Standard name
Description Protein of the AP180 family, involved in clathrin cage assembly; binds Pan1p and clathrin; YAP1801 has a paralog, YAP1802, that arose from the whole genome duplication

Micrographs

ensLOC DeepLoc
Localization WT1 WT2 WT3 RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3 WT1 WT2 WT3 AF100 AF140 AF180
Cortical Patches 0.75 0.69 0.59 0.46 0.48 0.58 0.44 0.28 0.41 0.38 0.42 0.51 0.48 0.36 0.45 0.46 0.51 0.57 0.65 0.52 0.21 0.24 0.36
Bud 0.18 0.21 0.17 0.17 0.16 0.2 0.14 0.11 0.18 0.14 0.13 0.15 0.08 0.1 0.14 0.09 0.15 0.08 0.06 0.1 0.24 0.25 0.18
Bud Neck 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0.16 0.13 0.18 0.38 0.29 0.19
Bud Site 0.28 0.13 0.07 0.1 0.07 0.11 0.1 0.05 0 0 0.07 0.13 0 0 0.05 0.09 0.06
Cell Periphery 0.12 0.18 0.14 0.2 0.08 0.09 0.15 0.11 0.21 0.09 0.17 0.25 0.33 0.5 0.21 0.2 0.22 0.08 0.08 0.08 0 0 0.05
Cytoplasm 0.08 0.18 0.22 0.3 0.31 0.18 0.27 0.24 0.19 0.18 0.17 0.29 0.3 0.37 0.27 0.22 0.16 0 0 0 0.07 0.09 0.09
Endoplasmic Reticulum 0.07 0.1 0.06 0 0.09 0.19 0.11 0.15 0.14 0.17 0.15 0.05 0.12 0.1 0 0.06 0.06 0 0 0 0 0 0
Endosome 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Golgi 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Mitochondria 0.15 0 0 0 0 0.08 0.06 0.37 0.2 0.57 0.35 0 0 0 0 0 0 0.05 0 0 0 0 0
Nucleus 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Nuclear Periphery 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Nucleolus 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Peroxisomes 0.1 0 0 0 0 0 0.05 0 0.05 0 0.05 0 0 0 0 0.06 0 0 0 0 0 0 0
SpindlePole 0 0 0 0.07 0.07 0 0.07 0 0 0 0 0 0.06 0 0.11 0.13 0.1 0 0 0 0 0 0
Vac/Vac Membrane 0 0 0 0 0 0 0 0 0.05 0 0 0 0 0 0 0.05 0 0 0 0 0 0 0
ensLOC DeepLoc
Localization WT1 WT2 WT3 RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3 WT1 WT2 WT3 AF100 AF140 AF180
Cortical Patches 94 162 82 62 93 131 132 54 100 80 95 60 117 91 123 114 97 98 174 97 32 73 100
Bud 22 48 23 23 30 45 42 21 44 30 30 18 20 26 38 22 28 12 17 17 35 76 49
Bud Neck 0 0 2 0 0 0 0 0 1 0 0 0 0 0 0 3 1 27 34 33 57 87 54
Bud Site 35 31 10 14 14 24 30 9 11 9 16 15 3 1 13 22 11
Cell Periphery 15 41 20 27 16 20 44 20 51 18 39 29 81 128 58 50 42 12 21 14 2 4 12
Cytoplasm 10 43 31 40 59 40 79 45 46 38 38 34 74 94 75 54 31 0 1 2 10 28 26
Endoplasmic Reticulum 9 23 8 6 17 43 33 29 35 35 35 6 28 26 9 16 11 3 6 3 0 2 5
Endosome 2 2 1 0 0 1 4 7 4 2 2 0 1 0 4 4 4 2 3 5 3 2 6
Golgi 1 1 0 0 0 0 4 0 0 0 0 0 0 1 11 8 5 2 1 0 0 0 0
Mitochondria 19 3 0 1 0 19 19 70 50 120 79 0 1 0 9 4 8 8 3 5 3 8 4
Nucleus 1 5 2 5 7 5 9 1 7 0 3 5 2 2 9 4 2 0 1 0 0 2 0
Nuclear Periphery 0 0 0 0 0 0 0 1 4 0 2 0 0 0 0 0 0 0 0 0 0 1 2
Nucleolus 0 0 0 0 1 0 1 2 4 1 0 0 0 0 3 2 1 0 0 0 0 0 0
Peroxisomes 12 9 0 3 6 9 16 6 13 5 12 1 3 0 5 14 3 1 0 1 0 0 0
SpindlePole 2 2 5 9 14 2 22 1 7 2 2 5 14 3 29 31 19 0 0 0 0 2 0
Vac/Vac Membrane 0 1 3 0 8 4 12 5 13 2 5 0 1 0 9 13 7 1 3 2 1 5 6
Unique Cell Count 125 234 138 134 193 226 297 190 246 211 228 118 243 256 273 247 189 172 269 188 151 302 279
Labelled Cell Count 222 371 187 190 265 343 447 271 390 342 358 173 345 372 395 361 270 172 269 188 151 302 279


