Standard name
Human Ortholog
Description Essential subunit of the Dam1 complex (aka DASH complex); complex couples kinetochores to the force produced by MT depolymerization thereby aiding in chromosome segregation; also localized to nuclear side of spindle pole body
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Micrographs

ensLOC DeepLoc
Localization WT1 WT2 WT3 RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3 WT1 WT2 WT3 AF100 AF140 AF180
Cortical Patches 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 – – – 0 0 0 0 0 0
Bud 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 – – – 0.05 0 0 0 0 0
Bud Neck 0 0 0 0 0.05 0 0 0 0 0 0 0 0 0 0 – – – 0 0 0 0 0 0
Bud Site 0.05 0 0 0 0 0 0 0 0 0 0 0 0 0 0 – – – – – – – – –
Cell Periphery 0 0 0 0.06 0 0 0 0 0 0 0 0 0 0 0 – – – 0 0 0.06 0 0 0
Cytoplasm 0.08 0.39 0.98 0.95 0.96 0.95 0.93 0.92 0.79 0.84 0.72 0.76 0.99 0.97 1.0 – – – 0 0.28 0.63 0.77 0.77 0.71
Endoplasmic Reticulum 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 – – – 0 0 0 0 0 0
Endosome 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 – – – 0 0 0 0 0 0
Golgi 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 – – – 0 0 0 0 0 0
Mitochondria 0.06 0.06 0 0 0 0.05 0.07 0.09 0.34 0.21 0.39 0.28 0 0 0 – – – 0 0 0.14 0 0 0.11
Nucleus 0.05 0 0 0 0 0 0 0 0 0 0 0 0 0 0 – – – 0 0 0 0 0 0
Nuclear Periphery 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 – – – 0 0 0 0 0 0
Nucleolus 0.49 0.18 0 0 0 0 0 0 0 0 0 0 0 0 0 – – – 0 0 0 0 0 0
Peroxisomes 0.08 0.07 0 0 0 0 0 0 0 0 0 0 0 0 0 – – – 0 0 0 0 0 0
SpindlePole 0.73 0.46 0 0 0 0 0 0 0 0 0 0 0 0 0 – – – 0.79 0.53 0 0 0 0
Vac/Vac Membrane 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 – – – 0 0 0 0 0 0
ensLOC DeepLoc
Localization WT1 WT2 WT3 RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3 WT1 WT2 WT3 AF100 AF140 AF180
Cortical Patches 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 – – – 1 1 5 2 2 2
Bud 0 1 0 0 0 0 1 0 1 5 3 5 0 0 0 – – – 10 8 2 0 2 3
Bud Neck 3 6 5 3 10 5 4 4 1 5 4 3 0 0 1 – – – 5 7 0 0 0 1
Bud Site 10 5 0 0 0 0 0 0 0 0 0 0 0 0 0 – – – – – – – – –
Cell Periphery 0 8 3 6 2 2 0 3 4 3 0 1 4 1 2 – – – 0 2 8 0 3 1
Cytoplasm 15 99 179 103 178 210 212 185 154 155 126 161 91 147 171 – – – 2 73 94 55 113 71
Endoplasmic Reticulum 0 0 0 0 1 0 0 0 1 1 0 0 0 0 0 – – – 0 0 1 0 2 0
Endosome 4 2 0 0 0 1 2 0 1 0 0 0 0 0 0 – – – 5 4 1 1 1 0
Golgi 0 0 2 0 0 0 0 0 0 0 0 0 1 1 0 – – – 0 0 1 0 0 0
Mitochondria 11 15 0 3 1 11 15 19 65 38 67 59 1 1 0 – – – 1 4 20 1 4 11
Nucleus 9 5 1 0 0 1 2 3 4 1 2 3 0 1 0 – – – 1 1 0 0 0 0
Nuclear Periphery 0 0 0 0 0 0 0 0 1 0 0 0 0 0 0 – – – 0 0 0 0 1 0
Nucleolus 96 46 0 0 0 0 1 1 0 2 0 1 0 1 0 – – – 6 8 0 0 0 0
Peroxisomes 15 19 0 0 0 0 0 0 0 0 0 2 0 0 0 – – – 1 1 0 0 0 0
SpindlePole 143 117 0 1 5 2 8 5 3 6 4 7 0 0 0 – – – 169 138 1 0 2 1
Vac/Vac Membrane 3 3 2 1 0 1 3 2 1 3 2 4 0 2 0 – – – 2 2 1 2 1 0
Unique Cell Count 197 255 182 108 186 220 229 201 194 185 174 211 92 152 171 213 263 149 72 147 101
Labelled Cell Count 309 326 192 117 197 233 248 222 236 219 208 246 97 154 174 213 263 149 72 147 101


