Standard name
Human Ortholog
Description Essential protein required for the accumulation of box C/D snoRNA

Micrographs

ensLOC DeepLoc
Localization WT1 WT2 WT3 RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3 WT1 WT2 WT3 AF100 AF140 AF180
Cortical Patches 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Bud 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Bud Neck 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Bud Site 0 0 0 0 0 0 0 0 0 0 0.05 0 0 0 0 0 0 0
Cell Periphery 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Cytoplasm 0 0 0.08 0 0.08 0.1 0.12 0.11 0.09 0.09 0.06 0.06 0.06 0.15 0.19 0.09 0.1 0.06 0 0 0 0 0 0
Endoplasmic Reticulum 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Endosome 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Golgi 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Mitochondria 0.57 0 0 0.09 0.08 0.33 0.58 0.38 0.57 0.52 0.73 0.54 0 0 0 0 0 0 0 0 0 0 0 0
Nucleus 0.88 0.94 0.9 0.94 0.84 0.73 0.59 0.67 0.68 0.69 0.65 0.71 0.93 0.82 0.8 0.81 0.81 0.85 0.84 0.9 0.86 0.8 0.66 0.62
Nuclear Periphery 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Nucleolus 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Peroxisomes 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
SpindlePole 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Vac/Vac Membrane 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0.07 0.06 0.06 0.1 0 0.06 0.13 0.16 0.21
ensLOC DeepLoc
Localization WT1 WT2 WT3 RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3 WT1 WT2 WT3 AF100 AF140 AF180
Cortical Patches 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 1 0 0 0
Bud 1 0 0 1 0 2 10 2 8 10 12 8 0 0 0 1 1 1 0 2 1 1 1 1
Bud Neck 0 0 0 0 2 1 2 3 0 1 2 2 0 0 0 0 0 0 0 0 0 0 0 1
Bud Site 0 0 0 2 2 4 11 5 14 17 20 14 0 0 0 0 1 0
Cell Periphery 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 1 0 0 0 0 0 0 0
Cytoplasm 4 9 13 13 32 42 62 66 46 51 24 24 11 31 44 31 35 15 1 0 2 2 11 4
Endoplasmic Reticulum 1 0 0 0 0 0 1 0 0 0 0 0 0 2 1 8 5 2 0 0 0 0 0 2
Endosome 0 0 2 0 3 1 3 0 0 0 0 0 0 0 2 0 2 2 2 1 1 0 2 2
Golgi 0 0 0 0 0 0 0 0 0 0 0 0 0 0 1 3 6 4 0 1 0 0 2 1
Mitochondria 121 4 4 25 32 134 307 216 295 298 294 232 3 0 0 6 10 8 4 2 0 0 7 12
Nucleus 186 197 146 274 327 295 312 385 352 399 263 305 182 165 189 286 280 207 176 192 144 105 189 188
Nuclear Periphery 0 0 0 0 0 1 2 3 3 1 0 0 0 0 0 5 8 4 0 0 0 0 0 2
Nucleolus 0 0 0 1 0 1 3 2 1 3 1 1 0 0 0 1 5 5 0 0 0 0 11 10
Peroxisomes 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
SpindlePole 0 0 2 0 10 3 9 14 4 7 3 6 2 2 2 2 2 0 1 0 1 1 8 6
Vac/Vac Membrane 3 4 2 4 14 16 11 13 18 15 4 12 0 2 3 25 20 15 20 8 9 17 46 63
Unique Cell Count 212 209 163 292 391 402 529 576 518 575 403 432 195 201 237 351 344 244 210 214 168 132 287 302
Labelled Cell Count 316 214 169 320 422 500 733 709 741 802 623 604 198 202 242 368 376 263 210 214 168 132 287 302


