Standard name
Description Nucleolar protein involved in the early steps of 35S rRNA processing; interacts with Faf1p; member of a transcriptionally co-regulated set of genes called the RRB regulon; essential gene
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Micrographs

ensLOC DeepLoc
Localization WT1 WT2 WT3 RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3 WT1 WT2 WT3 AF100 AF140 AF180
Cortical Patches – 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Bud – 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0.05
Bud Neck – 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Bud Site – 0 0 0 0 0 0 0 0 0 0 0.05 0 0 0 0 0 0 – – – – – –
Cell Periphery – 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Cytoplasm – 0 0.16 0.18 0.22 0.27 0.18 0.18 0.18 0.14 0.14 0.16 0.37 0.33 0.41 0.5 0.39 0.55 0 0 0.1 0.32 0.22 0.2
Endoplasmic Reticulum – 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0.05 0 0 0 0 0 0
Endosome – 0 0 0 0 0 0.07 0.06 0 0 0.05 0.06 0 0 0 0 0 0 0 0 0 0 0 0
Golgi – 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Mitochondria – 0 0 0 0 0.22 0 0.07 0.14 0.15 0.16 0.17 0 0 0 0 0 0.05 0 0 0 0 0 0
Nucleus – 0.56 0.43 0.07 0.12 0.11 0.11 0.12 0.13 0.12 0.14 0.1 0.14 0.13 0.1 0.19 0.2 0.16 0.24 0.38 0.24 0.07 0.1 0.1
Nuclear Periphery – 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Nucleolus – 0.53 0.41 0.78 0.65 0.51 0.62 0.58 0.58 0.58 0.53 0.55 0.54 0.6 0.51 0.41 0.44 0.31 0.64 0.49 0.51 0.44 0.47 0.47
Peroxisomes – 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
SpindlePole – 0 0.06 0.42 0.26 0.14 0.37 0.34 0.27 0.41 0.31 0.38 0.07 0.18 0.14 0.05 0.1 0 0 0 0 0 0.05 0
Vac/Vac Membrane – 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0.05 0 0.05 0 0 0.06 0 0.06
ensLOC DeepLoc
Localization WT1 WT2 WT3 RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3 WT1 WT2 WT3 AF100 AF140 AF180
Cortical Patches – 0 0 0 0 0 0 1 0 0 0 0 0 0 0 0 0 0 0 1 0 0 0 0
Bud – 0 0 0 0 1 1 2 3 3 5 1 0 0 1 1 0 0 0 2 2 1 5 12
Bud Neck – 0 1 6 1 0 5 8 6 4 1 5 1 3 4 0 0 0 0 0 0 0 3 3
Bud Site – 0 0 0 0 5 3 7 1 4 6 12 0 0 0 0 0 1 – – – – – –
Cell Periphery – 0 0 0 0 0 0 0 0 0 0 0 0 0 0 1 1 0 0 0 0 0 1 1
Cytoplasm – 3 26 24 41 40 47 66 33 27 28 42 63 72 91 73 52 72 0 0 19 52 51 52
Endoplasmic Reticulum – 0 0 0 0 1 0 0 0 0 0 0 0 1 0 3 5 7 0 0 0 0 0 0
Endosome – 1 5 0 8 2 18 22 4 4 10 16 1 2 3 1 4 0 1 3 3 1 4 4
Golgi – 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 2 0 1 0 0
Mitochondria – 5 0 0 5 32 7 25 25 28 32 43 0 2 1 6 5 7 0 1 1 5 5 6
Nucleus – 104 70 9 23 16 30 43 23 22 28 25 24 28 23 27 27 21 10 79 47 12 23 26
Nuclear Periphery – 7 3 0 1 1 2 1 3 0 3 1 0 0 0 0 1 0 0 1 2 0 0 0
Nucleolus – 98 67 107 120 75 165 212 105 111 103 142 91 131 112 59 59 40 27 102 102 72 107 124
Peroxisomes – 3 0 0 0 0 1 4 0 0 4 3 2 1 1 1 1 0 0 0 0 0 0 0
SpindlePole – 1 9 58 49 21 98 124 48 78 60 97 12 39 32 7 13 3 0 2 7 5 12 10
Vac/Vac Membrane – 5 0 0 4 1 2 3 2 2 1 6 2 0 1 1 6 2 2 6 8 9 9 15
Unique Cell Count 186 162 137 186 146 267 366 180 190 195 256 169 218 221 145 133 131 44 209 199 165 229 263
Labelled Cell Count 227 181 204 252 195 379 518 253 283 281 393 196 279 269 180 174 153 44 209 199 165 229 263


