Standard name
Description Membrane protein involved in zinc ion homeostasis; member of the four-protein IZH family, expression induced by zinc deficiency; deletion reduces sensitivity to elevated zinc and shortens lag phase, overexpression reduces Zap1p activity

Micrographs

ensLOC DeepLoc
Localization WT1 WT2 WT3 RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3 WT1 WT2 WT3 AF100 AF140 AF180
Cortical Patches 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0.05 0.06 0 0 0
Bud 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Bud Neck 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Bud Site 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Cell Periphery 0 0 0 0.06 0 0 0 0 0 0 0 0 0 0 0 0 0.05 0 0 0 0 0 0 0.06
Cytoplasm 0.7 0.87 0.92 0.85 0.91 0.81 0.77 0.75 0.67 0.7 0.6 0.63 0.99 0.99 0.99 0.91 0.86 0.84 0.19 0.45 0.39 0.68 0.73 0.56
Endoplasmic Reticulum 0.12 0.1 0 0 0 0 0 0 0 0 0 0 0 0 0 0.08 0.13 0.1 0.52 0.21 0.09 0.06 0 0.07
Endosome 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Golgi 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Mitochondria 0.25 0 0.07 0.23 0.12 0.39 0.41 0.34 0.52 0.49 0.5 0.63 0 0 0 0.05 0 0 0.2 0.09 0.26 0.08 0.05 0.09
Nucleus 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Nuclear Periphery 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Nucleolus 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Peroxisomes 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
SpindlePole 0 0 0 0 0 0 0 0 0 0 0.05 0 0 0 0 0 0 0 0 0 0 0 0 0
Vac/Vac Membrane 0.08 0 0 0 0 0 0 0 0 0 0 0.05 0 0 0 0 0 0 0 0.06 0 0 0 0.05
ensLOC DeepLoc
Localization WT1 WT2 WT3 RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3 WT1 WT2 WT3 AF100 AF140 AF180
Cortical Patches 1 4 1 0 0 0 0 0 0 0 0 0 0 0 0 0 0 1 1 14 10 3 4 6
Bud 0 2 6 2 1 1 5 4 10 4 0 1 0 0 0 0 0 1 1 4 1 1 5 4
Bud Neck 0 1 1 0 0 0 1 1 2 0 0 0 0 0 0 1 2 2 0 12 5 1 2 4
Bud Site 0 0 0 0 0 0 0 0 0 1 0 1 0 0 0 0 0 0
Cell Periphery 0 8 2 9 5 3 0 5 2 2 0 0 0 1 0 2 7 3 0 10 6 1 5 8
Cytoplasm 28 206 132 131 179 163 167 215 161 174 12 27 146 151 125 212 133 84 12 127 66 70 109 85
Endoplasmic Reticulum 5 23 1 1 1 1 0 0 2 2 0 0 1 0 0 19 20 10 33 59 15 6 5 11
Endosome 1 0 0 0 3 0 0 1 0 0 0 0 0 0 0 5 3 3 0 2 5 3 1 1
Golgi 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 2 1 0 0 0 0 0 0 1
Mitochondria 10 5 10 35 24 78 88 99 124 123 10 27 0 3 0 12 5 4 13 25 44 7 7 13
Nucleus 0 1 1 1 0 0 0 1 0 2 0 0 1 0 1 5 4 1 0 0 1 0 1 0
Nuclear Periphery 1 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 1 0 0 0 0
Nucleolus 0 0 0 1 0 0 0 0 0 1 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Peroxisomes 0 0 0 0 1 0 0 2 0 0 0 0 0 0 0 0 0 2 0 0 0 0 0 0
SpindlePole 0 0 0 0 2 1 6 6 6 3 1 1 0 1 0 4 2 4 0 0 0 1 0 0
Vac/Vac Membrane 3 0 2 4 1 4 2 3 1 2 0 2 0 1 0 3 6 2 2 16 3 3 3 8
Unique Cell Count 40 237 143 154 197 200 216 287 240 249 20 43 147 153 126 232 155 100 65 282 170 104 150 152
Labelled Cell Count 49 250 156 184 217 251 269 337 308 314 23 59 148 157 126 265 183 117 65 282 170 104 150 152


Endoplasmic Reticulum

(Huh et al. Nature 2003 425:686)
Screen WT1 WT2 WT3 RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3 AF100 AF140 AF180
Mean Cell GFP Intensity (1e-4) 4.8 5.1 4.3 4.4 4.4 3.5 2.9 3.4 3.2 3.0 3.2 3.2 4.6 4.1 4.3 5.5 6.5 6.3 4.5 4.6 5.7
Std Deviation (1e-4) 0.7 0.8 1.3 1.1 2.6 1.0 0.9 1.4 1.7 1.7 1.5 2.1 1.6 1.6 2.0 2.3 2.4 2.6 1.3 1.6 1.8
Intensity Change (Log2) 0.06 0.05 -0.3 -0.57 -0.34 -0.4 -0.51 -0.41 -0.43 0.1 -0.06 0.0 0.37 0.62 0.57 0.09 0.1 0.41


Localization RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3
Cortical Patches 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Bud 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Bud Neck 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Bud Site 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Cell Periphery 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Cytoplasm -2.0 -0.5 -2.8 -3.7 -4.3 -5.6 -5.2 -4.2 -4.8 3.0 2.7 2.7 -0.3 -1.8 -2.0
Endoplasmic Reticulum 0 0 0 0 0 0 0 0 0 0 0 0 3.1 4.1 0
Endosome 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Golgi 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Mitochondria 0 0 0 0 0 0 0 0 0 0 0 0 -0.7 -1.5 -1.0
Nucleus 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Nuclear Periphery 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Nucleolus 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Peroxisomes 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
SpindlePole 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Vacuole 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0


Endocytosis
Temp Actin Patch (Sac6-tdTomato) Cortical Patch (Sla1-GFP) Late Endosome (Snf7-GFP) Vacuole (Vph1-GFP)
37℃
RT
Cell Cycle Omics

CYCLoPs (Izh3-GFP)
Gene / Allele Actin Patch (Sac6-tdTomato) Cortical Patch (Sla1-GFP) Late Endosome (Snf7-GFP) Vacuole (Sac6-tdTomato)
Gene Images
Gene Images
Images are not yet available
Images are not yet available