Standard name
Description Lanosterol synthase; an essential enzyme that catalyzes the cyclization of squalene 2,3-epoxide, a step in ergosterol biosynthesis; human LSS functionally complements the lethality of the erg7 null mutation

Micrographs

ensLOC DeepLoc
Localization WT1 WT2 WT3 RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3 WT1 WT2 WT3 AF100 AF140 AF180
Cortical Patches 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0.05 0 0 0 0.06
Bud 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Bud Neck 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Bud Site 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Cell Periphery 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Cytoplasm 0 0 0 0.05 0.05 0 0 0 0 0 0 0.05 0.05 0 0.15 0.24 0.27 0 0 0 0 0 0
Endoplasmic Reticulum 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0.05 0 0 0 0 0 0
Endosome 0.22 0.08 0.21 0.18 0.36 0.07 0.05 0 0 0 0 0.24 0.25 0.39 0.42 0.34 0.33 0.28 0.34 0.22 0.19 0.24 0.19
Golgi 0 0 0 0 0.05 0 0 0 0 0 0 0.06 0.06 0.06 0 0 0.13 0.17 0.17 0.09 0.11 0.12 0.13
Mitochondria 0.65 0.83 0.58 0.58 0.49 0.82 0.84 0.79 0.86 0.9 0.87 0.32 0.32 0.18 0.1 0.07 0.15 0 0.05 0 0 0 0.05
Nucleus 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0.05 0.1 0 0 0 0 0 0 0
Nuclear Periphery 0.08 0.08 0.05 0.05 0.05 0.07 0.09 0.09 0.12 0.11 0.1 0.06 0.07 0.05 0 0 0.05 0 0 0 0 0 0
Nucleolus 0.18 0.18 0.12 0.18 0.07 0.16 0.18 0.2 0.22 0.24 0.2 0.3 0.3 0.32 0.05 0.11 0.09 0 0 0 0 0 0
Peroxisomes 0.21 0.06 0.47 0.39 0.18 0.05 0.07 0.05 0 0 0 0.16 0.17 0.15 0.08 0 0.08 0.41 0.26 0.55 0.54 0.5 0.51
SpindlePole 0.08 0.08 0.11 0.12 0.06 0 0 0 0 0 0 0.2 0.21 0.22 0.31 0.32 0.1 0 0.07 0 0 0 0
Vac/Vac Membrane 0 0 0 0 0 0 0 0 0 0 0.05 0 0 0.08 0.09 0.05 0.12 0 0 0 0 0 0
ensLOC DeepLoc
Localization WT1 WT2 WT3 RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3 WT1 WT2 WT3 AF100 AF140 AF180
Cortical Patches 4 9 5 1 0 0 1 0 0 0 0 6 6 6 3 2 2 12 12 21 9 8 18
Bud 1 6 5 6 5 11 6 6 7 7 6 0 0 0 0 1 1 0 3 1 0 0 1
Bud Neck 0 0 0 0 0 0 0 0 0 0 0 0 0 0 1 0 0 0 0 1 0 1 0
Bud Site 0 1 1 1 1 0 0 4 3 4 1 0 0 0 0 0 0
Cell Periphery 0 0 0 0 0 0 1 1 0 0 0 1 1 0 1 0 0 0 0 0 0 0 0
Cytoplasm 1 5 20 18 21 13 16 16 14 9 6 11 11 9 35 33 29 0 1 1 0 1 0
Endoplasmic Reticulum 3 3 2 0 7 10 2 1 0 1 1 0 2 2 7 6 5 0 0 1 1 1 0
Endosome 57 18 101 72 155 37 40 32 0 3 8 50 54 86 99 47 35 79 81 111 42 71 59
Golgi 10 1 1 1 20 0 1 1 0 0 0 12 13 13 4 5 14 48 41 46 24 34 40
Mitochondria 173 192 282 230 210 447 654 626 577 504 527 67 67 39 24 10 16 12 12 12 9 9 13
Nucleus 0 1 0 0 0 3 6 7 7 7 8 2 2 3 11 14 3 0 0 0 0 1 0
Nuclear Periphery 22 18 24 18 20 39 67 73 83 59 60 13 15 11 5 6 5 1 3 4 1 3 4
Nucleolus 48 41 59 70 30 87 141 157 151 132 122 63 63 70 13 16 10 0 0 0 0 1 0
Peroxisomes 56 14 228 155 78 28 53 36 5 9 14 33 37 33 20 3 9 117 61 278 122 147 155
SpindlePole 22 19 52 46 26 20 13 23 4 9 8 42 45 49 73 45 11 11 16 16 8 9 7
Vac/Vac Membrane 4 1 2 0 10 11 19 22 27 16 33 2 1 18 22 7 13 1 3 1 2 2 1
Unique Cell Count 265 231 483 399 430 543 775 791 673 561 608 208 212 220 237 140 107 288 240 503 226 297 307
Labelled Cell Count 401 329 782 618 583 706 1020 1005 878 760 794 302 317 339 318 195 153 288 240 503 226 297 307


