Standard name | |
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Description | Dynamin-related GTPase involved in mitochondrial organization; required for mitochondrial fission and inheritance; self assembles on the cytoplasmic face of mitochondrial tubules at sites where division will occur; participates in endocytosis and regulates peroxisome fission along with Vps1p; mutants in the human ortholog DNM1L, which mediates mitochondrial fission, peroxisomal division, autophagy, and mitophagy, are associated with slowly progressive infantile encephalopathy |
ensLOC | DeepLoc | |||||||||||||||||||||||
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Localization | WT1 | WT2 | WT3 | RAP60 | RAP140 | RAP220 | RAP300 | RAP380 | RAP460 | RAP540 | RAP620 | RAP700 | HU80 | HU120 | HU160 | rpd3Δ_1 | rpd3Δ_2 | rpd3Δ_3 | WT1 | WT2 | WT3 | AF100 | AF140 | AF180 |
Cortical Patches | 4 | 3 | 6 | 3 | 4 | 2 | 1 | 7 | 0 | 3 | 3 | 7 | 11 | 9 | 5 | 11 | 12 | 10 | 7 | 18 | 16 | 2 | 5 | 8 |
Bud | 9 | 19 | 38 | 36 | 38 | 48 | 77 | 58 | 52 | 77 | 76 | 79 | 6 | 5 | 10 | 10 | 16 | 18 | 5 | 8 | 14 | 1 | 3 | 6 |
Bud Neck | 1 | 1 | 2 | 1 | 0 | 3 | 5 | 7 | 11 | 7 | 3 | 2 | 0 | 1 | 0 | 0 | 2 | 0 | 7 | 14 | 6 | 4 | 8 | 8 |
Bud Site | 11 | 13 | 34 | 26 | 22 | 27 | 42 | 30 | 46 | 44 | 45 | 42 | 3 | 4 | 1 | 8 | 12 | 14 | – | – | – | – | – | – |
Cell Periphery | 1 | 0 | 2 | 1 | 1 | 1 | 0 | 0 | 0 | 0 | 1 | 0 | 0 | 0 | 0 | 0 | 1 | 1 | 0 | 0 | 0 | 0 | 0 | 0 |
Cytoplasm | 7 | 35 | 25 | 16 | 36 | 13 | 16 | 26 | 5 | 14 | 10 | 8 | 0 | 8 | 7 | 13 | 21 | 8 | 0 | 14 | 2 | 2 | 3 | 2 |
Endoplasmic Reticulum | 0 | 0 | 2 | 0 | 1 | 1 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 1 | 0 | 2 | 5 | 1 | 0 | 0 | 0 | 0 | 1 | 2 |
Endosome | 18 | 6 | 106 | 100 | 143 | 68 | 23 | 31 | 34 | 17 | 18 | 56 | 22 | 56 | 62 | 40 | 42 | 21 | 57 | 80 | 146 | 38 | 93 | 86 |
Golgi | 0 | 0 | 2 | 1 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 2 | 0 | 1 | 2 | 1 | 0 | 2 | 1 | 9 | 0 | 1 | 1 |
Mitochondria | 97 | 162 | 194 | 219 | 237 | 341 | 338 | 174 | 194 | 274 | 302 | 261 | 26 | 15 | 5 | 32 | 26 | 16 | 12 | 39 | 29 | 6 | 18 | 23 |
Nucleus | 4 | 4 | 6 | 7 | 7 | 17 | 20 | 22 | 13 | 16 | 7 | 7 | 0 | 0 | 1 | 2 | 5 | 4 | 0 | 1 | 0 | 0 | 0 | 1 |
Nuclear Periphery | 3 | 4 | 0 | 4 | 3 | 13 | 7 | 4 | 5 | 5 | 6 | 18 | 0 | 1 | 2 | 1 | 1 | 3 | 0 | 1 | 2 | 1 | 2 | 2 |
Nucleolus | 13 | 6 | 23 | 32 | 66 | 145 | 232 | 177 | 152 | 201 | 152 | 164 | 2 | 10 | 7 | 12 | 16 | 9 | 2 | 3 | 2 | 1 | 3 | 2 |
Peroxisomes | 48 | 17 | 268 | 235 | 198 | 194 | 147 | 141 | 179 | 293 | 275 | 437 | 35 | 72 | 82 | 92 | 119 | 81 | 71 | 37 | 240 | 50 | 126 | 160 |
SpindlePole | 40 | 26 | 184 | 149 | 166 | 153 | 257 | 307 | 228 | 319 | 234 | 310 | 25 | 77 | 60 | 71 | 87 | 62 | 23 | 32 | 89 | 23 | 79 | 69 |
Vac/Vac Membrane | 2 | 11 | 8 | 4 | 16 | 8 | 0 | 4 | 0 | 3 | 1 | 1 | 1 | 2 | 2 | 2 | 10 | 8 | 3 | 9 | 3 | 1 | 2 | 7 |
Unique Cell Count | 168 | 236 | 551 | 511 | 603 | 677 | 807 | 675 | 593 | 853 | 759 | 940 | 87 | 163 | 162 | 207 | 247 | 168 | 199 | 274 | 575 | 139 | 356 | 390 |
