Standard name
Description Regulatory subunit of the cyclic AMP-dependent protein kinase (PKA); PKA is a component of a signaling pathway that controls a variety of cellular processes, including metabolism, cell cycle, stress response, stationary phase, and sporulation

Micrographs

Sub-cellular Localization

ensLOC DeepLoc
Localization WT1 WT2 WT3 RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3 WT1 WT2 WT3 AF100 AF140 AF180
Cortical Patches 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Bud 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Bud Neck 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Bud Site 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Cell Periphery 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Cytoplasm 0.53 0.21 0.12 0.23 0.17 0.28 0.29 0.30 0.32 0.24 0.24 0.47 0.33 0.39 0.34 0.26 0.29 0.22 0.43 0.14 0.06 0.11 0.11 0.10
Endoplasmic Reticulum 0 0 0 0 0 0 0 0 0.05 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Endosome 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Golgi 0 0 0.11 0.12 0.05 0.11 0.06 0.11 0.07 0.09 0 0 0.06 0.12 0.11 0.11 0 0.10 0 0 0 0 0 0
Mitochondria 0 0 0 0 0 0 0.06 0.05 0 0.10 0.31 0.15 0 0 0 0 0 0 0 0 0 0 0 0
Nucleus 0.26 0.58 0.65 0.55 0.49 0.34 0.28 0.19 0.19 0.22 0.14 0.11 0.42 0.39 0.34 0.44 0.42 0.46 0.39 0.68 0.64 0.69 0.57 0.50
Nuclear Periphery 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Nucleolus 0 0 0 0 0 0 0.08 0.06 0 0.05 0.08 0 0 0 0 0 0 0 0 0.07 0.08 0.05 0.11 0.13
Peroxisomes 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
SpindlePole 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Vac/Vac Membrane 0.21 0.22 0.18 0.18 0.29 0.33 0.31 0.38 0.35 0.34 0.24 0.30 0.21 0.19 0.26 0.26 0.28 0.27 0.05 0.06 0.12 0.09 0.15 0.21
ensLOC DeepLoc
Localization WT1 WT2 WT3 RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3 WT1 WT2 WT3 AF100 AF140 AF180
Cortical Patches 0 0 0 0 1 0 0 1 1 4 1 0 1 2 0 0 1 0 1 0 0 0 0 0
Bud 2 1 0 0 0 0 1 2 2 2 0 2 0 0 0 0 1 0 23 5 5 7 5 10
Bud Neck 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 2 0 0 0 1 0
Bud Site 0 0 0 0 0 0 1 1 1 2 0 1 0 0 0 0 0 0
Cell Periphery 0 0 1 3 2 2 3 1 4 1 3 3 3 5 0 7 3 3 0 0 0 0 0 0
Cytoplasm 245 44 16 24 14 38 46 52 48 53 32 54 89 103 121 101 106 57 229 37 10 22 30 33
Endoplasmic Reticulum 0 0 0 0 0 1 1 2 7 2 5 1 0 0 0 11 9 7 1 0 0 0 0 0
Endosome 4 2 1 0 0 1 0 1 0 1 0 0 2 2 2 1 0 0 6 2 2 1 2 4
Golgi 0 3 15 12 4 15 10 19 11 19 4 4 17 31 38 45 16 26 0 0 2 1 0 0
Mitochondria 6 1 0 1 1 5 9 9 6 22 41 17 0 1 1 3 2 0 0 0 1 0 0 0
Nucleus 123 123 91 57 41 46 44 34 29 49 18 13 113 102 122 172 152 122 203 179 115 142 159 175
Nuclear Periphery 0 0 0 0 0 0 0 0 0 0 0 1 0 0 0 0 0 0 0 0 0 0 0 1
Nucleolus 15 5 4 2 2 3 13 10 5 12 10 3 5 4 12 13 13 9 15 17 14 9 30 44
Peroxisomes 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
SpindlePole 0 0 0 1 0 0 0 1 0 0 0 0 0 0 0 1 0 0 13 1 2 0 4 4
Vac/Vac Membrane 97 47 25 19 24 45 49 66 52 75 31 34 55 49 92 101 102 71 24 16 21 19 41 72
Unique Cell Count 465 213 139 104 84 137 157 175 150 219 131 115 267 263 357 394 364 264 528 263 179 207 282 351
Labelled Cell Count 492 226 153 119 89 156 177 199 166 242 145 133 285 299 388 455 405 295 528 263 179 207 282 351

Yeast GFP Assignment

(Huh et al. Nature 2003 425:686 )

Cytoplasm, Nucleus

Protein Abundance

Screen WT1 WT2 WT3 RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3 AF100 AF140 AF180
Mean Cell GFP Intensity (1e-4) 12.6 25.9 23.1 21.9 19.8 22.5 20.4 20.0 20.9 18.6 17.2 20.0 21.5 21.2 20.8 42.3 38.5 41.9 22.2 22.8 24.8
Std Deviation (1e-4) 5.0 10.0 8.5 7.9 6.4 15.2 7.8 6.8 7.1 6.5 5.8 8.1 8.1 7.3 7.1 19.3 16.8 19.2 7.9 7.6 8.2
Intensity Change (Log2) -0.08 -0.22 -0.04 -0.18 -0.2 -0.14 -0.31 -0.43 -0.2 -0.1 -0.12 -0.15 0.87 0.74 0.86 -0.06 -0.01 0.11

Localization Change

Localization RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3
Cortical Patches 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Bud 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Bud Neck 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Bud Site 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Cell Periphery 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Cytoplasm 1.6 0 0.5 1.2 2.8 3.1 2.2 2.9 3.1 3.1 4.3 1.8 1.6 2.5 1.1
Endoplasmic Reticulum 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Endosome 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Golgi 0.2 -1.6 0 -1.4 0 -1.0 -0.7 -2.5 -2.2 -1.6 0.3 0 0.2 -2.7 -0.3
Mitochondria 0 0 0 0 0 0 3.9 7.2 4.7 0 0 0 0 0 0
Nucleus 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Nuclear Periphery 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Nucleolus 0 0 0 2.0 1.2 0 1.2 1.8 0 0 0 0 0 0 0
Peroxisomes 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
SpindlePole 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Vacuole 0 -0.1 0.2 -0.7 -0.6 -2.2 0 -1.9 -2.8 0.2 0.2 -0.3 -0.3 -0.2 0