Standard name | |
---|---|
Description | Putative benzil reductase;(GFP)-fusion protein localizes to the cytoplasm and is induced by the DNA-damaging agent MMS; sequence similarity with short-chain dehydrogenase/reductases; null mutant has increased spontaneous Rad52p foci |
ensLOC | DeepLoc | |||||||||||||||||||||||
---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|
Localization | WT1 | WT2 | WT3 | RAP60 | RAP140 | RAP220 | RAP300 | RAP380 | RAP460 | RAP540 | RAP620 | RAP700 | HU80 | HU120 | HU160 | rpd3Δ_1 | rpd3Δ_2 | rpd3Δ_3 | WT1 | WT2 | WT3 | AF100 | AF140 | AF180 |
Cortical Patches | 0 | 0 | 0 | 0 | 0 | 0 | 0 | – | 0 | 1 | 1 | 1 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 2 | 0 | 3 | 5 |
Bud | 3 | 1 | 0 | 0 | 0 | 1 | 0 | – | 2 | 1 | 0 | 0 | 0 | 0 | 1 | 0 | 0 | 0 | 0 | 1 | 0 | 1 | 16 | 24 |
Bud Neck | 0 | 0 | 1 | 0 | 0 | 0 | 0 | – | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 1 | 0 | 0 | 1 | 2 |
Bud Site | 0 | 0 | 0 | 0 | 0 | 0 | 0 | – | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | – | – | – | – | – | – |
Cell Periphery | 0 | 0 | 2 | 1 | 0 | 4 | 1 | – | 4 | 2 | 4 | 1 | 1 | 1 | 2 | 8 | 8 | 7 | 0 | 0 | 0 | 0 | 0 | 1 |
Cytoplasm | 116 | 211 | 202 | 152 | 231 | 179 | 179 | – | 146 | 204 | 92 | 135 | 430 | 589 | 704 | 589 | 387 | 431 | 130 | 214 | 194 | 354 | 492 | 707 |
Endoplasmic Reticulum | 0 | 0 | 2 | 0 | 1 | 1 | 0 | – | 0 | 5 | 4 | 0 | 3 | 2 | 2 | 26 | 13 | 15 | 0 | 1 | 0 | 1 | 6 | 5 |
Endosome | 0 | 0 | 2 | 0 | 0 | 0 | 1 | – | 1 | 0 | 1 | 1 | 0 | 2 | 0 | 2 | 0 | 1 | 0 | 1 | 1 | 3 | 3 | 4 |
Golgi | 0 | 0 | 1 | 0 | 0 | 0 | 0 | – | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 2 | 1 | 1 | 1 | 1 | 0 | 0 | 0 |
Mitochondria | 1 | 10 | 0 | 1 | 0 | 9 | 17 | – | 35 | 49 | 36 | 56 | 0 | 0 | 0 | 6 | 3 | 4 | 0 | 6 | 1 | 1 | 5 | 3 |
Nucleus | 2 | 1 | 1 | 0 | 1 | 0 | 7 | – | 4 | 17 | 3 | 10 | 2 | 3 | 1 | 12 | 19 | 16 | 0 | 1 | 0 | 4 | 0 | 4 |
Nuclear Periphery | 1 | 1 | 0 | 0 | 0 | 0 | 0 | – | 2 | 0 | 0 | 5 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 1 | 2 |
Nucleolus | 1 | 1 | 0 | 0 | 0 | 0 | 0 | – | 0 | 0 | 0 | 0 | 0 | 1 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 1 |
Peroxisomes | 0 | 0 | 0 | 0 | 0 | 0 | 0 | – | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
SpindlePole | 0 | 0 | 0 | 0 | 1 | 0 | 0 | – | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 1 | 2 | 1 | 5 | 5 | 11 |
Vac/Vac Membrane | 0 | 0 | 2 | 1 | 7 | 18 | 29 | – | 44 | 47 | 30 | 43 | 2 | 2 | 11 | 21 | 13 | 37 | 4 | 2 | 0 | 2 | 1 | 6 |
