Standard name
Human Ortholog
Description ATPase involved in protein folding and NLS-directed nuclear transport; member of HSP70 family; required for ubiquitin-dependent degradation of short-lived proteins; forms chaperone complex with Ydj1p; localized to nucleus, cytoplasm, cell wall; 98% identical to paralog Ssa2p with different functional specificity in propagation of yeast [URE3] prions, vacuolar-mediated degradations of gluconeogenesis enzymes; general targeting factor of Hsp104p to prion fibrils
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Micrographs

ensLOC DeepLoc
Localization WT1 WT2 WT3 RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3 WT1 WT2 WT3 AF100 AF140 AF180
Cortical Patches 0 0 0 0 0 0 0 – 0 0 – 0 0 0 0 – – 0 0 0 0 0 0 0
Bud 0 0 0 0 0 0 0 – 0 0 – 0 0 0 0 – – 0 0 0 0 0 0 0
Bud Neck 0 0 0 0 0 0 0 – 0 0 – 0 0 0 0 – – 0 0 0 0 0 0 0
Bud Site 0 0 0 0 0 0 0 – 0 0 – 0 0 0 0 – – 0 – – – – – –
Cell Periphery 0 0.22 0.32 0.21 0.27 0.41 0.6 – 0.37 0.63 – 0.52 0 0.07 0.22 – – 0.61 0 0.05 0.16 0 0 0
Cytoplasm 1.0 0.88 0.74 0.88 0.76 0.69 0.5 – 0.69 0.45 – 0.57 0.99 0.97 0.84 – – 0.44 0.97 0.92 0.78 0.96 0.91 0.9
Endoplasmic Reticulum 0 0 0 0 0 0 0 – 0 0 – 0 0 0 0 – – 0.22 0 0 0 0 0 0
Endosome 0 0 0 0 0 0 0 – 0 0 – 0 0 0 0 – – 0 0 0 0 0 0 0
Golgi 0 0 0 0 0 0 0 – 0 0 – 0 0 0 0 – – 0 0 0 0 0 0 0
Mitochondria 0 0 0 0 0 0 0 – 0 0 – 0 0 0 0 – – 0 0 0 0 0 0 0
Nucleus 0 0 0 0 0 0 0 – 0 0 – 0 0 0 0 – – 0 0 0 0 0 0 0
Nuclear Periphery 0 0 0 0 0 0 0 – 0 0 – 0 0 0 0 – – 0 0 0 0 0 0 0
Nucleolus 0 0 0 0 0 0 0 – 0 0 – 0 0 0 0 – – 0 0 0 0 0 0 0
Peroxisomes 0 0 0 0 0 0 0 – 0 0 – 0 0 0 0 – – 0 0 0 0 0 0 0
SpindlePole 0 0 0 0 0 0 0 – 0 0 – 0 0 0 0 – – 0 0 0 0 0 0 0
Vac/Vac Membrane 0 0 0 0 0 0 0 – 0 0 – 0 0 0 0 – – 0 0 0 0 0 0 0
ensLOC DeepLoc
Localization WT1 WT2 WT3 RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3 WT1 WT2 WT3 AF100 AF140 AF180
Cortical Patches 0 0 0 0 0 0 0 – 0 0 – 0 0 0 0 – – 0 0 0 0 0 0 0
Bud 0 2 0 0 0 0 0 – 0 0 – 0 0 1 1 – – 0 0 0 0 0 7 1
Bud Neck 0 0 0 0 0 0 0 – 0 0 – 0 0 0 0 – – 0 0 0 0 0 1 0
Bud Site 0 0 0 0 0 0 0 – 0 0 – 0 0 0 0 – – 0 – – – – – –
Cell Periphery 1 56 6 17 13 40 24 – 13 62 – 22 2 12 37 – – 11 0 17 14 3 2 6
Cytoplasm 201 222 14 72 37 67 20 – 24 44 – 24 122 173 139 – – 8 187 345 70 177 222 229
Endoplasmic Reticulum 0 0 0 0 0 0 0 – 0 0 – 0 0 1 0 – – 4 1 4 1 0 0 4
Endosome 0 0 0 0 0 0 0 – 0 0 – 0 0 0 0 – – 0 0 0 0 0 0 0
Golgi 0 0 0 0 0 0 0 – 0 0 – 0 0 0 0 – – 0 0 0 0 0 0 2
Mitochondria 0 0 0 0 0 0 0 – 0 0 – 0 0 0 0 – – 0 0 0 0 0 0 0
Nucleus 0 0 0 0 0 0 0 – 0 0 – 0 0 0 0 – – 0 0 1 0 0 1 0
Nuclear Periphery 0 0 0 0 0 0 0 – 0 0 – 0 0 0 0 – – 0 0 0 0 0 0 0
Nucleolus 0 0 0 0 0 0 0 – 0 0 – 0 0 0 0 – – 0 0 0 0 0 0 1
Peroxisomes 0 0 0 0 0 0 0 – 0 0 – 0 0 0 0 – – 0 0 0 0 0 0 0
SpindlePole 0 0 0 0 0 0 0 – 0 0 – 0 0 0 0 – – 0 0 0 0 0 0 0
Vac/Vac Membrane 0 0 0 1 0 0 0 – 1 1 – 0 0 0 0 – – 0 0 0 0 0 2 1
Unique Cell Count 201 252 19 82 49 97 40 35 98 42 123 178 165 18 194 375 90 184 245 256
Labelled Cell Count 202 280 20 90 50 107 44 38 107 46 124 187 177 23 194 375 90 184 245 256