SpindlePole

(Huh et al. Nature 2003 425:686)
Screen WT1 WT2 WT3 RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3 AF100 AF140 AF180
Mean Cell GFP Intensity (1e-4) 5.9 4.6 4.6 4.3 5.0 3.9 4.3 4.0 3.3 4.1 3.4 3.8 4.7 5.1 4.6 3.2 3.8 4.0 4.5 5.5 5.8
Std Deviation (1e-4) 1.3 1.2 1.3 2.2 2.4 1.4 2.3 1.4 1.7 2.0 1.0 1.5 1.1 2.0 1.8 1.2 0.9 0.5 1.7 2.4 3.2
Intensity Change (Log2) – – – -0.11 0.11 -0.26 -0.12 -0.22 -0.47 -0.17 -0.46 -0.29 0.01 0.15 -0.02 -0.53 -0.28 -0.21 -0.04 0.24 0.32


Localization RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3
Actin – – – – – – – – – – – – – – –
Bud – – – – – – – – – – – – – – –
Bud Neck – – – – – – – – – – – – – – –
Bud Site – – – – – – – – – – – – – – –
Cell Periphery – – – – – – – – – – – – – – –
Cyto – – – – – – – – – – – – – – –
Endoplasmic Reticulum – – – – – – – – – – – – – – –
Endosome – – – – – – – – – – – – – – –
Golgi – – – – – – – – – – – – – – –
Mitochondria – – – – – – – – – – – – – – –
Nuclear Periphery – – – – – – – – – – – – – – –
Nuc – – – – – – – – – – – – – – –
Nucleolus – – – – – – – – – – – – – – –
Peroxisomes – – – – – – – – – – – – – – –
SpindlePole – – – – – – – – – – – – – – –
Vac – – – – – – – – – – – – – – –
Cortical Patches – – – – – – – – – – – – – – –
Cytoplasm – – – – – – – – – – – – – – –
Nucleus – – – – – – – – – – – – – – –
Vacuole – – – – – – – – – – – – – – –

