Standard name | |
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Human Ortholog | |
Description | Essential protein required for the accumulation of box C/D snoRNA |
ensLOC | DeepLoc | |||||||||||||||||||||||
---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|
Localization | WT1 | WT2 | WT3 | RAP60 | RAP140 | RAP220 | RAP300 | RAP380 | RAP460 | RAP540 | RAP620 | RAP700 | HU80 | HU120 | HU160 | rpd3Δ_1 | rpd3Δ_2 | rpd3Δ_3 | WT1 | WT2 | WT3 | AF100 | AF140 | AF180 |
Cortical Patches | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 1 | 0 | 0 | 0 |
Bud | 1 | 0 | 0 | 1 | 0 | 2 | 10 | 2 | 8 | 10 | 12 | 8 | 0 | 0 | 0 | 1 | 1 | 1 | 0 | 2 | 1 | 1 | 1 | 1 |
Bud Neck | 0 | 0 | 0 | 0 | 2 | 1 | 2 | 3 | 0 | 1 | 2 | 2 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 1 |
Bud Site | 0 | 0 | 0 | 2 | 2 | 4 | 11 | 5 | 14 | 17 | 20 | 14 | 0 | 0 | 0 | 0 | 1 | 0 | – | – | – | – | – | – |
Cell Periphery | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 1 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
Cytoplasm | 4 | 9 | 13 | 13 | 32 | 42 | 62 | 66 | 46 | 51 | 24 | 24 | 11 | 31 | 44 | 31 | 35 | 15 | 1 | 0 | 2 | 2 | 11 | 4 |
Endoplasmic Reticulum | 1 | 0 | 0 | 0 | 0 | 0 | 1 | 0 | 0 | 0 | 0 | 0 | 0 | 2 | 1 | 8 | 5 | 2 | 0 | 0 | 0 | 0 | 0 | 2 |
Endosome | 0 | 0 | 2 | 0 | 3 | 1 | 3 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 2 | 0 | 2 | 2 | 2 | 1 | 1 | 0 | 2 | 2 |
Golgi | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 1 | 3 | 6 | 4 | 0 | 1 | 0 | 0 | 2 | 1 |
Mitochondria | 121 | 4 | 4 | 25 | 32 | 134 | 307 | 216 | 295 | 298 | 294 | 232 | 3 | 0 | 0 | 6 | 10 | 8 | 4 | 2 | 0 | 0 | 7 | 12 |
Nucleus | 186 | 197 | 146 | 274 | 327 | 295 | 312 | 385 | 352 | 399 | 263 | 305 | 182 | 165 | 189 | 286 | 280 | 207 | 176 | 192 | 144 | 105 | 189 | 188 |
Nuclear Periphery | 0 | 0 | 0 | 0 | 0 | 1 | 2 | 3 | 3 | 1 | 0 | 0 | 0 | 0 | 0 | 5 | 8 | 4 | 0 | 0 | 0 | 0 | 0 | 2 |
Nucleolus | 0 | 0 | 0 | 1 | 0 | 1 | 3 | 2 | 1 | 3 | 1 | 1 | 0 | 0 | 0 | 1 | 5 | 5 | 0 | 0 | 0 | 0 | 11 | 10 |
Peroxisomes | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
SpindlePole | 0 | 0 | 2 | 0 | 10 | 3 | 9 | 14 | 4 | 7 | 3 | 6 | 2 | 2 | 2 | 2 | 2 | 0 | 1 | 0 | 1 | 1 | 8 | 6 |
Vac/Vac Membrane | 3 | 4 | 2 | 4 | 14 | 16 | 11 | 13 | 18 | 15 | 4 | 12 | 0 | 2 | 3 | 25 | 20 | 15 | 20 | 8 | 9 | 17 | 46 | 63 |
Unique Cell Count | 212 | 209 | 163 | 292 | 391 | 402 | 529 | 576 | 518 | 575 | 403 | 432 | 195 | 201 | 237 | 351 | 344 | 244 | 210 | 214 | 168 | 132 | 287 | 302 |
