Standard name
Description Subunit of box H/ACA snoRNP complex; required for pseudouridylation and processing of pre-18S rRNA

Micrographs

ensLOC DeepLoc
Localization WT1 WT2 WT3 RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3 WT1 WT2 WT3 AF100 AF140 AF180
Cortical Patches 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Bud 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Bud Neck 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Bud Site 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Cell Periphery 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Cytoplasm 0 0 0 0 0 0 0 0 0 0 0 0.05 0.09 0.12 0 0 0 0 0 0 0 0 0
Endoplasmic Reticulum 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Endosome 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Golgi 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Mitochondria 0.4 0.36 0.58 0.29 0.43 0.35 0.3 0.41 0.31 0.34 0.37 0.38 0.41 0.31 0.12 0.06 0.05 0 0 0 0 0 0
Nucleus 0.13 0.14 0.06 0 0 0 0.05 0 0.06 0 0 0.12 0.1 0.09 0.06 0.07 0.07 0.14 0.13 0.09 0.08 0 0.07
Nuclear Periphery 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Nucleolus 0.95 0.9 0.98 0.95 0.94 0.92 0.91 0.94 0.93 0.94 0.91 0.88 0.83 0.8 0.91 0.93 0.91 0.8 0.8 0.86 0.87 0.87 0.81
Peroxisomes 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
SpindlePole 0 0 0 0 0 0.05 0 0 0.06 0 0.06 0 0 0 0 0 0 0 0 0 0 0 0
Vac/Vac Membrane 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
ensLOC DeepLoc
Localization WT1 WT2 WT3 RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3 WT1 WT2 WT3 AF100 AF140 AF180
Cortical Patches 0 0 0 0 0 0 0 0 0 0 0 0 0 1 0 0 1 0 0 0 0 0 0
Bud 0 0 1 0 0 1 1 0 0 0 3 0 1 2 0 0 1 1 0 1 0 2 2
Bud Neck 1 5 0 0 2 4 2 3 3 1 5 1 0 0 1 0 0 1 0 0 0 2 2
Bud Site 3 2 0 0 2 4 6 8 12 7 14 0 0 1 0 0 0
Cell Periphery 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Cytoplasm 1 1 4 6 4 4 5 4 4 1 4 11 26 51 13 12 18 0 0 0 0 0 1
Endoplasmic Reticulum 0 1 0 0 0 0 0 0 0 0 0 0 0 0 2 0 7 0 0 0 0 0 1
Endosome 0 1 0 1 0 0 2 0 0 0 0 1 1 6 1 2 1 0 0 0 0 0 1
Golgi 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 2 0 0 0 0 0 0 1
Mitochondria 161 68 119 76 142 159 140 160 118 108 138 81 117 138 44 28 24 0 0 0 0 2 5
Nucleus 53 26 12 7 6 16 24 10 24 10 15 27 28 41 21 32 30 58 7 16 6 11 18
Nuclear Periphery 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 1 0 0 1 0
Nucleolus 378 171 201 246 314 422 430 372 348 297 340 189 237 356 338 411 412 325 44 161 72 228 236
Peroxisomes 0 0 2 5 1 8 5 5 6 0 2 3 2 5 2 4 2 0 0 0 0 0 0
SpindlePole 1 0 2 5 14 23 15 11 22 12 23 2 5 10 2 2 2 9 0 3 0 3 7
Vac/Vac Membrane 2 1 0 2 0 0 1 1 1 1 1 0 2 1 3 3 4 7 0 4 0 4 4
Unique Cell Count 399 189 206 260 334 457 473 395 376 317 374 216 285 443 371 443 452 407 56 189 83 262 291
Labelled Cell Count 600 276 341 348 485 641 631 574 538 437 545 315 419 612 427 496 502 407 56 189 83 262 291


Nucleolus

(Huh et al. Nature 2003 425:686)
Screen WT1 WT2 WT3 RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3 AF100 AF140 AF180
Mean Cell GFP Intensity (1e-4) 29.3 22.5 32.9 30.1 23.2 22.9 23.4 23.2 21.7 20.7 22.1 40.2 37.5 37.2 50.3 49.0 46.8 34.5 43.3 43.8
Std Deviation (1e-4) 8.7 9.8 14.2 14.2 11.5 11.5 11.9 11.7 11.7 9.8 12.8 20.4 21.2 22.2 21.8 22.8 23.7 16.4 20.9 21.7
Intensity Change (Log2) 0.55 0.42 0.05 0.03 0.06 0.05 -0.05 -0.12 -0.03 0.84 0.74 0.73 1.16 1.13 1.06 0.62 0.95 0.96


Localization RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3
Cortical Patches 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Bud 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Bud Neck 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Bud Site 0 0 0 0 0 0 0 0 1.8 0 0 0 0 0 0
Cell Periphery 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Cytoplasm 0 0 0 0 0 0 0 0 0 2.7 4.0 4.6 0 0 2.4
Endoplasmic Reticulum 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Endosome 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Golgi 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Mitochondria 4.3 -1.5 1.5 -0.3 -1.6 1.0 -1.1 -0.4 0.2 0.3 1.1 -1.2 -6.7 -9.5 -10.1
Nucleus -2.7 -4.4 -5.5 -4.8 -3.8 -5.3 -2.9 -4.5 -4.2 -0.4 -1.3 -1.7 -3.3 -2.6 -2.9
Nuclear Periphery 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Nucleolus 3.0 1.7 1.5 0.8 0.2 1.6 0.9 1.3 0.2 -1.0 -2.3 -3.1 0.2 1.0 0.3
Peroxisomes 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
SpindlePole 0 0 2.9 3.1 0 0 3.4 0 3.5 0 0 0 0 0 0
Vacuole 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0


Endocytosis
Temp Actin Patch (Sac6-tdTomato) Cortical Patch (Sla1-GFP) Late Endosome (Snf7-GFP) Vacuole (Vph1-GFP)
37℃
RT
Cell Cycle Omics

CYCLoPs (Nop10-GFP)
Gene / Allele Actin Patch (Sac6-tdTomato) Cortical Patch (Sla1-GFP) Late Endosome (Snf7-GFP) Vacuole (Sac6-tdTomato)
Gene Images
Gene Images
Images are not yet available
Images are not yet available