Standard name
Description Cytosolic J-domain-containing protein; required for peroxisomal protein import and involved in peroxisome assembly; facilitates import of Mim1p and Mim2p into the mitochondrial outer membrane; homologous to E. coli DnaJ

Micrographs

ensLOC DeepLoc
Localization WT1 WT2 WT3 RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3 WT1 WT2 WT3 AF100 AF140 AF180
Cortical Patches 0 0 0 0.07 0 0 0 0 0 0 0 0 0 0 0 0.11 0 0 0 0 0 0 0
Bud 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Bud Neck 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Bud Site 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Cell Periphery 0 0 0.05 0 0 0 0 0 0 0 0.05 0.06 0 0.09 0.08 0 0.14 0 0 0 0 0 0
Cytoplasm 0.08 0.2 0.07 0.15 0.12 0.21 0.18 0.16 0.17 0.21 0.21 0.24 0.06 0.14 0.25 0.72 0.77 0 0.06 0 0 0 0
Endoplasmic Reticulum 0.86 0.71 0.9 0.68 0.7 0.69 0.47 0.36 0.42 0.28 0.35 0.25 0.92 0.83 0.71 0 0 0.96 0.81 0.94 0.9 0.89 0.87
Endosome 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Golgi 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Mitochondria 0 0.05 0 0.12 0 0.1 0.13 0.18 0.31 0.3 0.38 0.27 0 0 0 0 0 0 0 0 0 0 0
Nucleus 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0.11 0 0 0 0 0 0 0
Nuclear Periphery 0 0 0.05 0.08 0 0 0.06 0.07 0.11 0 0.11 0.06 0 0 0 0 0 0 0 0 0 0 0
Nucleolus 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Peroxisomes 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
SpindlePole 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Vac/Vac Membrane 0.08 0 0.05 0.16 0.18 0.1 0.3 0.36 0.17 0.27 0.19 0.32 0 0 0 0.11 0.09 0 0 0 0 0 0
ensLOC DeepLoc
Localization WT1 WT2 WT3 RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3 WT1 WT2 WT3 AF100 AF140 AF180
Cortical Patches 2 7 5 5 5 1 5 7 2 4 3 2 1 3 3 2 0 0 4 1 2 8 6
Bud 0 2 0 1 0 0 4 3 2 4 3 0 0 0 0 0 0 0 2 0 1 2 2
Bud Neck 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 1 3
Bud Site 0 0 0 0 0 0 0 0 0 1 0 0 0 0 0 0 0
Cell Periphery 1 6 11 1 6 3 8 8 7 7 12 15 4 21 15 0 3 0 1 2 0 1 6
Cytoplasm 12 32 16 11 16 29 41 51 32 48 53 63 6 35 49 13 17 2 12 1 2 4 10
Endoplasmic Reticulum 124 115 200 51 96 93 109 114 76 64 88 67 94 204 142 0 0 153 156 209 145 352 330
Endosome 0 0 1 1 4 3 4 6 0 1 2 0 0 1 0 0 0 0 0 0 1 1 2
Golgi 0 1 1 3 1 0 1 1 0 0 0 0 0 0 0 0 0 0 0 1 0 1 2
Mitochondria 5 8 0 9 5 14 31 58 57 70 97 73 0 0 0 0 0 1 2 2 1 3 2
Nucleus 0 2 0 0 0 1 1 4 2 6 3 3 1 0 2 2 0 0 0 0 0 0 0
Nuclear Periphery 1 5 12 6 3 5 13 21 20 8 27 17 4 4 0 0 0 1 2 2 1 3 1
Nucleolus 0 0 0 0 0 0 0 0 2 1 0 1 0 0 0 0 0 0 0 0 0 0 0
Peroxisomes 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
SpindlePole 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Vac/Vac Membrane 11 3 10 12 25 13 70 113 31 62 49 85 2 9 7 2 2 0 6 0 1 8 9
Unique Cell Count 144 161 221 75 137 135 234 318 183 230 252 268 102 245 199 18 22 159 193 222 161 395 380
Labelled Cell Count 156 181 256 100 161 162 287 386 231 276 337 326 112 277 218 19 22 159 193 222 161 395 380


Endoplasmic Reticulum

(Huh et al. Nature 2003 425:686)
Screen WT1 WT2 WT3 RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3 AF100 AF140 AF180
Mean Cell GFP Intensity (1e-4) 11.3 9.3 12.1 11.5 11.1 9.2 8.9 8.7 8.4 7.4 7.5 8.1 11.7 12.3 12.5 11.0 11.3 12.2 13.2 14.0
Std Deviation (1e-4) 1.9 1.6 1.9 2.2 1.9 1.8 1.7 1.7 1.7 1.5 1.6 1.7 1.4 1.7 1.8 2.1 2.2 2.0 2.1 2.3
Intensity Change (Log2) -0.07 -0.12 -0.39 -0.44 -0.47 -0.53 -0.7 -0.68 -0.58 -0.04 0.03 0.05 -0.13 -0.09 0.02 0.13 0.22


Localization RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3
Cortical Patches 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Bud 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Bud Neck 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Bud Site 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Cell Periphery 0 -0.3 -1.3 -0.8 -1.5 -0.6 -1.0 -0.1 0.3 0 1.5 1.1 0 0
Cytoplasm 1.9 1.4 3.9 3.3 3.0 3.2 4.1 4.2 4.9 -0.5 2.4 4.9 0 0
Endoplasmic Reticulum -4.7 -5.0 -5.2 -10.0 -12.7 -10.5 -13.5 -12.4 -14.5 0.5 -2.3 -5.0 -10.0 -10.6
Endosome 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Golgi 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Mitochondria 0 0 4.9 5.6 6.7 9.0 8.9 10.3 8.4 0 0 0 0 0
Nucleus 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Nuclear Periphery 0 -1.5 -0.7 0.1 0.6 2.0 -1.0 2.1 0.4 -0.6 -2.2 -3.3 0 0
Nucleolus 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Peroxisomes 0 0 0 0 0 0 0 0 0 0 0 0 0 0
SpindlePole 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Vacuole 3.3 4.2 1.9 7.1 8.4 4.1 6.5 4.9 7.6 0 -0.5 -0.5 0 0


Endocytosis
Temp Actin Patch (Sac6-tdTomato) Cortical Patch (Sla1-GFP) Late Endosome (Snf7-GFP) Vacuole (Vph1-GFP)
37℃
RT
Cell Cycle Omics

CYCLoPs (Djp1-GFP)
Gene / Allele Actin Patch (Sac6-tdTomato) Cortical Patch (Sla1-GFP) Late Endosome (Snf7-GFP) Vacuole (Sac6-tdTomato)
Gene Images
Gene Images
Images are not yet available
Images are not yet available