Standard name
Human Ortholog
Description Membrane protein involved in zinc ion homeostasis; member of the four-protein IZH family, expression induced by zinc deficiency; deletion reduces sensitivity to elevated zinc and shortens lag phase, overexpression reduces Zap1p activity

Micrographs

ensLOC DeepLoc
Localization WT1 WT2 WT3 RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3 WT1 WT2 WT3 AF100 AF140 AF180
Cortical Patches 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0.05 0.06 0 0 0
Bud 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Bud Neck 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Bud Site 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Cell Periphery 0 0 0 0.06 0 0 0 0 0 0 0 0 0 0 0 0 0.05 0 0 0 0 0 0 0.06
Cytoplasm 0.7 0.87 0.92 0.85 0.91 0.81 0.77 0.75 0.67 0.7 0.6 0.63 0.99 0.99 0.99 0.91 0.86 0.84 0.19 0.45 0.39 0.68 0.73 0.56
Endoplasmic Reticulum 0.12 0.1 0 0 0 0 0 0 0 0 0 0 0 0 0 0.08 0.13 0.1 0.52 0.21 0.09 0.06 0 0.07
Endosome 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Golgi 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Mitochondria 0.25 0 0.07 0.23 0.12 0.39 0.41 0.34 0.52 0.49 0.5 0.63 0 0 0 0.05 0 0 0.2 0.09 0.26 0.08 0.05 0.09
Nucleus 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Nuclear Periphery 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Nucleolus 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Peroxisomes 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
SpindlePole 0 0 0 0 0 0 0 0 0 0 0.05 0 0 0 0 0 0 0 0 0 0 0 0 0
Vac/Vac Membrane 0.08 0 0 0 0 0 0 0 0 0 0 0.05 0 0 0 0 0 0 0 0.06 0 0 0 0.05
ensLOC DeepLoc
Localization WT1 WT2 WT3 RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3 WT1 WT2 WT3 AF100 AF140 AF180
Cortical Patches 1 4 1 0 0 0 0 0 0 0 0 0 0 0 0 0 0 1 1 14 10 3 4 6
Bud 0 2 6 2 1 1 5 4 10 4 0 1 0 0 0 0 0 1 1 4 1 1 5 4
Bud Neck 0 1 1 0 0 0 1 1 2 0 0 0 0 0 0 1 2 2 0 12 5 1 2 4
Bud Site 0 0 0 0 0 0 0 0 0 1 0 1 0 0 0 0 0 0
Cell Periphery 0 8 2 9 5 3 0 5 2 2 0 0 0 1 0 2 7 3 0 10 6 1 5 8
Cytoplasm 28 206 132 131 179 163 167 215 161 174 12 27 146 151 125 212 133 84 12 127 66 70 109 85
Endoplasmic Reticulum 5 23 1 1 1 1 0 0 2 2 0 0 1 0 0 19 20 10 33 59 15 6 5 11
Endosome 1 0 0 0 3 0 0 1 0 0 0 0 0 0 0 5 3 3 0 2 5 3 1 1
Golgi 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 2 1 0 0 0 0 0 0 1
Mitochondria 10 5 10 35 24 78 88 99 124 123 10 27 0 3 0 12 5 4 13 25 44 7 7 13
Nucleus 0 1 1 1 0 0 0 1 0 2 0 0 1 0 1 5 4 1 0 0 1 0 1 0
Nuclear Periphery 1 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 1 0 0 0 0
Nucleolus 0 0 0 1 0 0 0 0 0 1 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Peroxisomes 0 0 0 0 1 0 0 2 0 0 0 0 0 0 0 0 0 2 0 0 0 0 0 0
SpindlePole 0 0 0 0 2 1 6 6 6 3 1 1 0 1 0 4 2 4 0 0 0 1 0 0
Vac/Vac Membrane 3 0 2 4 1 4 2 3 1 2 0 2 0 1 0 3 6 2 2 16 3 3 3 8
Unique Cell Count 40 237 143 154 197 200 216 287 240 249 20 43 147 153 126 232 155 100 65 282 170 104 150 152
Labelled Cell Count 49 250 156 184 217 251 269 337 308 314 23 59 148 157 126 265 183 117 65 282 170 104 150 152


