Standard name
Human Ortholog
Description Protein essential for meiotic DNA replication and sporulation; cytoplasmic protein; subunit of the MIS complex which controls mRNA methylation during during the induction of sporulation; also interacts with Orc2p, which is a component of the origin recognition complex
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Micrographs

ensLOC DeepLoc
Localization WT1 WT2 WT3 RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3 WT1 WT2 WT3 AF100 AF140 AF180
Cortical Patches 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Bud 0 0 0 0 0 0 0 0 0 0 0.05 0.05 0 0 0 0 0 0 0 0 0 0 0 0
Bud Neck 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Bud Site 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 – – – – – –
Cell Periphery 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0.14 0 0 0 0 0 0
Cytoplasm 0.96 0.99 0.96 0.99 0.97 0.92 0.83 0.83 0.77 0.75 0.67 0.76 0.99 0.99 0.99 0.93 0.98 0.97 0.92 0.93 0.93 0.76 0.73 0.66
Endoplasmic Reticulum 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0.05 0.07 0.08
Endosome 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Golgi 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Mitochondria 0.06 0 0 0 0 0.09 0.3 0.25 0.4 0.39 0.54 0.41 0 0 0 0 0 0 0 0 0 0.08 0.09 0.1
Nucleus 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0.05 0 0 0 0 0 0
Nuclear Periphery 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Nucleolus 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Peroxisomes 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
SpindlePole 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Vac/Vac Membrane 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
ensLOC DeepLoc
Localization WT1 WT2 WT3 RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3 WT1 WT2 WT3 AF100 AF140 AF180
Cortical Patches 0 0 0 0 0 1 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 3 3 4
Bud 2 0 0 0 2 2 4 3 5 5 13 13 2 0 0 0 0 0 0 1 0 0 1 1
Bud Neck 0 0 0 0 1 1 0 0 0 0 2 0 0 0 0 0 0 0 0 0 0 0 2 2
Bud Site 0 0 0 0 0 0 0 0 0 0 1 1 0 0 0 0 0 0 – – – – – –
Cell Periphery 0 1 0 4 2 5 2 2 8 1 3 2 2 4 1 1 2 5 0 0 0 0 0 0
Cytoplasm 166 144 78 170 272 184 223 210 225 221 183 183 231 263 341 25 44 36 158 141 96 127 200 189
Endoplasmic Reticulum 1 2 0 1 0 0 0 1 3 0 1 0 1 1 0 1 1 0 2 1 0 8 18 21
Endosome 0 0 0 0 0 0 0 1 0 1 0 0 0 1 3 0 0 0 2 0 0 5 6 9
Golgi 0 0 0 0 0 0 1 1 0 0 0 0 0 0 0 0 0 0 1 0 0 0 0 3
Mitochondria 11 2 1 0 12 18 82 62 118 114 148 99 0 1 0 1 1 1 4 1 2 13 24 27
Nucleus 1 0 3 2 2 1 3 0 3 3 0 4 0 0 1 0 0 2 0 2 0 0 0 0
Nuclear Periphery 0 0 0 0 0 0 0 0 1 0 0 1 0 0 0 1 0 0 0 0 0 0 0 0
Nucleolus 1 0 0 0 0 0 0 2 0 0 0 0 1 0 0 0 1 0 0 0 0 0 0 2
Peroxisomes 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
SpindlePole 0 0 0 0 0 1 0 1 0 2 1 1 0 0 0 0 0 0 0 0 0 0 2 4
Vac/Vac Membrane 0 0 0 1 2 3 5 9 6 7 6 8 0 1 0 0 1 0 0 1 1 2 10 11
Unique Cell Count 173 146 81 172 280 199 269 252 294 295 274 241 233 265 344 27 45 37 173 152 104 168 275 289
Labelled Cell Count 182 149 82 178 293 216 320 292 369 354 358 312 237 271 346 29 50 44 173 152 104 168 275 289


