Standard name
Description Saccharopine dehydrogenase (NAD+, L-lysine-forming); catalyzes the conversion of saccharopine to L-lysine, which is the final step in the lysine biosynthesis pathway; also has mRNA binding activity
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Micrographs

ensLOC DeepLoc
Localization WT1 WT2 WT3 RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3 WT1 WT2 WT3 AF100 AF140 AF180
Cortical Patches 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 – – – 0 0 0 0 0 0
Bud 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 – – – 0 0 0 0 0 0
Bud Neck 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 – – – 0 0 0 0 0 0
Bud Site 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 – – – – – – – – –
Cell Periphery 0 0.3 0 0 0 0 0 0 0 0 0 0 0 0 0 – – – 0 0.05 0 0 0 0
Cytoplasm 0.99 0.86 1.0 0.97 0.95 0.92 0.91 0.83 0.86 0.85 0.89 0.86 0.96 1.0 0.99 – – – 0.93 0.92 0.92 0.94 0.91 0.93
Endoplasmic Reticulum 0 0 0 0 0 0.05 0.05 0.12 0.07 0.09 0.05 0.08 0 0 0 – – – 0 0 0 0 0 0
Endosome 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 – – – 0 0 0 0 0 0
Golgi 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 – – – 0 0 0 0 0 0
Mitochondria 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 – – – 0 0 0 0 0 0
Nucleus 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 – – – 0 0 0 0 0 0
Nuclear Periphery 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 – – – 0 0 0 0 0 0
Nucleolus 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 – – – 0 0 0 0 0 0
Peroxisomes 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 – – – 0 0 0 0 0 0
SpindlePole 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 – – – 0 0 0 0 0 0
Vac/Vac Membrane 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 – – – 0 0 0 0 0 0
ensLOC DeepLoc
Localization WT1 WT2 WT3 RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3 WT1 WT2 WT3 AF100 AF140 AF180
Cortical Patches 0 0 0 0 0 0 2 1 3 0 0 2 0 0 0 – – – 1 0 1 0 2 0
Bud 1 0 0 0 0 0 3 1 4 6 1 3 0 0 0 – – – 0 0 3 2 9 6
Bud Neck 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 – – – 0 0 0 0 0 0
Bud Site 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 – – – – – – – – –
Cell Periphery 1 21 3 4 5 5 9 7 7 9 6 1 2 3 2 – – – 0 6 0 0 0 0
Cytoplasm 186 61 216 194 240 188 253 168 197 217 139 136 205 342 357 – – – 159 122 204 248 326 306
Endoplasmic Reticulum 0 0 0 4 8 10 13 24 16 22 8 12 2 1 0 – – – 0 1 1 1 3 2
Endosome 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 – – – 0 0 0 0 0 0
Golgi 0 0 1 1 0 2 0 1 2 0 0 0 0 0 1 – – – 0 0 0 0 1 0
Mitochondria 0 0 0 0 0 0 0 0 0 0 0 1 0 0 0 – – – 1 0 1 0 1 0
Nucleus 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 – – – 0 0 0 1 2 2
Nuclear Periphery 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 – – – 0 0 0 0 0 1
Nucleolus 1 0 0 0 0 2 1 2 0 0 1 1 1 0 1 – – – 0 0 0 0 0 1
Peroxisomes 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 – – – 0 0 0 0 0 0
SpindlePole 1 0 0 0 0 0 1 0 0 0 0 0 0 0 0 – – – 0 0 1 0 1 2
Vac/Vac Membrane 0 0 0 0 1 1 6 7 7 7 5 7 6 0 1 – – – 0 0 3 1 1 0
Unique Cell Count 188 71 216 200 252 204 278 202 229 254 157 159 213 343 360 171 133 223 263 357 328
Labelled Cell Count 190 82 220 203 254 208 288 211 236 261 160 163 216 346 362 171 133 223 263 357 328