Labelled Cell Count | 316 | 214 | 169 | 320 | 422 | 500 | 733 | 709 | 741 | 802 | 623 | 604 | 198 | 202 | 242 | 368 | 376 | 263 | 210 | 214 | 168 | 132 | 287 | 302 |
Screen | WT1 | WT2 | WT3 | RAP60 | RAP140 | RAP220 | RAP300 | RAP380 | RAP460 | RAP540 | RAP620 | RAP700 | HU80 | HU120 | HU160 | rpd3Δ_1 | rpd3Δ_2 | rpd3Δ_3 | AF100 | AF140 | AF180 |
---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|
Mean Cell GFP Intensity (1e-4) | 3.6 | 5.5 | 5.9 | 5.1 | 5.4 | 4.2 | 3.8 | 4.7 | 3.7 | 4.0 | 3.5 | 4.0 | 6.0 | 6.2 | 5.7 | 10.0 | 10.2 | 9.6 | 5.5 | 5.9 | 6.1 |
Std Deviation (1e-4) | 0.4 | 0.9 | 1.4 | 1.0 | 1.5 | 1.2 | 1.4 | 1.7 | 1.1 | 0.9 | 1.1 | 1.2 | 1.1 | 1.4 | 1.4 | 2.4 | 2.4 | 2.2 | 1.6 | 1.7 | 1.7 |
Intensity Change (Log2) | – | – | – | -0.22 | -0.14 | -0.48 | -0.62 | -0.33 | -0.68 | -0.56 | -0.74 | -0.56 | 0.02 | 0.06 | -0.04 | 0.75 | 0.79 | 0.7 | -0.1 | 0.0 | 0.05 |
Localization | RAP60 | RAP140 | RAP220 | RAP300 | RAP380 | RAP460 | RAP540 | RAP620 | RAP700 | HU80 | HU120 | HU160 | rpd3Δ_1 | rpd3Δ_2 | rpd3Δ_3 |
---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|
Cortical Patches | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
Bud | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
Bud Neck | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
Bud Site | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
Cell Periphery | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
Cytoplasm | -1.6 | 0.1 | 0.9 | 1.3 | 1.3 | 0.4 | 0.4 | -0.9 | -1.1 | -0.9 | 2.2 | 3.0 | 0.3 | 0.8 | -0.7 |
Endoplasmic Reticulum | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
Endosome | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
Golgi | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
Mitochondria | 2.6 | 2.5 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
Nucleus | 1.6 | -1.8 | -4.2 | -7.2 | -5.7 | -5.4 | -5.2 | -5.8 | -4.8 | 1.3 | -2.0 | -2.6 | -2.3 | -2.3 | -1.4 |
Nuclear Periphery | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
Nucleolus | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
Peroxisomes | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
SpindlePole | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
Vacuole | 0 | 0 | 1.7 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 2.8 | 2.4 | 2.4 |
R1 | R2 | R3 | ||||||||||||||||
---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|
