Standard name
Description Dynamin-related GTPase involved in mitochondrial organization; required for mitochondrial fission and inheritance; self assembles on the cytoplasmic face of mitochondrial tubules at sites where division will occur; participates in endocytosis and regulates peroxisome fission along with Vps1p; mutants in the human ortholog DNM1L, which mediates mitochondrial fission, peroxisomal division, autophagy, and mitophagy, are associated with slowly progressive infantile encephalopathy
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Micrographs

ensLOC DeepLoc
Localization WT1 WT2 WT3 RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3 WT1 WT2 WT3 AF100 AF140 AF180
Cortical Patches 0 0 0 0 0 0 0 0 0 0 0 0 0.13 0.06 0 0.05 0.05 0.06 0 0.07 0 0 0 0
Bud 0.05 0.08 0.07 0.07 0.06 0.07 0.1 0.09 0.09 0.09 0.1 0.08 0.07 0 0.06 0.05 0.06 0.11 0 0 0 0 0 0
Bud Neck 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0.05 0 0 0 0
Bud Site 0.07 0.06 0.06 0.05 0 0 0.05 0 0.08 0.05 0.06 0 0 0 0 0 0.05 0.08 – – – – – –
Cell Periphery 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Cytoplasm 0 0.15 0.05 0 0.06 0 0 0 0 0 0 0 0 0.05 0 0.06 0.09 0.05 0 0.05 0 0 0 0
Endoplasmic Reticulum 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Endosome 0.11 0 0.19 0.2 0.24 0.1 0 0.05 0.06 0 0 0.06 0.25 0.34 0.38 0.19 0.17 0.12 0.29 0.29 0.26 0.28 0.26 0.22
Golgi 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Mitochondria 0.58 0.69 0.35 0.43 0.39 0.5 0.42 0.26 0.33 0.32 0.4 0.28 0.3 0.09 0 0.15 0.11 0.1 0.06 0.15 0.05 0.05 0.05 0.06
Nucleus 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Nuclear Periphery 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Nucleolus 0.08 0 0 0.06 0.11 0.21 0.29 0.26 0.26 0.24 0.2 0.17 0 0.06 0 0.06 0.06 0.05 0 0 0 0 0 0
Peroxisomes 0.29 0.07 0.49 0.46 0.33 0.29 0.18 0.21 0.3 0.34 0.36 0.46 0.4 0.44 0.51 0.44 0.48 0.48 0.36 0.14 0.42 0.36 0.36 0.41
SpindlePole 0.24 0.11 0.33 0.29 0.28 0.23 0.32 0.45 0.38 0.37 0.31 0.33 0.29 0.47 0.37 0.34 0.35 0.37 0.12 0.12 0.15 0.17 0.22 0.18
Vac/Vac Membrane 0 0.05 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0.05 0 0 0 0 0 0
ensLOC DeepLoc
Localization WT1 WT2 WT3 RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3 WT1 WT2 WT3 AF100 AF140 AF180
Cortical Patches 4 3 6 3 4 2 1 7 0 3 3 7 11 9 5 11 12 10 7 18 16 2 5 8
Bud 9 19 38 36 38 48 77 58 52 77 76 79 6 5 10 10 16 18 5 8 14 1 3 6
Bud Neck 1 1 2 1 0 3 5 7 11 7 3 2 0 1 0 0 2 0 7 14 6 4 8 8
Bud Site 11 13 34 26 22 27 42 30 46 44 45 42 3 4 1 8 12 14 – – – – – –
Cell Periphery 1 0 2 1 1 1 0 0 0 0 1 0 0 0 0 0 1 1 0 0 0 0 0 0
Cytoplasm 7 35 25 16 36 13 16 26 5 14 10 8 0 8 7 13 21 8 0 14 2 2 3 2
Endoplasmic Reticulum 0 0 2 0 1 1 0 0 0 0 0 0 0 1 0 2 5 1 0 0 0 0 1 2
Endosome 18 6 106 100 143 68 23 31 34 17 18 56 22 56 62 40 42 21 57 80 146 38 93 86
Golgi 0 0 2 1 0 0 0 0 0 0 0 0 2 0 1 2 1 0 2 1 9 0 1 1
Mitochondria 97 162 194 219 237 341 338 174 194 274 302 261 26 15 5 32 26 16 12 39 29 6 18 23
Nucleus 4 4 6 7 7 17 20 22 13 16 7 7 0 0 1 2 5 4 0 1 0 0 0 1
Nuclear Periphery 3 4 0 4 3 13 7 4 5 5 6 18 0 1 2 1 1 3 0 1 2 1 2 2
Nucleolus 13 6 23 32 66 145 232 177 152 201 152 164 2 10 7 12 16 9 2 3 2 1 3 2
Peroxisomes 48 17 268 235 198 194 147 141 179 293 275 437 35 72 82 92 119 81 71 37 240 50 126 160
SpindlePole 40 26 184 149 166 153 257 307 228 319 234 310 25 77 60 71 87 62 23 32 89 23 79 69
Vac/Vac Membrane 2 11 8 4 16 8 0 4 0 3 1 1 1 2 2 2 10 8 3 9 3 1 2 7
Unique Cell Count 168 236 551 511 603 677 807 675 593 853 759 940 87 163 162 207 247 168 199 274 575 139 356 390
Labelled Cell Count 258 307 900 834 938 1034 1165 988 919 1273 1133 1392 133 261 245 298 376 256 199 274 575 139 356 390