Cytoplasm

(Huh et al. Nature 2003 425:686)
Screen WT1 WT2 WT3 RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3 AF100 AF140 AF180
Mean Cell GFP Intensity (1e-4) 4.4 6.0 5.6 5.7 5.7 4.7 4.2 4.9 4.1 4.1 3.8 4.2 6.5 6.6 6.3 7.2 7.5 7.8 6.2 6.9 7.4
Std Deviation (1e-4) 0.6 0.8 1.1 1.6 1.6 1.4 1.3 1.9 1.5 0.9 1.0 1.3 1.3 1.3 1.4 1.0 1.0 1.6 1.5 1.8 1.9
Intensity Change (Log2) – – – 0.02 0.04 -0.26 -0.41 -0.2 -0.46 -0.45 -0.57 -0.42 0.21 0.24 0.18 0.37 0.42 0.48 0.16 0.3 0.4


Localization RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3
Cortical Patches 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Bud 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Bud Neck 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Bud Site 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Cell Periphery 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Cytoplasm 1.4 0.4 -1.2 -3.0 -3.0 -4.0 -4.2 -5.3 -4.0 1.8 1.9 1.9 -0.8 0.5 0.3
Endoplasmic Reticulum 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Endosome 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Golgi 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Mitochondria 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Nucleus 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Nuclear Periphery 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Nucleolus 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Peroxisomes 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
SpindlePole 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Vacuole 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0

Images

Circle plot not available

Protein Concentration and Protein Localization Data

R1 R2 R3
G1 Pre-START G1 Post-START S/G2 Metaphase Anaphase Telophase G1 Pre-START G1 Post-START S/G2 Metaphase Anaphase Telophase G1 Pre-START G1 Post-START S/G2 Metaphase Anaphase Telophase
Concentration 2.1612 2.9978 2.6595 2.0963 2.3978 2.7136 1.3547 2.1686 1.9779 1.7192 1.626 2.0099 0.638 1.4198 1.1246 1.272 1.0568 0.9898
Actin 0.0139 0.0006 0.0101 0.0002 0.0461 0.0061 0.0206 0.0012 0.0216 0.0044 0.0301 0.0037 0.0046 0.0004 0.0027 0.0076 0.0495 0.0032
Bud 0.0005 0.0061 0.0026 0.0001 0.0004 0.0028 0.0009 0.0028 0.0046 0.0002 0.0011 0.0004 0.0023 0.0001 0.0027 0.0045 0.0007 0.0002
Bud Neck 0.0005 0.0004 0.0008 0.0005 0.0006 0.0015 0.0103 0.0003 0.0006 0.0006 0.0026 0.001 0.0057 0.0001 0.0014 0.0025 0.0014 0.0016
Bud Periphery 0.0009 0.0032 0.0018 0 0.0004 0.0019 0.001 0.001 0.0041 0.0001 0.0023 0.0003 0.0026 0.0001 0.0028 0.0027 0.001 0.0002
Bud Site 0.0031 0.0151 0.0054 0.0001 0.0006 0.0004 0.005 0.0104 0.0084 0.0004 0.0017 0.0001 0.0032 0.0013 0.0058 0.003 0.0028 0.0001
Cell Periphery 0.0003 0.0002 0.0003 0 0.0001 0.0001 0.0003 0.0003 0.0002 0 0.0003 0 0.0002 0.0001 0.0002 0.0003 0.0002 0
Cytoplasm 0.6785 0.78 0.7852 0.7969 0.6546 0.7401 0.6861 0.7605 0.6924 0.6504 0.6657 0.7912 0.712 0.8159 0.7888 0.7779 0.8321 0.8775
Cytoplasmic Foci 0.0203 0.0123 0.0118 0.0122 0.029 0.0217 0.0201 0.0082 0.0235 0.0571 0.0209 0.0153 0.0177 0.0047 0.015 0.0132 0.0078 0.0147
Eisosomes 0.0002 0 0.0002 0 0.0002 0.0001 0.0002 0 0.0001 0 0.0006 0 0.0001 0.0001 0.0001 0.0002 0.0004 0
Endoplasmic Reticulum 0.0034 0.0019 0.0035 0.0025 0.0016 0.0017 0.0043 0.0017 0.003 0.0018 0.0042 0.0017 0.0049 0.0016 0.0033 0.0035 0.003 0.0026
Endosome 0.013 0.0062 0.009 0.0117 0.0215 0.0073 0.0213 0.0035 0.011 0.0097 0.0297 0.0096 0.018 0.0024 0.0086 0.0186 0.0178 0.0061
Golgi 0.0028 0.0005 0.0022 0.0008 0.008 0.0011 0.0051 0.0004 0.0062 0.0022 0.0155 0.0011 0.003 0.0002 0.0007 0.0015 0.0061 0.0007
Lipid Particles 0.0083 0.0001 0.0007 0 0.0043 0.0017 0.0052 0.0005 0.0071 0.0061 0.0245 0.0005 0.0016 0.0001 0.0008 0.0007 0.0083 0.0001
Mitochondria 0.0053 0.0004 0.0019 0.0003 0.0021 0.0007 0.0067 0.0004 0.0085 0.0009 0.0133 0.0062 0.0015 0.0001 0.0005 0.0023 0.003 0.0005
None 0.225 0.1685 0.1487 0.1703 0.2012 0.2038 0.1963 0.1988 0.1867 0.1765 0.1584 0.1605 0.1921 0.1702 0.1524 0.1267 0.0288 0.0855
Nuclear Periphery 0.0015 0.0005 0.0015 0.0006 0.0005 0.001 0.0028 0.0005 0.001 0.0012 0.0033 0.0006 0.0026 0.0004 0.0032 0.0078 0.0136 0.0038
Nucleolus 0.0007 0.0001 0.0007 0 0 0.0001 0.0005 0.0045 0.0002 0.0004 0.0004 0.0001 0.0091 0 0.0006 0.0017 0.0028 0
Nucleus 0.0017 0.0015 0.0041 0.0019 0.0008 0.0018 0.0026 0.002 0.0016 0.0022 0.0033 0.0015 0.0116 0.0008 0.0037 0.0088 0.0061 0.0013
Peroxisomes 0.0141 0.0004 0.0048 0.0001 0.0262 0.0029 0.0045 0.0003 0.0145 0.0595 0.0162 0.0019 0.001 0 0.0015 0.0004 0.003 0.0003
Punctate Nuclear 0.0042 0.0005 0.0024 0.0004 0.0009 0.0026 0.0035 0.0005 0.0029 0.0242 0.0032 0.0031 0.0029 0.0002 0.0021 0.0115 0.0094 0.0004
Vacuole 0.0015 0.0013 0.0019 0.001 0.0007 0.0006 0.0021 0.0019 0.0013 0.0017 0.0018 0.0007 0.0027 0.001 0.0024 0.0034 0.0012 0.0008
Vacuole Periphery 0.0005 0.0002 0.0004 0.0003 0.0003 0.0002 0.0006 0.0002 0.0004 0.0003 0.001 0.0002 0.0006 0.0001 0.0006 0.0012 0.0011 0.0003

