Standard name
Description Phospholipid translocase (flippase), role in phospholipid asymmetry of plasma membrane; involved in endocytosis, vacuolar biogenesis and Golgi to ER vesicle-mediated transport; localizes to endosomes and the Golgi apparatus

Micrographs

ensLOC DeepLoc
Localization WT1 WT2 WT3 RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3 WT1 WT2 WT3 AF100 AF140 AF180
Cortical Patches 0.09 0.08 0 0 0 0.05 0 0 0 0
Bud 0 0 0 0 0 0 0 0 0 0
Bud Neck 0 0 0 0 0 0 0 0 0 0
Bud Site 0 0 0 0
Cell Periphery 0 0 0 0 0 0 0 0 0 0
Cytoplasm 0.29 0.54 0.79 0.91 0 0.13 0.2 0.07 0.13 0.13
Endoplasmic Reticulum 0.58 0.44 0.05 0.05 0.54 0.44 0.39 0.66 0.57 0.52
Endosome 0.12 0 0.08 0.05 0.15 0.13 0.12 0.07 0.09 0.15
Golgi 0 0 0.05 0 0.14 0.1 0.06 0 0.08 0.05
Mitochondria 0 0 0 0 0 0.05 0 0 0 0
Nucleus 0 0 0 0.05 0 0 0 0 0 0
Nuclear Periphery 0 0 0 0 0 0 0 0 0 0
Nucleolus 0 0 0 0 0 0 0 0 0 0
Peroxisomes 0 0 0 0 0 0 0 0 0 0
SpindlePole 0 0 0 0 0 0 0 0 0 0
Vac/Vac Membrane 0.09 0 0.1 0.05 0.05 0.06 0.09 0 0.07 0.08
ensLOC DeepLoc
Localization WT1 WT2 WT3 RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3 WT1 WT2 WT3 AF100 AF140 AF180
Cortical Patches 16 17 0 0 7 13 2 0 1 0
Bud 1 5 0 0 0 1 1 0 0 0
Bud Neck 0 0 0 0 1 2 0 0 0 0
Bud Site 0 0 0 0
Cell Periphery 1 0 0 0 0 0 0 0 0 0
Cytoplasm 54 117 48 20 7 32 15 2 6 4
Endoplasmic Reticulum 108 95 3 1 118 111 31 19 29 19
Endosome 22 6 5 1 32 32 9 2 4 5
Golgi 8 3 3 0 30 24 4 1 4 1
Mitochondria 1 8 0 0 4 13 1 0 0 0
Nucleus 0 1 0 1 0 1 0 0 0 0
Nuclear Periphery 0 0 0 0 1 0 1 0 0 0
Nucleolus 0 0 1 0 0 0 0 0 0 0
Peroxisomes 0 0 0 0 1 0 0 0 0 0
SpindlePole 2 0 0 0 1 1 0 0 0 0
Vac/Vac Membrane 17 4 6 1 10 15 7 0 3 3
Unique Cell Count 187 217 61 22 219 255 80 29 52 38
Labelled Cell Count 230 256 66 24 219 255 80 29 52 38


Early Golgi

(Huh et al. Nature 2003 425:686)
Screen WT1 WT2 WT3 RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3 AF100 AF140 AF180
Mean Cell GFP Intensity (1e-4) 10.1 9.4 9.5 9.8 12.7
Std Deviation (1e-4) 1.3 1.9 2.0 2.5 3.6
Intensity Change (Log2) 0.05 0.42


Localization RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3
Cortical Patches 0
Bud 0
Bud Neck 0
Bud Site 0
Cell Periphery 0
Cytoplasm 1.3
Endoplasmic Reticulum 0
Endosome 0
Golgi 0
Mitochondria 0
Nucleus 0
Nuclear Periphery 0
Nucleolus 0
Peroxisomes 0
SpindlePole 0
Vacuole 0


Endocytosis
Temp Actin Patch (Sac6-tdTomato) Cortical Patch (Sla1-GFP) Late Endosome (Snf7-GFP) Vacuole (Vph1-GFP)
37℃
RT
Cell Cycle Omics

CYCLoPs (Neo1-GFP)
Gene / Allele Actin Patch (Sac6-tdTomato) Cortical Patch (Sla1-GFP) Late Endosome (Snf7-GFP) Vacuole (Sac6-tdTomato)
Gene Images
Gene Images
Images are not yet available
Images are not yet available