Standard name
Description Regulatory subunit of the cyclic AMP-dependent protein kinase (PKA); PKA is a component of a signaling pathway that controls a variety of cellular processes, including metabolism, cell cycle, stress response, stationary phase, and sporulation
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Micrographs

ensLOC DeepLoc
Localization WT1 WT2 WT3 RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3 WT1 WT2 WT3 AF100 AF140 AF180
Cortical Patches 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Bud 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Bud Neck 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Bud Site 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 – – – – – –
Cell Periphery 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Cytoplasm 0.53 0.21 0.12 0.23 0.17 0.28 0.29 0.3 0.32 0.24 0.24 0.47 0.33 0.39 0.34 0.26 0.29 0.22 0.43 0.14 0.06 0.11 0.11 0.1
Endoplasmic Reticulum 0 0 0 0 0 0 0 0 0.05 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Endosome 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Golgi 0 0 0.11 0.12 0.05 0.11 0.06 0.11 0.07 0.09 0 0 0.06 0.12 0.11 0.11 0 0.1 0 0 0 0 0 0
Mitochondria 0 0 0 0 0 0 0.06 0.05 0 0.1 0.31 0.15 0 0 0 0 0 0 0 0 0 0 0 0
Nucleus 0.26 0.58 0.65 0.55 0.49 0.34 0.28 0.19 0.19 0.22 0.14 0.11 0.42 0.39 0.34 0.44 0.42 0.46 0.39 0.68 0.64 0.69 0.57 0.5
Nuclear Periphery 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Nucleolus 0 0 0 0 0 0 0.08 0.06 0 0.05 0.08 0 0 0 0 0 0 0 0 0.07 0.08 0.05 0.11 0.13
Peroxisomes 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
SpindlePole 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Vac/Vac Membrane 0.21 0.22 0.18 0.18 0.29 0.33 0.31 0.38 0.35 0.34 0.24 0.3 0.21 0.19 0.26 0.26 0.28 0.27 0.05 0.06 0.12 0.09 0.15 0.21
ensLOC DeepLoc
Localization WT1 WT2 WT3 RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3 WT1 WT2 WT3 AF100 AF140 AF180
Cortical Patches 0 0 0 0 1 0 0 1 1 4 1 0 1 2 0 0 1 0 1 0 0 0 0 0
Bud 2 1 0 0 0 0 1 2 2 2 0 2 0 0 0 0 1 0 23 5 5 7 5 10
Bud Neck 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 2 0 0 0 1 0
Bud Site 0 0 0 0 0 0 1 1 1 2 0 1 0 0 0 0 0 0 – – – – – –
Cell Periphery 0 0 1 3 2 2 3 1 4 1 3 3 3 5 0 7 3 3 0 0 0 0 0 0
Cytoplasm 245 44 16 24 14 38 46 52 48 53 32 54 89 103 121 101 106 57 229 37 10 22 30 33
Endoplasmic Reticulum 0 0 0 0 0 1 1 2 7 2 5 1 0 0 0 11 9 7 1 0 0 0 0 0
Endosome 4 2 1 0 0 1 0 1 0 1 0 0 2 2 2 1 0 0 6 2 2 1 2 4
Golgi 0 3 15 12 4 15 10 19 11 19 4 4 17 31 38 45 16 26 0 0 2 1 0 0
Mitochondria 6 1 0 1 1 5 9 9 6 22 41 17 0 1 1 3 2 0 0 0 1 0 0 0
Nucleus 123 123 91 57 41 46 44 34 29 49 18 13 113 102 122 172 152 122 203 179 115 142 159 175
Nuclear Periphery 0 0 0 0 0 0 0 0 0 0 0 1 0 0 0 0 0 0 0 0 0 0 0 1
Nucleolus 15 5 4 2 2 3 13 10 5 12 10 3 5 4 12 13 13 9 15 17 14 9 30 44
Peroxisomes 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
SpindlePole 0 0 0 1 0 0 0 1 0 0 0 0 0 0 0 1 0 0 13 1 2 0 4 4
Vac/Vac Membrane 97 47 25 19 24 45 49 66 52 75 31 34 55 49 92 101 102 71 24 16 21 19 41 72
Unique Cell Count 465 213 139 104 84 137 157 175 150 219 131 115 267 263 357 394 364 264 528 263 179 207 282 351
Labelled Cell Count 492 226 153 119 89 156 177 199 166 242 145 133 285 299 388 455 405 295 528 263 179 207 282 351