Cytoplasm

(Huh et al. Nature 2003 425:686)
Screen WT1 WT2 WT3 RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3 AF100 AF140 AF180
Mean Cell GFP Intensity (1e-4) 348.6 510.5 543.4 453.9 505.1 589.7 579.2 – 595.4 609.4 – 586.0 342.3 431.4 468.3 – – 804.9 325.9 306.6 369.6
Std Deviation (1e-4) 100.9 117.2 168.4 160.7 184.4 134.4 144.1 – 122.6 123.2 – 159.1 113.2 171.8 156.7 – – 167.9 87.9 133.6 144.6
Intensity Change (Log2) – – – -0.26 -0.11 0.12 0.09 – 0.13 0.17 – 0.11 -0.67 -0.33 -0.21 – – 0.57 -0.74 -0.83 -0.56


Localization RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3
Cortical Patches 0 0 0 0 – 0 0 – 0 0 0 0 – – 0
Bud 0 0 0 0 – 0 0 – 0 0 0 0 – – 0
Bud Neck 0 0 0 0 – 0 0 – 0 0 0 0 – – 0
Bud Site 0 0 0 0 – 0 0 – 0 0 0 0 – – 0
Cell Periphery 0 -0.4 0.8 2.0 – 0.4 2.6 – 1.5 0 0 0 – – 1.8
Cytoplasm 1.6 0.2 -0.4 -1.7 – -0.4 -2.3 – -1.2 5.1 4.4 1.2 – – -1.8
Endoplasmic Reticulum 0 0 0 0 – 0 0 – 0 0 0 0 – – 0
Endosome 0 0 0 0 – 0 0 – 0 0 0 0 – – 0
Golgi 0 0 0 0 – 0 0 – 0 0 0 0 – – 0
Mitochondria 0 0 0 0 – 0 0 – 0 0 0 0 – – 0
Nucleus 0 0 0 0 – 0 0 – 0 0 0 0 – – 0
Nuclear Periphery 0 0 0 0 – 0 0 – 0 0 0 0 – – 0
Nucleolus 0 0 0 0 – 0 0 – 0 0 0 0 – – 0
Peroxisomes 0 0 0 0 – 0 0 – 0 0 0 0 – – 0
SpindlePole 0 0 0 0 – 0 0 – 0 0 0 0 – – 0
Vacuole 0 0 0 0 – 0 0 – 0 0 0 0 – – 0

