Standard name | |
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Description | Regulatory subunit of the cyclic AMP-dependent protein kinase (PKA); PKA is a component of a signaling pathway that controls a variety of cellular processes, including metabolism, cell cycle, stress response, stationary phase, and sporulation |
ensLOC | DeepLoc | |||||||||||||||||||||||
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Localization | WT1 | WT2 | WT3 | RAP60 | RAP140 | RAP220 | RAP300 | RAP380 | RAP460 | RAP540 | RAP620 | RAP700 | HU80 | HU120 | HU160 | rpd3Δ_1 | rpd3Δ_2 | rpd3Δ_3 | WT1 | WT2 | WT3 | AF100 | AF140 | AF180 |
Cortical Patches | 0 | 0 | 0 | 0 | 1 | 0 | 0 | 1 | 1 | 4 | 1 | 0 | 1 | 2 | 0 | 0 | 1 | 0 | 1 | 0 | 0 | 0 | 0 | 0 |
Bud | 2 | 1 | 0 | 0 | 0 | 0 | 1 | 2 | 2 | 2 | 0 | 2 | 0 | 0 | 0 | 0 | 1 | 0 | 23 | 5 | 5 | 7 | 5 | 10 |
Bud Neck | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 2 | 0 | 0 | 0 | 1 | 0 |
Bud Site | 0 | 0 | 0 | 0 | 0 | 0 | 1 | 1 | 1 | 2 | 0 | 1 | 0 | 0 | 0 | 0 | 0 | 0 | – | – | – | – | – | – |
Cell Periphery | 0 | 0 | 1 | 3 | 2 | 2 | 3 | 1 | 4 | 1 | 3 | 3 | 3 | 5 | 0 | 7 | 3 | 3 | 0 | 0 | 0 | 0 | 0 | 0 |
Cytoplasm | 245 | 44 | 16 | 24 | 14 | 38 | 46 | 52 | 48 | 53 | 32 | 54 | 89 | 103 | 121 | 101 | 106 | 57 | 229 | 37 | 10 | 22 | 30 | 33 |
Endoplasmic Reticulum | 0 | 0 | 0 | 0 | 0 | 1 | 1 | 2 | 7 | 2 | 5 | 1 | 0 | 0 | 0 | 11 | 9 | 7 | 1 | 0 | 0 | 0 | 0 | 0 |
Endosome | 4 | 2 | 1 | 0 | 0 | 1 | 0 | 1 | 0 | 1 | 0 | 0 | 2 | 2 | 2 | 1 | 0 | 0 | 6 | 2 | 2 | 1 | 2 | 4 |
Golgi | 0 | 3 | 15 | 12 | 4 | 15 | 10 | 19 | 11 | 19 | 4 | 4 | 17 | 31 | 38 | 45 | 16 | 26 | 0 | 0 | 2 | 1 | 0 | 0 |
Mitochondria | 6 | 1 | 0 | 1 | 1 | 5 | 9 | 9 | 6 | 22 | 41 | 17 | 0 | 1 | 1 | 3 | 2 | 0 | 0 | 0 | 1 | 0 | 0 | 0 |
Nucleus | 123 | 123 | 91 | 57 | 41 | 46 | 44 | 34 | 29 | 49 | 18 | 13 | 113 | 102 | 122 | 172 | 152 | 122 | 203 | 179 | 115 | 142 | 159 | 175 |
Nuclear Periphery | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 1 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 1 |
Nucleolus | 15 | 5 | 4 | 2 | 2 | 3 | 13 | 10 | 5 | 12 | 10 | 3 | 5 | 4 | 12 | 13 | 13 | 9 | 15 | 17 | 14 | 9 | 30 | 44 |
Peroxisomes | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
SpindlePole | 0 | 0 | 0 | 1 | 0 | 0 | 0 | 1 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 1 | 0 | 0 | 13 | 1 | 2 | 0 | 4 | 4 |
Vac/Vac Membrane | 97 | 47 | 25 | 19 | 24 | 45 | 49 | 66 | 52 | 75 | 31 | 34 | 55 | 49 | 92 | 101 | 102 | 71 | 24 | 16 | 21 | 19 | 41 | 72 |
