Standard name | |
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Human Ortholog | |
Description | Ribosomal 60S subunit protein L4A; N-terminally acetylated; homologous to mammalian ribosomal protein L4 and bacterial L4; RPL4A has a paralog, RPL4B, that arose from the whole genome duplication |
ensLOC | DeepLoc | |||||||||||||||||||||||
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Localization | WT1 | WT2 | WT3 | RAP60 | RAP140 | RAP220 | RAP300 | RAP380 | RAP460 | RAP540 | RAP620 | RAP700 | HU80 | HU120 | HU160 | rpd3Δ_1 | rpd3Δ_2 | rpd3Δ_3 | WT1 | WT2 | WT3 | AF100 | AF140 | AF180 |
Cortical Patches | – | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 2 | 0 | 1 | 1 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
Bud | – | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 1 | 0 | 0 | 0 | 0 | 1 | 1 | 0 | 0 | 0 | 0 | 0 | 0 |
Bud Neck | – | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
Bud Site | – | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | – | – | – | – | – | – |
Cell Periphery | – | 0 | 0 | 0 | 5 | 1 | 4 | 3 | 5 | 9 | 2 | 5 | 1 | 1 | 3 | 6 | 5 | 7 | 0 | 0 | 0 | 1 | 0 | 0 |
Cytoplasm | – | 16 | 33 | 91 | 166 | 80 | 182 | 170 | 101 | 124 | 34 | 107 | 184 | 209 | 271 | 437 | 373 | 376 | 36 | 60 | 36 | 77 | 162 | 217 |
Endoplasmic Reticulum | – | 2 | 1 | 2 | 5 | 4 | 15 | 19 | 14 | 17 | 3 | 18 | 8 | 6 | 3 | 23 | 24 | 22 | 5 | 1 | 3 | 8 | 22 | 31 |
Endosome | – | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
Golgi | – | 0 | 0 | 0 | 0 | 1 | 1 | 1 | 0 | 1 | 0 | 0 | 0 | 0 | 1 | 2 | 0 | 2 | 0 | 0 | 0 | 0 | 1 | 1 |
Mitochondria | – | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
Nucleus | – | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 1 | 0 | 0 | 0 | 1 | 0 | 0 |
Nuclear Periphery | – | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
Nucleolus | – | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
Peroxisomes | – | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
SpindlePole | – | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
Vac/Vac Membrane | – | 0 | 0 | 0 | 0 | 1 | 1 | 6 | 1 | 0 | 0 | 0 | 1 | 0 | 0 | 1 | 0 | 3 | 0 | 0 | 0 | 3 | 5 | 5 |
Unique Cell Count | 18 | 33 | 92 | 171 | 84 | 197 | 195 | 120 | 146 | 39 | 127 | 191 | 213 | 274 | 445 | 380 | 387 | 47 | 65 | 43 | 98 | 199 | 267 | |
Labelled Cell Count | 18 | 34 | 93 | 176 | 87 | 203 | 199 | 123 | 151 | 40 | 132 | 194 | 216 | 278 | 469 | 403 | 412 | 47 | 65 | 43 | 98 | 199 | 267 |
Screen | WT1 | WT2 | WT3 | RAP60 | RAP140 | RAP220 | RAP300 | RAP380 | RAP460 | RAP540 | RAP620 | RAP700 | HU80 | HU120 | HU160 | rpd3Δ_1 | rpd3Δ_2 | rpd3Δ_3 | AF100 | AF140 | AF180 |
---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|
Mean Cell GFP Intensity (1e-4) | – | 183.6 | 134.1 | 123.7 | 119.6 | 85.5 | 81.0 | 72.2 | 67.1 | 65.9 | 62.2 | 64.4 | 127.7 | 117.9 | 118.2 | 221.2 | 239.3 | 230.0 | 120.1 | 123.0 | 123.9 |
Std Deviation (1e-4) | – | 56.4 | 47.6 | 44.8 | 35.9 | 28.7 | 27.4 | 25.5 | 23.8 | 21.4 | 17.3 | 22.4 | 43.3 | 45.9 | 42.5 | 58.5 | 54.7 | 63.3 | 41.3 | 41.5 | 47.6 |
Intensity Change (Log2) | – | – | – | -0.12 | -0.16 | -0.65 | -0.73 | -0.89 | -1.0 | -1.02 | -1.11 | -1.06 | -0.07 | -0.19 | -0.18 | 0.72 | 0.84 | 0.78 | -0.16 | -0.12 | -0.11 |
Localization | RAP60 | RAP140 | RAP220 | RAP300 | RAP380 | RAP460 | RAP540 | RAP620 | RAP700 | HU80 | HU120 | HU160 | rpd3Δ_1 | rpd3Δ_2 | rpd3Δ_3 |