Images

Circle plot not available

Protein Concentration and Protein Localization Data

R1 R2 R3
G1 Pre-START G1 Post-START S/G2 Metaphase Anaphase Telophase G1 Pre-START G1 Post-START S/G2 Metaphase Anaphase Telophase G1 Pre-START G1 Post-START S/G2 Metaphase Anaphase Telophase
Concentration 201.934 285.5071 233.9489 257.1014 103.5791 211.7491 222.1611 332.9959 276.4777 284.2103 250.9735 282.6179 207.7018 321.0981 248.4881 215.8753 261.5909 317.7993
Actin 0.0027 0.0002 0.001 0.001 0.0014 0.0051 0.0059 0.0002 0.0043 0.0048 0.0008 0.0003 0.0288 0.0001 0.0095 0.0171 0 0.0005
Bud 0.0014 0.0003 0.0008 0.0019 0.0026 0.0016 0.0015 0.0002 0.0004 0.0006 0.0007 0.0002 0.0005 0.0001 0.0073 0.0008 0 0.0001
Bud Neck 0.0003 0.0001 0.0003 0.0006 0.0008 0.001 0.0004 0.0001 0.0002 0.0003 0.0003 0.0003 0.0019 0.0001 0.0006 0.0027 0.0001 0.0011
Bud Periphery 0.0003 0.0001 0.0003 0.0003 0.0014 0.0004 0.0006 0 0.0002 0.0003 0.0005 0.0001 0.0004 0 0.0051 0.0007 0 0
Bud Site 0.0013 0.0001 0.0005 0.0004 0.0009 0.0003 0.0009 0.0005 0.0004 0.0003 0.0001 0 0.0029 0.0001 0.0066 0.0014 0 0
Cell Periphery 0.0008 0.0003 0.0008 0.0002 0.0022 0.0001 0.0008 0.0017 0.0004 0.0001 0.0007 0.0001 0.0006 0.0001 0.0009 0.0001 0 0.0001
Cytoplasm 0.8733 0.9924 0.9271 0.981 0.7365 0.7856 0.837 0.9848 0.9206 0.9502 0.8142 0.9443 0.8118 0.9956 0.852 0.7827 0.9946 0.9889
Cytoplasmic Foci 0.0161 0.0005 0.0141 0.0017 0.0362 0.0137 0.0174 0.0005 0.0114 0.0013 0.0042 0.0004 0.0128 0.0003 0.0286 0.0596 0.0001 0.0003
Eisosomes 0.0005 0 0.0002 0 0.0008 0 0.0005 0.0007 0.0005 0 0.0001 0 0.0004 0 0.0008 0.0003 0 0
Endoplasmic Reticulum 0.0007 0.0001 0.0005 0.0002 0.0048 0.002 0.0045 0.0001 0.0039 0.0061 0.008 0.0009 0.003 0 0.0007 0.0074 0.0001 0.0004
Endosome 0.0017 0 0.0006 0.0003 0.0061 0.0017 0.0116 0 0.0193 0.0095 0.0186 0.0002 0.0186 0 0.0087 0.0328 0 0
Golgi 0.0011 0 0.0002 0.0001 0.0009 0.0003 0.0036 0 0.0019 0.0009 0.0006 0 0.0215 0 0.0011 0.0137 0 0
Lipid Particles 0.0073 0.0001 0.0014 0.0001 0.0091 0.0009 0.0018 0.0003 0.004 0.0001 0.0018 0 0.01 0 0.004 0.0196 0 0
Mitochondria 0.0046 0.0002 0.0049 0.0024 0.0054 0.0006 0.0121 0.0005 0.0066 0.0053 0.0046 0.0006 0.0052 0.0001 0.0191 0.0044 0 0.0002
None 0.0092 0.0025 0.0038 0.001 0.0472 0.002 0.0166 0.0017 0.004 0.0007 0.0094 0.004 0.0108 0.0021 0.009 0.0161 0.0002 0.0003
Nuclear Periphery 0.0194 0.0004 0.0035 0.0013 0.0243 0.0182 0.0283 0.0013 0.0063 0.0086 0.078 0.0088 0.0168 0.0002 0.0048 0.007 0.0014 0.0025
Nucleolus 0.0156 0.0004 0.0152 0.0004 0.0173 0.0032 0.0118 0.0028 0.0029 0 0.0052 0.0004 0.0094 0 0.0125 0.0007 0 0
Nucleus 0.0312 0.0015 0.0172 0.0046 0.0339 0.0225 0.0344 0.0029 0.0053 0.0087 0.0128 0.0361 0.0209 0.001 0.0088 0.0043 0.0032 0.005
Peroxisomes 0.0027 0 0.0002 0.0001 0.0006 0.0003 0.0005 0 0.001 0.0002 0.0003 0 0.0013 0 0.0039 0.0132 0 0
Punctate Nuclear 0.0043 0.0001 0.0031 0.0001 0.0089 0.1386 0.0031 0.0001 0.0009 0.0004 0.0007 0.0006 0.0176 0 0.0018 0.0124 0.0001 0.0001
Vacuole 0.0034 0.0004 0.0024 0.0014 0.0409 0.0012 0.0042 0.0012 0.0033 0.0008 0.0175 0.0016 0.0024 0.0001 0.0089 0.0015 0.0001 0.0002
Vacuole Periphery 0.0021 0.0001 0.002 0.0009 0.0179 0.0007 0.0023 0.0002 0.002 0.0009 0.021 0.0011 0.0027 0 0.0054 0.0014 0 0.0001