Unique Cell Count | 465 | 213 | 139 | 104 | 84 | 137 | 157 | 175 | 150 | 219 | 131 | 115 | 267 | 263 | 357 | 394 | 364 | 264 | 528 | 263 | 179 | 207 | 282 | 351 |
Labelled Cell Count | 492 | 226 | 153 | 119 | 89 | 156 | 177 | 199 | 166 | 242 | 145 | 133 | 285 | 299 | 388 | 455 | 405 | 295 | 528 | 263 | 179 | 207 | 282 | 351 |
Screen | WT1 | WT2 | WT3 | RAP60 | RAP140 | RAP220 | RAP300 | RAP380 | RAP460 | RAP540 | RAP620 | RAP700 | HU80 | HU120 | HU160 | rpd3Δ_1 | rpd3Δ_2 | rpd3Δ_3 | AF100 | AF140 | AF180 |
---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|
Mean Cell GFP Intensity (1e-4) | 12.6 | 25.9 | 23.1 | 21.9 | 19.8 | 22.5 | 20.4 | 20.0 | 20.9 | 18.6 | 17.2 | 20.0 | 21.5 | 21.2 | 20.8 | 42.3 | 38.5 | 41.9 | 22.2 | 22.8 | 24.8 |
Std Deviation (1e-4) | 5.0 | 10.0 | 8.5 | 7.9 | 6.4 | 15.2 | 7.8 | 6.8 | 7.1 | 6.5 | 5.8 | 8.1 | 8.1 | 7.3 | 7.1 | 19.3 | 16.8 | 19.2 | 7.9 | 7.6 | 8.2 |
Intensity Change (Log2) | – | – | – | -0.08 | -0.22 | -0.04 | -0.18 | -0.2 | -0.14 | -0.31 | -0.43 | -0.2 | -0.1 | -0.12 | -0.15 | 0.87 | 0.74 | 0.86 | -0.06 | -0.01 | 0.11 |
Localization | RAP60 | RAP140 | RAP220 | RAP300 | RAP380 | RAP460 | RAP540 | RAP620 | RAP700 | HU80 | HU120 | HU160 | rpd3Δ_1 | rpd3Δ_2 | rpd3Δ_3 |
---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|
Cortical Patches | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
Bud | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
Bud Neck | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
Bud Site | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
Cell Periphery | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
Cytoplasm | 2.4 | 1.1 | 3.4 | 3.8 | 3.9 | 4.2 | 3.0 | 2.8 | 6.3 | 4.8 | 5.8 | 5.0 | 3.5 | 4.1 | 2.5 |
Endoplasmic Reticulum | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
Endosome | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
Golgi | 0.2 | -1.6 | 0 | -1.4 | 0 | -1.0 | -0.7 | -2.5 | -2.2 | -1.6 | 0.3 | 0 | 0.2 | -2.7 | -0.3 |
Mitochondria | 0 | 0 | 0 | 0 | 0 | 0 | 3.9 | 7.2 | 4.7 | 0 | 0 | 0 | 0 | 0 | 0 |
Nucleus | -1.7 | -2.5 | -5.3 | -6.5 | -8.3 | -8.0 | -8.1 | -8.7 | -8.7 | -4.4 | -5.1 | -6.3 | -4.4 | -4.8 | -3.7 |
Nuclear Periphery | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
Nucleolus | 0 | 0 | 0 | 2.0 | 1.2 | 0 | 1.2 | 1.8 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
Peroxisomes | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
