Standard name
Description Endonuclease involved in pre-rRNA processing at both ends of ITS2; functions with Grc3p in a conserved mechanism to modulate rRNA processing and ribosome biogenesis; may coordinate the action of the Rat1p-Rai1p exoRNAse; required for the G1/S cell cycle transition; human ortholog is Las1L; mutants require the SSD1-v allele for viability

Micrographs

ensLOC DeepLoc
Localization WT1 WT2 WT3 RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3 WT1 WT2 WT3 AF100 AF140 AF180
Cortical Patches 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Bud 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Bud Neck 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Bud Site 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Cell Periphery 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Cytoplasm 0 0 0 0.06 0.05 0.07 0.11 0.08 0.09 0.08 0.07 0.08 0.1 0.13 0.17 0.07 0.07 0.05 0 0 0 0 0 0
Endoplasmic Reticulum 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Endosome 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Golgi 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Mitochondria 0.27 0.11 0.25 0.32 0.2 0.59 0.64 0.63 0.74 0.79 0.82 0.8 0 0 0.05 0.07 0 0.06 0 0 0 0 0 0
Nucleus 0.93 0.93 0.88 0.82 0.82 0.71 0.59 0.6 0.6 0.56 0.59 0.56 0.88 0.82 0.77 0.88 0.88 0.89 0.89 0.91 0.86 0.87 0.76 0.6
Nuclear Periphery 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Nucleolus 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Peroxisomes 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
SpindlePole 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Vac/Vac Membrane 0 0.05 0 0 0.05 0 0 0 0 0 0 0 0 0 0 0 0 0.05 0.05 0.05 0.08 0.05 0.14 0.21
ensLOC DeepLoc
Localization WT1 WT2 WT3 RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3 WT1 WT2 WT3 AF100 AF140 AF180
Cortical Patches 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Bud 1 0 0 0 1 1 2 6 5 10 2 3 0 0 0 0 1 0 3 2 1 0 3 3
Bud Neck 0 0 0 0 0 0 0 0 0 0 2 1 0 0 0 0 0 0 0 0 0 0 0 2
Bud Site 0 0 0 0 1 0 5 6 8 13 9 14 0 0 0 0 0 0
Cell Periphery 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 1 0 0 0 0 0 0 0
Cytoplasm 2 4 8 11 14 21 56 36 27 33 22 37 23 60 63 31 15 8 0 1 0 0 2 1
Endoplasmic Reticulum 0 0 0 1 1 0 0 1 0 0 0 0 1 0 1 4 2 0 0 0 1 0 1 2
Endosome 0 0 1 0 4 0 2 1 0 0 0 0 0 0 2 2 0 2 1 0 1 1 4 2
Golgi 0 0 0 0 1 0 0 0 0 0 0 0 0 1 0 1 1 0 0 0 2 0 1 0
Mitochondria 54 26 55 60 56 183 320 293 233 342 256 375 4 20 19 30 6 9 2 4 0 0 2 6
Nucleus 188 224 196 152 229 220 295 277 189 241 183 260 199 367 291 378 185 138 183 220 197 104 196 123
Nuclear Periphery 1 1 0 2 2 1 9 1 2 7 3 4 0 1 3 1 4 0 0 0 1 0 0 1
Nucleolus 0 1 0 0 1 2 4 1 2 4 2 0 0 4 4 4 0 1 1 0 1 1 5 7
Peroxisomes 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
SpindlePole 0 0 0 0 0 0 2 1 1 3 1 0 0 1 1 1 0 1 2 0 0 1 3 4
Vac/Vac Membrane 3 11 3 3 13 11 19 17 8 8 2 13 5 15 15 15 9 8 10 11 17 6 35 43
Unique Cell Count 202 241 224 185 278 310 500 463 315 431 311 466 225 448 376 432 210 155 205 243 229 121 258 207
Labelled Cell Count 249 267 263 229 323 439 714 640 475 661 482 707 232 469 399 467 224 167 205 243 229 121 258 207


Nucleus

(Huh et al. Nature 2003 425:686)
Screen WT1 WT2 WT3 RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3 AF100 AF140 AF180
Mean Cell GFP Intensity (1e-4) 4.4 4.9 4.5 3.9 4.4 3.6 3.1 3.3 3.2 2.9 3.0 2.9 5.6 5.2 5.1 6.0 7.3 7.9 5.1 5.0 6.0
Std Deviation (1e-4) 0.6 0.9 1.5 1.0 1.4 0.9 0.9 0.9 0.9 0.8 0.7 0.7 1.2 1.3 1.2 1.3 1.6 1.8 1.2 1.1 2.1
Intensity Change (Log2) -0.2 -0.03 -0.33 -0.52 -0.45 -0.48 -0.64 -0.57 -0.62 0.32 0.21 0.17 0.42 0.7 0.8 0.17 0.17 0.42


Localization RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3
Cortical Patches 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Bud 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Bud Neck 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Bud Site 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Cell Periphery 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Cytoplasm 1.1 0.8 1.6 3.3 2.1 2.3 2.0 1.7 2.2 2.8 4.0 4.8 1.9 1.7 0.8
Endoplasmic Reticulum 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Endosome 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Golgi 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Mitochondria 0 0 0 0 0 0 0 0 0 -7.1 -7.8 -7.0 -6.4 -6.5 -4.8
Nucleus -1.5 -1.6 -4.5 -7.6 -7.3 -7.0 -8.1 -7.2 -8.2 0.3 -1.8 -3.1 0 0.2 0.5
Nuclear Periphery 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Nucleolus 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Peroxisomes 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
SpindlePole 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Vacuole 0 2.1 1.6 1.8 1.7 0 0 0 1.2 0 1.5 1.8 1.6 1.9 0


Endocytosis
Temp Actin Patch (Sac6-tdTomato) Cortical Patch (Sla1-GFP) Late Endosome (Snf7-GFP) Vacuole (Vph1-GFP)
37℃
RT
Cell Cycle Omics

CYCLoPs (Las1-GFP)
Gene / Allele Actin Patch (Sac6-tdTomato) Cortical Patch (Sla1-GFP) Late Endosome (Snf7-GFP) Vacuole (Sac6-tdTomato)
Gene Images
Gene Images
Images are not yet available
Images are not yet available