Standard name
Description Essential protein required for the accumulation of box C/D snoRNA
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Micrographs

ensLOC DeepLoc
Localization WT1 WT2 WT3 RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3 WT1 WT2 WT3 AF100 AF140 AF180
Cortical Patches 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Bud 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Bud Neck 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Bud Site 0 0 0 0 0 0 0 0 0 0 0.05 0 0 0 0 0 0 0 – – – – – –
Cell Periphery 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Cytoplasm 0 0 0.08 0 0.08 0.1 0.12 0.11 0.09 0.09 0.06 0.06 0.06 0.15 0.19 0.09 0.1 0.06 0 0 0 0 0 0
Endoplasmic Reticulum 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Endosome 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Golgi 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Mitochondria 0.57 0 0 0.09 0.08 0.33 0.58 0.38 0.57 0.52 0.73 0.54 0 0 0 0 0 0 0 0 0 0 0 0
Nucleus 0.88 0.94 0.9 0.94 0.84 0.73 0.59 0.67 0.68 0.69 0.65 0.71 0.93 0.82 0.8 0.81 0.81 0.85 0.84 0.9 0.86 0.8 0.66 0.62
Nuclear Periphery 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Nucleolus 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Peroxisomes 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
SpindlePole 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Vac/Vac Membrane 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0.07 0.06 0.06 0.1 0 0.06 0.13 0.16 0.21
ensLOC DeepLoc
Localization WT1 WT2 WT3 RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3 WT1 WT2 WT3 AF100 AF140 AF180
Cortical Patches 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 1 0 0 0
Bud 1 0 0 1 0 2 10 2 8 10 12 8 0 0 0 1 1 1 0 2 1 1 1 1
Bud Neck 0 0 0 0 2 1 2 3 0 1 2 2 0 0 0 0 0 0 0 0 0 0 0 1
Bud Site 0 0 0 2 2 4 11 5 14 17 20 14 0 0 0 0 1 0 – – – – – –
Cell Periphery 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 1 0 0 0 0 0 0 0
Cytoplasm 4 9 13 13 32 42 62 66 46 51 24 24 11 31 44 31 35 15 1 0 2 2 11 4
Endoplasmic Reticulum 1 0 0 0 0 0 1 0 0 0 0 0 0 2 1 8 5 2 0 0 0 0 0 2
Endosome 0 0 2 0 3 1 3 0 0 0 0 0 0 0 2 0 2 2 2 1 1 0 2 2
Golgi 0 0 0 0 0 0 0 0 0 0 0 0 0 0 1 3 6 4 0 1 0 0 2 1
Mitochondria 121 4 4 25 32 134 307 216 295 298 294 232 3 0 0 6 10 8 4 2 0 0 7 12
Nucleus 186 197 146 274 327 295 312 385 352 399 263 305 182 165 189 286 280 207 176 192 144 105 189 188
Nuclear Periphery 0 0 0 0 0 1 2 3 3 1 0 0 0 0 0 5 8 4 0 0 0 0 0 2
Nucleolus 0 0 0 1 0 1 3 2 1 3 1 1 0 0 0 1 5 5 0 0 0 0 11 10
Peroxisomes 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
SpindlePole 0 0 2 0 10 3 9 14 4 7 3 6 2 2 2 2 2 0 1 0 1 1 8 6
Vac/Vac Membrane 3 4 2 4 14 16 11 13 18 15 4 12 0 2 3 25 20 15 20 8 9 17 46 63
Unique Cell Count 212 209 163 292 391 402 529 576 518 575 403 432 195 201 237 351 344 244 210 214 168 132 287 302
Labelled Cell Count 316 214 169 320 422 500 733 709 741 802 623 604 198 202 242 368 376 263 210 214 168 132 287 302


Cytoplasm, Nucleus

(Huh et al. Nature 2003 425:686)
Screen WT1 WT2 WT3 RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3 AF100 AF140 AF180
Mean Cell GFP Intensity (1e-4) 3.6 5.5 5.9 5.1 5.4 4.2 3.8 4.7 3.7 4.0 3.5 4.0 6.0 6.2 5.7 10.0 10.2 9.6 5.5 5.9 6.1
Std Deviation (1e-4) 0.4 0.9 1.4 1.0 1.5 1.2 1.4 1.7 1.1 0.9 1.1 1.2 1.1 1.4 1.4 2.4 2.4 2.2 1.6 1.7 1.7
Intensity Change (Log2) – – – -0.22 -0.14 -0.48 -0.62 -0.33 -0.68 -0.56 -0.74 -0.56 0.02 0.06 -0.04 0.75 0.79 0.7 -0.1 0.0 0.05


Localization RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3
Cortical Patches 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Bud 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Bud Neck 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Bud Site 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Cell Periphery 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Cytoplasm -1.6 0.1 0.9 1.3 1.3 0.4 0.4 -0.9 -1.1 -0.9 2.2 3.0 0.3 0.8 -0.7
Endoplasmic Reticulum 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Endosome 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Golgi 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Mitochondria 2.6 2.5 0 0 0 0 0 0 0 0 0 0 0 0 0
Nucleus 1.6 -1.8 -4.2 -7.2 -5.7 -5.4 -5.2 -5.8 -4.8 1.3 -2.0 -2.6 -2.3 -2.3 -1.4
Nuclear Periphery 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Nucleolus 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Peroxisomes 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
SpindlePole 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Vacuole 0 0 1.7 0 0 0 0 0 0 0 0 0 2.8 2.4 2.4