Sequencing Data

R1 R2
G1 Post-START S/G2 Metaphase Anaphase Telophase G1 Post-START S/G2 Metaphase Anaphase Telophase
Gene Expression 2209.9549 3094.6338 4273.2178 3722.1203 3190.1389 2626.3524 3535.8727 4040.6779 5030.3336 3261.4395
Translational Efficiency 0.9116 0.9372 0.853 1.1208 0.9623 0.8353 0.9454 1.0021 0.9405 1.0524

Hit Data

Dataset Hit
Protein Concentration ✘
Protein Localization ✘
Gene Expression ✔
Translational Efficiency ✘
Standard name
Human Ortholog
Description ATPase involved in protein folding and NLS-directed nuclear transport; member of HSP70 family; required for ubiquitin-dependent degradation of short-lived proteins; forms chaperone complex with Ydj1p; localized to nucleus, cytoplasm, cell wall; 98% identical to paralog Ssa2p with different functional specificity in propagation of yeast [URE3] prions, vacuolar-mediated degradations of gluconeogenesis enzymes; general targeting factor of Hsp104p to prion fibrils
Localization
Cell Percentages cytoplasm (98%)
Cell Cycle Regulation No
Subcompartmental Group cytoplasm-1

Ssa1

Ssa1


Nearest neighbours ( genes)

correlation threshold

0.6

1.0

0.80


Biological Process (Gene Ontology)
Molecular Function (Gene Ontology)
Cellular Component (Gene Ontology)

Endocytosis
Temp Actin Patch (Sac6-tdTomato) Cortical Patch (Sla1-GFP) Late Endosome (Snf7-GFP) Vacuole (Vph1-GFP)
37℃
RT
Cell Cycle Omics

CYCLoPs (Ssa1-GFP)
Gene / Allele Actin Patch (Sac6-tdTomato) Cortical Patch (Sla1-GFP) Late Endosome (Snf7-GFP) Vacuole (Sac6-tdTomato)
Gene Images
Gene Images
Images are not yet available
Images are not yet available