Standard name
Human Ortholog
Description Putative benzil reductase;(GFP)-fusion protein localizes to the cytoplasm and is induced by the DNA-damaging agent MMS; sequence similarity with short-chain dehydrogenase/reductases; null mutant has increased spontaneous Rad52p foci

Micrographs

ensLOC DeepLoc
Localization WT1 WT2 WT3 RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3 WT1 WT2 WT3 AF100 AF140 AF180
Cortical Patches 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Bud 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Bud Neck 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Bud Site 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Cell Periphery 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Cytoplasm 0.96 0.96 0.97 1.0 0.96 0.89 0.81 0.68 0.7 0.61 0.63 0.99 0.99 0.99 0.95 0.95 0.92 0.92 0.91 0.94 0.93 0.9 0.89
Endoplasmic Reticulum 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Endosome 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Golgi 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Mitochondria 0 0.05 0 0 0 0 0.08 0.16 0.17 0.24 0.26 0 0 0 0 0 0 0 0 0 0 0 0
Nucleus 0 0 0 0 0 0 0 0 0.06 0 0.05 0 0 0 0 0.05 0 0 0 0 0 0 0
Nuclear Periphery 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Nucleolus 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Peroxisomes 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
SpindlePole 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Vac/Vac Membrane 0 0 0 0 0 0.09 0.13 0.21 0.16 0.2 0.2 0 0 0 0 0 0.08 0 0 0 0 0 0
ensLOC DeepLoc
Localization WT1 WT2 WT3 RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3 WT1 WT2 WT3 AF100 AF140 AF180
Cortical Patches 0 0 0 0 0 0 0 0 1 1 1 0 0 0 0 0 0 0 0 2 0 3 5
Bud 3 1 0 0 0 1 0 2 1 0 0 0 0 1 0 0 0 0 1 0 1 16 24
Bud Neck 0 0 1 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 1 0 0 1 2
Bud Site 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Cell Periphery 0 0 2 1 0 4 1 4 2 4 1 1 1 2 8 8 7 0 0 0 0 0 1
Cytoplasm 116 211 202 152 231 179 179 146 204 92 135 430 589 704 589 387 431 130 214 194 354 492 707
Endoplasmic Reticulum 0 0 2 0 1 1 0 0 5 4 0 3 2 2 26 13 15 0 1 0 1 6 5
Endosome 0 0 2 0 0 0 1 1 0 1 1 0 2 0 2 0 1 0 1 1 3 3 4
Golgi 0 0 1 0 0 0 0 0 0 0 0 0 0 0 0 2 1 1 1 1 0 0 0
Mitochondria 1 10 0 1 0 9 17 35 49 36 56 0 0 0 6 3 4 0 6 1 1 5 3
Nucleus 2 1 1 0 1 0 7 4 17 3 10 2 3 1 12 19 16 0 1 0 4 0 4
Nuclear Periphery 1 1 0 0 0 0 0 2 0 0 5 0 0 0 0 0 0 0 0 0 0 1 2
Nucleolus 1 1 0 0 0 0 0 0 0 0 0 0 1 0 0 0 0 0 0 0 0 0 1
Peroxisomes 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
SpindlePole 0 0 0 0 1 0 0 0 0 0 0 0 0 0 0 0 0 1 2 1 5 5 11
Vac/Vac Membrane 0 0 2 1 7 18 29 44 47 30 43 2 2 11 21 13 37 4 2 0 2 1 6
Unique Cell Count 121 219 208 152 240 201 222 214 292 150 214 436 595 710 618 408 470 142 236 207 381 546 791
Labelled Cell Count 124 225 213 155 241 212 234 238 326 171 252 438 600 721 664 445 512 142 236 207 381 546 791


Cytoplasm

(Huh et al. Nature 2003 425:686)
Screen WT1 WT2 WT3 RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3 AF100 AF140 AF180
Mean Cell GFP Intensity (1e-4) 5.0 5.1 6.8 6.1 7.4 7.3 7.3 7.8 7.6 7.2 7.8 6.9 7.3 7.2 9.8 12.2 13.2 7.4 8.0 8.0
Std Deviation (1e-4) 0.8 1.5 1.2 0.9 1.4 1.7 1.5 2.3 2.0 2.3 2.5 1.3 1.3 1.3 2.1 2.2 2.5 2.0 2.3 2.3
Intensity Change (Log2) -0.17 0.13 0.1 0.1 0.21 0.16 0.09 0.19 0.03 0.1 0.08 0.53 0.84 0.96 0.13 0.24 0.23


