Standard name
Description Putative benzil reductase;(GFP)-fusion protein localizes to the cytoplasm and is induced by the DNA-damaging agent MMS; sequence similarity with short-chain dehydrogenase/reductases; null mutant has increased spontaneous Rad52p foci
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Micrographs

ensLOC DeepLoc
Localization WT1 WT2 WT3 RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3 WT1 WT2 WT3 AF100 AF140 AF180
Cortical Patches 0 0 0 0 0 0 0 – 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Bud 0 0 0 0 0 0 0 – 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Bud Neck 0 0 0 0 0 0 0 – 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Bud Site 0 0 0 0 0 0 0 – 0 0 0 0 0 0 0 0 0 0 – – – – – –
Cell Periphery 0 0 0 0 0 0 0 – 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Cytoplasm 0.96 0.96 0.97 1.0 0.96 0.89 0.81 – 0.68 0.7 0.61 0.63 0.99 0.99 0.99 0.95 0.95 0.92 0.92 0.91 0.94 0.93 0.9 0.89
Endoplasmic Reticulum 0 0 0 0 0 0 0 – 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Endosome 0 0 0 0 0 0 0 – 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Golgi 0 0 0 0 0 0 0 – 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Mitochondria 0 0.05 0 0 0 0 0.08 – 0.16 0.17 0.24 0.26 0 0 0 0 0 0 0 0 0 0 0 0
Nucleus 0 0 0 0 0 0 0 – 0 0.06 0 0.05 0 0 0 0 0.05 0 0 0 0 0 0 0
Nuclear Periphery 0 0 0 0 0 0 0 – 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Nucleolus 0 0 0 0 0 0 0 – 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Peroxisomes 0 0 0 0 0 0 0 – 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
SpindlePole 0 0 0 0 0 0 0 – 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Vac/Vac Membrane 0 0 0 0 0 0.09 0.13 – 0.21 0.16 0.2 0.2 0 0 0 0 0 0.08 0 0 0 0 0 0
ensLOC DeepLoc
Localization WT1 WT2 WT3 RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3 WT1 WT2 WT3 AF100 AF140 AF180
Cortical Patches 0 0 0 0 0 0 0 – 0 1 1 1 0 0 0 0 0 0 0 0 2 0 3 5
Bud 3 1 0 0 0 1 0 – 2 1 0 0 0 0 1 0 0 0 0 1 0 1 16 24
Bud Neck 0 0 1 0 0 0 0 – 0 0 0 0 0 0 0 0 0 0 0 1 0 0 1 2
Bud Site 0 0 0 0 0 0 0 – 0 0 0 0 0 0 0 0 0 0 – – – – – –
Cell Periphery 0 0 2 1 0 4 1 – 4 2 4 1 1 1 2 8 8 7 0 0 0 0 0 1
Cytoplasm 116 211 202 152 231 179 179 – 146 204 92 135 430 589 704 589 387 431 130 214 194 354 492 707
Endoplasmic Reticulum 0 0 2 0 1 1 0 – 0 5 4 0 3 2 2 26 13 15 0 1 0 1 6 5
Endosome 0 0 2 0 0 0 1 – 1 0 1 1 0 2 0 2 0 1 0 1 1 3 3 4
Golgi 0 0 1 0 0 0 0 – 0 0 0 0 0 0 0 0 2 1 1 1 1 0 0 0
Mitochondria 1 10 0 1 0 9 17 – 35 49 36 56 0 0 0 6 3 4 0 6 1 1 5 3
Nucleus 2 1 1 0 1 0 7 – 4 17 3 10 2 3 1 12 19 16 0 1 0 4 0 4
Nuclear Periphery 1 1 0 0 0 0 0 – 2 0 0 5 0 0 0 0 0 0 0 0 0 0 1 2
Nucleolus 1 1 0 0 0 0 0 – 0 0 0 0 0 1 0 0 0 0 0 0 0 0 0 1
Peroxisomes 0 0 0 0 0 0 0 – 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
SpindlePole 0 0 0 0 1 0 0 – 0 0 0 0 0 0 0 0 0 0 1 2 1 5 5 11
Vac/Vac Membrane 0 0 2 1 7 18 29 – 44 47 30 43 2 2 11 21 13 37 4 2 0 2 1 6
Unique Cell Count 121 219 208 152 240 201 222 214 292 150 214 436 595 710 618 408 470 142 236 207 381 546 791
Labelled Cell Count 124 225 213 155 241 212 234 238 326 171 252 438 600 721 664 445 512 142 236 207 381 546 791


Cytoplasm

(Huh et al. Nature 2003 425:686)
Screen WT1 WT2 WT3 RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3 AF100 AF140 AF180
Mean Cell GFP Intensity (1e-4) 5.0 5.1 6.8 6.1 7.4 7.3 7.3 – 7.8 7.6 7.2 7.8 6.9 7.3 7.2 9.8 12.2 13.2 7.4 8.0 8.0
Std Deviation (1e-4) 0.8 1.5 1.2 0.9 1.4 1.7 1.5 – 2.3 2.0 2.3 2.5 1.3 1.3 1.3 2.1 2.2 2.5 2.0 2.3 2.3
Intensity Change (Log2) – – – -0.17 0.13 0.1 0.1 – 0.21 0.16 0.09 0.19 0.03 0.1 0.08 0.53 0.84 0.96 0.13 0.24 0.23


Localization RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3
Cortical Patches 0 0 0 0 – 0 0 0 0 0 0 0 0 0 0
Bud 0 0 0 0 – 0 0 0 0 0 0 0 0 0 0
Bud Neck 0 0 0 0 – 0 0 0 0 0 0 0 0 0 0
Bud Site 0 0 0 0 – 0 0 0 0 0 0 0 0 0 0
Cell Periphery 0 0 0 0 – 0 0 0 0 0 0 0 0 0 0
Cytoplasm 2.1 -0.5 -3.2 -5.4 – -7.8 -7.7 -8.7 -8.7 1.3 1.9 2.3 -1.1 -1.3 -2.6
Endoplasmic Reticulum 0 0 0 0 – 0 0 0 0 0 0 0 2.2 1.7 1.7
Endosome 0 0 0 0 – 0 0 0 0 0 0 0 0 0 0
Golgi 0 0 0 0 – 0 0 0 0 0 0 0 0 0 0
Mitochondria 0 0 0 4.1 – 6.1 6.2 7.4 7.9 0 0 0 0 0 0
Nucleus 0 0 0 0 – 0 3.2 0 2.7 0 0 0 0 2.8 2.2
Nuclear Periphery 0 0 0 0 – 0 0 0 0 0 0 0 0 0 0
Nucleolus 0 0 0 0 – 0 0 0 0 0 0 0 0 0 0
Peroxisomes 0 0 0 0 – 0 0 0 0 0 0 0 0 0 0
SpindlePole 0 0 0 0 – 0 0 0 0 0 0 0 0 0 0
Vacuole 0 0 3.7 4.8 – 6.5 5.6 6.2 6.4 0 0 0 1.8 1.7 3.6