Standard name
Human Ortholog
Description Subunit of the THO and TREX complexes; THO connects transcription elongation and mitotic recombination, and TREX is recruited to activated genes and couples transcription to mRNA export; involved in telomere maintenance
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Micrographs

ensLOC DeepLoc
Localization WT1 WT2 WT3 RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3 WT1 WT2 WT3 AF100 AF140 AF180
Cortical Patches 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Bud 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Bud Neck 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Bud Site 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 – – – – – –
Cell Periphery 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Cytoplasm 0 0 0 0 0 0.05 0.06 0.08 0 0 0 0 0.05 0.09 0.16 0 0.05 0 0 0 0 0 0 0
Endoplasmic Reticulum 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Endosome 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Golgi 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Mitochondria 0.05 0.4 0 0 0 0.06 0.06 0 0.25 0.22 0.19 0.19 0.12 0 0 0.08 0.06 0.07 0 0 0 0 0 0
Nucleus 0.94 0.87 0.93 0.93 0.93 0.86 0.88 0.89 0.75 0.78 0.77 0.79 0.86 0.86 0.79 0.88 0.87 0.84 0.87 0.85 0.83 0.8 0.85 0.76
Nuclear Periphery 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Nucleolus 0.25 0.16 0.1 0.08 0 0.07 0.08 0.06 0.27 0.16 0.28 0.23 0.1 0.06 0.07 0.09 0.12 0.15 0 0 0.11 0.1 0.08 0.11
Peroxisomes 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
SpindlePole 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Vac/Vac Membrane 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0.06 0 0 0 0.05
ensLOC DeepLoc
Localization WT1 WT2 WT3 RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3 WT1 WT2 WT3 AF100 AF140 AF180
Cortical Patches 0 0 0 0 0 0 0 0 0 0 2 0 0 0 0 0 0 0 0 0 0 0 0 1
Bud 0 1 0 0 0 1 0 2 0 1 2 3 0 0 0 0 0 0 0 1 0 0 1 4
Bud Neck 0 0 1 0 0 0 0 0 0 0 1 0 0 0 0 0 0 0 0 0 0 0 0 0
Bud Site 0 0 0 1 1 7 4 3 5 8 5 8 0 0 0 0 0 0 – – – – – –
Cell Periphery 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Cytoplasm 1 0 2 5 11 13 18 23 6 8 5 7 6 21 52 6 10 5 1 0 1 1 2 0
Endoplasmic Reticulum 1 0 1 0 0 1 0 0 0 0 0 0 0 0 0 5 1 6 0 0 1 0 0 0
Endosome 0 0 0 0 0 1 0 1 0 0 0 0 2 0 1 2 0 3 0 1 0 0 2 1
Golgi 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 1 0 0 0 1 1
Mitochondria 4 56 0 1 1 15 19 11 37 49 45 45 15 5 9 13 13 12 0 2 0 1 0 1
Nucleus 78 123 127 133 228 211 268 267 110 177 185 186 111 196 256 153 187 151 82 128 121 94 218 252
Nuclear Periphery 0 0 0 2 2 3 3 1 2 0 1 2 0 3 2 1 5 0 0 0 0 0 0 0
Nucleolus 21 22 14 12 8 17 24 18 40 37 66 54 13 14 22 15 25 27 3 5 15 11 20 36
Peroxisomes 0 0 0 0 0 0 0 0 0 0 0 0 0 1 0 0 0 1 0 0 0 0 0 1
SpindlePole 0 1 0 0 3 2 2 4 1 1 0 1 1 3 6 1 2 5 0 0 0 0 1 8
Vac/Vac Membrane 0 1 3 3 4 9 0 1 5 6 3 1 1 4 5 0 4 4 1 8 3 5 7 16
Unique Cell Count 83 141 137 143 245 246 303 301 147 226 240 236 129 227 323 173 215 180 94 151 147 118 259 331
Labelled Cell Count 105 204 148 157 258 280 338 331 206 287 315 307 149 247 353 196 247 214 94 151 147 118 259 331


