Standard name
Description Essential conserved subunit of CPF cleavage and polyadenylation factor; plays a role in 3' end formation of mRNA via the specific cleavage and polyadenylation of pre-mRNA; contains a ubiquitin-like (UBL) domain, a RNA-binding zinc knuckle motif and a RING finger domain; both the zinc knuckle and RING finger are required for pre-mRNA binding; possible role in ubiquitination of Pap1p; relocalizes to the cytosol in response to hypoxia
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Micrographs

ensLOC DeepLoc
Localization WT1 WT2 WT3 RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3 WT1 WT2 WT3 AF100 AF140 AF180
Cortical Patches 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Bud 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Bud Neck 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Bud Site 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 – – – – – –
Cell Periphery 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Cytoplasm 0 0 0 0 0.05 0 0 0 0 0 0 0 0.09 0.11 0.15 0.09 0.1 0.08 0 0 0 0 0 0
Endoplasmic Reticulum 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Endosome 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Golgi 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Mitochondria 0.14 0.08 0 0.08 0.05 0.17 0.17 0.15 0.18 0.25 0.25 0.23 0 0 0 0 0 0 0 0 0 0 0 0
Nucleus 0.92 0.93 0.95 0.92 0.9 0.86 0.85 0.83 0.85 0.8 0.83 0.81 0.91 0.87 0.83 0.85 0.83 0.84 0.89 0.94 0.9 0.94 0.88 0.86
Nuclear Periphery 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Nucleolus 0.22 0.09 0.14 0.24 0.09 0.17 0.23 0.25 0.27 0.24 0.33 0.3 0 0 0 0.07 0 0 0.06 0 0.05 0 0 0
Peroxisomes 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
SpindlePole 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Vac/Vac Membrane 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0.05
ensLOC DeepLoc
Localization WT1 WT2 WT3 RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3 WT1 WT2 WT3 AF100 AF140 AF180
Cortical Patches 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Bud 0 0 0 0 2 1 0 4 1 0 2 2 0 1 0 0 0 0 1 0 1 0 0 1
Bud Neck 0 0 0 0 0 0 0 2 2 0 0 1 0 0 0 0 0 0 0 0 0 0 0 0
Bud Site 0 0 0 0 1 0 4 3 6 5 1 3 0 1 1 0 0 0 – – – – – –
Cell Periphery 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Cytoplasm 3 3 1 1 11 6 8 11 3 5 1 1 12 35 35 18 18 10 0 0 0 0 1 3
Endoplasmic Reticulum 0 0 0 0 0 0 0 0 0 0 0 0 1 0 0 3 3 3 0 0 0 0 1 0
Endosome 0 1 0 0 0 0 0 0 0 0 0 0 0 0 0 1 1 0 1 0 1 0 1 1
Golgi 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 1 2 1 1 0 0 0 2
Mitochondria 22 11 6 7 11 43 60 53 51 69 53 72 0 0 0 4 3 4 0 0 0 0 0 0
Nucleus 141 128 181 84 203 217 305 300 241 224 172 248 125 271 190 166 151 102 137 132 176 70 217 241
Nuclear Periphery 0 1 1 0 0 2 0 4 2 2 0 5 0 2 2 0 1 0 0 0 0 0 0 1
Nucleolus 33 13 26 22 21 42 83 90 76 68 68 93 4 8 4 13 7 5 8 1 10 0 10 8
Peroxisomes 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 1 0 0 0 0 0 0 0 0
SpindlePole 0 0 0 1 0 0 0 2 0 0 0 0 0 1 4 0 0 1 0 1 0 0 0 2
Vac/Vac Membrane 0 0 3 1 0 1 2 2 3 5 1 1 0 6 0 2 6 4 4 2 4 1 9 15
Unique Cell Count 153 138 191 91 226 251 358 362 282 281 208 308 138 313 230 196 181 121 155 141 196 75 246 281
Labelled Cell Count 199 157 218 116 249 312 462 471 385 378 298 426 142 325 236 208 191 131 155 141 196 75 246 281


Nucleus

(Huh et al. Nature 2003 425:686)
Screen WT1 WT2 WT3 RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3 AF100 AF140 AF180
Mean Cell GFP Intensity (1e-4) 5.9 7.2 7.0 6.1 6.2 5.6 5.4 5.4 5.2 5.4 5.0 5.0 8.6 8.5 8.2 10.3 9.9 9.8 6.6 8.2 8.7
Std Deviation (1e-4) 1.0 1.6 1.1 1.3 1.6 1.3 1.3 1.4 1.2 1.5 1.1 1.1 1.7 2.1 2.4 2.6 2.0 2.3 1.1 1.4 1.5
Intensity Change (Log2) – – – -0.19 -0.16 -0.3 -0.36 -0.36 -0.43 -0.37 -0.49 -0.47 0.31 0.28 0.23 0.56 0.51 0.49 -0.09 0.23 0.32


Localization RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3
Cortical Patches 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Bud 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Bud Neck 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Bud Site 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Cell Periphery 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Cytoplasm 0 2.6 0 0 0 0 0 0 0 3.8 4.5 5.4 3.9 4.1 0
Endoplasmic Reticulum 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Endosome 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Golgi 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Mitochondria 0 0.9 4.6 4.7 4.2 4.9 6.2 6.3 6.1 0 0 0 0 0 0
Nucleus -0.8 -1.9 -2.9 -3.4 -4.0 -3.2 -4.6 -3.8 -4.5 -1.5 -2.9 -3.8 -3.3 -3.5 -3.1
Nuclear Periphery 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Nucleolus 2.2 -1.4 0.9 2.7 3.1 3.5 2.8 4.5 4.2 -3.3 -4.8 -4.7 -2.3 -3.3 -2.7
Peroxisomes 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
SpindlePole 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Vacuole 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0


Endocytosis
Temp Actin Patch (Sac6-tdTomato) Cortical Patch (Sla1-GFP) Late Endosome (Snf7-GFP) Vacuole (Vph1-GFP)
37℃
RT
Cell Cycle Omics

CYCLoPs (Mpe1-GFP)
Gene / Allele Actin Patch (Sac6-tdTomato) Cortical Patch (Sla1-GFP) Late Endosome (Snf7-GFP) Vacuole (Sac6-tdTomato)
Gene Images
Gene Images
Images are not yet available
Images are not yet available