Mitochondria, Punctate Nuclear

(Huh et al. Nature 2003 425:686)
Screen WT1 WT2 WT3 RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3 AF100 AF140 AF180
Mean Cell GFP Intensity (1e-4) 5.3 4.5 5.0 5.3 5.5 4.9 4.8 5.6 5.5 5.5 5.7 6.2 6.4 6.4 6.6 6.8 6.9 6.6 5.6 5.8 6.1
Std Deviation (1e-4) 0.8 1.1 1.2 1.5 1.9 1.3 1.3 1.6 1.5 1.4 1.4 1.6 1.1 1.0 1.0 1.4 1.4 1.2 1.1 1.2 1.4
Intensity Change (Log2) – – – 0.07 0.14 -0.04 -0.07 0.17 0.13 0.12 0.19 0.31 0.36 0.34 0.4 0.44 0.47 0.39 0.17 0.21 0.28


Localization RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3
Actin – – – – – – – – – – – – – – –
Bud – – – – – – – – – – – – – 0 –
Bud Neck – – – – – – – – – – – – – 0 –
Bud Site – – – – – – – – – – – – – 0 –
Cell Periphery – – – – – – – – – – – – – 0 –
Cyto – – – – – – – – – – – – – – –
Endoplasmic Reticulum – – – – – – – – – – – – – 0 –
Endosome – – – – – – – – – – – – – 0 –
Golgi – – – – – – – – – – – – – 0 –
Mitochondria – – – – – – – – – – – – – 0 –
Nuclear Periphery – – – – – – – – – – – – – 0 –
Nuc – – – – – – – – – – – – – – –
Nucleolus – – – – – – – – – – – – – 0 –
Peroxisomes – – – – – – – – – – – – – 0 –
SpindlePole – – – – – – – – – – – – – 0 –
Vac – – – – – – – – – – – – – – –
Cortical Patches – – – – – – – – – – – – – 0 –
Cytoplasm – – – – – – – – – – – – – 0 –
Nucleus – – – – – – – – – – – – – 0 –
Vacuole – – – – – – – – – – – – – 2.3 –

