Standard name
Description Enolase II, a phosphopyruvate hydratase; catalyzes conversion of 2-phosphoglycerate to phosphoenolpyruvate during glycolysis and the reverse reaction during gluconeogenesis; expression induced in response to glucose; ENO2 has a paralog, ENO1, that arose from the whole genome duplication
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Micrographs

ensLOC DeepLoc
Localization WT1 WT2 WT3 RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3 WT1 WT2 WT3 AF100 AF140 AF180
Cortical Patches – – 0 0 0 0 0 0 0 0 0 0 – 0 0 0 0 0 0 0 0 0 0 0
Bud – – 0 0 0 0 0 0 0 0 0 0 – 0 0 0 0 0 0 0 0 0 0 0
Bud Neck – – 0 0 0 0 0 0 0 0 0 0 – 0 0 0 0 0 0 0 0 0 0 0
Bud Site – – 0 0 0 0 0 0 0 0 0 0 – 0 0 0 0 0 – – – – – –
Cell Periphery – – 0.73 0.82 0.78 0.74 0.73 0.76 0.68 0.71 0.59 0.62 – 0.67 0.74 0.62 0.75 0.67 0.79 0.91 0.76 0.69 0.84 0.85
Cytoplasm – – 0.33 0.23 0.31 0.34 0.33 0.3 0.4 0.39 0.53 0.47 – 0.38 0.27 0.38 0.24 0.29 0.17 0.08 0.21 0.29 0.14 0.13
Endoplasmic Reticulum – – 0 0 0 0 0 0 0 0 0 0 – 0 0 0 0.06 0.1 0 0 0 0 0 0
Endosome – – 0 0 0 0 0 0 0 0 0 0 – 0 0 0 0 0 0 0 0 0 0 0
Golgi – – 0 0 0 0 0 0 0 0 0 0 – 0 0 0 0 0 0 0 0 0 0 0
Mitochondria – – 0 0 0 0 0 0 0 0 0 0 – 0 0 0 0 0 0 0 0 0 0 0
Nucleus – – 0 0 0 0 0 0 0 0 0 0 – 0 0 0 0 0 0 0 0 0 0 0
Nuclear Periphery – – 0 0 0 0 0 0 0 0 0 0 – 0 0 0 0 0 0 0 0 0 0 0
Nucleolus – – 0 0 0 0 0 0 0 0 0 0 – 0 0 0 0 0 0 0 0 0 0 0
Peroxisomes – – 0 0 0 0 0 0 0 0 0 0 – 0 0 0 0 0 0 0 0 0 0 0
SpindlePole – – 0 0 0 0 0 0 0 0 0 0 – 0 0 0 0 0 0 0 0 0 0 0
Vac/Vac Membrane – – 0 0 0 0 0 0 0 0 0 0 – 0 0 0 0 0.05 0 0 0 0 0 0
ensLOC DeepLoc
Localization WT1 WT2 WT3 RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3 WT1 WT2 WT3 AF100 AF140 AF180
Cortical Patches – – 0 0 0 0 0 0 0 0 0 0 – 0 0 0 0 0 0 0 0 0 0 0
Bud – – 0 0 0 1 0 0 0 1 1 0 – 0 0 0 0 0 0 0 0 0 0 0
Bud Neck – – 0 0 0 0 0 0 0 0 0 0 – 0 0 0 0 0 0 0 1 0 0 0
Bud Site – – 0 0 0 0 0 0 0 0 0 0 – 0 0 0 0 0 – – – – – –
Cell Periphery – – 62 78 70 135 159 172 113 194 108 158 – 26 58 10 47 14 26 50 187 35 137 154
Cytoplasm – – 28 22 28 61 71 68 67 108 98 121 – 15 21 6 15 6 5 4 53 14 23 23
Endoplasmic Reticulum – – 0 0 0 0 0 0 0 0 0 0 – 0 0 0 4 2 0 0 1 0 0 0
Endosome – – 0 0 0 0 0 0 0 0 0 0 – 0 0 0 0 0 0 0 0 0 0 0
Golgi – – 0 0 0 0 0 0 0 0 0 0 – 0 0 0 0 0 0 0 0 0 0 0
Mitochondria – – 0 0 0 0 0 0 0 0 0 0 – 0 0 0 0 0 0 0 0 0 0 0
Nucleus – – 0 0 0 0 0 1 0 0 0 0 – 0 0 0 0 0 0 0 1 0 1 0
Nuclear Periphery – – 0 0 0 0 0 0 0 0 0 0 – 0 0 0 0 0 0 0 0 0 0 0
Nucleolus – – 0 0 0 0 0 0 0 0 0 0 – 0 0 0 0 0 0 0 0 0 0 0
Peroxisomes – – 0 0 0 0 0 0 0 0 0 0 – 0 0 0 0 0 0 0 0 0 0 0
SpindlePole – – 0 0 0 0 0 0 0 0 0 0 – 0 0 0 0 0 0 0 0 0 0 0
Vac/Vac Membrane – – 0 0 0 0 0 0 0 0 0 0 – 0 0 0 0 1 0 0 0 0 0 0
Unique Cell Count 85 95 90 182 218 227 167 274 184 255 39 78 16 63 21 33 56 248 51 163 182
Labelled Cell Count 90 100 98 197 230 241 180 303 207 279 41 79 16 66 23 33 56 248 51 163 182


