Standard name
Human Ortholog
Description ATPase component of the RSC chromatin remodeling complex; required for expression of early meiotic genes; promotes base excision repair in chromatin; essential helicase-related protein homologous to Snf2p

Micrographs

ensLOC DeepLoc
Localization WT1 WT2 WT3 RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3 WT1 WT2 WT3 AF100 AF140 AF180
Cortical Patches 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Bud 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Bud Neck 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Bud Site 0 0 0 0 0 0 0 0 0 0 0.05 0 0 0
Cell Periphery 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Cytoplasm 0 0 0 0 0.06 0 0.05 0 0.05 0 0.07 0.09 0.07 0.07 0 0 0 0 0 0
Endoplasmic Reticulum 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Endosome 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Golgi 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Mitochondria 0 0 0.05 0.05 0 0.08 0.06 0.12 0.13 0.1 0.11 0 0 0 0 0 0 0 0 0
Nucleus 0.84 0.84 0.82 0.83 0.77 0.74 0.69 0.64 0.62 0.62 0.6 0.79 0.76 0.72 0.69 0.81 0.73 0.68 0.51 0.55
Nuclear Periphery 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Nucleolus 0.35 0.33 0.39 0.33 0.32 0.43 0.44 0.47 0.52 0.62 0.6 0.37 0.32 0.39 0.26 0.12 0.21 0.26 0.4 0.35
Peroxisomes 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
SpindlePole 0 0 0 0 0 0 0 0 0 0 0 0 0 0.05 0 0 0 0 0 0
Vac/Vac Membrane 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0.05
ensLOC DeepLoc
Localization WT1 WT2 WT3 RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3 WT1 WT2 WT3 AF100 AF140 AF180
Cortical Patches 0 0 0 0 0 0 0 0 0 0 0 0 0 1 0 0 0 0 0 1
Bud 0 0 1 0 0 1 1 0 0 1 1 0 0 2 0 3 1 0 2 2
Bud Neck 0 0 0 0 0 1 2 0 1 0 1 0 0 0 0 0 0 0 0 0
Bud Site 0 0 1 0 4 14 15 11 11 7 13 0 1 4
Cell Periphery 0 0 0 0 0 0 0 1 0 0 0 0 1 0 0 0 0 0 0 0
Cytoplasm 4 15 8 10 16 10 19 15 15 5 18 32 35 34 1 2 0 0 0 1
Endoplasmic Reticulum 0 0 0 0 0 0 0 0 0 0 1 0 0 0 0 1 0 0 2 1
Endosome 1 3 0 0 0 0 0 0 0 0 0 0 1 1 2 4 4 0 3 2
Golgi 0 0 0 0 0 0 0 0 0 0 0 0 0 0 1 0 2 1 2 0
Mitochondria 0 4 17 14 6 26 24 49 38 21 29 3 5 6 1 1 1 0 2 4
Nucleus 274 350 285 213 198 254 280 256 188 138 160 273 373 334 249 366 271 240 185 252
Nuclear Periphery 2 0 3 0 1 4 1 4 5 2 5 1 2 1 0 0 0 1 0 0
Nucleolus 113 139 135 86 83 145 181 187 158 138 159 127 157 181 93 54 79 93 148 161
Peroxisomes 1 1 0 0 1 1 0 0 0 0 0 1 5 5 0 0 0 0 0 0
SpindlePole 1 0 1 1 1 1 2 4 1 0 0 3 17 22 3 3 0 2 2 5
Vac/Vac Membrane 0 3 2 3 2 0 1 2 1 0 1 1 4 3 4 11 8 9 8 21
Unique Cell Count 325 419 349 257 257 341 407 402 302 221 267 346 491 461 361 452 373 354 367 461
Labelled Cell Count 396 515 453 327 312 457 526 529 418 312 388 441 601 594 361 452 373 354 367 461


Nucleus

(Huh et al. Nature 2003 425:686)
Screen WT1 WT2 WT3 RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3 AF100 AF140 AF180
Mean Cell GFP Intensity (1e-4) 16.6 13.1 8.7 8.7 9.0 7.8 7.9 7.1 7.1 6.8 6.6 11.9 10.9 9.9 11.6 12.2 12.5
Std Deviation (1e-4) 4.2 3.7 2.5 3.2 2.7 2.0 2.3 2.1 2.1 1.9 1.9 3.7 3.6 3.5 2.9 3.1 2.9
Intensity Change (Log2) 0.01 0.06 -0.15 -0.14 -0.28 -0.29 -0.36 -0.4 0.46 0.32 0.18 0.41 0.49 0.53


Localization RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3
Cortical Patches 0 0 0 0 0 0 0 0 0 0 0
Bud 0 0 0 0 0 0 0 0 0 0 0
Bud Neck 0 0 0 0 0 0 0 0 0 0 0
Bud Site 0 0 3.4 3.2 2.7 3.2 0 3.8 0 0 0
Cell Periphery 0 0 0 0 0 0 0 0 0 0 0
Cytoplasm 1.1 2.5 0.5 1.8 1.1 1.8 0 2.7 3.9 3.1 3.2
Endoplasmic Reticulum 0 0 0 0 0 0 0 0 0 0 0
Endosome 0 0 0 0 0 0 0 0 0 0 0
Golgi 0 0 0 0 0 0 0 0 0 0 0
Mitochondria 0.3 -1.6 1.5 0.6 3.5 3.5 2.2 2.8 -3.2 -3.4 -3.0
Nucleus 0.4 -1.4 -2.3 -4.1 -5.5 -5.5 -5.1 -6.0 -0.9 -2.0 -3.1
Nuclear Periphery 0 0 0 0 0 0 0 0 0 0 0
Nucleolus -1.3 -1.6 1.0 1.6 2.2 3.5 5.5 5.1 -0.5 -2.0 0.2
Peroxisomes 0 0 0 0 0 0 0 0 0 0 0
SpindlePole 0 0 0 0 0 0 0 0 0 3.1 3.8
Vacuole 0 0 0 0 0 0 0 0 0 0 0