Images

Circle plot not available

Protein Concentration and Protein Localization Data

R1 R2 R3
G1 Pre-START G1 Post-START S/G2 Metaphase Anaphase Telophase G1 Pre-START G1 Post-START S/G2 Metaphase Anaphase Telophase G1 Pre-START G1 Post-START S/G2 Metaphase Anaphase Telophase
Concentration 4.8406 8.3737 6.3525 4.282 4.2787 4.8677 6.4864 7.4471 6.9453 7.0978 5.9784 7.2232 5.5656 7.0875 6.3945 5.7664 4.7523 6.5378
Actin 0.0089 0.0687 0.0311 0.0245 0.0887 0.0119 0.0268 0.0481 0.0347 0.0074 0.0312 0.0331 0.0366 0.1514 0.0844 0.0159 0.0411 0.0428
Bud 0.0002 0.0004 0.0001 0.0002 0.0002 0 0.0001 0.0001 0.0002 0.0003 0.0013 0 0.0003 0.0004 0.0002 0.0002 0.0013 0
Bud Neck 0.0004 0.0005 0.0002 0.0003 0.0003 0.0001 0.0004 0.0002 0.0004 0.0005 0.0014 0.0002 0.0009 0.0004 0.0004 0.0003 0.0045 0.0001
Bud Periphery 0.0002 0.0003 0.0002 0.0002 0.0002 0.0001 0.0002 0.0001 0.0002 0.0005 0.0028 0.0001 0.001 0.0005 0.0003 0.0003 0.0017 0.0001
Bud Site 0.0022 0.0024 0.001 0.0005 0.0004 0.0001 0.001 0.0007 0.0009 0.0011 0.0014 0.0002 0.0022 0.0018 0.0008 0.0005 0.0214 0.0001
Cell Periphery 0.0001 0.0001 0.0042 0.0001 0 0 0 0 0 0.0001 0.0002 0 0.0025 0.0001 0 0.0001 0.0005 0
Cytoplasm 0.0002 0.0114 0.0002 0.0001 0.0001 0 0.0015 0.0001 0.0003 0 0.0021 0.0002 0.0059 0.0001 0.0001 0.0001 0.0011 0
Cytoplasmic Foci 0.0638 0.0774 0.0487 0.0629 0.0345 0.0343 0.0561 0.0631 0.0536 0.0745 0.0858 0.025 0.0404 0.0378 0.0454 0.0337 0.0159 0.0229
Eisosomes 0.0001 0.0001 0.0002 0.0001 0.0001 0.0001 0.0001 0.0001 0.0001 0.0001 0.0003 0.0001 0.0009 0.0003 0.0002 0.0001 0.0006 0.0002
Endoplasmic Reticulum 0.0001 0.0001 0 0 0 0 0.0007 0 0.0011 0 0.0009 0.0001 0.0073 0 0 0 0.0022 0
Endosome 0.0083 0.0134 0.0053 0.0239 0.0115 0.0021 0.0152 0.0048 0.0089 0.0019 0.0193 0.0065 0.0116 0.0106 0.0063 0.0139 0.0339 0.0065
Golgi 0.0309 0.0567 0.0383 0.1187 0.0583 0.0158 0.0242 0.0235 0.0277 0.009 0.0179 0.0212 0.0573 0.0594 0.0435 0.0496 0.0258 0.0651
Lipid Particles 0.1931 0.0919 0.0924 0.0884 0.0569 0.1421 0.0925 0.0684 0.0805 0.084 0.079 0.0692 0.1225 0.0709 0.0975 0.1391 0.0591 0.0712
Mitochondria 0.0306 0.0361 0.0607 0.0723 0.0619 0.0617 0.0269 0.0277 0.0363 0.0681 0.0672 0.0251 0.0964 0.1034 0.055 0.0704 0.2519 0.104
None 0.0003 0.0001 0 0 0 0 0.001 0 0.0001 0 0.0053 0.0002 0.0137 0.0001 0.0001 0.0001 0.0005 0
Nuclear Periphery 0.0001 0.0001 0.0001 0 0 0 0.0019 0 0.0018 0 0.0019 0.0006 0.0011 0 0.0002 0 0.0018 0
Nucleolus 0.003 0.0001 0.0003 0.0004 0.0001 0.0001 0.0019 0.0002 0.0001 0.0002 0.0007 0.0001 0.0024 0.0004 0.0002 0.0006 0.0007 0
Nucleus 0.0009 0.0001 0.0001 0.0001 0 0 0.0036 0 0.0003 0 0.0008 0.0001 0.0008 0.0001 0 0.0001 0.0012 0
Peroxisomes 0.6494 0.6378 0.714 0.6042 0.6861 0.7309 0.743 0.762 0.7518 0.7517 0.6681 0.8157 0.59 0.5611 0.6617 0.672 0.5282 0.6865
Punctate Nuclear 0.0043 0.0003 0.0009 0.0003 0.0001 0.0003 0.0018 0.0007 0.0004 0.0002 0.0106 0.0022 0.0042 0.0007 0.0031 0.0003 0.0014 0.0002
Vacuole 0.0021 0.001 0.0016 0.0014 0.0002 0.0001 0.0004 0.0001 0.0004 0.0002 0.0012 0.0001 0.0009 0.0003 0.0001 0.0018 0.0033 0.0001
Vacuole Periphery 0.001 0.0009 0.0004 0.0012 0.0002 0.0002 0.0006 0.0001 0.0003 0.0001 0.0006 0.0001 0.001 0.0004 0.0003 0.0011 0.0018 0.0002

Sequencing Data

R1 R2
G1 Post-START S/G2 Metaphase Anaphase Telophase G1 Post-START S/G2 Metaphase Anaphase Telophase
Gene Expression 25.2318 26.8933 18.6685 29.0985 28.3103 27.6426 35.3054 31.8211 35.3839 31.653
Translational Efficiency 1.6647 1.9397 1.7346 1.4012 1.2125 1.7907 1.7053 1.5692 1.5077 1.4287

Hit Data

Dataset Hit
Protein Abundance ✘
Protein Localization ✘
Gene Expression ✘
Translational Efficiency ✔