SpindlePole | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
Vacuole | 0.1 | 1.8 | 2.8 | 2.6 | 3.8 | 3.2 | 3.3 | 1.2 | 2.2 | 0.6 | 0.2 | 1.8 | 1.8 | 2.3 | 2.0 |
R1 | R2 | R3 | ||||||||||||||||
---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|
G1 Pre-START | G1 Post-START | S/G2 | Metaphase | Anaphase | Telophase | G1 Pre-START | G1 Post-START | S/G2 | Metaphase | Anaphase | Telophase | G1 Pre-START | G1 Post-START | S/G2 | Metaphase | Anaphase | Telophase | |
Concentration | 7.9688 | 10.8948 | 9.3919 | 7.9661 | 10.1366 | 9.1858 | 12.5585 | 14.6536 | 13.3224 | 13.3141 | 12.9539 | 13.6058 | 13.2716 | 14.4907 | 13.9215 | 12.7089 | 11.6849 | 13.23 |
Actin | 0.0004 | 0.0002 | 0.0006 | 0.0001 | 0.0008 | 0.0002 | 0.0085 | 0.0006 | 0.0044 | 0.001 | 0.0062 | 0.0007 | 0.0148 | 0.0005 | 0.0018 | 0.0094 | 0.0002 | 0.0003 |
Bud | 0.0002 | 0.0014 | 0.0011 | 0.0001 | 0.0002 | 0.0001 | 0.0025 | 0.0016 | 0.0037 | 0.003 | 0.0039 | 0.0019 | 0.0001 | 0.0033 | 0.0057 | 0.0003 | 0.0001 | 0.0002 |
Bud Neck | 0.0005 | 0.0004 | 0.0003 | 0.0005 | 0.0007 | 0.001 | 0.0043 | 0.0005 | 0.0013 | 0.002 | 0.001 | 0.0019 | 0.0002 | 0.0003 | 0.0005 | 0.0007 | 0.0006 | 0.0007 |
Bud Periphery | 0.0001 | 0.0003 | 0.0014 | 0 | 0 | 0.0001 | 0.0013 | 0.0005 | 0.0029 | 0.0024 | 0.0021 | 0.0012 | 0 | 0.0009 | 0.0011 | 0.0002 | 0 | 0 |
Bud Site | 0.0004 | 0.0059 | 0.0009 | 0.0001 | 0.0002 | 0.0001 | 0.0031 | 0.0022 | 0.0044 | 0.0004 | 0.0009 | 0.0002 | 0.0001 | 0.0011 | 0.001 | 0.0005 | 0 | 0.0001 |
Cell Periphery | 0.0001 | 0.0001 | 0.0002 | 0 | 0 | 0 | 0.0003 | 0.0001 | 0.0003 | 0.0001 | 0.0002 | 0 | 0 | 0.0002 | 0.0001 | 0.0001 | 0 | 0 |
Cytoplasm | 0.2738 | 0.4208 | 0.3808 | 0.3005 | 0.3856 | 0.3604 | 0.2169 | 0.484 | 0.2981 | 0.3539 | 0.1959 | 0.3318 | 0.5188 | 0.7368 | 0.5928 | 0.649 | 0.6052 | 0.6608 |
Cytoplasmic Foci | 0.0043 | 0.0017 | 0.0048 | 0.0064 | 0.0084 | 0.0035 | 0.0155 | 0.004 | 0.0071 | 0.0014 | 0.0121 | 0.0026 | 0.0007 | 0.0027 | 0.0032 | 0.0042 | 0.0018 | 0.0025 |
Eisosomes | 0 | 0 | 0 | 0 | 0 | 0 | 0.0001 | 0 | 0.0002 | 0 | 0.0001 | 0 | 0.0001 | 0 | 0 | 0.0002 | 0 | 0 |
Endoplasmic Reticulum | 0.0085 | 0.001 | 0.0038 | 0.0022 | 0.0041 | 0.0023 | 0.006 | 0.0015 | 0.003 | 0.0013 | 0.0057 | 0.0015 | 0.0015 | 0.0014 | 0.002 | 0.0036 | 0.0022 | 0.0085 |
Endosome | 0.0152 | 0.0011 | 0.0083 | 0.016 | 0.0182 | 0.018 | 0.0238 | 0.0022 | 0.0091 | 0.0014 | 0.0209 | 0.0053 | 0.0008 | 0.0019 | 0.0039 | 0.0035 | 0.0025 | 0.0105 |