Images

Circle plot not available

Protein Concentration and Protein Localization Data

R1 R2 R3
G1 Pre-START G1 Post-START S/G2 Metaphase Anaphase Telophase G1 Pre-START G1 Post-START S/G2 Metaphase Anaphase Telophase G1 Pre-START G1 Post-START S/G2 Metaphase Anaphase Telophase
Concentration 15.8224 14.5592 14.4941 13.8944 14.136 13.8048 9.4329 7.9412 8.3391 7.801 7.0875 7.7222 15.1274 14.2401 14.3242 14.3139 13.1046 13.504
Actin 0 0 0 0 0 0 0 0 0 0 0.0012 0 0.0048 0 0.0006 0 0.0395 0
Bud 0 0 0 0 0 0 0.0001 0 0 0 0 0 0.0004 0 0 0 0.0001 0
Bud Neck 0 0 0 0 0.0002 0.0004 0.0001 0 0 0 0.0002 0.0001 0.0001 0 0.0001 0 0.0004 0.0002
Bud Periphery 0 0 0 0 0.0001 0.0001 0.0001 0 0 0 0.0001 0 0.0004 0 0 0 0.0002 0
Bud Site 0 0 0 0 0 0.0001 0.0003 0 0 0 0.0001 0 0.0007 0 0.0004 0 0.0001 0
Cell Periphery 0 0 0 0 0 0 0.0001 0 0 0 0.0001 0 0.0002 0 0 0 0.0001 0
Cytoplasm 0 0 0 0 0 0 0.0001 0 0 0 0.001 0 0.0034 0 0.0002 0 0.0003 0
Cytoplasmic Foci 0 0 0 0 0 0 0.0001 0 0 0 0.0006 0 0.0044 0 0.0069 0 0.0029 0
Eisosomes 0 0 0 0 0 0 0 0 0 0 0.0003 0 0.0002 0 0 0 0.0005 0
Endoplasmic Reticulum 0 0 0 0 0 0 0 0 0 0 0.0009 0 0.0007 0 0 0 0.0004 0
Endosome 0 0 0 0 0 0 0 0 0 0 0.0062 0 0.0029 0 0.0016 0 0.0038 0
Golgi 0 0 0 0 0 0 0 0 0 0 0.0008 0 0.0007 0 0.0024 0 0.0041 0
Lipid Particles 0 0 0 0 0 0 0.0001 0 0 0 0.0032 0 0.001 0 0.0033 0 0.013 0
Mitochondria 0.0003 0.0001 0.0001 0 0.0007 0.0001 0.0006 0.0001 0 0 0.001 0 0.0017 0 0.0006 0 0.0014 0.0001
None 0 0 0 0 0 0 0 0 0 0 0.009 0 0.0096 0 0.0003 0 0.0033 0
Nuclear Periphery 0 0.0002 0.0002 0 0.0001 0 0.0001 0.0004 0.0001 0 0.0116 0 0.0099 0.0002 0.0002 0.0001 0.0019 0
Nucleolus 0.0978 0.0491 0.0439 0.0334 0.3758 0.2246 0.087 0.043 0.031 0.0242 0.3155 0.175 0.0847 0.052 0.0437 0.0276 0.4183 0.2262
Nucleus 0.9017 0.9504 0.9557 0.9665 0.6226 0.7745 0.9106 0.9563 0.9688 0.9756 0.6411 0.8246 0.8702 0.9476 0.9286 0.9722 0.4942 0.7732
Peroxisomes 0 0 0 0 0 0 0 0 0 0 0.0002 0 0.0009 0 0.0015 0 0.0098 0
Punctate Nuclear 0 0.0001 0 0 0.0004 0.0001 0.0002 0.0002 0 0 0.0055 0.0001 0.0018 0.0001 0.0092 0 0.0051 0.0001
Vacuole 0 0 0 0 0.0001 0.0001 0.0002 0 0 0 0.0006 0 0.0007 0 0.0001 0 0.0002 0
Vacuole Periphery 0 0 0 0 0.0001 0 0.0001 0 0 0 0.0007 0 0.0008 0 0 0 0.0003 0

Sequencing Data

R1 R2
G1 Post-START S/G2 Metaphase Anaphase Telophase G1 Post-START S/G2 Metaphase Anaphase Telophase
Gene Expression 35.2927 49.077 41.6198 44.5118 42.7781 36.7598 49.4384 44.8751 43.6194 47.3326
Translational Efficiency 0.8889 0.7558 0.7679 0.6709 0.6139 0.688 0.6312 0.5434 0.5526 0.5534

Hit Data

Dataset Hit
Protein Concentration
Protein Localization
Gene Expression
Translational Efficiency