---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|
Cortical Patches | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
Bud | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
Bud Neck | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
Bud Site | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
Cell Periphery | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
Cytoplasm | -0.6 | -1.0 | -1.3 | -1.6 | -2.2 | -2.4 | -2.4 | -2.1 | -2.4 | -1.1 | -0.8 | -0.6 | -0.8 | -0.8 | -1.0 |
Endoplasmic Reticulum | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
Endosome | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
Golgi | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
Mitochondria | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
Nucleus | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
Nuclear Periphery | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
Nucleolus | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
Peroxisomes | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
SpindlePole | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
Vacuole | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
R1 | R2 | R3 | ||||||||||||||||
---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|
G1 Pre-START | G1 Post-START | S/G2 | Metaphase | Anaphase | Telophase | G1 Pre-START | G1 Post-START | S/G2 | Metaphase | Anaphase | Telophase | G1 Pre-START | G1 Post-START | S/G2 | Metaphase | Anaphase | Telophase | |
Concentration | 184.9623 | 262.4875 | 245.6387 | 198.321 | 170.7442 | 181.8554 | 183.2651 | 266.8849 | 247.8661 | 246.0294 | 233.3243 | 264.9506 | 206.0133 | 283.1063 | 246.3841 | 220.5094 | 261.9458 | 241.6994 |
Actin | 0.0052 | 0.0004 | 0.0026 | 0.0022 | 0.0024 | 0.0077 | 0.0074 | 0.0022 | 0.0022 | 0.0003 | 0.0029 | 0.0051 | 0.0551 | 0.0009 | 0.0172 | 0.0004 | 0.0001 | 0.0075 |
Bud | 0.0025 | 0.0011 | 0.0027 | 0.0024 | 0.0067 | 0.0033 | 0.0028 | 0.0013 | 0.0029 | 0.0023 | 0.0099 | 0.0047 | 0.0061 | 0.0051 | 0.007 | 0.0036 | 0.0017 | 0.0091 |
Bud Neck | 0.0009 | 0.0007 | 0.0011 | 0.001 | 0.0042 | 0.0035 | 0.0012 | 0.0008 | 0.0014 | 0.0007 | 0.0025 | 0.0021 | 0.0016 | 0.0006 | 0.0008 | 0.0014 | 0.0002 | 0.0042 |
Bud Periphery | 0.004 | 0.0008 | 0.0022 | 0.0026 | 0.0052 | 0.0032 | 0.0033 | 0.0008 | 0.0023 | 0.0008 | 0.0109 | 0.0028 | 0.011 | 0.0028 | 0.0066 | 0.0012 | 0.0005 | 0.006 |
Bud Site | 0.0014 | 0.0012 | 0.0019 | 0.0014 | 0.0019 | 0.0028 | 0.0019 | 0.0012 | 0.0018 | 0.0003 | 0.0019 | 0.0008 | 0.0064 | 0.0018 | 0.0034 | 0.0007 | 0.0001 | 0.0018 |
Cell Periphery | 0.0695 | 0.0403 | 0.074 | 0.0477 | 0.0186 | 0.0271 | 0.0546 | 0.0362 | 0.0398 | 0.0235 | 0.0394 | 0.0066 | 0.0679 | 0.0181 | 0.0244 | 0.0204 | 0.0148 | 0.0168 |
Cytoplasm | 0.4738 | 0.8192 | 0.6324 | 0.3252 | 0.5173 | 0.6206 | 0.4866 | 0.8556 | 0.7077 | 0.6989 | 0.3652 | 0.6593 | 0.4643 | 0.8762 | 0.7159 | 0.4081 | 0.4562 | 0.6848 |
Cytoplasmic Foci | 0.0106 | 0.001 | 0.0018 | 0.0184 | 0.0085 | 0.0085 | 0.0033 | 0.0007 | 0.0035 | 0.0008 | 0.0017 | 0.0028 | 0.0008 | 0.001 | 0.002 | 0.0011 | 0.0025 | 0.006 |
Eisosomes | 0.0015 | 0.0002 | 0.0002 | 0.0036 | 0.0014 | 0.0001 | 0.0038 | 0.0002 | 0 | 0 | 0.0001 | 0.0015 | 0.0062 | 0 | 0.0005 | 0 | 0 | 0.0002 |