Cytoplasm, Nucleus

(Huh et al. Nature 2003 425:686)
Screen WT1 WT2 WT3 RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3 AF100 AF140 AF180
Mean Cell GFP Intensity (1e-4) 3.6 5.5 5.9 5.1 5.4 4.2 3.8 4.7 3.7 4.0 3.5 4.0 6.0 6.2 5.7 10.0 10.2 9.6 5.5 5.9 6.1
Std Deviation (1e-4) 0.4 0.9 1.4 1.0 1.5 1.2 1.4 1.7 1.1 0.9 1.1 1.2 1.1 1.4 1.4 2.4 2.4 2.2 1.6 1.7 1.7
Intensity Change (Log2) -0.22 -0.14 -0.48 -0.62 -0.33 -0.68 -0.56 -0.74 -0.56 0.02 0.06 -0.04 0.75 0.79 0.7 -0.1 0.0 0.05


Localization RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3
Cortical Patches 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Bud 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Bud Neck 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Bud Site 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Cell Periphery 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Cytoplasm -1.6 0.1 0.9 1.3 1.3 0.4 0.4 -0.9 -1.1 -0.9 2.2 3.0 0.3 0.8 -0.7
Endoplasmic Reticulum 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Endosome 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Golgi 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Mitochondria 2.6 2.5 0 0 0 0 0 0 0 0 0 0 0 0 0
Nucleus 1.6 -1.8 -4.2 -7.2 -5.7 -5.4 -5.2 -5.8 -4.8 1.3 -2.0 -2.6 -2.3 -2.3 -1.4
Nuclear Periphery 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Nucleolus 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Peroxisomes 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
SpindlePole 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Vacuole 0 0 1.7 0 0 0 0 0 0 0 0 0 2.8 2.4 2.4

Images

Circle plot not available

Protein Concentration and Protein Localization Data

R1 R2 R3
G1 Pre-START G1 Post-START S/G2 Metaphase Anaphase Telophase G1 Pre-START G1 Post-START S/G2 Metaphase Anaphase Telophase G1 Pre-START G1 Post-START S/G2 Metaphase Anaphase Telophase
Concentration 1.8989 1.8515 1.6785 1.7354 1.2974 1.8404 1.8104 2.0228 1.714 1.3612 1.8255 1.8265 0.9069 1.0985 0.7828 0.7999 0.8412 0.573
Actin 0.0565 0 0.0053 0 0.0274 0.0107 0.0272 0 0.0051 0.003 0.0098 0.0004 0.0027 0 0.0097 0 0.0273 0.0066
Bud 0.0007 0 0.0003 0 0.0013 0.0004 0.0012 0 0.0007 0.0001 0.0001 0.0001 0.0003 0 0.0002 0 0.0007 0
Bud Neck 0.0017 0.0001 0.0005 0 0.0023 0.001 0.0022 0.0001 0.0024 0.0012 0.0005 0.0018 0.0013 0 0.0042 0 0.0022 0.0008
Bud Periphery 0.001 0 0.0004 0 0.0033 0.0006 0.003 0 0.0013 0.0001 0.0004 0.0006 0.0008 0 0.0003 0 0.0013 0
Bud Site 0.0157 0 0.0017 0 0.0045 0.0014 0.0067 0 0.0046 0.0003 0.0006 0.0001 0.001 0 0.0011 0 0.0024 0
Cell Periphery 0.0005 0 0.0001 0 0.001 0.0001 0.0006 0 0.0003 0 0 0.0002 0.0003 0 0.0001 0 0.0003 0
Cytoplasm 0.0025 0.0002 0.0017 0.0001 0.0009 0.0007 0.0041 0.0045 0.002 0.0002 0.0003 0.0002 0.0037 0.0003 0.0018 0.0002 0.0009 0.0001
Cytoplasmic Foci 0.0208 0 0.0095 0 0.009 0.0037 0.0127 0 0.0027 0.0029 0.0042 0.0018 0.006 0 0.0011 0 0.0282 0.0005
Eisosomes 0.0005 0 0 0 0.0003 0.0002 0.0005 0 0.0001 0.0001 0.0002 0 0.0002 0 0.0001 0 0.0002 0
Endoplasmic Reticulum 0.0046 0 0.0008 0 0.0018 0.001 0.0022 0 0.001 0.0001 0.0005 0.0004 0.0024 0 0.0001 0 0.0034 0
Endosome 0.0187 0 0.0125 0 0.0304 0.0143 0.035 0 0.0063 0.0016 0.0053 0.0059 0.0099 0 0.0013 0 0.0239 0.0001
Golgi 0.0087 0 0.0075 0 0.0256 0.0106 0.0115 0 0.0026 0.0019 0.0071 0.0021 0.0012 0 0.0018 0 0.02 0.0003
Lipid Particles 0.018 0 0.0038 0 0.0242 0.0075 0.0233 0 0.0059 0.0166 0.042 0.0034 0.0077 0 0.0021 0 0.0327 0.0002
Mitochondria 0.006 0.0004 0.012 0.0001 0.0189 0.01 0.0126 0.0001 0.0023 0.002 0.0026 0.0048 0.0106 0.0001 0.0095 0.0001 0.0062 0.0001
None 0.0012 0 0.0003 0 0.0003 0.0004 0.0045 0.0058 0.0006 0.0002 0.0005 0.0002 0.0013 0 0.0001 0 0.0007 0
Nuclear Periphery 0.0087 0.0018 0.005 0.0006 0.002 0.0027 0.0288 0.0037 0.0034 0.0194 0.0053 0.0028 0.0281 0.0019 0.007 0.002 0.0288 0.0007
Nucleolus 0.0076 0.0145 0.0044 0.0037 0.014 0.0165 0.0125 0.0053 0.0073 0.0041 0.0134 0.0163 0.0174 0.0062 0.0067 0.0039 0.0111 0.0148
Nucleus 0.7966 0.9821 0.9197 0.9953 0.8145 0.9093 0.7785 0.9786 0.9406 0.9355 0.8909 0.9463 0.8823 0.9909 0.949 0.9929 0.7707 0.9743
Peroxisomes 0.024 0 0.0065 0 0.0056 0.0025 0.0114 0 0.0029 0.008 0.0099 0.0024 0.0023 0 0.0028 0 0.0249 0.0006
Punctate Nuclear 0.0032 0.0005 0.0057 0.0002 0.001 0.004 0.0103 0.0018 0.0033 0.0021 0.0057 0.0084 0.0146 0.0002 0.0004 0.0008 0.0019 0.0006
Vacuole 0.0018 0.0001 0.0017 0 0.0097 0.0017 0.007 0 0.0037 0.0002 0.0002 0.0011 0.0029 0.0002 0.0004 0 0.0067 0.0001
Vacuole Periphery 0.001 0 0.0005 0 0.0021 0.0008 0.0044 0 0.0009 0.0004 0.0006 0.0007 0.003 0.0001 0.0003 0 0.0054 0