Cytoplasm

(Huh et al. Nature 2003 425:686)
Screen WT1 WT2 WT3 RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3 AF100 AF140 AF180
Mean Cell GFP Intensity (1e-4) – – 750.9 748.9 732.8 692.7 677.1 650.2 630.7 627.6 588.0 595.4 – 799.5 775.1 835.2 799.6 776.3 – – 778.8
Std Deviation (1e-4) – – 132.6 126.7 128.4 107.0 113.6 111.9 100.6 92.8 91.5 94.4 – 163.9 138.4 151.7 144.7 167.2 – – 214.7
Intensity Change (Log2) – – – -0.0 -0.04 -0.12 -0.15 -0.21 -0.25 -0.26 -0.35 -0.33 – 0.09 0.05 0.15 0.09 0.05 – – 0.05


Localization RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3
Cortical Patches 0 0 0 0 0 0 0 0 0 – 0 0 0 0 0
Bud 0 0 0 0 0 0 0 0 0 – 0 0 0 0 0
Bud Neck 0 0 0 0 0 0 0 0 0 – 0 0 0 0 0
Bud Site 0 0 0 0 0 0 0 0 0 – 0 0 0 0 0
Cell Periphery 1.5 0.7 0.2 0 0.5 -0.9 -0.4 -2.3 -1.8 – -0.7 0.2 -0.8 0.2 -0.6
Cytoplasm -1.5 -0.3 0.1 -0.1 -0.5 1.1 1.1 3.1 2.3 – 0.6 -0.8 0.4 -1.2 -0.4
Endoplasmic Reticulum 0 0 0 0 0 0 0 0 0 – 0 0 0 0 0
Endosome 0 0 0 0 0 0 0 0 0 – 0 0 0 0 0
Golgi 0 0 0 0 0 0 0 0 0 – 0 0 0 0 0
Mitochondria 0 0 0 0 0 0 0 0 0 – 0 0 0 0 0
Nucleus 0 0 0 0 0 0 0 0 0 – 0 0 0 0 0
Nuclear Periphery 0 0 0 0 0 0 0 0 0 – 0 0 0 0 0
Nucleolus 0 0 0 0 0 0 0 0 0 – 0 0 0 0 0
Peroxisomes 0 0 0 0 0 0 0 0 0 – 0 0 0 0 0
SpindlePole 0 0 0 0 0 0 0 0 0 – 0 0 0 0 0
Vacuole 0 0 0 0 0 0 0 0 0 – 0 0 0 0 0