Images

Circle plot not available

Protein Concentration and Protein Localization Data

R1 R2 R3
G1 Pre-START G1 Post-START S/G2 Metaphase Anaphase Telophase G1 Pre-START G1 Post-START S/G2 Metaphase Anaphase Telophase G1 Pre-START G1 Post-START S/G2 Metaphase Anaphase Telophase
Concentration – – – – – – – – – – – – – – – – – –
Actin 0.1096 0.0003 0.0101 0.0023 0.0269 0.0003 – – – – – – 0.1339 0.0001 0.0098 0.001 0.0172 0.0015
Bud 0.0005 0.0031 0.0006 0.0022 0.0006 0 – – – – – – 0.0083 0.001 0.0027 0.0001 0.0005 0.0001
Bud Neck 0.0009 0.0001 0.001 0.0003 0.0026 0.0004 – – – – – – 0.0306 0.0001 0.0002 0.0001 0.0005 0.0002
Bud Periphery 0.0005 0.0026 0.0014 0.0111 0.0008 0 – – – – – – 0.0105 0.0013 0.0047 0.0001 0.0005 0.0003
Bud Site 0.0047 0.0028 0.0178 0.002 0.0015 0 – – – – – – 0.1066 0.0051 0.0018 0.0002 0.0016 0.0001
Cell Periphery 0.0002 0.0001 0.0004 0.0001 0.0003 0 – – – – – – 0.0042 0.0001 0.0001 0 0.0001 0
Cytoplasm 0.0303 0.015 0.0308 0.0104 0.0356 0.0453 – – – – – – 0.1491 0.0389 0.0438 0.0279 0.0686 0.0694
Cytoplasmic Foci 0.2098 0.0048 0.0058 0.0054 0.0471 0.002 – – – – – – 0.0224 0.0042 0.0031 0.0025 0.0319 0.0045
Eisosomes 0.001 0.0003 0.0003 0.0003 0.0003 0.0002 – – – – – – 0.0027 0.0001 0.0004 0.0001 0.0001 0.0001
Endoplasmic Reticulum 0.0043 0.0005 0.0039 0.0002 0.0066 0.0001 – – – – – – 0.0058 0.0005 0.0006 0.0003 0.0002 0.0005
Endosome 0.0525 0.0055 0.0135 0.0005 0.0776 0.0001 – – – – – – 0.0246 0.0006 0.0005 0.0042 0.0116 0.0056
Golgi 0.1282 0.002 0.0061 0.0005 0.0597 0 – – – – – – 0.0262 0.0001 0.0009 0.0087 0.0207 0.0043
Lipid Particles 0.1008 0.0003 0.0034 0.0021 0.0406 0 – – – – – – 0.0626 0.0006 0.0004 0.0014 0.0075 0.0023
Mitochondria 0.0683 0.0006 0.0277 0.0079 0.0316 0.0001 – – – – – – 0.1579 0.0002 0.0006 0.0469 0.0441 0.0059
None 0.0748 0.9581 0.8563 0.9109 0.6021 0.9508 – – – – – – 0.1193 0.9432 0.923 0.8969 0.763 0.9015
Nuclear Periphery 0.0036 0.0004 0.0053 0.0003 0.001 0 – – – – – – 0.0159 0.0005 0.0015 0.0002 0.0001 0.0006
Nucleolus 0.0014 0.0003 0.0004 0.0005 0.0001 0 – – – – – – 0.0146 0.0003 0.0002 0.0001 0.0001 0.0001
Nucleus 0.0012 0.0008 0.0054 0.0003 0.0003 0.0002 – – – – – – 0.0219 0.0006 0.0017 0.0002 0.0002 0.0009
Peroxisomes 0.1812 0.001 0.0054 0.0407 0.0583 0.0001 – – – – – – 0.0533 0.0001 0.0024 0.008 0.0305 0.0003
Punctate Nuclear 0.0243 0.001 0.0009 0.0016 0.0006 0.0002 – – – – – – 0.0151 0.002 0.0012 0.0002 0.0005 0.0008
Vacuole 0.0008 0.0003 0.0023 0.0002 0.0032 0 – – – – – – 0.0111 0.0004 0.0003 0.0003 0.0004 0.0005
Vacuole Periphery 0.001 0.0001 0.001 0.0001 0.0027 0 – – – – – – 0.0034 0.0001 0.0001 0.0004 0.0002 0.0005

Sequencing Data

R1 R2
G1 Post-START S/G2 Metaphase Anaphase Telophase G1 Post-START S/G2 Metaphase Anaphase Telophase
Gene Expression 24.2302 12.2186 12.3637 13.2287 16.1105 12.4188 14.8812 18.1926 10.6834 18.1008
Translational Efficiency 1.0105 1.6844 0.7354 0.9697 1.3112 1.7566 1.3889 1.0729 1.4022 1.4669

Hit Data

Dataset Hit
Protein Abundance –
Protein Localization ✘
Gene Expression ✘
Translational Efficiency ✘

No result is found for the queried gene(s). Please try another query.


Endocytosis
Temp Actin Patch (Sac6-tdTomato) Cortical Patch (Sla1-GFP) Late Endosome (Snf7-GFP) Vacuole (Vph1-GFP)
37℃
RT
Cell Cycle Omics

CYCLoPs (Spc34-GFP)
Gene / Allele Actin Patch (Sac6-tdTomato) Cortical Patch (Sla1-GFP) Late Endosome (Snf7-GFP) Vacuole (Sac6-tdTomato)
Gene Images
Gene Images
Images are not yet available
Images are not yet available