Ambiguous, Bud, Bud Neck, Cell Periphery

(Huh et al. Nature 2003 425:686)
Screen WT1 WT2 WT3 RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3 AF100 AF140 AF180
Mean Cell GFP Intensity (1e-4) 7.3 8.9 8.8 8.4 9.5 7.8 8.0 6.6 7.6 6.5 7.2 8.3 8.6 8.8 11.5 11.7 11.4 7.3 7.4 7.5
Std Deviation (1e-4) 1.1 1.4 1.5 1.8 2.1 1.8 1.7 1.3 1.7 1.5 1.7 1.1 1.2 1.4 2.0 2.0 2.1 1.0 1.3 1.0
Intensity Change (Log2) -0.07 0.1 -0.18 -0.14 -0.42 -0.22 -0.45 -0.3 -0.08 -0.03 -0.01 0.38 0.41 0.37 -0.28 -0.25 -0.23


Localization RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3
Cortical Patches -2.2 -2.0 -0.3 -2.9 -5.6 -3.5 -3.9 -3.3 -1.4 -2.1 -4.6 -2.8 -2.5 -1.5
Bud 0.1 -0.3 0.8 -0.7 -1.5 0.3 -0.6 -0.9 -0.3 -2.5 -1.9 -0.7 -2.3 -0.5
Bud Neck 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Bud Site 0.9 0 1.1 1.0 -1.0 -1.1 -1.2 -0.1 1.5 0 0 -1.0 0.6 -0.5
Cell Periphery 1.2 -1.8 -1.7 0.1 -1.1 1.5 -1.7 0.7 2.0 4.0 6.9 1.6 1.4 1.8
Cytoplasm 1.4 1.6 -1.1 0.9 0.3 -0.9 -1.0 -1.4 1.2 1.7 2.9 1.1 -0.1 -1.4
Endoplasmic Reticulum -0.5 1.0 3.5 1.8 2.7 2.5 3.0 2.8 -0.2 1.8 1.5 -1.2 0.3 0
Endosome 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Golgi 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Mitochondria 0 0 3.5 3.0 8.0 5.7 10.9 7.8 0 0 0 0 0 0
Nucleus 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Nuclear Periphery 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Nucleolus 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Peroxisomes 0 0 0 2.8 0 0 0 0 0 0 0 0 2.8 0
SpindlePole 1.2 1.4 0 1.5 0 0 0 0 0 0.9 0 2.4 2.9 2.2
Vacuole 0 0 0 0 0 1.5 0 0 0 0 0 0 1.5 0


Endocytosis
Temp Actin Patch (Sac6-tdTomato) Cortical Patch (Sla1-GFP) Late Endosome (Snf7-GFP) Vacuole (Vph1-GFP)
37℃
RT
Cell Cycle Omics

CYCLoPs (Yap1801-GFP)
Gene / Allele Actin Patch (Sac6-tdTomato) Cortical Patch (Sla1-GFP) Late Endosome (Snf7-GFP) Vacuole (Sac6-tdTomato)
Gene Images
Gene Images
Images are not yet available
Images are not yet available