Standard name
Description Splicing protein; required for splicing of two mitochondrial group I introns (BI3 in COB and AI5beta in COX1); forms a splicing complex, containing four subunits of Mrs1p and two subunits of the BI3-encoded maturase, that binds to the BI3 RNA; MRS1 has a paralog, CCE1, that arose from the whole genome duplication

Micrographs

ensLOC DeepLoc
Localization WT1 WT2 WT3 RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3 WT1 WT2 WT3 AF100 AF140 AF180
Cortical Patches 0 0 0.13 0 0 0 0 0 0 0 0 0 0.09 0 0 0 0.07 0 0 0 0 0 0 0
Bud 0 0 0.09 0.05 0 0 0.05 0.06 0.08 0 0.08 0.06 0 0 0 0.07 0 0.07 0 0 0 0 0 0
Bud Neck 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Bud Site 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Cell Periphery 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0.09 0.09 0.1 0 0 0 0 0 0
Cytoplasm 0 0.13 0.24 0.19 0.2 0.19 0.17 0.17 0.17 0.21 0.12 0.18 0.3 0.43 0.5 0.12 0.1 0.07 0 0 0 0 0 0
Endoplasmic Reticulum 0.05 0.05 0.07 0 0 0 0 0 0 0 0 0 0.05 0 0 0.06 0 0 0 0 0 0 0 0
Endosome 0 0 0 0.06 0.19 0 0 0 0 0 0 0 0.11 0.16 0.18 0.19 0.18 0.24 0.09 0.08 0.1 0.1 0.09 0.08
Golgi 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0.08 0.18 0.06 0 0 0 0 0 0
Mitochondria 0.94 0.84 0.43 0.58 0.47 0.69 0.79 0.73 0.81 0.76 0.88 0.78 0.36 0.17 0.1 0.22 0.1 0.07 0.7 0.72 0.73 0.68 0.63 0.65
Nucleus 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0.07 0 0 0 0 0 0
Nuclear Periphery 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Nucleolus 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Peroxisomes 0 0 0 0.1 0.12 0 0 0 0 0 0 0 0 0.12 0.18 0.09 0.09 0.11 0.06 0 0 0 0.06 0.07
SpindlePole 0 0 0.14 0.19 0.17 0.11 0.11 0.17 0.13 0.08 0.08 0.1 0.09 0.08 0 0.14 0.13 0.1 0 0 0 0 0 0
Vac/Vac Membrane 0 0.09 0.05 0 0.13 0.07 0 0 0 0 0 0 0.1 0.09 0.12 0.17 0.19 0.2 0 0.06 0 0 0 0.05
ensLOC DeepLoc
Localization WT1 WT2 WT3 RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3 WT1 WT2 WT3 AF100 AF140 AF180
Cortical Patches 1 4 14 6 0 0 0 1 0 0 0 0 19 8 0 5 5 3 1 3 2 6 9 13
Bud 5 5 10 13 15 14 29 33 37 19 35 23 7 6 1 10 2 5 0 0 0 1 5 5
Bud Neck 0 0 3 4 6 4 4 10 4 6 4 7 3 3 1 0 0 0 1 0 0 2 7 4
Bud Site 0 0 0 2 2 2 7 5 6 10 6 7 0 0 0 0 2 0
Cell Periphery 0 5 2 4 8 1 2 1 3 4 1 2 7 9 6 12 6 7 0 0 0 0 1 0
Cytoplasm 11 21 26 55 91 69 94 87 76 93 53 74 66 103 163 16 7 5 3 3 3 3 10 8
Endoplasmic Reticulum 12 8 7 1 0 6 0 0 1 1 1 0 10 6 3 8 3 3 2 0 0 0 3 1
Endosome 2 3 3 17 87 10 10 6 2 4 0 3 24 38 59 27 12 17 24 14 16 19 36 29
Golgi 1 1 2 1 1 0 0 0 0 0 0 1 2 0 1 11 12 4 6 1 1 1 7 5
Mitochondria 247 134 46 166 216 253 447 374 372 340 379 326 79 40 33 30 7 5 189 132 125 128 246 250
Nucleus 2 3 1 6 2 6 11 11 6 9 8 13 1 2 0 4 1 5 0 1 0 0 0 1
Nuclear Periphery 6 1 1 0 0 2 4 0 3 1 1 2 1 0 0 0 0 1 7 1 1 0 2 1
Nucleolus 3 0 0 0 0 1 1 5 4 1 2 2 0 0 0 0 0 0 0 1 0 0 3 3
Peroxisomes 1 1 0 27 54 6 7 8 8 5 2 8 5 28 59 13 6 8 16 7 6 8 22 26
SpindlePole 8 5 15 53 78 40 61 88 59 38 35 43 20 18 5 20 9 7 5 2 1 4 12 11
Vac/Vac Membrane 5 15 5 6 61 24 25 13 7 12 14 15 21 22 40 24 13 14 5 10 5 6 14 18
Unique Cell Count 262 160 107 284 455 365 568 513 458 450 433 418 220 237 323 139 68 71 270 184 172 189 394 388
Labelled Cell Count 304 206 135 361 621 438 702 642 588 543 541 526 265 283 371 180 85 84 270 184 172 189 394 388


Mitochondria

(Huh et al. Nature 2003 425:686)
Screen WT1 WT2 WT3 RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3 AF100 AF140 AF180
Mean Cell GFP Intensity (1e-4) 3.8 4.8 5.2 4.8 5.0 4.0 3.8 4.2 3.5 3.6 3.2 3.7 5.2 5.4 5.3 6.2 7.2 6.7 4.8 5.2 5.5
Std Deviation (1e-4) 0.6 0.8 1.3 1.3 1.5 1.2 1.6 1.3 1.0 1.1 1.1 1.3 0.9 1.1 1.0 1.3 1.7 1.4 1.0 1.0 1.2
Intensity Change (Log2) -0.13 -0.05 -0.38 -0.45 -0.32 -0.59 -0.53 -0.69 -0.49 0.0 0.05 0.02 0.25 0.46 0.35 -0.12 0.0 0.06


Localization RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3
Actin
Bud 0 0 0
Bud Neck 0 0 0
Bud Site 0 0 0
Cell Periphery 0 0 0
Cyto
Endoplasmic Reticulum 0 0 0
Endosome 0 0 0
Golgi 0 0 0
Mitochondria 0 0 0
Nuclear Periphery 0 0 0
Nuc
Nucleolus 0 0 0
Peroxisomes 0 0 0
SpindlePole 0 0 0
Vac
Cortical Patches 0 0 0
Cytoplasm 0 0 0
Nucleus 0 0 0
Vacuole 3.0 3.1 3.2


Endocytosis
Temp Actin Patch (Sac6-tdTomato) Cortical Patch (Sla1-GFP) Late Endosome (Snf7-GFP) Vacuole (Vph1-GFP)
37℃
RT
Cell Cycle Omics

CYCLoPs (Mrs1-GFP)
Gene / Allele Actin Patch (Sac6-tdTomato) Cortical Patch (Sla1-GFP) Late Endosome (Snf7-GFP) Vacuole (Sac6-tdTomato)
Gene Images
Gene Images
Images are not yet available
Images are not yet available