Standard name
Description Enzyme that catalyzes the fourth step in the histidine pathway; Phosphoribosylformimino-5-aminoimidazole carboxamide ribotide isomerase; mutations cause histidine auxotrophy and sensitivity to Cu, Co, and Ni salts
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Micrographs

ensLOC DeepLoc
Localization WT1 WT2 WT3 RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3 WT1 WT2 WT3 AF100 AF140 AF180
Cortical Patches – 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Bud – 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Bud Neck – 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Bud Site – 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 – – – – – –
Cell Periphery – 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Cytoplasm – 0.95 0.97 0.92 0.97 0.92 0.85 0.88 0.72 0.78 0.71 0.68 0.98 0.98 1.0 0.97 0.97 0.95 1.0 0.94 0.89 0.86 0.93 0.94
Endoplasmic Reticulum – 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Endosome – 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Golgi – 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Mitochondria – 0 0 0 0 0.06 0.14 0.09 0.38 0.34 0.36 0.34 0 0 0 0 0 0 0 0 0 0 0 0
Nucleus – 0 0 0 0 0 0 0 0 0 0 0 0.05 0 0 0 0 0 0 0 0 0 0 0
Nuclear Periphery – 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Nucleolus – 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Peroxisomes – 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
SpindlePole – 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Vac/Vac Membrane – 0 0 0.05 0.06 0 0.06 0.07 0 0.05 0.05 0.06 0 0 0 0 0 0.05 0 0 0 0 0 0
ensLOC DeepLoc
Localization WT1 WT2 WT3 RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3 WT1 WT2 WT3 AF100 AF140 AF180
Cortical Patches – 0 0 0 0 0 0 0 0 0 0 1 0 0 0 0 0 0 0 1 0 0 0 0
Bud – 1 0 0 1 0 1 0 0 1 4 4 0 0 0 0 0 0 0 0 0 0 1 1
Bud Neck – 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Bud Site – 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 – – – – – –
Cell Periphery – 0 1 2 3 0 3 2 0 2 1 0 0 1 1 1 0 1 0 0 0 0 0 0
Cytoplasm – 122 108 103 221 159 173 203 130 134 105 107 62 226 254 122 97 106 15 128 101 108 160 220
Endoplasmic Reticulum – 2 0 0 1 1 0 0 0 0 0 3 0 4 0 3 3 1 0 0 0 4 1 1
Endosome – 0 0 0 1 0 0 1 0 0 1 0 0 0 0 1 0 0 0 2 1 2 2 0
Golgi – 0 0 0 0 0 1 0 0 0 0 0 0 0 0 0 0 0 0 0 0 1 0 0
Mitochondria – 5 0 1 1 10 29 21 68 58 53 53 0 0 0 3 0 1 0 1 1 0 0 1
Nucleus – 0 1 1 0 5 3 1 2 6 4 3 3 1 0 3 1 3 0 0 1 1 1 0
Nuclear Periphery – 1 0 2 0 0 1 0 0 2 1 1 0 0 0 0 0 0 0 0 0 0 0 0
Nucleolus – 0 0 0 0 1 0 1 4 0 0 1 0 0 0 0 0 0 0 0 0 0 0 0
Peroxisomes – 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
SpindlePole – 0 0 0 0 0 1 0 1 0 0 0 0 0 0 0 0 0 0 0 0 0 0 1
Vac/Vac Membrane – 1 3 6 13 7 12 17 7 9 8 9 0 0 2 2 4 6 0 0 4 2 2 3
Unique Cell Count 129 111 112 227 173 204 231 180 171 148 157 63 230 254 126 100 112 16 137 114 126 174 234
Labelled Cell Count 132 113 115 241 183 224 246 212 212 177 182 65 232 257 135 105 118 16 137 114 126 174 234


Cytoplasm, Nucleus

(Huh et al. Nature 2003 425:686)
Screen WT1 WT2 WT3 RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3 AF100 AF140 AF180
Mean Cell GFP Intensity (1e-4) – 5.8 8.1 8.1 7.8 6.3 5.7 5.8 4.8 5.3 5.1 4.9 6.2 8.7 8.2 10.9 12.4 14.1 10.3 9.2 8.8
Std Deviation (1e-4) – 1.0 2.1 3.1 1.9 1.9 1.5 1.6 1.7 2.4 2.1 1.3 1.5 2.1 2.1 2.0 2.5 3.1 2.9 2.0 2.4
Intensity Change (Log2) – – – -0.0 -0.05 -0.35 -0.5 -0.47 -0.76 -0.61 -0.67 -0.72 -0.38 0.09 0.01 0.43 0.61 0.79 0.35 0.18 0.12


Localization RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3
Cortical Patches 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Bud 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Bud Neck 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Bud Site 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Cell Periphery 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Cytoplasm -1.8 0 -1.9 -3.4 -2.8 -5.4 -4.5 -5.5 -5.9 0.5 0.6 2.6 -0.2 -0.1 -1.0
Endoplasmic Reticulum 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Endosome 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Golgi 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Mitochondria 0 0 0 4.2 3.3 0 6.9 7.1 6.8 0 0 0 0 0 0
Nucleus 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Nuclear Periphery 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Nucleolus 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Peroxisomes 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
SpindlePole 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Vacuole 0 1.2 0 1.3 1.7 0 0 0 0 0 0 0 0 0 0