G1 Pre-START | G1 Post-START | S/G2 | Metaphase | Anaphase | Telophase | G1 Pre-START | G1 Post-START | S/G2 | Metaphase | Anaphase | Telophase | G1 Pre-START | G1 Post-START | S/G2 | Metaphase | Anaphase | Telophase | |
Concentration | 1.8989 | 1.8515 | 1.6785 | 1.7354 | 1.2974 | 1.8404 | 1.8104 | 2.0228 | 1.714 | 1.3612 | 1.8255 | 1.8265 | 0.9069 | 1.0985 | 0.7828 | 0.7999 | 0.8412 | 0.573 |
Actin | 0.0565 | 0 | 0.0053 | 0 | 0.0274 | 0.0107 | 0.0272 | 0 | 0.0051 | 0.003 | 0.0098 | 0.0004 | 0.0027 | 0 | 0.0097 | 0 | 0.0273 | 0.0066 |
Bud | 0.0007 | 0 | 0.0003 | 0 | 0.0013 | 0.0004 | 0.0012 | 0 | 0.0007 | 0.0001 | 0.0001 | 0.0001 | 0.0003 | 0 | 0.0002 | 0 | 0.0007 | 0 |
Bud Neck | 0.0017 | 0.0001 | 0.0005 | 0 | 0.0023 | 0.001 | 0.0022 | 0.0001 | 0.0024 | 0.0012 | 0.0005 | 0.0018 | 0.0013 | 0 | 0.0042 | 0 | 0.0022 | 0.0008 |
Bud Periphery | 0.001 | 0 | 0.0004 | 0 | 0.0033 | 0.0006 | 0.003 | 0 | 0.0013 | 0.0001 | 0.0004 | 0.0006 | 0.0008 | 0 | 0.0003 | 0 | 0.0013 | 0 |
Bud Site | 0.0157 | 0 | 0.0017 | 0 | 0.0045 | 0.0014 | 0.0067 | 0 | 0.0046 | 0.0003 | 0.0006 | 0.0001 | 0.001 | 0 | 0.0011 | 0 | 0.0024 | 0 |
Cell Periphery | 0.0005 | 0 | 0.0001 | 0 | 0.001 | 0.0001 | 0.0006 | 0 | 0.0003 | 0 | 0 | 0.0002 | 0.0003 | 0 | 0.0001 | 0 | 0.0003 | 0 |
Cytoplasm | 0.0025 | 0.0002 | 0.0017 | 0.0001 | 0.0009 | 0.0007 | 0.0041 | 0.0045 | 0.002 | 0.0002 | 0.0003 | 0.0002 | 0.0037 | 0.0003 | 0.0018 | 0.0002 | 0.0009 | 0.0001 |
Cytoplasmic Foci | 0.0208 | 0 | 0.0095 | 0 | 0.009 | 0.0037 | 0.0127 | 0 | 0.0027 | 0.0029 | 0.0042 | 0.0018 | 0.006 | 0 | 0.0011 | 0 | 0.0282 | 0.0005 |
Eisosomes | 0.0005 | 0 | 0 | 0 | 0.0003 | 0.0002 | 0.0005 | 0 | 0.0001 | 0.0001 | 0.0002 | 0 | 0.0002 | 0 | 0.0001 | 0 | 0.0002 | 0 |
Endoplasmic Reticulum | 0.0046 | 0 | 0.0008 | 0 | 0.0018 | 0.001 | 0.0022 | 0 | 0.001 | 0.0001 | 0.0005 | 0.0004 | 0.0024 | 0 | 0.0001 | 0 | 0.0034 | 0 |
Endosome | 0.0187 | 0 | 0.0125 | 0 | 0.0304 | 0.0143 | 0.035 | 0 | 0.0063 | 0.0016 | 0.0053 | 0.0059 | 0.0099 | 0 | 0.0013 | 0 | 0.0239 | 0.0001 |
Golgi | 0.0087 | 0 | 0.0075 | 0 | 0.0256 | 0.0106 | 0.0115 | 0 | 0.0026 | 0.0019 | 0.0071 | 0.0021 | 0.0012 | 0 | 0.0018 | 0 | 0.02 | 0.0003 |
Lipid Particles | 0.018 | 0 | 0.0038 | 0 | 0.0242 | 0.0075 | 0.0233 | 0 | 0.0059 | 0.0166 | 0.042 | 0.0034 | 0.0077 | 0 | 0.0021 | 0 | 0.0327 | 0.0002 |
Mitochondria | 0.006 | 0.0004 | 0.012 | 0.0001 | 0.0189 | 0.01 | 0.0126 | 0.0001 | 0.0023 | 0.002 | 0.0026 | 0.0048 | 0.0106 | 0.0001 | 0.0095 | 0.0001 | 0.0062 | 0.0001 |