Endoplasmic Reticulum

(Huh et al. Nature 2003 425:686)
Screen WT1 WT2 WT3 RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3 AF100 AF140 AF180
Mean Cell GFP Intensity (1e-4) 4.8 5.1 4.3 4.4 4.4 3.5 2.9 3.4 3.2 3.0 3.2 3.2 4.6 4.1 4.3 5.5 6.5 6.3 4.5 4.6 5.7
Std Deviation (1e-4) 0.7 0.8 1.3 1.1 2.6 1.0 0.9 1.4 1.7 1.7 1.5 2.1 1.6 1.6 2.0 2.3 2.4 2.6 1.3 1.6 1.8
Intensity Change (Log2) 0.06 0.05 -0.3 -0.57 -0.34 -0.4 -0.51 -0.41 -0.43 0.1 -0.06 0.0 0.37 0.62 0.57 0.09 0.1 0.41


Localization RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3
Cortical Patches 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Bud 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Bud Neck 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Bud Site 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Cell Periphery 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Cytoplasm -2.0 -0.5 -2.8 -3.7 -4.3 -5.6 -5.2 -4.2 -4.8 3.0 2.7 2.7 -0.3 -1.8 -2.0
Endoplasmic Reticulum 0 0 0 0 0 0 0 0 0 0 0 0 3.1 4.1 0
Endosome 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Golgi 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Mitochondria 0 0 0 0 0 0 0 0 0 0 0 0 -0.7 -1.5 -1.0
Nucleus 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Nuclear Periphery 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Nucleolus 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Peroxisomes 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
SpindlePole 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Vacuole 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0

Images

Circle plot not available

Protein Concentration and Protein Localization Data

R1 R2 R3
G1 Pre-START G1 Post-START S/G2 Metaphase Anaphase Telophase G1 Pre-START G1 Post-START S/G2 Metaphase Anaphase Telophase G1 Pre-START G1 Post-START S/G2 Metaphase Anaphase Telophase
Concentration -0.241 0.9149 0.4575 0.5355 -0.0176 0.4918 3.009 1.8323 1.5704 1.4394 0.9547 1.2845 2.3293 2.5443 2.7732 3.0915 2.6605 2.5591
Actin 0.0013 0.0265 0.0066 0.0051 0.0007 0.0021 0.0229 0.0015 0.0347 0.0067 0.0007 0.0046 0.0232 0.0138 0.0292 0.0069 0.0026 0.0082
Bud 0.0002 0.0008 0.0036 0.0002 0 0.0004 0.0007 0.0152 0.0003 0.0016 0.0011 0 0.0011 0.0055 0.0025 0.0002 0.0003 0.0022
Bud Neck 0.0002 0.0015 0.0041 0.0004 0.0004 0.0015 0.0002 0.0004 0.0014 0.0009 0.0004 0.0061 0.0049 0.0013 0.0049 0.003 0.0005 0.0039
Bud Periphery 0.0004 0.002 0.0017 0.0003 0.0001 0.0014 0.0011 0.0037 0.0006 0.01 0.0009 0.0001 0.004 0.0118 0.0051 0.0004 0.0005 0.0082
Bud Site 0.0014 0.0461 0.0211 0.0007 0.0001 0.0001 0.0009 0.0127 0.009 0.0024 0.0003 0.0001 0.0121 0.0427 0.0228 0.0039 0.0009 0.0009
Cell Periphery 0.0029 0.0117 0.0022 0.0008 0.0004 0.0006 0.0008 0.007 0.0027 0.0072 0.0009 0.0009 0.003 0.0025 0.0033 0.0012 0.001 0.0006
Cytoplasm 0.1577 0.2681 0.2686 0.2107 0.2743 0.3709 0.0737 0.4102 0.1737 0.2 0.358 0.3705 0.1576 0.2006 0.1725 0.2144 0.2052 0.2251
Cytoplasmic Foci 0.0626 0.0113 0.0146 0.0084 0.0047 0.0029 0.0097 0.0113 0.0173 0.0019 0.0952 0.0122 0.0364 0.0076 0.0138 0.0189 0.0141 0.0237
Eisosomes 0.0007 0.004 0.0006 0.0003 0.0009 0.0004 0.0009 0.0008 0.0009 0.0006 0.0004 0.0004 0.0009 0.0007 0.0042 0.0008 0.0002 0.0004
Endoplasmic Reticulum 0.0361 0.0377 0.0372 0.0124 0.0139 0.0024 0.0138 0.0297 0.014 0.031 0.0034 0.007 0.0092 0.0059 0.0149 0.0122 0.0023 0.0106
Endosome 0.0159 0.0028 0.0247 0.0065 0.0023 0.0006 0.0613 0.0023 0.0385 0.0018 0.0006 0.0012 0.0124 0.0022 0.0129 0.0041 0.0193 0.0047
Golgi 0.0012 0.0012 0.0246 0.0037 0.0003 0.0002 0.0021 0.0013 0.0079 0.0004 0.0003 0.0005 0.0036 0.0006 0.0103 0.0021 0.0257 0.0016
Lipid Particles 0.0114 0.0081 0.0031 0.0123 0.0006 0.0002 0.0094 0.0028 0.0051 0.0019 0.0044 0.0006 0.0086 0.0012 0.0059 0.0014 0.0015 0.0033
Mitochondria 0.0024 0.0007 0.0048 0.0682 0.0002 0.0003 0.0076 0.0022 0.001 0.0003 0.0189 0.0002 0.0064 0.0038 0.0039 0.0006 0.0199 0.0005
None 0.6654 0.5702 0.5708 0.6552 0.6992 0.6141 0.5811 0.4692 0.6769 0.7149 0.4231 0.5904 0.6945 0.6942 0.6781 0.7161 0.6995 0.6979
Nuclear Periphery 0.0207 0.0009 0.0027 0.0005 0.0005 0.0002 0.1655 0.0019 0.0025 0.0047 0.0047 0.0006 0.0065 0.0006 0.0031 0.0035 0.0002 0.0003
Nucleolus 0.0011 0.0007 0.0004 0.0001 0.0001 0.0001 0.0014 0.0031 0.0005 0.0004 0.0185 0.0006 0.0012 0.0005 0.0015 0.0003 0.0001 0.0001
Nucleus 0.0031 0.0011 0.0014 0.0005 0.0005 0.0003 0.0224 0.0103 0.0011 0.0031 0.01 0.0012 0.0017 0.0013 0.0019 0.003 0.0003 0.0005
Peroxisomes 0.002 0.0007 0.0011 0.011 0.0001 0.0001 0.001 0.0004 0.002 0.0001 0.0027 0.0002 0.0058 0.0004 0.002 0.0038 0.0048 0.0045
Punctate Nuclear 0.0038 0.0007 0.0009 0.0003 0.0003 0.0001 0.0082 0.0114 0.0057 0.0007 0.0522 0.0018 0.0043 0.0008 0.004 0.0008 0.0002 0.0019
Vacuole 0.0059 0.003 0.004 0.0023 0.0005 0.0007 0.0066 0.0025 0.0035 0.009 0.0013 0.0005 0.0019 0.0022 0.0023 0.0021 0.0008 0.0009
Vacuole Periphery 0.0035 0.0001 0.0013 0.0002 0.0001 0.0002 0.0087 0.0003 0.0008 0.0004 0.0017 0.0001 0.0007 0.0001 0.0009 0.0002 0.0002 0.0001

