Standard name
Description One of four subunits of the ESCRT-III complex; involved in the sorting of transmembrane proteins into the multivesicular body (MVB) pathway; recruited from the cytoplasm to endosomal membranes; ESCRT-III stands for endosomal sorting complex required for transport III

Micrographs

Sub-cellular Localization

ensLOC DeepLoc
Localization WT1 WT2 WT3 RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3 WT1 WT2 WT3 AF100 AF140 AF180
Cortical Patches 0.10 0 0 0 0.09 0.05 0 0 0.10 0.06 0 0
Bud 0.14 0 0 0 0 0 0 0 0 0 0 0
Bud Neck 0 0 0 0 0 0 0 0 0.06 0 0 0
Bud Site 0 0 0 0 0 0 0 0
Cell Periphery 0 0 0 0 0 0 0 0 0 0 0 0
Cytoplasm 0 0.16 0.06 0.12 0 0 0.12 0.12 0 0 0 0
Endoplasmic Reticulum 0 0 0 0 0 0 0 0 0 0 0 0
Endosome 0.10 0.05 0.06 0.16 0.30 0.08 0 0.12 0.45 0.47 0.50 0.98
Golgi 0.05 0 0 0 0.09 0 0 0 0.05 0.12 0 0
Mitochondria 0.33 0.68 0.72 0.68 0.57 0.87 0.76 0.06 0.06 0 0 0
Nucleus 0 0 0 0 0 0 0 0.06 0 0 0 0
Nuclear Periphery 0 0 0 0 0 0 0 0 0 0 0 0
Nucleolus 0.10 0.32 0.28 0.24 0.17 0.16 0.06 0.69 0 0 0 0
Peroxisomes 0.38 0 0.06 0 0.13 0.08 0.06 0 0.11 0.24 0 0
SpindlePole 0.33 0.05 0.06 0 0.09 0 0.12 0.25 0.07 0 0 0
Vac/Vac Membrane 0 0.05 0 0 0 0.08 0 0 0 0 0 0
ensLOC DeepLoc
Localization WT1 WT2 WT3 RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3 WT1 WT2 WT3 AF100 AF140 AF180
Cortical Patches 2 0 1 0 2 2 0 0 1 2 0 0
Bud 3 0 0 0 1 0 0 0 0 1 0 0
Bud Neck 0 0 0 0 0 0 0 0 1 0 0 0
Bud Site 0 0 0 0 0 0 0 0
Cell Periphery 0 0 0 0 0 0 0 0 0 0 0 0
Cytoplasm 0 3 2 3 0 1 2 2 0 0 0 0
Endoplasmic Reticulum 0 0 0 0 0 0 0 0 0 0 0 0
Endosome 2 1 2 4 7 3 0 2 8 23 1 2
Golgi 1 0 0 1 2 1 0 0 0 5 0 0
Mitochondria 7 13 26 17 13 33 13 1 1 1 0 0
Nucleus 0 0 1 0 0 0 0 1 0 0 0 0
Nuclear Periphery 0 0 1 0 0 1 0 0 0 0 0 0
Nucleolus 2 6 10 6 4 6 1 11 0 0 0 0
Peroxisomes 8 0 2 1 3 3 1 0 2 12 0 0
SpindlePole 7 1 2 1 2 1 2 4 1 1 0 0
Vac/Vac Membrane 0 1 1 0 0 3 0 0 0 0 0 0
Unique Cell Count 21 19 36 25 23 38 17 16 19 51 2 3
Labelled Cell Count 32 25 48 33 34 54 19 21 19 51 2 3

Yeast GFP Assignment

(Huh et al. Nature 2003 425:686 )

Endosome

Protein Abundance

Screen WT1 WT2 WT3 RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3 AF100 AF140 AF180
Mean Cell GFP Intensity (1e-4) 9.8 10.7 10.6 11.1 12.8 12.4 11.6 15.9
Std Deviation (1e-4) 5.0 2.7 2.8 3.2 3.6 3.5 3.5 5.5
Intensity Change (Log2) 0.13 0.12 0.19 0.39 0.35 0.25 0.71

Localization Change

Localization RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3
Cortical Patches 0 0 0 0 0 0 0
Bud 0 0 0 0 0 0 0
Bud Neck 0 0 0 0 0 0 0
Bud Site 0 0 0 0 0 0 0
Cell Periphery 0 0 0 0 0 0 0
Cytoplasm 0 0 0 0 0 0 0
Endoplasmic Reticulum 0 0 0 0 0 0 0
Endosome 0 0 0 0 0 0 0
Golgi 0 0 0 0 0 0 0
Mitochondria 2.2 2.9 2.3 1.5 4.2 2.6 0
Nucleus 0 0 0 0 0 0 0
Nuclear Periphery 0 0 0 0 0 0 0
Nucleolus 0 0 0 0 0 0 3.7
Peroxisomes 0 0 0 -1.9 0 0 0
SpindlePole 0 0 0 0 0 0 0
Vacuole 0 0 0 0 0 0 0