Standard name
Description Subunit of the ASTRA complex, involved in chromatin remodeling; telomere length regulator involved in the stability or biogenesis of PIKKs such as TORC1; similar to S. pombe Tti1p; detected in highly purified mitochondria in high-throughput studies

Micrographs

ensLOC DeepLoc
Localization WT1 WT2 WT3 RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3 WT1 WT2 WT3 AF100 AF140 AF180
Cortical Patches 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Bud 0 0 0 0 0 0 0 0 0 0 0.05 0 0 0 0 0 0 0 0 0 0 0 0 0
Bud Neck 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Bud Site 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Cell Periphery 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0.08 0 0.05 0 0 0 0 0 0
Cytoplasm 0.98 0.99 0.89 0.96 0.97 0.96 0.88 0.85 0.8 0.75 0.74 0.73 0.98 1.0 0.99 0.93 0.93 0.96 0.94 0.93 0.85 0.89 0.9 0.87
Endoplasmic Reticulum 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0.05 0.05 0 0 0 0 0 0 0
Endosome 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Golgi 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Mitochondria 0 0 0.19 0.17 0.11 0.18 0.3 0.35 0.47 0.49 0.44 0.56 0 0 0.05 0.06 0 0 0 0 0.09 0 0 0
Nucleus 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Nuclear Periphery 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Nucleolus 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Peroxisomes 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
SpindlePole 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Vac/Vac Membrane 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
ensLOC DeepLoc
Localization WT1 WT2 WT3 RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3 WT1 WT2 WT3 AF100 AF140 AF180
Cortical Patches 0 0 0 0 2 0 0 0 0 0 0 0 0 1 0 0 0 0 0 0 0 1 2 4
Bud 0 0 0 2 2 3 6 3 4 8 10 13 1 3 10 0 0 0 0 0 0 0 4 3
Bud Neck 0 0 0 0 1 1 1 4 2 0 2 1 0 0 0 0 0 0 0 0 0 0 1 1
Bud Site 0 0 0 0 0 0 1 1 0 1 2 0 0 0 0 0 0 0
Cell Periphery 2 0 1 4 2 0 3 2 0 5 1 5 1 1 3 28 8 17 0 0 0 0 0 0
Cytoplasm 237 90 101 233 390 277 314 451 231 281 160 291 128 358 370 327 179 350 234 106 101 120 292 338
Endoplasmic Reticulum 3 1 1 1 0 0 0 2 0 1 0 0 1 0 0 19 10 15 1 1 0 3 3 5
Endosome 0 0 0 0 0 0 1 0 0 0 0 0 0 0 0 8 4 3 4 0 1 0 1 2
Golgi 0 0 0 0 0 0 0 0 0 0 0 0 0 1 1 5 3 1 0 0 0 0 0 2
Mitochondria 5 3 21 42 45 52 106 187 136 183 95 225 0 0 20 20 3 12 6 4 10 3 4 14
Nucleus 0 0 0 0 1 1 2 2 0 1 2 2 0 0 1 2 1 1 0 0 0 0 1 1
Nuclear Periphery 0 0 0 0 0 0 1 0 1 0 0 1 0 0 1 1 0 0 0 0 0 0 0 1
Nucleolus 0 0 0 1 0 1 0 0 2 0 2 1 0 0 0 1 0 0 0 0 0 0 0 0
Peroxisomes 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 1
SpindlePole 0 0 0 1 0 0 0 2 0 1 2 5 0 0 0 1 0 1 0 0 0 0 1 1
Vac/Vac Membrane 1 0 1 0 0 1 0 1 0 2 0 4 0 0 0 7 6 11 1 0 1 1 3 3
Unique Cell Count 242 91 113 243 403 288 357 528 287 373 217 399 130 358 372 353 192 366 251 115 119 135 325 390
Labelled Cell Count 248 94 125 284 443 336 435 655 376 483 276 548 131 364 406 419 214 411 251 115 119 135 325 390


Cytoplasm

(Huh et al. Nature 2003 425:686)
Screen WT1 WT2 WT3 RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3 AF100 AF140 AF180
Mean Cell GFP Intensity (1e-4) 5.5 4.4 3.5 3.6 3.6 3.2 3.0 3.0 2.7 2.6 2.6 2.5 5.0 4.9 4.4 6.6 6.9 7.4 5.0 5.1 5.7
Std Deviation (1e-4) 0.6 0.7 0.4 1.1 0.8 0.9 0.7 0.6 1.0 0.6 0.7 0.9 0.9 0.8 1.0 2.0 1.8 1.8 1.0 1.3 1.3
Intensity Change (Log2) 0.05 0.06 -0.1 -0.23 -0.2 -0.35 -0.41 -0.39 -0.48 0.54 0.51 0.36 0.94 0.99 1.09 0.54 0.56 0.71


Localization RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3
Actin
Bud
Bud Neck
Bud Site
Cell Periphery
Cyto
Endoplasmic Reticulum
Endosome
Golgi
Mitochondria
Nuclear Periphery
Nuc
Nucleolus
Peroxisomes
SpindlePole
Vac
Cortical Patches
Cytoplasm
Nucleus
Vacuole


Endocytosis
Temp Actin Patch (Sac6-tdTomato) Cortical Patch (Sla1-GFP) Late Endosome (Snf7-GFP) Vacuole (Vph1-GFP)
37℃
RT
Cell Cycle Omics

CYCLoPs (Tti1-GFP)
Gene / Allele Actin Patch (Sac6-tdTomato) Cortical Patch (Sla1-GFP) Late Endosome (Snf7-GFP) Vacuole (Sac6-tdTomato)
Gene Images
Gene Images
Images are not yet available
Images are not yet available