Labelled Cell Count | 258 | 307 | 900 | 834 | 938 | 1034 | 1165 | 988 | 919 | 1273 | 1133 | 1392 | 133 | 261 | 245 | 298 | 376 | 256 | 199 | 274 | 575 | 139 | 356 | 390 |
Screen | WT1 | WT2 | WT3 | RAP60 | RAP140 | RAP220 | RAP300 | RAP380 | RAP460 | RAP540 | RAP620 | RAP700 | HU80 | HU120 | HU160 | rpd3Δ_1 | rpd3Δ_2 | rpd3Δ_3 | AF100 | AF140 | AF180 |
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Mean Cell GFP Intensity (1e-4) | 5.3 | 4.5 | 5.0 | 5.3 | 5.5 | 4.9 | 4.8 | 5.6 | 5.5 | 5.5 | 5.7 | 6.2 | 6.4 | 6.4 | 6.6 | 6.8 | 6.9 | 6.6 | 5.6 | 5.8 | 6.1 |
Std Deviation (1e-4) | 0.8 | 1.1 | 1.2 | 1.5 | 1.9 | 1.3 | 1.3 | 1.6 | 1.5 | 1.4 | 1.4 | 1.6 | 1.1 | 1.0 | 1.0 | 1.4 | 1.4 | 1.2 | 1.1 | 1.2 | 1.4 |
Intensity Change (Log2) | – | – | – | 0.07 | 0.14 | -0.04 | -0.07 | 0.17 | 0.13 | 0.12 | 0.19 | 0.31 | 0.36 | 0.34 | 0.4 | 0.44 | 0.47 | 0.39 | 0.17 | 0.21 | 0.28 |
Localization | RAP60 | RAP140 | RAP220 | RAP300 | RAP380 | RAP460 | RAP540 | RAP620 | RAP700 | HU80 | HU120 | HU160 | rpd3Δ_1 | rpd3Δ_2 | rpd3Δ_3 |
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Actin | – | – | – | – | – | – | – | – | – | – | – | – | – | – | – |
Bud | – | – | – | – | – | – | – | – | – | – | – | – | – | 0 | – |
Bud Neck | – | – | – | – | – | – | – | – | – | – | – | – | – | 0 | – |
Bud Site | – | – | – | – | – | – | – | – | – | – | – | – | – | 0 | – |
Cell Periphery | – | – | – | – | – | – | – | – | – | – | – | – | – | 0 | – |
Cyto | – | – | – | – | – | – | – | – | – | – | – | – | – | – | – |
Endoplasmic Reticulum | – | – | – | – | – | – | – | – | – | – | – | – | – | 0 | – |
Endosome | – | – | – | – | – | – | – | – | – | – | – | – | – | 0 | – |
Golgi | – | – | – | – | – | – | – | – | – | – | – | – | – | 0 | – |
Mitochondria | – | – | – | – | – | – | – | – | – | – | – | – | – | 0 | – |
Nuclear Periphery | – | – | – | – | – | – | – | – | – | – | – | – | – | 0 | – |
Nuc | – | – | – | – | – | – | – | – | – | – | – | – | – | – | – |
Nucleolus | – | – | – | – | – | – | – | – | – | – | – | – | – | 0 | – |
Peroxisomes | – | – | – | – | – | – | – | – | – | – | – | – | – | 0 | – |
SpindlePole | – | – | – | – | – | – | – | – | – | – | – | – | – | 0 | – |
Vac | – | – | – | – | – | – | – | – | – | – | – | – | – | – | – |
Cortical Patches | – | – | – | – | – | – | – | – | – | – | – | – | – | 0 | – |
Cytoplasm | – | – | – | – | – | – | – | – | – | – | – | – | – | 0 | – |
Nucleus | – | – | – | – | – | – | – | – | – | – | – | – | – | 0 | – |
Vacuole | – | – | – | – | – | – | – | – | – | – | – | – | – | 2.3 | – |
Temp | Actin Patch (Sac6-tdTomato) | Cortical Patch (Sla1-GFP) | Late Endosome (Snf7-GFP) | Vacuole (Vph1-GFP) |
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37℃ | ||||
RT |
Gene / Allele | Actin Patch (Sac6-tdTomato) | Cortical Patch (Sla1-GFP) | Late Endosome (Snf7-GFP) | Vacuole (Sac6-tdTomato) |
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Gene | Images |
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Gene | Images |
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