Cytoplasm, Nucleus

(Huh et al. Nature 2003 425:686)
Screen WT1 WT2 WT3 RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3 AF100 AF140 AF180
Mean Cell GFP Intensity (1e-4) 12.6 25.9 23.1 21.9 19.8 22.5 20.4 20.0 20.9 18.6 17.2 20.0 21.5 21.2 20.8 42.3 38.5 41.9 22.2 22.8 24.8
Std Deviation (1e-4) 5.0 10.0 8.5 7.9 6.4 15.2 7.8 6.8 7.1 6.5 5.8 8.1 8.1 7.3 7.1 19.3 16.8 19.2 7.9 7.6 8.2
Intensity Change (Log2) – – – -0.08 -0.22 -0.04 -0.18 -0.2 -0.14 -0.31 -0.43 -0.2 -0.1 -0.12 -0.15 0.87 0.74 0.86 -0.06 -0.01 0.11


Localization RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3
Cortical Patches 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Bud 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Bud Neck 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Bud Site 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Cell Periphery 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Cytoplasm 2.4 1.1 3.4 3.8 3.9 4.2 3.0 2.8 6.3 4.8 5.8 5.0 3.5 4.1 2.5
Endoplasmic Reticulum 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Endosome 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Golgi 0.2 -1.6 0 -1.4 0 -1.0 -0.7 -2.5 -2.2 -1.6 0.3 0 0.2 -2.7 -0.3
Mitochondria 0 0 0 0 0 0 3.9 7.2 4.7 0 0 0 0 0 0
Nucleus -1.7 -2.5 -5.3 -6.5 -8.3 -8.0 -8.1 -8.7 -8.7 -4.4 -5.1 -6.3 -4.4 -4.8 -3.7
Nuclear Periphery 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Nucleolus 0 0 0 2.0 1.2 0 1.2 1.8 0 0 0 0 0 0 0
Peroxisomes 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
SpindlePole 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Vacuole 0.1 1.8 2.8 2.6 3.8 3.2 3.3 1.2 2.2 0.6 0.2 1.8 1.8 2.3 2.0