Endoplasmic Reticulum | 0.0314 | 0.0092 | 0.0074 | 0.007 | 0.0114 | 0.0211 | 0.0343 | 0.0043 | 0.0074 | 0.0011 | 0.0034 | 0.0095 | 0.0071 | 0.0017 | 0.0035 | 0.0013 | 0.0002 | 0.004 |
Endosome | 0.0098 | 0.0022 | 0.0058 | 0.0074 | 0.0056 | 0.0353 | 0.0075 | 0.0013 | 0.0048 | 0.0016 | 0.0103 | 0.007 | 0.0035 | 0.0013 | 0.004 | 0.0036 | 0.0035 | 0.0129 |
Golgi | 0.0023 | 0.0006 | 0.0017 | 0.001 | 0.0008 | 0.0038 | 0.0018 | 0.0003 | 0.0017 | 0.0001 | 0.0029 | 0.0016 | 0.0015 | 0.0003 | 0.0016 | 0.0002 | 0.0001 | 0.0019 |
Lipid Particles | 0.0073 | 0.0015 | 0.004 | 0.012 | 0.0074 | 0.0049 | 0.0048 | 0.0007 | 0.0027 | 0.0004 | 0.0021 | 0.0009 | 0.0024 | 0.0006 | 0.0014 | 0.0006 | 0.0004 | 0.0038 |
Mitochondria | 0.0103 | 0.0011 | 0.0039 | 0.0195 | 0.0497 | 0.008 | 0.0207 | 0.0014 | 0.0066 | 0.0006 | 0.0166 | 0.0781 | 0.0431 | 0.0039 | 0.0473 | 0.0016 | 0.0013 | 0.0191 |
None | 0.0112 | 0.0006 | 0.0013 | 0.0206 | 0.0179 | 0.0064 | 0.0055 | 0.0013 | 0.001 | 0.0004 | 0.0008 | 0.0018 | 0.0063 | 0.0008 | 0.0021 | 0.0005 | 0.0008 | 0.002 |
Nuclear Periphery | 0.0667 | 0.0072 | 0.0138 | 0.0212 | 0.0143 | 0.0614 | 0.1017 | 0.0061 | 0.0216 | 0.0101 | 0.0225 | 0.0183 | 0.0644 | 0.0041 | 0.0124 | 0.0212 | 0.004 | 0.0129 |
Nucleolus | 0.0167 | 0.0009 | 0.0061 | 0.0158 | 0.0476 | 0.0034 | 0.0109 | 0.0051 | 0.003 | 0.0025 | 0.006 | 0.0018 | 0.0068 | 0.0022 | 0.0062 | 0.0026 | 0.0019 | 0.0044 |
Nucleus | 0.0216 | 0.005 | 0.0114 | 0.0133 | 0.0269 | 0.0253 | 0.0377 | 0.0083 | 0.0233 | 0.0145 | 0.0142 | 0.0185 | 0.0323 | 0.0059 | 0.0137 | 0.0244 | 0.0096 | 0.0209 |
Peroxisomes | 0.0005 | 0.0001 | 0.0002 | 0.0016 | 0.0016 | 0.0017 | 0.0005 | 0.0001 | 0.0002 | 0.0001 | 0.0003 | 0.0004 | 0.0005 | 0.0002 | 0.0008 | 0.0002 | 0.0002 | 0.0012 |
Punctate Nuclear | 0.0015 | 0.0001 | 0.0002 | 0.0007 | 0.0008 | 0.0166 | 0.0005 | 0.0001 | 0.0001 | 0.0001 | 0.0001 | 0.0001 | 0.0004 | 0 | 0.0001 | 0.0001 | 0.0001 | 0.0002 |
Vacuole | 0.1957 | 0.0946 | 0.1884 | 0.3703 | 0.1672 | 0.0971 | 0.1532 | 0.0651 | 0.1326 | 0.2007 | 0.2835 | 0.1196 | 0.1394 | 0.0569 | 0.0891 | 0.3568 | 0.391 | 0.1019 |
Vacuole Periphery | 0.0557 | 0.012 | 0.0369 | 0.1051 | 0.0824 | 0.0383 | 0.0561 | 0.0071 | 0.0333 | 0.04 | 0.203 | 0.0567 | 0.073 | 0.0156 | 0.0398 | 0.1502 | 0.1108 | 0.0783 |
R1 | R2 | |||||||||
---|---|---|---|---|---|---|---|---|---|---|
G1 Post-START | S/G2 | Metaphase | Anaphase | Telophase | G1 Post-START | S/G2 | Metaphase | Anaphase | Telophase | |
Gene Expression | 2821.8378 | 3204.5904 | 4036.053 | 2633.3475 | 3211.9803 | 3071.4169 | 2787.2869 | 2226.7566 | 2858.6844 | 2240.4867 |
Translational Efficiency | 0.981 | 0.9202 | 0.8132 | 1.2627 | 1.0651 | 0.9037 | 0.8931 | 1.4016 | 0.9334 | 1.1097 |
Dataset | Hit |
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Protein Concentration | ✘ |
Protein Localization | ✔ |
Gene Expression | ✘ |
Translational Efficiency | ✘ |
Temp | Actin Patch (Sac6-tdTomato) | Cortical Patch (Sla1-GFP) | Late Endosome (Snf7-GFP) | Vacuole (Vph1-GFP) |
---|---|---|---|---|
37℃ | ||||
RT |
Gene / Allele | Actin Patch (Sac6-tdTomato) | Cortical Patch (Sla1-GFP) | Late Endosome (Snf7-GFP) | Vacuole (Sac6-tdTomato) |
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Gene | Images |
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Gene | Images |
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