Localization RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3
Cortical Patches 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Bud 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Bud Neck 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Bud Site 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Cell Periphery 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Cytoplasm 2.1 -0.5 -3.2 -5.4 -7.8 -7.7 -8.7 -8.7 1.3 1.9 2.3 -1.1 -1.3 -2.6
Endoplasmic Reticulum 0 0 0 0 0 0 0 0 0 0 0 2.2 1.7 1.7
Endosome 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Golgi 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Mitochondria 0 0 0 4.1 6.1 6.2 7.4 7.9 0 0 0 0 0 0
Nucleus 0 0 0 0 0 3.2 0 2.7 0 0 0 0 2.8 2.2
Nuclear Periphery 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Nucleolus 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Peroxisomes 0 0 0 0 0 0 0 0 0 0 0 0 0 0
SpindlePole 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Vacuole 0 0 3.7 4.8 6.5 5.6 6.2 6.4 0 0 0 1.8 1.7 3.6

Images

Circle plot not available

Protein Concentration and Protein Localization Data

R1 R2 R3
G1 Pre-START G1 Post-START S/G2 Metaphase Anaphase Telophase G1 Pre-START G1 Post-START S/G2 Metaphase Anaphase Telophase G1 Pre-START G1 Post-START S/G2 Metaphase Anaphase Telophase
Concentration 2.1788 2.3015 2.041 1.9573 2.5564 2.1829 5.3604 5.5845 5.3931 4.6624 5.1323 5.2056 3.1643 3.2048 3.0946 2.9889 2.6353 3.2057
Actin 0.0248 0.0008 0.0184 0.0006 0.0426 0.0009 0.0466 0.0025 0.0056 0.0011 0.0409 0.0091 0.0651 0.0105 0.0044 0.0176 0.0059 0.0051
Bud 0.0003 0.0021 0.0002 0.0002 0.0023 0.0001 0.001 0.0005 0.0006 0.0005 0.0011 0.0004 0.0005 0.0014 0.0004 0.0004 0.0013 0.0001
Bud Neck 0.005 0.0003 0.0005 0.0003 0.0121 0.0007 0.0054 0.0002 0.0028 0.0008 0.0013 0.0013 0.0083 0.0005 0.003 0.0008 0.0023 0.0013
Bud Periphery 0.0004 0.0015 0.0002 0.0001 0.0039 0.0001 0.0024 0.0002 0.0004 0.0004 0.0015 0.0003 0.0006 0.001 0.0004 0.0003 0.0014 0.0001
Bud Site 0.0015 0.0047 0.0005 0.0002 0.0095 0.0001 0.0029 0.0021 0.004 0.0002 0.0005 0.0004 0.0014 0.0121 0.0018 0.0005 0.0074 0.0001
Cell Periphery 0.0003 0.0002 0.0001 0 0.0007 0 0.0002 0.0001 0.0002 0.0001 0.0001 0.0001 0.0002 0.0003 0.0001 0.0001 0.0002 0
Cytoplasm 0.6894 0.7571 0.8026 0.7494 0.8558 0.8634 0.5228 0.7581 0.7392 0.7219 0.6672 0.7738 0.7042 0.746 0.8242 0.7853 0.7982 0.8627
Cytoplasmic Foci 0.0264 0.006 0.0093 0.0196 0.0139 0.0089 0.0217 0.0075 0.0134 0.0069 0.0119 0.0129 0.0146 0.0067 0.0065 0.0112 0.0119 0.005
Eisosomes 0.0003 0.0001 0.0002 0 0.0002 0 0.0002 0.0001 0.0001 0 0.0002 0.0001 0.0004 0.0001 0 0.0001 0.0001 0
Endoplasmic Reticulum 0.0054 0.004 0.0047 0.0048 0.0042 0.0029 0.0069 0.0027 0.0023 0.0028 0.0027 0.0021 0.003 0.0033 0.0036 0.0049 0.003 0.0023
Endosome 0.0149 0.0042 0.0079 0.0057 0.0109 0.0048 0.0199 0.0033 0.0051 0.0021 0.0129 0.0071 0.0207 0.0024 0.002 0.0135 0.0141 0.0025
Golgi 0.0048 0.0003 0.0016 0.0009 0.0028 0.0002 0.0085 0.0004 0.0042 0.0002 0.0057 0.0012 0.005 0.0011 0.0003 0.0018 0.0023 0.001
Lipid Particles 0.0032 0.0003 0.0015 0.0118 0.0003 0.0001 0.0057 0.0002 0.0017 0.0001 0.0164 0.0022 0.008 0.001 0.0001 0.0009 0.0008 0.0004
Mitochondria 0.001 0.0004 0.0013 0.0005 0.0022 0.0003 0.002 0.0002 0.0015 0.0003 0.0047 0.0031 0.0019 0.0005 0.0003 0.0014 0.0014 0.0003
None 0.1843 0.2055 0.1303 0.1533 0.0254 0.0977 0.2869 0.2133 0.1942 0.2566 0.1715 0.1704 0.1215 0.1938 0.1385 0.1351 0.1219 0.1064
Nuclear Periphery 0.0048 0.0023 0.0032 0.0031 0.0043 0.0084 0.0124 0.0015 0.0037 0.0012 0.0104 0.0011 0.0104 0.0014 0.0022 0.0097 0.0031 0.0019
Nucleolus 0.0013 0.0002 0.0002 0.0002 0.0004 0.0001 0.0013 0.0001 0.0005 0.0001 0.0012 0.0001 0.0008 0.0004 0.0002 0.0003 0.0015 0.0001
Nucleus 0.0136 0.0063 0.0119 0.0068 0.0044 0.0086 0.0343 0.0033 0.0061 0.0029 0.0058 0.0051 0.0158 0.0113 0.0079 0.0072 0.0129 0.009
Peroxisomes 0.0055 0.0001 0.0012 0.0371 0.0011 0.0001 0.0034 0.0006 0.0105 0.0002 0.0195 0.0066 0.0068 0.0008 0.0003 0.0012 0.001 0.0001
Punctate Nuclear 0.0105 0.0015 0.0019 0.0033 0.001 0.001 0.0133 0.0023 0.0025 0.001 0.0228 0.0014 0.0077 0.0035 0.0026 0.0059 0.0051 0.001
Vacuole 0.0018 0.0019 0.0017 0.0018 0.0013 0.0013 0.0016 0.0008 0.0013 0.0007 0.0009 0.0011 0.0022 0.0016 0.0009 0.0012 0.0037 0.0003
Vacuole Periphery 0.0005 0.0003 0.0004 0.0003 0.0004 0.0003 0.0006 0.0001 0.0002 0.0001 0.0009 0.0002 0.0009 0.0002 0.0001 0.0006 0.0006 0.0001