Nucleolus

(Huh et al. Nature 2003 425:686)
Screen WT1 WT2 WT3 RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3 AF100 AF140 AF180
Mean Cell GFP Intensity (1e-4) – 10.0 9.4 9.2 8.4 5.4 6.5 6.4 5.8 5.8 5.6 5.6 8.4 7.6 7.4 8.3 9.4 7.3 7.4 8.5 8.3
Std Deviation (1e-4) – 2.6 2.8 3.1 2.9 2.2 2.0 2.2 2.1 1.8 2.0 1.8 3.5 3.3 3.2 4.7 5.3 4.3 3.5 4.0 3.4
Intensity Change (Log2) – – – -0.02 -0.16 -0.8 -0.53 -0.55 -0.68 -0.68 -0.73 -0.73 -0.16 -0.3 -0.34 -0.17 0.01 -0.37 -0.33 -0.13 -0.17


Localization RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3
Cortical Patches 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Bud 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Bud Neck 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Bud Site 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Cell Periphery 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Cytoplasm 0.3 1.4 2.4 0.4 0.6 0.6 -0.5 -0.4 0.1 4.4 3.7 5.3 6.4 4.5 7.0
Endoplasmic Reticulum 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Endosome 0 0.6 0 1.6 1.4 0 0 1.0 1.4 0 0 0 0 0 0
Golgi 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Mitochondria 0 0 6.3 0 3.4 4.9 5.1 5.4 5.5 0 0 0 0 0 0
Nucleus -7.2 -6.5 -6.3 -7.6 -8.1 -6.3 -6.7 -6.1 -7.9 -5.9 -6.7 -7.4 -4.6 -4.2 -5.0
Nuclear Periphery 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Nucleolus 6.4 4.3 1.8 4.1 3.5 3.1 3.2 2.2 2.8 2.3 3.6 1.8 -0.1 0.5 -1.9
Peroxisomes 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
SpindlePole 7.6 5.2 2.6 7.2 6.9 5.2 7.7 6.0 7.4 0.6 3.6 2.8 -0.3 1.4 -1.4
Vacuole 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0