Cytoplasm, Nucleus

(Huh et al. Nature 2003 425:686)
Screen WT1 WT2 WT3 RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3 AF100 AF140 AF180
Mean Cell GFP Intensity (1e-4) 6.6 4.5 6.9 7.0 7.5 6.3 5.9 6.8 5.4 5.6 5.4 5.8 5.9 7.0 6.5 6.6 8.1 7.9 8.1 8.3 7.9
Std Deviation (1e-4) 1.2 0.8 1.5 1.9 2.0 2.2 1.6 1.9 1.7 1.7 1.6 1.7 1.6 2.0 2.0 1.8 2.8 2.7 2.0 2.2 1.7
Intensity Change (Log2) – – – 0.02 0.11 -0.13 -0.23 -0.04 -0.36 -0.3 -0.35 -0.25 -0.22 0.02 -0.1 -0.07 0.23 0.19 0.22 0.26 0.2


Localization RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3
Actin – – – – – – – – – – – – – – –
Bud – – – – – – – – – – – – – – –
Bud Neck – – – – – – – – – – – – – – –
Bud Site – – – – – – – – – – – – – – –
Cell Periphery – – – – – – – – – – – – – – –
Cyto – – – – – – – – – – – – – – –
Endoplasmic Reticulum – – – – – – – – – – – – – – –
Endosome – – – – – – – – – – – – – – –
Golgi – – – – – – – – – – – – – – –
Mitochondria – – – – – – – – – – – – – – –
Nuclear Periphery – – – – – – – – – – – – – – –
Nuc – – – – – – – – – – – – – – –
Nucleolus – – – – – – – – – – – – – – –
Peroxisomes – – – – – – – – – – – – – – –
SpindlePole – – – – – – – – – – – – – – –
Vac – – – – – – – – – – – – – – –
Cortical Patches – – – – – – – – – – – – – – –
Cytoplasm – – – – – – – – – – – – – – –
Nucleus – – – – – – – – – – – – – – –
Vacuole – – – – – – – – – – – – – – –