Lipid Particles

(Huh et al. Nature 2003 425:686)
Screen WT1 WT2 WT3 RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3 AF100 AF140 AF180
Mean Cell GFP Intensity (1e-4) 9.4 8.0 8.2 8.1 8.1 7.1 7.5 7.7 7.4 7.0 7.6 10.3 10.3 10.4 6.8 6.8 9.4 10.4 10.5 11.3
Std Deviation (1e-4) 1.5 2.1 1.8 1.5 2.1 1.6 1.6 1.8 1.8 2.0 1.8 2.0 2.0 1.7 2.0 1.7 2.3 2.0 2.1 2.2
Intensity Change (Log2) -0.03 -0.02 -0.21 -0.13 -0.09 -0.16 -0.24 -0.12 0.32 0.32 0.34 -0.28 -0.28 0.19 0.34 0.35 0.45


Localization RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3
Cortical Patches 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Bud 0 0 1.3 0 0 0 0.3 0 0 0 0 0 0 0
Bud Neck 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Bud Site 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Cell Periphery 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Cytoplasm 0.3 0.5 -1.6 -2.1 -2.2 -2.0 -2.5 -3.4 0.7 0.6 0 5.0 7.3 7.8
Endoplasmic Reticulum 0 0 2.1 0 0 0 0 0 0 0 0 0 0 0
Endosome -1.1 5.1 -6.6 -8.6 -9.6 -12.4 -11.0 -10.7 0.9 1.3 5.1 5.9 3.1 2.6
Golgi 0 4.5 0 0 0 0 0 0 0 0 0 0 0 0
Mitochondria -0.2 -2.9 8.4 10.3 7.9 10.5 11.7 10.6 -6.3 -6.5 -10.0 -12.3 -10.7 -8.1
Nucleus 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Nuclear Periphery -0.3 -0.2 1.5 2.4 2.8 4.3 3.3 3.0 0.7 1.1 0 -1.8 -0.3 -0.1
Nucleolus 2.2 -2.7 1.7 2.8 3.5 4.4 4.7 3.5 5.7 5.6 6.2 -2.8 -0.3 -0.8
Peroxisomes -2.5 -9.3 -15.5 -16.7 -18.2 -19.4 -17.5 -17.7 -7.8 -7.4 -8.2 -10.3 -9.7 -7.4
SpindlePole 0.4 -2.5 -4.4 -7.1 -5.8 -7.9 -6.3 -6.8 3.3 3.7 4.0 6.7 6.1 -0.1
Vacuole 0 2.5 2.3 2.7 3.0 3.9 3.0 4.7 0 0 5.7 6.2 0 0


Endocytosis
Temp Actin Patch (Sac6-tdTomato) Cortical Patch (Sla1-GFP) Late Endosome (Snf7-GFP) Vacuole (Vph1-GFP)
37℃
RT
Cell Cycle Omics

CYCLoPs (Erg7-GFP)
Gene / Allele Actin Patch (Sac6-tdTomato) Cortical Patch (Sla1-GFP) Late Endosome (Snf7-GFP) Vacuole (Sac6-tdTomato)
Gene Images
Gene Images
Images are not yet available
Images are not yet available