Images

Circle plot not available

Protein Concentration and Protein Localization Data

R1 R2 R3
G1 Pre-START G1 Post-START S/G2 Metaphase Anaphase Telophase G1 Pre-START G1 Post-START S/G2 Metaphase Anaphase Telophase G1 Pre-START G1 Post-START S/G2 Metaphase Anaphase Telophase
Concentration 7.9688 10.8948 9.3919 7.9661 10.1366 9.1858 12.5585 14.6536 13.3224 13.3141 12.9539 13.6058 13.2716 14.4907 13.9215 12.7089 11.6849 13.23
Actin 0.0004 0.0002 0.0006 0.0001 0.0008 0.0002 0.0085 0.0006 0.0044 0.001 0.0062 0.0007 0.0148 0.0005 0.0018 0.0094 0.0002 0.0003
Bud 0.0002 0.0014 0.0011 0.0001 0.0002 0.0001 0.0025 0.0016 0.0037 0.003 0.0039 0.0019 0.0001 0.0033 0.0057 0.0003 0.0001 0.0002
Bud Neck 0.0005 0.0004 0.0003 0.0005 0.0007 0.001 0.0043 0.0005 0.0013 0.002 0.001 0.0019 0.0002 0.0003 0.0005 0.0007 0.0006 0.0007
Bud Periphery 0.0001 0.0003 0.0014 0 0 0.0001 0.0013 0.0005 0.0029 0.0024 0.0021 0.0012 0 0.0009 0.0011 0.0002 0 0
Bud Site 0.0004 0.0059 0.0009 0.0001 0.0002 0.0001 0.0031 0.0022 0.0044 0.0004 0.0009 0.0002 0.0001 0.0011 0.001 0.0005 0 0.0001
Cell Periphery 0.0001 0.0001 0.0002 0 0 0 0.0003 0.0001 0.0003 0.0001 0.0002 0 0 0.0002 0.0001 0.0001 0 0
Cytoplasm 0.2738 0.4208 0.3808 0.3005 0.3856 0.3604 0.2169 0.484 0.2981 0.3539 0.1959 0.3318 0.5188 0.7368 0.5928 0.649 0.6052 0.6608
Cytoplasmic Foci 0.0043 0.0017 0.0048 0.0064 0.0084 0.0035 0.0155 0.004 0.0071 0.0014 0.0121 0.0026 0.0007 0.0027 0.0032 0.0042 0.0018 0.0025
Eisosomes 0 0 0 0 0 0 0.0001 0 0.0002 0 0.0001 0 0.0001 0 0 0.0002 0 0
Endoplasmic Reticulum 0.0085 0.001 0.0038 0.0022 0.0041 0.0023 0.006 0.0015 0.003 0.0013 0.0057 0.0015 0.0015 0.0014 0.002 0.0036 0.0022 0.0085
Endosome 0.0152 0.0011 0.0083 0.016 0.0182 0.018 0.0238 0.0022 0.0091 0.0014 0.0209 0.0053 0.0008 0.0019 0.0039 0.0035 0.0025 0.0105
Golgi 0.0004 0.0001 0.0006 0.0001 0.0002 0.0003 0.0041 0.0003 0.0017 0.0001 0.0014 0.0002 0.0002 0.0002 0.0004 0.0008 0.0001 0.0004
Lipid Particles 0.0005 0 0.0002 0.0001 0.0001 0.0002 0.0089 0.0003 0.0044 0.0001 0.007 0.0002 0 0 0.0001 0.001 0 0
Mitochondria 0.0004 0.0002 0.0018 0.0002 0.0002 0.0004 0.0065 0.0019 0.007 0.0012 0.0022 0.0003 0.0003 0.0004 0.0006 0.0015 0.0002 0.0005
None 0.003 0.0011 0.0099 0.0017 0.001 0.0049 0.0035 0.001 0.0015 0.0012 0.012 0.0009 0.0009 0.0014 0.0016 0.0018 0.0012 0.0006
Nuclear Periphery 0.0375 0.0131 0.0134 0.0238 0.0151 0.0236 0.0245 0.0098 0.014 0.011 0.0174 0.0096 0.0174 0.0057 0.0141 0.0173 0.0117 0.0078
Nucleolus 0.0011 0.0006 0.0019 0.0007 0.0005 0.0011 0.0035 0.0016 0.0027 0.0017 0.0028 0.0018 0.0001 0.0017 0.0003 0.0014 0.0001 0.0025
Nucleus 0.6397 0.5435 0.5629 0.6386 0.5531 0.5751 0.6397 0.4823 0.6118 0.6051 0.639 0.632 0.4424 0.2364 0.3649 0.2971 0.3706 0.3011
Peroxisomes 0.0001 0 0.0002 0 0.0002 0 0.0087 0.0005 0.0023 0.0001 0.0012 0.0001 0 0.0001 0.0001 0.0002 0 0
Punctate Nuclear 0.006 0.0056 0.0029 0.0027 0.004 0.0043 0.0123 0.0025 0.0155 0.0102 0.0638 0.0063 0.0009 0.0037 0.0038 0.0048 0.0016 0.0011
Vacuole 0.0067 0.0028 0.0031 0.0051 0.0051 0.0033 0.0042 0.0022 0.0035 0.0022 0.0031 0.0012 0.0005 0.001 0.0015 0.0016 0.0015 0.0018
Vacuole Periphery 0.0011 0.0002 0.0008 0.0011 0.002 0.0013 0.0017 0.0003 0.001 0.0005 0.0012 0.0003 0.0002 0.0002 0.0004 0.0011 0.0004 0.0006

Sequencing Data

R1 R2
G1 Post-START S/G2 Metaphase Anaphase Telophase G1 Post-START S/G2 Metaphase Anaphase Telophase
Gene Expression 50.716 42.5774 56.7527 64.7403 53.1762 46.7211 55.6062 56.4974 63.8976 60.2568
Translational Efficiency 1.7434 1.9386 1.6449 1.6521 1.8965 2.8061 1.6136 1.9634 2.0663 1.6936

Hit Data

Dataset Hit
Protein Abundance ✘
Protein Localization ✘
Gene Expression ✘
Translational Efficiency ✘