Golgi | 0.0004 | 0.0001 | 0.0006 | 0.0001 | 0.0002 | 0.0003 | 0.0041 | 0.0003 | 0.0017 | 0.0001 | 0.0014 | 0.0002 | 0.0002 | 0.0002 | 0.0004 | 0.0008 | 0.0001 | 0.0004 |
Lipid Particles | 0.0005 | 0 | 0.0002 | 0.0001 | 0.0001 | 0.0002 | 0.0089 | 0.0003 | 0.0044 | 0.0001 | 0.007 | 0.0002 | 0 | 0 | 0.0001 | 0.001 | 0 | 0 |
Mitochondria | 0.0004 | 0.0002 | 0.0018 | 0.0002 | 0.0002 | 0.0004 | 0.0065 | 0.0019 | 0.007 | 0.0012 | 0.0022 | 0.0003 | 0.0003 | 0.0004 | 0.0006 | 0.0015 | 0.0002 | 0.0005 |
None | 0.003 | 0.0011 | 0.0099 | 0.0017 | 0.001 | 0.0049 | 0.0035 | 0.001 | 0.0015 | 0.0012 | 0.012 | 0.0009 | 0.0009 | 0.0014 | 0.0016 | 0.0018 | 0.0012 | 0.0006 |
Nuclear Periphery | 0.0375 | 0.0131 | 0.0134 | 0.0238 | 0.0151 | 0.0236 | 0.0245 | 0.0098 | 0.014 | 0.011 | 0.0174 | 0.0096 | 0.0174 | 0.0057 | 0.0141 | 0.0173 | 0.0117 | 0.0078 |
Nucleolus | 0.0011 | 0.0006 | 0.0019 | 0.0007 | 0.0005 | 0.0011 | 0.0035 | 0.0016 | 0.0027 | 0.0017 | 0.0028 | 0.0018 | 0.0001 | 0.0017 | 0.0003 | 0.0014 | 0.0001 | 0.0025 |
Nucleus | 0.6397 | 0.5435 | 0.5629 | 0.6386 | 0.5531 | 0.5751 | 0.6397 | 0.4823 | 0.6118 | 0.6051 | 0.639 | 0.632 | 0.4424 | 0.2364 | 0.3649 | 0.2971 | 0.3706 | 0.3011 |
Peroxisomes | 0.0001 | 0 | 0.0002 | 0 | 0.0002 | 0 | 0.0087 | 0.0005 | 0.0023 | 0.0001 | 0.0012 | 0.0001 | 0 | 0.0001 | 0.0001 | 0.0002 | 0 | 0 |
Punctate Nuclear | 0.006 | 0.0056 | 0.0029 | 0.0027 | 0.004 | 0.0043 | 0.0123 | 0.0025 | 0.0155 | 0.0102 | 0.0638 | 0.0063 | 0.0009 | 0.0037 | 0.0038 | 0.0048 | 0.0016 | 0.0011 |
Vacuole | 0.0067 | 0.0028 | 0.0031 | 0.0051 | 0.0051 | 0.0033 | 0.0042 | 0.0022 | 0.0035 | 0.0022 | 0.0031 | 0.0012 | 0.0005 | 0.001 | 0.0015 | 0.0016 | 0.0015 | 0.0018 |
Vacuole Periphery | 0.0011 | 0.0002 | 0.0008 | 0.0011 | 0.002 | 0.0013 | 0.0017 | 0.0003 | 0.001 | 0.0005 | 0.0012 | 0.0003 | 0.0002 | 0.0002 | 0.0004 | 0.0011 | 0.0004 | 0.0006 |
R1 | R2 | |||||||||
---|---|---|---|---|---|---|---|---|---|---|
G1 Post-START | S/G2 | Metaphase | Anaphase | Telophase | G1 Post-START | S/G2 | Metaphase | Anaphase | Telophase | |
Gene Expression | 50.716 | 42.5774 | 56.7527 | 64.7403 | 53.1762 | 46.7211 | 55.6062 | 56.4974 | 63.8976 | 60.2568 |
Translational Efficiency | 1.7434 | 1.9386 | 1.6449 | 1.6521 | 1.8965 | 2.8061 | 1.6136 | 1.9634 | 2.0663 | 1.6936 |
Dataset | Hit |
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Protein Abundance | ✘ |
Protein Localization | ✘ |
Gene Expression | ✘ |
Translational Efficiency | ✘ |