Cytoplasm, Nucleus

(Huh et al. Nature 2003 425:686)
Screen WT1 WT2 WT3 RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3 AF100 AF140 AF180
Mean Cell GFP Intensity (1e-4) 420.3 521.1 198.2 179.7 114.2 104.2 84.2 87.0 74.4 74.6 62.7 60.6 150.4 162.4 148.9 – – – 168.3 165.0 165.3
Std Deviation (1e-4) 92.3 95.0 136.0 122.4 76.2 68.0 54.6 54.6 43.5 36.4 32.0 28.0 109.0 113.7 100.8 – – – 126.3 124.7 122.2
Intensity Change (Log2) – – – -0.14 -0.8 -0.93 -1.23 -1.19 -1.41 -1.41 -1.66 -1.71 -0.4 -0.29 -0.41 – – – -0.24 -0.27 -0.26


Localization RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3
Cortical Patches 0 0 0 0 0 0 0 0 0 0 0 0 – – –
Bud 0 0 0 0 0 0 0 0 0 0 0 0 – – –
Bud Neck 0 0 0 0 0 0 0 0 0 0 0 0 – – –
Bud Site 0 0 0 0 0 0 0 0 0 0 0 0 – – –
Cell Periphery 0 0 0 1.3 0 0 1.5 0 0 0 0 0 – – –
Cytoplasm -2.6 -3.2 -4.2 -4.5 -6.3 -5.7 -5.8 -5.1 -5.8 -2.9 -0.8 -1.3 – – –
Endoplasmic Reticulum 0 0 0 3.2 5.2 4.0 4.4 0 4.1 0 0 0 – – –
Endosome 0 0 0 0 0 0 0 0 0 0 0 0 – – –
Golgi 0 0 0 0 0 0 0 0 0 0 0 0 – – –
Mitochondria 0 0 0 0 0 0 0 0 0 0 0 0 – – –
Nucleus 0 0 0 0 0 0 0 0 0 0 0 0 – – –
Nuclear Periphery 0 0 0 0 0 0 0 0 0 0 0 0 – – –
Nucleolus 0 0 0 0 0 0 0 0 0 0 0 0 – – –
Peroxisomes 0 0 0 0 0 0 0 0 0 0 0 0 – – –
SpindlePole 0 0 0 0 0 0 0 0 0 0 0 0 – – –
Vacuole 0 0 0 0 0 0 0 0 0 0 0 0 – – –

