Standard name
Description One of three repressible acid phosphatases; glycoprotein that is transported to the cell surface by the secretory pathway; pregulated by phosphate starvation; PHO12 has a paralog, PHO11, that arose from a segmental duplication

Micrographs

ensLOC DeepLoc
Localization WT1 WT2 WT3 RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3 WT1 WT2 WT3 AF100 AF140 AF180
Cortical Patches 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0.06 0 0 0
Bud 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Bud Neck 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Bud Site 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Cell Periphery 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Cytoplasm 0.7 0.81 0.98 0.95 0.86 0.86 0.86 0.92 0.89 0.89 0.75 0.72 0.95 0.99 0.99 0.76 0.67 0.49 0.6 0.5 0.75 0.74 0.75
Endoplasmic Reticulum 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0.06 0 0.06 0 0 0 0 0
Endosome 0.06 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Golgi 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Mitochondria 0.19 0.11 0 0.09 0.13 0.2 0.26 0.2 0.25 0.2 0.46 0.4 0 0 0 0 0 0.14 0.05 0.25 0.05 0 0.05
Nucleus 0 0 0 0.07 0 0 0 0 0 0 0 0 0 0 0 0.05 0 0 0 0 0 0 0
Nuclear Periphery 0 0 0 0 0 0 0 0 0 0 0 0.05 0 0 0 0 0 0 0 0 0 0 0
Nucleolus 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Peroxisomes 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
SpindlePole 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Vac/Vac Membrane 0.13 0.12 0 0 0.06 0 0 0 0 0 0 0 0 0 0 0.22 0.32 0.2 0.21 0.08 0.09 0.09 0.08
ensLOC DeepLoc
Localization WT1 WT2 WT3 RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3 WT1 WT2 WT3 AF100 AF140 AF180
Cortical Patches 0 0 0 0 0 0 0 0 0 0 0 0 1 0 1 0 0 1 2 3 2 1 2
Bud 0 0 1 0 0 2 0 2 1 0 1 3 1 0 1 0 0 1 1 0 4 5 4
Bud Neck 1 0 1 1 3 2 1 2 0 0 1 1 0 0 0 1 0 1 1 0 0 0 3
Bud Site 0 0 0 0 0 0 0 1 0 0 1 0 0 0 0 0 0
Cell Periphery 0 1 1 0 0 0 1 0 2 2 0 0 1 3 2 0 0 0 0 1 1 0 1
Cytoplasm 48 108 45 42 72 123 127 193 103 110 98 62 292 274 300 118 55 65 93 31 110 160 208
Endoplasmic Reticulum 0 2 0 0 0 0 0 0 0 0 1 1 5 0 1 10 2 8 0 0 0 2 2
Endosome 4 1 0 0 0 0 0 0 0 0 0 0 1 1 0 5 2 5 5 0 1 1 7
Golgi 0 0 0 0 0 0 0 0 0 0 0 0 1 0 0 2 1 0 1 0 0 1 1
Mitochondria 13 15 2 4 11 28 38 42 29 25 60 34 1 2 1 4 1 18 7 15 7 8 14
Nucleus 2 0 0 3 1 4 2 3 1 2 1 1 1 0 2 8 3 0 1 0 0 0 0
Nuclear Periphery 0 0 0 0 0 0 0 0 0 0 1 4 0 0 0 0 0 0 1 0 0 0 0
Nucleolus 1 0 0 0 0 0 0 0 0 0 0 1 0 0 0 0 0 0 0 0 0 3 0
Peroxisomes 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 1
SpindlePole 1 0 0 1 0 1 1 2 1 0 1 2 1 0 0 3 3 0 1 0 1 2 3
Vac/Vac Membrane 9 16 1 0 5 5 4 2 3 0 1 0 7 0 0 34 26 26 31 5 13 19 21
Unique Cell Count 69 133 46 44 84 143 147 209 116 123 130 86 307 276 304 156 82 133 154 62 147 217 280
Labelled Cell Count 79 143 51 51 92 165 174 247 140 139 166 109 312 280 308 185 93 133 154 62 147 217 280


Vacuole

(Huh et al. Nature 2003 425:686)
Screen WT1 WT2 WT3 RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3 AF100 AF140 AF180
Mean Cell GFP Intensity (1e-4) 3.9 4.0 3.1 3.0 3.6 3.2 2.7 3.1 3.6 2.9 3.1 3.0 4.4 4.4 4.5 4.8 6.1 6.0 4.2 4.1 4.9
Std Deviation (1e-4) 1.2 1.2 0.7 1.3 1.5 1.7 1.3 1.7 2.1 1.8 2.1 2.1 1.4 1.8 2.1 1.7 2.1 1.8 2.4 1.8 2.6
Intensity Change (Log2) -0.02 0.23 0.06 -0.18 0.02 0.22 -0.09 0.02 -0.03 0.52 0.52 0.54 0.64 0.99 0.98 0.47 0.41 0.68


Localization RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3
Actin
Bud 0 0
Bud Neck 0 0
Bud Site 0 0
Cell Periphery 0 0
Cyto
Endoplasmic Reticulum 0 0
Endosome 0 0
Golgi 0 0
Mitochondria 0 0
Nuclear Periphery 0 0
Nuc
Nucleolus 0 0
Peroxisomes 0 0
SpindlePole 0 0
Vac
Cortical Patches 0 0
Cytoplasm 0 0
Nucleus 0 0
Vacuole 3.1 3.9


Endocytosis
Temp Actin Patch (Sac6-tdTomato) Cortical Patch (Sla1-GFP) Late Endosome (Snf7-GFP) Vacuole (Vph1-GFP)
37℃
RT
Cell Cycle Omics

CYCLoPs (Pho12-GFP)
Gene / Allele Actin Patch (Sac6-tdTomato) Cortical Patch (Sla1-GFP) Late Endosome (Snf7-GFP) Vacuole (Sac6-tdTomato)
Gene Images
Gene Images
Images are not yet available
Images are not yet available