Sequencing Data

R1 R2
G1 Post-START S/G2 Metaphase Anaphase Telophase G1 Post-START S/G2 Metaphase Anaphase Telophase
Gene Expression 39.0092 32.381 35.118 30.9785 44.255 40.3801 34.3145 31.4466 47.47 35.1827
Translational Efficiency 0.9902 0.9461 1.0446 1.8543 1.1821 1.241 0.9885 1.5864 1.1878 1.2103

Hit Data

Dataset Hit
Protein Concentration
Protein Localization
Gene Expression
Translational Efficiency
Standard name
Human Ortholog
Description Putative benzil reductase;(GFP)-fusion protein localizes to the cytoplasm and is induced by the DNA-damaging agent MMS; sequence similarity with short-chain dehydrogenase/reductases; null mutant has increased spontaneous Rad52p foci
Localization
Cell Percentages cytoplasm (84%)
Cell Cycle Regulation No
Subcompartmental Group cytoplasm-9

Irc24

Irc24


Nearest neighbours ( genes)

correlation threshold

0.6

1.0

0.80


Biological Process (Gene Ontology)

Molecular Function (Gene Ontology)

Cellular Component (Gene Ontology)


Endocytosis
Temp Actin Patch (Sac6-tdTomato) Cortical Patch (Sla1-GFP) Late Endosome (Snf7-GFP) Vacuole (Vph1-GFP)
37℃
RT
Cell Cycle Omics

CYCLoPs (Irc24-GFP)
Gene / Allele Actin Patch (Sac6-tdTomato) Cortical Patch (Sla1-GFP) Late Endosome (Snf7-GFP) Vacuole (Sac6-tdTomato)
Gene Images
Gene Images
Images are not yet available
Images are not yet available