Images

Circle plot not available

Protein Concentration and Protein Localization Data

R1 R2 R3
G1 Pre-START G1 Post-START S/G2 Metaphase Anaphase Telophase G1 Pre-START G1 Post-START S/G2 Metaphase Anaphase Telophase G1 Pre-START G1 Post-START S/G2 Metaphase Anaphase Telophase
Concentration – – – – – – – – – – – – – – – – – –
Actin 0.0033 0.0002 0.0012 0.0002 0.0004 0.0002 0.0053 0.0042 0.0054 0.0006 0.0075 0.0003 0.0009 0 0.0001 0.0027 0.0002 0.0001
Bud 0.0045 0.0001 0.0005 0.0001 0.0001 0.0012 0.0003 0.0066 0.0048 0.0009 0.0213 0.0002 0.0179 0 0.0001 0.0036 0.0001 0.0001
Bud Neck 0.0003 0.0001 0.0006 0.0002 0.0002 0.0004 0.0001 0.0001 0.0002 0.0002 0.0015 0.0004 0.0001 0 0.0001 0.0002 0.0001 0.0003
Bud Periphery 0.002 0 0.0002 0 0 0.0002 0.0002 0.0013 0.001 0.0002 0.0052 0 0.0054 0 0 0.001 0 0
Bud Site 0.0024 0 0.0009 0.0001 0.0006 0.0005 0.0002 0.0015 0.0011 0.0001 0.0011 0 0.0018 0 0 0.0003 0 0
Cell Periphery 0.0009 0 0.0003 0.0004 0.0002 0.0009 0.0002 0.0004 0.0004 0.0001 0.0003 0.0001 0.0018 0 0 0.0009 0 0
Cytoplasm 0.8695 0.9874 0.933 0.9085 0.7958 0.6892 0.8705 0.9716 0.963 0.9733 0.4699 0.987 0.9265 0.9979 0.9943 0.8842 0.9962 0.9887
Cytoplasmic Foci 0.0109 0.0004 0.013 0.0285 0.025 0.0514 0.0002 0.003 0.0031 0.0011 0.0182 0.0008 0.0006 0 0 0.0012 0.0001 0.0001
Eisosomes 0.0004 0 0.0003 0.0004 0.0004 0.0009 0 0 0 0 0 0 0 0 0 0.0001 0 0
Endoplasmic Reticulum 0.0023 0 0.0023 0.0002 0.0005 0.0001 0.0161 0.0001 0.0004 0.0002 0.0075 0.0001 0.0002 0 0 0.0004 0 0
Endosome 0.0065 0 0.0067 0.0001 0.0004 0.0001 0.0016 0.0003 0.0014 0.0001 0.2295 0 0.0001 0 0 0.0013 0 0
Golgi 0.0008 0 0.0006 0.0001 0.0003 0.0001 0.0003 0.0001 0.0002 0 0.0714 0 0 0 0 0.0004 0 0
Lipid Particles 0.0013 0 0.0012 0.0054 0.0033 0.0028 0.0001 0.0002 0.0002 0 0.0003 0 0.0001 0 0 0.0001 0 0
Mitochondria 0.0099 0.0003 0.0067 0.004 0.0018 0.0346 0.0008 0.0012 0.0006 0.0003 0.1233 0.0001 0.0085 0 0.0001 0.0163 0.0002 0.0002
None 0.0119 0.0024 0.0094 0.0158 0.0778 0.0812 0.0013 0.0022 0.0034 0.0014 0.0017 0.0008 0.0028 0.0001 0.0001 0.004 0.0001 0.0002
Nuclear Periphery 0.0212 0.0013 0.0024 0.0036 0.0028 0.0005 0.0494 0.0009 0.0021 0.0024 0.0033 0.0011 0.0023 0.0002 0.0004 0.0247 0.0002 0.0006
Nucleolus 0.0103 0 0.0085 0.0096 0.0287 0.0966 0.0003 0.0004 0.0007 0.0001 0.0002 0.0001 0.0028 0 0 0.0025 0 0
Nucleus 0.0332 0.0074 0.0073 0.0167 0.0161 0.032 0.049 0.0037 0.0057 0.0178 0.0161 0.0077 0.0131 0.0017 0.0047 0.0271 0.0029 0.0094
Peroxisomes 0.0006 0 0.0004 0.0005 0.0004 0.0019 0 0.0002 0.0001 0 0.0007 0 0.0003 0 0 0.0003 0 0
Punctate Nuclear 0.0017 0.0001 0.0003 0.0011 0.0443 0.0018 0.0004 0.0004 0.0007 0.0004 0.0004 0.0004 0.0001 0 0 0.0003 0 0.0001
Vacuole 0.0033 0 0.0029 0.0018 0.0006 0.0019 0.0018 0.0013 0.0043 0.0006 0.0089 0.0007 0.0078 0 0.0002 0.0075 0 0
Vacuole Periphery 0.003 0 0.0013 0.0027 0.0005 0.0015 0.002 0.0005 0.0012 0.0002 0.0116 0.0001 0.0067 0 0 0.0209 0 0

Sequencing Data

R1 R2
G1 Post-START S/G2 Metaphase Anaphase Telophase G1 Post-START S/G2 Metaphase Anaphase Telophase
Gene Expression 171.6079 171.429 120.6843 124.3999 131.9682 253.5836 235.7756 203.0778 178.032 160.6683
Translational Efficiency 1.9963 1.7701 1.7172 1.6652 1.9818 2.3976 1.7954 1.8586 1.7586 1.77

Hit Data

Dataset Hit
Protein Abundance –
Protein Localization ✘
Gene Expression ✔
Translational Efficiency ✘