1 1 1 0
Peroxisomes 0 5 4 9 7 14 4 3 10 5 10 3 8 3 3 1 6 6 2 7 5
SpindlePole 0 23 15 8 4 16 29 21 20 9 19 8 3 9 2 4 21 8 7 4 5
Vac/Vac Membrane 2 19 3 1 11 5 16 13 5 2 12 30 12 12 2 21 4 4 7 9 9
Unique Cell Count 95 171 156 168 133 231 343 333 298 233 309 187 128 171 16 157 243 174 153 141 132
Labelled Cell Count 115 210 200 217 165 286 424 411 368 289 371 216 142 187 19 157 243 174 153 141 132


Mitochondria

(Huh et al. Nature 2003 425:686)
Screen WT1 WT2 WT3 RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3 AF100 AF140 AF180
Mean Cell GFP Intensity (1e-4) 3.8 4.8 3.7 3.7 4.2 3.4 3.5 3.0 3.4 3.1 3.2 4.8 4.9 5.1 6.9 5.0 6.0 5.3
Std Deviation (1e-4) 0.5 1.4 1.9 2.2 1.7 1.1 1.5 0.9 1.0 0.7 0.9 1.1 1.1 1.6 2.4 1.8 2.4 1.9
Intensity Change (Log2) -0.01 0.18 -0.13 -0.06 -0.3 -0.12 -0.24 -0.21 0.37 0.42 0.46 0.9 0.43 0.7 0.54


Localization RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3
Actin
Bud
Bud Neck
Bud Site
Cell Periphery
Cyto
Endoplasmic Reticulum
Endosome
Golgi
Mitochondria
Nuclear Periphery
Nuc
Nucleolus
Peroxisomes
SpindlePole
Vac


Endocytosis
Temp Actin Patch (Sac6-tdTomato) Cortical Patch (Sla1-GFP) Late Endosome (Snf7-GFP) Vacuole (Vph1-GFP)
37℃
RT
Cell Cycle Omics

CYCLoPs (Mco14-GFP)
Gene / Allele Actin Patch (Sac6-tdTomato) Cortical Patch (Sla1-GFP) Late Endosome (Snf7-GFP) Vacuole (Sac6-tdTomato)
Gene Images
Gene Images
Images are not yet available
Images are not yet available