Standard name
Description Subunit of the G protein involved in pheromone response; GTP-binding alpha subunit of the heterotrimeric G protein; negatively regulates the mating pathway by sequestering G(beta)gamma and by triggering an adaptive response; activates Vps34p at the endosome; protein abundance increases in response to DNA replication stress

Micrographs

Sub-cellular Localization

ensLOC DeepLoc
Localization WT1 WT2 WT3 RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3 WT1 WT2 WT3 AF100 AF140 AF180
Cortical Patches 0.23 0.24 0.42 0.45 0.45 0.34 0.32 0.32 0.34 0.30 0.22 0 0 0 0 0
Bud 0.09 0.06 0.08 0.09 0.05 0.09 0.13 0.18 0.05 0 0 0 0 0 0 0
Bud Neck 0 0 0 0 0 0 0 0 0 0 0 0 0 0.06 0 0
Bud Site 0 0 0 0 0 0 0 0 0 0 0
Cell Periphery 0.71 0.78 0.58 0.56 0.63 0.38 0.42 0.39 0.47 0.96 0.88 0 0.86 0.75 0.88 0.92
Cytoplasm 0.12 0 0.11 0.12 0.11 0.23 0.23 0.26 0.17 0.07 0.25 0.27 0 0 0 0
Endoplasmic Reticulum 0 0.07 0.05 0.07 0.08 0.10 0.11 0.05 0.11 0 0 0 0 0 0 0
Endosome 0 0 0 0 0 0 0 0 0 0 0 0.16 0 0 0 0
Golgi 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Mitochondria 0.05 0.10 0 0.17 0.21 0.18 0.32 0.33 0.30 0 0 0.31 0 0.08 0 0
Nucleus 0 0 0 0 0 0 0 0 0 0 0 0.19 0 0 0 0
Nuclear Periphery 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Nucleolus 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Peroxisomes 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
SpindlePole 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Vac/Vac Membrane 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
ensLOC DeepLoc
Localization WT1 WT2 WT3 RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3 WT1 WT2 WT3 AF100 AF140 AF180
Cortical Patches 57 52 64 156 145 89 87 84 82 8 7 0 9 0 1 1
Bud 23 12 12 32 16 25 35 46 13 1 1 0 3 0 1 1
Bud Neck 0 0 0 0 0 0 0 0 0 0 0 0 8 3 0 0
Bud Site 0 0 2 5 2 0 2 0 0 0 0
Cell Periphery 180 169 88 195 201 100 116 102 115 26 28 0 289 39 78 76
Cytoplasm 31 9 17 41 34 61 63 69 41 2 8 1 2 1 1 0
Endoplasmic Reticulum 9 15 8 25 26 27 29 14 27 0 1 0 10 0 2 1
Endosome 0 0 1 1 2 2 2 0 0 0 0 0 0 0 0 0
Golgi 0 0 0 9 1 2 1 0 1 0 0 0 0 0 0 0
Mitochondria 13 21 5 59 68 49 88 86 74 0 0 1 6 4 2 2
Nucleus 1 0 0 5 1 0 1 1 1 0 0 0 0 0 0 0
Nuclear Periphery 2 2 2 3 10 9 9 2 8 0 0 0 0 0 0 0
Nucleolus 0 0 0 1 0 5 0 1 0 0 0 0 0 0 0 0
Peroxisomes 3 1 2 2 2 1 3 0 2 0 0 0 0 0 0 0
SpindlePole 0 0 1 6 0 1 0 0 1 0 0 0 0 0 0 0
Vac/Vac Membrane 2 0 2 9 0 5 0 0 3 0 0 0 3 1 0 0
Unique Cell Count 253 218 151 348 319 265 275 261 244 27 32 4 338 53 89 83
Labelled Cell Count 321 281 204 549 508 376 436 405 368 37 45 4 338 53 89 83

Yeast GFP Assignment

(Huh et al. Nature 2003 425:686 )

Cell Periphery

Protein Abundance

Screen WT1 WT2 WT3 RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3 AF100 AF140 AF180
Mean Cell GFP Intensity (1e-4) 13.2 13.7 12.2 11.1 10.9 10.7 9.9 9.6 10.3 13.4 13.4 18.8 19.6 19.8
Std Deviation (1e-4) 2.9 2.9 2.5 2.4 2.3 2.4 2.4 2.3 2.6 2.3 2.2 3.0 3.1 3.2
Intensity Change (Log2) 0.06 -0.11 -0.24 -0.27 -0.29 -0.42 -0.45 -0.35 0.02 0.02 0.51 0.57 0.59

Localization Change

Localization RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3
Cortical Patches 0.3 4.2 5.6 5.7 2.8 2.3 2.5 2.8 0.8 -0.1
Bud -1.5 -0.4 0 -1.9 0.1 1.3 2.8 -1.6 0 0
Bud Neck 0 0 0 0 0 0 0 0 0 0
Bud Site 0 0 0 0 0 0 0 0 0 0
Cell Periphery 1.6 -2.6 -3.8 -2.0 -7.6 -6.7 -7.3 -5.4 2.8 2.0
Cytoplasm -2.6 -0.1 -0.8 -0.2 2.4 2.9 4.2 2.4 0 0
Endoplasmic Reticulum 1.6 0.8 1.9 2.3 3.0 3.1 1.0 3.2 0 0
Endosome 0 0 0 0 0 0 0 0 0 0
Golgi 0 0 0 0 0 0 0 0 0 0
Mitochondria 1.9 -0.9 4.4 5.5 4.7 7.8 8.0 7.4 0 0
Nucleus 0 0 0 0 0 0 0 0 0 0
Nuclear Periphery 0 0 0 1.9 2.1 2.0 0 0 0 0
Nucleolus 0 0 0 0 0 0 0 0 0 0
Peroxisomes 0 0 0 0 0 0 0 0 0 0
SpindlePole 0 0 0 0 0 0 0 0 0 0
Vacuole 0 0 0 0 0 0 0 0 0 0