None | 0.0012 | 0 | 0.0003 | 0 | 0.0003 | 0.0004 | 0.0045 | 0.0058 | 0.0006 | 0.0002 | 0.0005 | 0.0002 | 0.0013 | 0 | 0.0001 | 0 | 0.0007 | 0 |
Nuclear Periphery | 0.0087 | 0.0018 | 0.005 | 0.0006 | 0.002 | 0.0027 | 0.0288 | 0.0037 | 0.0034 | 0.0194 | 0.0053 | 0.0028 | 0.0281 | 0.0019 | 0.007 | 0.002 | 0.0288 | 0.0007 |
Nucleolus | 0.0076 | 0.0145 | 0.0044 | 0.0037 | 0.014 | 0.0165 | 0.0125 | 0.0053 | 0.0073 | 0.0041 | 0.0134 | 0.0163 | 0.0174 | 0.0062 | 0.0067 | 0.0039 | 0.0111 | 0.0148 |
Nucleus | 0.7966 | 0.9821 | 0.9197 | 0.9953 | 0.8145 | 0.9093 | 0.7785 | 0.9786 | 0.9406 | 0.9355 | 0.8909 | 0.9463 | 0.8823 | 0.9909 | 0.949 | 0.9929 | 0.7707 | 0.9743 |
Peroxisomes | 0.024 | 0 | 0.0065 | 0 | 0.0056 | 0.0025 | 0.0114 | 0 | 0.0029 | 0.008 | 0.0099 | 0.0024 | 0.0023 | 0 | 0.0028 | 0 | 0.0249 | 0.0006 |
Punctate Nuclear | 0.0032 | 0.0005 | 0.0057 | 0.0002 | 0.001 | 0.004 | 0.0103 | 0.0018 | 0.0033 | 0.0021 | 0.0057 | 0.0084 | 0.0146 | 0.0002 | 0.0004 | 0.0008 | 0.0019 | 0.0006 |
Vacuole | 0.0018 | 0.0001 | 0.0017 | 0 | 0.0097 | 0.0017 | 0.007 | 0 | 0.0037 | 0.0002 | 0.0002 | 0.0011 | 0.0029 | 0.0002 | 0.0004 | 0 | 0.0067 | 0.0001 |
Vacuole Periphery | 0.001 | 0 | 0.0005 | 0 | 0.0021 | 0.0008 | 0.0044 | 0 | 0.0009 | 0.0004 | 0.0006 | 0.0007 | 0.003 | 0.0001 | 0.0003 | 0 | 0.0054 | 0 |
R1 | R2 | |||||||||
---|---|---|---|---|---|---|---|---|---|---|
G1 Post-START | S/G2 | Metaphase | Anaphase | Telophase | G1 Post-START | S/G2 | Metaphase | Anaphase | Telophase | |
Gene Expression | 11.5251 | 8.9589 | 13.9606 | 10.1292 | 11.0447 | 2.0033 | 8.379 | 8.5593 | 9.574 | 8.2586 |
Translational Efficiency | 2.3845 | 2.5406 | 1.4928 | 1.8176 | 1.8998 | 9.0307 | 1.5574 | 1.2759 | 1.337 | 1.9122 |
Dataset | Hit |
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Protein Concentration | ✘ |
Protein Localization | ✘ |
Gene Expression | ✘ |
Translational Efficiency | ✘ |
Temp | Actin Patch (Sac6-tdTomato) | Cortical Patch (Sla1-GFP) | Late Endosome (Snf7-GFP) | Vacuole (Vph1-GFP) |
---|---|---|---|---|
37℃ | ||||
RT |
Gene / Allele | Actin Patch (Sac6-tdTomato) | Cortical Patch (Sla1-GFP) | Late Endosome (Snf7-GFP) | Vacuole (Sac6-tdTomato) |
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Gene | Images |
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Gene | Images |
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