Sequencing Data

R1 R2
G1 Post-START S/G2 Metaphase Anaphase Telophase G1 Post-START S/G2 Metaphase Anaphase Telophase
Gene Expression 16.0349 13.4384 11.3014 14.361 17.5416 13.0212 13.5875 12.2275 14.2015 16.7835
Translational Efficiency 1.4784 1.3466 1.3905 1.0057 1.307 2.0031 1.4561 1.5935 1.6136 1.3378

Hit Data

Dataset Hit
Protein Concentration ✔
Protein Localization ✘
Gene Expression ✔
Translational Efficiency ✘
Standard name
Human Ortholog
Description Protein essential for meiotic DNA replication and sporulation; cytoplasmic protein; subunit of the MIS complex which controls mRNA methylation during during the induction of sporulation; also interacts with Orc2p, which is a component of the origin recognition complex
Localization
Cell Percentages cytoplasm (84%)
Cell Cycle Regulation No
Subcompartmental Group cytoplasm-9

Mum2

Mum2


Nearest neighbours ( genes)

correlation threshold

0.6

1.0

0.80


Biological Process (Gene Ontology)
Molecular Function (Gene Ontology)
Cellular Component (Gene Ontology)

Endocytosis
Temp Actin Patch (Sac6-tdTomato) Cortical Patch (Sla1-GFP) Late Endosome (Snf7-GFP) Vacuole (Vph1-GFP)
37℃
RT
Cell Cycle Omics

CYCLoPs (Mum2-GFP)
Gene / Allele Actin Patch (Sac6-tdTomato) Cortical Patch (Sla1-GFP) Late Endosome (Snf7-GFP) Vacuole (Sac6-tdTomato)
Gene Images
Gene Images
Images are not yet available
Images are not yet available