Unique Cell Count | 121 | 219 | 208 | 152 | 240 | 201 | 222 | 214 | 292 | 150 | 214 | 436 | 595 | 710 | 618 | 408 | 470 | 142 | 236 | 207 | 381 | 546 | 791 | |
Labelled Cell Count | 124 | 225 | 213 | 155 | 241 | 212 | 234 | 238 | 326 | 171 | 252 | 438 | 600 | 721 | 664 | 445 | 512 | 142 | 236 | 207 | 381 | 546 | 791 |
Screen | WT1 | WT2 | WT3 | RAP60 | RAP140 | RAP220 | RAP300 | RAP380 | RAP460 | RAP540 | RAP620 | RAP700 | HU80 | HU120 | HU160 | rpd3Δ_1 | rpd3Δ_2 | rpd3Δ_3 | AF100 | AF140 | AF180 |
---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|
Mean Cell GFP Intensity (1e-4) | 5.0 | 5.1 | 6.8 | 6.1 | 7.4 | 7.3 | 7.3 | – | 7.8 | 7.6 | 7.2 | 7.8 | 6.9 | 7.3 | 7.2 | 9.8 | 12.2 | 13.2 | 7.4 | 8.0 | 8.0 |
Std Deviation (1e-4) | 0.8 | 1.5 | 1.2 | 0.9 | 1.4 | 1.7 | 1.5 | – | 2.3 | 2.0 | 2.3 | 2.5 | 1.3 | 1.3 | 1.3 | 2.1 | 2.2 | 2.5 | 2.0 | 2.3 | 2.3 |
Intensity Change (Log2) | – | – | – | -0.17 | 0.13 | 0.1 | 0.1 | – | 0.21 | 0.16 | 0.09 | 0.19 | 0.03 | 0.1 | 0.08 | 0.53 | 0.84 | 0.96 | 0.13 | 0.24 | 0.23 |
Localization | RAP60 | RAP140 | RAP220 | RAP300 | RAP380 | RAP460 | RAP540 | RAP620 | RAP700 | HU80 | HU120 | HU160 | rpd3Δ_1 | rpd3Δ_2 | rpd3Δ_3 |
---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|
Cortical Patches | 0 | 0 | 0 | 0 | – | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
Bud | 0 | 0 | 0 | 0 | – | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
Bud Neck | 0 | 0 | 0 | 0 | – | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
Bud Site | 0 | 0 | 0 | 0 | – | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
Cell Periphery | 0 | 0 | 0 | 0 | – | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
Cytoplasm | 2.1 | -0.5 | -3.2 | -5.4 | – | -7.8 | -7.7 | -8.7 | -8.7 | 1.3 | 1.9 | 2.3 | -1.1 | -1.3 | -2.6 |
Endoplasmic Reticulum | 0 | 0 | 0 | 0 | – | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 2.2 | 1.7 | 1.7 |
Endosome | 0 | 0 | 0 | 0 | – | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
Golgi | 0 | 0 | 0 | 0 | – | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
Mitochondria | 0 | 0 | 0 | 4.1 | – | 6.1 | 6.2 | 7.4 | 7.9 | 0 | 0 | 0 | 0 | 0 | 0 |
Nucleus | 0 | 0 | 0 | 0 | – | 0 | 3.2 | 0 | 2.7 | 0 | 0 | 0 | 0 | 2.8 | 2.2 |
Nuclear Periphery | 0 | 0 | 0 | 0 | – | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
Nucleolus | 0 | 0 | 0 | 0 | – | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
Peroxisomes | 0 | 0 | 0 | 0 | – | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
SpindlePole | 0 | 0 | 0 | 0 | – | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
Vacuole | 0 | 0 | 3.7 | 4.8 | – | 6.5 | 5.6 | 6.2 | 6.4 | 0 | 0 | 0 | 1.8 | 1.7 | 3.6 |