Images

Circle plot not available

Protein Concentration and Protein Localization Data

R1 R2 R3
G1 Pre-START G1 Post-START S/G2 Metaphase Anaphase Telophase G1 Pre-START G1 Post-START S/G2 Metaphase Anaphase Telophase G1 Pre-START G1 Post-START S/G2 Metaphase Anaphase Telophase
Concentration 3.7087 4.3536 3.921 3.7237 3.7816 3.9697 5.4321 7.261 5.7724 5.1141 5.2198 6.1047 8.8414 9.613 8.5515 8.8219 8.4521 8.4125
Actin 0.0142 0.0003 0.0023 0.064 0 0.0023 0.0352 0 0.0171 0.0461 0.071 0.0127 0.003 0 0 0 0 0
Bud 0.0003 0.0001 0.0005 0.0001 0.0001 0.0001 0.0004 0.0001 0.0003 0.0028 0.0011 0.0001 0 0 0 0 0 0
Bud Neck 0.0006 0.0003 0.001 0.0022 0.0012 0.0005 0.0012 0.0005 0.0008 0.0072 0.0014 0.0004 0.0006 0.0003 0.0002 0.0005 0.0005 0.0003
Bud Periphery 0.0004 0.0002 0.001 0.0001 0.0002 0.0002 0.001 0.0001 0.0006 0.0068 0.0022 0.0001 0.0001 0 0 0 0.0001 0
Bud Site 0.0018 0.0005 0.0015 0.0006 0.0003 0.0003 0.0019 0.0006 0.0017 0.0133 0.0033 0.0002 0.0002 0.0002 0.0001 0.0002 0.0003 0.0001
Cell Periphery 0.0005 0.0002 0.0006 0.0001 0.0004 0.0001 0.0009 0.0005 0.0003 0.0005 0.0009 0.0001 0.0002 0.0001 0.0001 0.0002 0.0002 0.0001
Cytoplasm 0.0133 0 0.0061 0 0 0.0001 0.0031 0 0.0013 0.0013 0.0006 0.0003 0.0009 0 0 0 0.0001 0
Cytoplasmic Foci 0.0159 0.0003 0.0163 0.0037 0.0017 0.0024 0.022 0.0001 0.0124 0.0123 0.0085 0.0007 0.0031 0.0001 0.0002 0.0003 0.0028 0.0002
Eisosomes 0.0002 0 0.0001 0.0002 0 0 0.0004 0 0.0002 0.0001 0.0006 0.0001 0.0001 0 0 0 0 0
Endoplasmic Reticulum 0.0035 0 0.0014 0 0 0.0001 0.0076 0 0.0009 0.001 0.0012 0.0004 0.0021 0 0 0 0 0
Endosome 0.029 0.0001 0.0218 0.0002 0.0003 0.0075 0.0143 0 0.0056 0.0133 0.0341 0.0013 0.0029 0 0 0 0.0006 0
Golgi 0.0109 0.0001 0.0103 0.0014 0.0004 0.0082 0.005 0 0.0021 0.0113 0.0169 0.0009 0.0005 0 0 0 0.0002 0
Lipid Particles 0.0158 0.0017 0.0139 0.0015 0.0181 0.0047 0.0164 0.0002 0.0049 0.004 0.0445 0.0012 0.0022 0.0005 0.0003 0.0011 0.0112 0.0006
Mitochondria 0.0068 0.0114 0.0124 0.0005 0.0066 0.0172 0.0105 0.0001 0.0033 0.0111 0.018 0.0005 0.0003 0 0 0 0.0016 0
None 0.0236 0 0.0141 0.0001 0.0001 0 0.0024 0 0.0059 0.0006 0.0011 0.0002 0.0022 0 0 0 0.0002 0
Nuclear Periphery 0.0117 0 0.0043 0 0.0056 0.0002 0.025 0 0.0007 0.008 0.0027 0.0126 0.0014 0 0 0 0.0018 0
Nucleolus 0.7047 0.8307 0.776 0.7879 0.8231 0.8218 0.7209 0.8544 0.7887 0.7391 0.6889 0.817 0.8702 0.8838 0.8876 0.8911 0.7932 0.8951
Nucleus 0.1012 0.1476 0.1063 0.1192 0.1264 0.1277 0.1029 0.1403 0.145 0.0985 0.0793 0.1443 0.1047 0.1126 0.1014 0.1038 0.1324 0.089
Peroxisomes 0.0025 0.0007 0.0017 0.017 0.0034 0.0009 0.0112 0 0.0027 0.0051 0.0062 0.0003 0.0006 0 0 0.0001 0.0005 0
Punctate Nuclear 0.0348 0.0043 0.0025 0.0008 0.01 0.0044 0.011 0.0015 0.0042 0.0028 0.0087 0.0055 0.0041 0.0021 0.0096 0.0021 0.0526 0.0144
Vacuole 0.005 0.0011 0.0039 0.0002 0.0012 0.0007 0.003 0.0014 0.0011 0.0088 0.0049 0.0006 0.0004 0.0002 0.0003 0.0004 0.0008 0.0001
Vacuole Periphery 0.0034 0.0002 0.0021 0 0.0007 0.0004 0.0034 0.0001 0.0003 0.0059 0.0039 0.0006 0.0002 0 0 0 0.0007 0

Sequencing Data

R1 R2
G1 Post-START S/G2 Metaphase Anaphase Telophase G1 Post-START S/G2 Metaphase Anaphase Telophase
Gene Expression 42.1791 30.1644 34.6941 39.2584 39.3746 40.4695 33.1679 26.7604 33.0413 37.135
Translational Efficiency 2.5738 3.2492 2.5858 2.3749 2.5769 1.9889 1.6846 1.775 1.505 1.8223

Hit Data

Dataset Hit
Protein Abundance ✘
Protein Localization ✘
Gene Expression ✘
Translational Efficiency ✘