Standard name
Description Subunit of the 26S proteasome; substrate of the N-acetyltransferase Nat1p; protein abundance increases in response to DNA replication stress
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Micrographs

ensLOC DeepLoc
Localization WT1 WT2 WT3 RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3 WT1 WT2 WT3 AF100 AF140 AF180
Cortical Patches 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Bud 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Bud Neck 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Bud Site 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 – – – – – –
Cell Periphery 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Cytoplasm 0.05 0 0 0.11 0.16 0.2 0.27 0.27 0.25 0.29 0.17 0.15 0.09 0.12 0.13 0.08 0.1 0.05 0 0 0 0 0 0
Endoplasmic Reticulum 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Endosome 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Golgi 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0.09 0.22 0.15 0 0 0 0 0 0
Mitochondria 0 0 0 0 0 0 0 0 0.11 0.08 0.05 0.12 0 0 0 0 0 0 0 0 0 0 0 0
Nucleus 0.71 0.66 0.71 0.59 0.34 0.24 0.15 0.19 0.22 0.2 0.29 0.27 0.55 0.46 0.44 0.25 0.23 0.22 0.78 0.81 0.73 0.78 0.74 0.61
Nuclear Periphery 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Nucleolus 0 0.06 0 0 0 0 0 0 0 0 0.06 0.05 0.09 0 0 0 0 0 0.1 0.08 0.07 0 0.08 0.1
Peroxisomes 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
SpindlePole 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Vac/Vac Membrane 0.24 0.34 0.29 0.28 0.53 0.59 0.64 0.5 0.44 0.42 0.46 0.47 0.36 0.35 0.42 0.69 0.6 0.7 0.07 0.07 0.12 0.15 0.13 0.21
ensLOC DeepLoc
Localization WT1 WT2 WT3 RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3 WT1 WT2 WT3 AF100 AF140 AF180
Cortical Patches 0 0 0 0 0 0 0 0 1 0 1 0 0 1 1 1 0 0 0 0 0 0 0 0
Bud 0 0 0 0 0 1 1 1 1 3 1 1 0 0 0 0 0 0 1 2 1 0 3 2
Bud Neck 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 1 0 0 0 0 0
Bud Site 0 0 0 0 0 0 0 0 1 2 0 2 0 0 0 0 0 0 – – – – – –
Cell Periphery 0 0 0 0 0 0 1 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Cytoplasm 9 1 1 8 22 33 71 70 52 68 35 35 2 17 20 24 19 13 0 0 0 0 2 1
Endoplasmic Reticulum 0 0 0 2 0 0 3 2 3 6 2 1 0 0 0 6 8 7 0 0 0 0 0 1
Endosome 0 0 0 0 0 0 0 1 0 0 0 0 0 1 0 0 0 0 0 0 3 0 1 2
Golgi 4 1 0 0 2 0 3 9 3 2 2 0 0 2 2 29 43 36 0 0 0 0 0 1
Mitochondria 0 0 2 1 1 5 2 4 22 19 11 28 0 0 1 2 0 0 0 0 0 0 0 0
Nucleus 131 57 87 44 46 40 41 49 47 47 60 64 12 63 66 78 45 53 172 104 132 70 159 125
Nuclear Periphery 1 1 0 1 1 0 1 4 3 0 0 0 0 0 0 0 1 0 2 0 3 1 0 0
Nucleolus 5 5 2 1 2 1 4 8 8 10 12 12 2 5 2 5 4 3 22 9 13 3 16 21
Peroxisomes 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
SpindlePole 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Vac/Vac Membrane 45 30 36 21 72 98 171 128 91 101 95 111 8 48 63 218 116 171 15 8 22 14 28 42
Unique Cell Count 184 87 123 75 136 166 267 255 209 238 206 235 22 136 151 315 192 245 220 129 181 91 216 205
Labelled Cell Count 195 95 128 78 146 178 298 276 232 258 219 254 24 137 155 363 236 283 220 129 181 91 216 205


Cytoplasm, Nucleus

(Huh et al. Nature 2003 425:686)
Screen WT1 WT2 WT3 RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3 AF100 AF140 AF180
Mean Cell GFP Intensity (1e-4) 31.5 26.1 16.8 17.3 18.4 18.6 17.9 17.2 15.1 14.8 14.0 13.5 31.0 28.8 24.1 27.2 30.3 31.8 27.6 25.5 27.0
Std Deviation (1e-4) 7.2 5.4 3.6 4.4 4.2 4.4 3.8 4.1 3.7 4.0 3.6 3.6 8.1 7.8 6.5 6.9 7.7 8.1 7.1 7.1 8.4
Intensity Change (Log2) – – – 0.04 0.13 0.15 0.09 0.04 -0.15 -0.18 -0.26 -0.31 0.88 0.78 0.52 0.7 0.85 0.92 0.72 0.61 0.69


Localization RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3
Cortical Patches 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Bud 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Bud Neck 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Bud Site 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Cell Periphery 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Cytoplasm 0 4.3 5.0 6.1 6.2 5.8 6.4 4.5 4.2 0 3.7 3.8 2.8 3.2 0
Endoplasmic Reticulum 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Endosome 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Golgi 0 0 0 0 0 0 0 0 0 0 0 0 3.5 5.6 4.5
Mitochondria 0 0 0 0 0 3.0 2.4 0 3.3 0 0 0 0 0 0
Nucleus -1.7 -5.9 -7.9 -10.8 -9.8 -8.7 -9.5 -7.3 -7.9 -1.5 -4.0 -4.5 -8.9 -8.3 -9.2
Nuclear Periphery 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Nucleolus 0 0 0 0 0 0 0 1.8 0 0 0 0 0 0 0
Peroxisomes 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
SpindlePole 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Vacuole -0.2 3.9 5.0 6.4 3.8 2.6 2.4 3.0 3.3 0.7 1.0 2.1 7.6 5.4 7.4