Standard name
Description NADP-cytochrome P450 reductase; involved in ergosterol biosynthesis; associated and coordinately regulated with Erg11p

Micrographs

Sub-cellular Localization

ensLOC DeepLoc
Localization WT1 WT2 WT3 RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3 WT1 WT2 WT3 AF100 AF140 AF180
Cortical Patches 0.06 0.06 0.07 0.12 0.07 0.06 0.06 0.05 0.08 0.08 0.09 0 0 0 0.18 0.10 0.06 0 0 0 0 0 0
Bud 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0.05 0 0 0 0 0 0 0
Bud Neck 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Bud Site 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Cell Periphery 0.08 0.10 0.10 0.12 0.08 0.12 0.08 0 0.08 0.08 0.12 0.19 0.19 0.26 0.20 0.09 0.36 0 0 0 0 0 0.06
Cytoplasm 0.07 0.14 0.07 0.18 0.16 0.14 0.12 0.13 0.21 0.18 0.15 0.11 0.11 0.12 0.48 0.69 0.44 0 0 0 0 0 0
Endoplasmic Reticulum 0.91 0.84 0.92 0.78 0.77 0.82 0.78 0.79 0.61 0.62 0.71 0.84 0.85 0.79 0 0 0 0.95 0.90 0.93 0.93 0.90 0.86
Endosome 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Golgi 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0.10 0 0 0 0 0 0
Mitochondria 0 0 0 0 0 0.05 0 0.06 0.15 0.20 0.10 0 0 0 0.09 0.08 0.11 0 0 0 0 0 0
Nucleus 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Nuclear Periphery 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Nucleolus 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Peroxisomes 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
SpindlePole 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Vac/Vac Membrane 0 0 0 0 0.09 0 0.08 0.05 0.05 0 0 0 0 0 0.09 0 0 0 0 0 0 0 0
ensLOC DeepLoc
Localization WT1 WT2 WT3 RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3 WT1 WT2 WT3 AF100 AF140 AF180
Cortical Patches 13 14 25 25 27 26 46 37 38 40 58 7 8 9 29 16 6 0 5 6 4 5 9
Bud 3 1 3 1 3 2 5 4 6 4 8 1 1 1 6 8 1 0 0 0 0 0 1
Bud Neck 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 1 0 0 0 1
Bud Site 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Cell Periphery 19 25 38 25 30 55 59 25 36 40 81 62 62 77 32 14 37 3 5 9 3 14 24
Cytoplasm 16 35 25 38 61 64 92 92 96 94 102 38 38 34 78 108 45 2 5 1 2 4 4
Endoplasmic Reticulum 210 204 336 170 299 363 578 550 281 321 482 279 283 232 1 4 2 223 231 356 186 308 375
Endosome 0 0 0 0 1 0 0 0 2 0 0 0 0 0 2 0 1 0 0 1 0 0 1
Golgi 1 0 2 8 11 1 9 1 1 2 5 1 1 0 3 4 10 1 1 1 0 2 2
Mitochondria 2 0 4 5 11 21 31 41 70 101 71 0 0 0 14 12 11 0 2 2 1 1 4
Nucleus 0 0 1 0 1 0 0 1 0 1 0 0 0 0 2 1 1 0 0 0 0 0 0
Nuclear Periphery 3 0 6 2 1 11 14 24 17 15 21 0 0 0 0 2 0 0 0 1 0 1 0
Nucleolus 1 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Peroxisomes 0 0 0 0 0 0 0 1 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
SpindlePole 0 0 0 0 1 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Vac/Vac Membrane 3 1 3 2 36 13 57 33 23 16 19 2 2 2 14 7 1 0 5 1 1 0 7
Unique Cell Count 232 243 367 217 390 445 740 700 459 515 680 334 332 295 163 157 103 235 257 385 201 342 437
Labelled Cell Count 271 280 443 276 482 556 891 809 570 634 847 390 395 355 181 176 115 235 257 385 201 342 437

Yeast GFP Assignment

(Huh et al. Nature 2003 425:686 )

Endoplasmic Reticulum

Protein Abundance

Screen WT1 WT2 WT3 RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3 AF100 AF140 AF180
Mean Cell GFP Intensity (1e-4) 16.3 18.1 14.9 14.9 14.4 13.2 13.3 12.8 11.7 11.5 12.5 19.9 19.9 21.6 11.9 14.2 19.2 19.2 19.3 19.6
Std Deviation (1e-4) 2.8 3.2 2.7 2.8 2.5 2.2 2.4 2.3 2.5 2.4 2.4 3.4 3.4 4.0 2.8 3.9 5.3 2.9 3.2 3.4
Intensity Change (Log2) -0.0 -0.05 -0.17 -0.16 -0.21 -0.35 -0.37 -0.26 0.41 0.41 0.54 -0.33 -0.07 0.36 0.36 0.38 0.39

Localization Change

Localization RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3
Cortical Patches 2.0 0.1 -0.6 -0.4 -1.0 0.8 0.5 1.0 -3.0 -2.7 -2.2 3.9 1.3 -0.4
Bud 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Bud Neck 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Bud Site 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Cell Periphery 0.4 -1.3 0.9 -1.3 -4.5 -1.3 -1.3 0.8 3.1 3.1 5.3 2.9 -0.5 6.3
Cytoplasm 3.1 2.1 2.6 1.6 1.9 4.3 4.0 2.9 0.6 0.7 1.1 7.3 10.1 6.0
Endoplasmic Reticulum -4.5 -5.6 -4.1 -5.6 -5.4 -10.0 -9.8 -7.7 -3.2 -2.6 -4.7 -20.1 -19.6 -17.9
Endosome 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Golgi 0 2.4 0 0 0 0 0 0 0 0 0 0 0 0
Mitochondria 0 1.7 3.0 2.8 3.7 7.1 8.4 5.6 0 0 0 4.4 0 0
Nucleus 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Nuclear Periphery 0 0 0.8 0.3 1.7 1.8 1.2 1.4 0 0 0 0 0 0
Nucleolus 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Peroxisomes 0 0 0 0 0 0 0 0 0 0 0 0 0 0
SpindlePole 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Vacuole 0 0 0 0 0 0 0 0 0 0 0 0 0 0