Sequencing Data

R1 R2
G1 Post-START S/G2 Metaphase Anaphase Telophase G1 Post-START S/G2 Metaphase Anaphase Telophase
Gene Expression 18.2549 19.3407 17.9396 24.1146 16.6554 18.9205 28.722 29.2843 25.8358 27.2464
Translational Efficiency 0.4571 0.6505 0.6837 0.4158 0.5623 0.664 0.5487 0.4363 0.5323 0.4297

Hit Data

Dataset Hit
Protein Concentration
Protein Localization
Gene Expression
Translational Efficiency
Standard name
Human Ortholog
Description Membrane protein involved in zinc ion homeostasis; member of the four-protein IZH family, expression induced by zinc deficiency; deletion reduces sensitivity to elevated zinc and shortens lag phase, overexpression reduces Zap1p activity
Localization
Cell Percentages ER (42%)
Cell Cycle Regulation No
Subcompartmental Group ER-3

Izh3

Izh3


Nearest neighbours ( genes)

correlation threshold

0.6

1.0

0.80


Biological Process (Gene Ontology)

Molecular Function (Gene Ontology)

Cellular Component (Gene Ontology)


Endocytosis
Temp Actin Patch (Sac6-tdTomato) Cortical Patch (Sla1-GFP) Late Endosome (Snf7-GFP) Vacuole (Vph1-GFP)
37℃
RT
Cell Cycle Omics

CYCLoPs (Izh3-GFP)
Gene / Allele Actin Patch (Sac6-tdTomato) Cortical Patch (Sla1-GFP) Late Endosome (Snf7-GFP) Vacuole (Sac6-tdTomato)
Gene Images
Gene Images
Images are not yet available
Images are not yet available