Images

Circle plot not available

Protein Concentration and Protein Localization Data

R1 R2 R3
G1 Pre-START G1 Post-START S/G2 Metaphase Anaphase Telophase G1 Pre-START G1 Post-START S/G2 Metaphase Anaphase Telophase G1 Pre-START G1 Post-START S/G2 Metaphase Anaphase Telophase
Concentration 2.2839 2.4909 2.47 1.7555 1.9872 2.3553 5.5422 7.0342 5.8995 6.8922 5.9858 5.9428 2.4068 3.4292 2.7359 2.9209 2.3638 2.5031
Actin 0.0149 0 0.0086 0.0001 0.0123 0.0025 0.0144 0 0.0033 0 0.0001 0.0004 0.0082 0 0.0049 0 0.0123 0.0009
Bud 0.0002 0 0.0001 0.0001 0.0003 0.0001 0.0003 0 0.0001 0 0.0003 0 0.0003 0 0.0002 0 0.0001 0.0001
Bud Neck 0.001 0.0001 0.0002 0.0002 0.0009 0.0008 0.0011 0.0001 0.0009 0.0001 0.0009 0.0006 0.0002 0.0001 0.0003 0.0001 0.0004 0.0007
Bud Periphery 0.0006 0 0.0001 0.0001 0.0011 0.0004 0.001 0 0.0003 0 0.0013 0.0001 0.0007 0 0.0004 0 0.0002 0.0001
Bud Site 0.0006 0 0.0002 0.0002 0.0011 0.0001 0.0013 0 0.0044 0 0.0005 0.0002 0.0005 0 0.0025 0 0.0004 0.0008
Cell Periphery 0.0003 0 0 0.0001 0.0005 0.0001 0.0003 0 0.0002 0 0.0008 0 0.0002 0 0.0002 0 0.0001 0
Cytoplasm 0.0004 0 0.0006 0.0006 0.0012 0.0002 0.0024 0 0.0005 0.0001 0.0009 0.0001 0.0056 0 0.0088 0.0001 0.0001 0
Cytoplasmic Foci 0.0146 0 0.0018 0.0032 0.0141 0.001 0.0112 0 0.0027 0 0.0001 0.004 0.0004 0 0.0052 0 0.0011 0.0017
Eisosomes 0.0003 0 0.0001 0 0.0003 0 0.0002 0 0.0001 0 0 0 0.0001 0 0.0001 0 0.0002 0
Endoplasmic Reticulum 0.0013 0 0.0005 0.0003 0.0006 0.0011 0.0023 0 0.0003 0 0.0007 0 0.0012 0 0.0012 0 0.0002 0
Endosome 0.0091 0 0.0074 0.002 0.0044 0.0071 0.0139 0 0.0025 0 0.0006 0.001 0.0035 0 0.0049 0 0.0037 0.001
Golgi 0.0045 0 0.0014 0.0003 0.003 0.0051 0.0039 0 0.0009 0 0 0.0012 0.002 0 0.0019 0 0.0106 0.0012
Lipid Particles 0.0123 0 0.0028 0.0082 0.0191 0.0007 0.0135 0 0.0021 0 0.0002 0.0012 0.0017 0 0.0068 0 0.0037 0.0016
Mitochondria 0.005 0.0001 0.0009 0.0005 0.0301 0.0029 0.0028 0.0001 0.0094 0.0001 0.001 0.0005 0.0087 0.0001 0.0054 0.0001 0.01 0.0025
None 0.0004 0 0.0012 0.0005 0.0019 0.0001 0.002 0 0.0002 0.0001 0.0005 0 0.0097 0 0.0025 0 0.0001 0
Nuclear Periphery 0.0088 0.0013 0.0031 0.0046 0.0132 0.0047 0.0156 0.0003 0.0017 0.0007 0.0047 0.0001 0.0084 0.0002 0.0027 0.0006 0.0031 0.0001
Nucleolus 0.0442 0.0306 0.0229 0.0424 0.1114 0.0847 0.0426 0.0331 0.0309 0.0141 0.1838 0.0855 0.039 0.0277 0.0235 0.0097 0.1362 0.0822
Nucleus 0.8637 0.9654 0.9372 0.9218 0.7448 0.8869 0.8507 0.9657 0.9267 0.9811 0.7852 0.9027 0.9067 0.9714 0.9097 0.9504 0.8137 0.9038
Peroxisomes 0.0088 0 0.0007 0.0003 0.0169 0.0001 0.0061 0 0.0059 0 0.0001 0.0006 0.0005 0 0.0107 0 0.0008 0.0028
Punctate Nuclear 0.0067 0.0026 0.0089 0.0131 0.0192 0.0004 0.011 0.0006 0.0056 0.0038 0.0109 0.0017 0.0009 0.0003 0.0067 0.0388 0.0027 0.0003
Vacuole 0.0012 0 0.0007 0.001 0.0016 0.0004 0.0023 0 0.0008 0 0.0061 0.0001 0.0006 0 0.0011 0 0.0002 0.0001
Vacuole Periphery 0.0009 0 0.0006 0.0007 0.0022 0.0006 0.0012 0 0.0004 0 0.0013 0 0.0011 0 0.0003 0 0.0002 0

Sequencing Data

R1 R2
G1 Post-START S/G2 Metaphase Anaphase Telophase G1 Post-START S/G2 Metaphase Anaphase Telophase
Gene Expression 20.3554 12.5493 8.3809 12.2968 9.1006 8.4183 12.3714 16.6997 14.5285 14.6283
Translational Efficiency 1.0293 1.8068 1.8241 1.184 1.9491 1.9951 1.9442 1.1265 1.1479 1.4669

Hit Data

Dataset Hit
Protein Abundance ✘
Protein Localization ✘
Gene Expression ✘
Translational Efficiency ✘