Sequencing Data

R1 R2
G1 Post-START S/G2 Metaphase Anaphase Telophase G1 Post-START S/G2 Metaphase Anaphase Telophase
Gene Expression 11.5251 8.9589 13.9606 10.1292 11.0447 2.0033 8.379 8.5593 9.574 8.2586
Translational Efficiency 2.3845 2.5406 1.4928 1.8176 1.8998 9.0307 1.5574 1.2759 1.337 1.9122

Hit Data

Dataset Hit
Protein Concentration
Protein Localization
Gene Expression
Translational Efficiency
Standard name
Human Ortholog
Description Essential protein required for the accumulation of box C/D snoRNA
Localization
Cell Percentages nucleus (97%)
Cell Cycle Regulation No
Subcompartmental Group nucleus-3

Bcd1

Bcd1


Nearest neighbours ( genes)

correlation threshold

0.6

1.0

0.80


Biological Process (Gene Ontology)

Molecular Function (Gene Ontology)

Cellular Component (Gene Ontology)


Endocytosis
Temp Actin Patch (Sac6-tdTomato) Cortical Patch (Sla1-GFP) Late Endosome (Snf7-GFP) Vacuole (Vph1-GFP)
37℃
RT
Cell Cycle Omics

CYCLoPs (Bcd1-GFP)
Gene / Allele Actin Patch (Sac6-tdTomato) Cortical Patch (Sla1-GFP) Late Endosome (Snf7-GFP) Vacuole (Sac6-tdTomato)
Gene Images
Gene Images
Images are not yet available
Images are not yet available